Restriction map for
pF1KSDA0237
(Circular) (Six-base) MAPSORT of: pF1KSDA0237.seq Check: 2904 from: 1 to: 4063
pF1KSDA0237 4063 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 828 828
Size: 4063
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4063
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4063
AclI AA'CG_TT
Cuts at: 3903 3903
Size: 4063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1886 2318 3067 3335 362
Size: 1524 432 749 268 1090
Fragments arranged by size:
1524 1090 749 432 268
AflIII A'CryG_T
Cuts at: 1413 2519 3496 1413
Size: 1106 977 1980
Fragments arranged by size:
1980 1106 977
AgeI A'CCGG_T
Cuts at: 2451 2451
Size: 4063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 850 850
Size: 4063
AlwNI CAG_nnn'CTG
Cuts at: 148 579 801 1366 2935 148
Size: 431 222 565 1569 1276
Fragments arranged by size:
1569 1276 565 431 222
ApaLI G'TGCA_C
Cuts at: 604 2833 604
Size: 2229 1834
ApoI r'AATT_y
Cuts at: 1065 3416 3672 1065
Size: 2351 256 1456
Fragments arranged by size:
2351 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4063
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4063
AvaI C'yCGr_G
Cuts at: 659 1081 659
Size: 422 3641
BamHI G'GATC_C
Cuts at: 211 988 1086 211
Size: 777 98 3188
Fragments arranged by size:
3188 777 98
BanI G'GyrC_C
Cuts at: 274 1001 1077 1491 1739 1774 274
Size: 727 76 414 248 35 2563
Fragments arranged by size:
2563 727 414 248 76 35
BanII G_rGCy'C
Cuts at: 187 1075 2105 187
Size: 888 1030 2145
Fragments arranged by size:
2145 1030 888
BbeI G_GCGC'C
Cuts at: 1005 1495 1743 1005
Size: 490 248 3325
Fragments arranged by size:
3325 490 248
BbsI GAAGACnn'nnnn_
Cuts at: 760 778 1007 760
Size: 18 229 3816
Fragments arranged by size:
3816 229 18
BbvCI CC'TCA_GC
Cuts at: 922 922
Size: 4063
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2722 4063 1959
Size: 763 1341 1959
Fragments arranged by size:
1959 1341 763
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4063
BglI GCCn_nnn'nGGC
Cuts at: 742 742
Size: 4063
BglII A'GATC_T
Cuts at: 1576 3615 1576
Size: 2039 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4063
Bme1580I G_kGCm'C
Cuts at: 608 1686 1779 2837 608
Size: 1078 93 1058 1834
Fragments arranged by size:
1834 1078 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1005 1273 1354 1689 3835 1005
Size: 268 81 335 2146 1233
Fragments arranged by size:
2146 1233 335 268 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 171 310 658 745 1042 150
Size: 21 139 348 87 297 3171
Fragments arranged by size:
3171 348 297 139 87 21
Bpu10I CC'TnA_GC
Cuts at: 922 922
Size: 4063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2610 2908 1237
Size: 879 494 298 2392
Fragments arranged by size:
2392 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3767 3767
Size: 4063
BsaAI yAC'GTr
Cuts at: 1053 1414 1053
Size: 361 3702
BsaBI GATnn'nnATC
Cuts at: 6 1575 3614 6
Size: 1569 2039 455
Fragments arranged by size:
2039 1569 455
BsaHI Gr'CG_yC
Cuts at: 1002 1492 1740 2444 3940 1002
Size: 490 248 704 1496 1125
Fragments arranged by size:
1496 1125 704 490 248
BsaWI w'CCGG_w
Cuts at: 623 1250 1771 2451 2725 2872 3283 623
Size: 627 521 680 274 147 411 1403
Fragments arranged by size:
1403 680 627 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 954 954
Size: 4063
BseYI C'CCAG_C
Cuts at: 215 235 546 696 1486 2244 2823 3319 215
Size: 20 311 150 790 758 579 496 959
Fragments arranged by size:
959 790 758 579 496 311 150 20
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2859 104
Size: 1499 46 1210 1308
Fragments arranged by size:
1499 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 187 322 385 460 608 1075 1853 2043 2837
Size: 135 63 75 148 467 778 190 794
Cuts at: 2837 187
Size: 1413
Fragments arranged by size:
1413 794 778 467 190 148 135 75
63
Bsp1286I G_dGCh'C
Cuts at: 187 322 385 460 608 1075 1686 1779 1853
Size: 135 63 75 148 467 611 93 74
Cuts at: 1853 2043 2105 2837 187
Size: 190 62 732 1413
Fragments arranged by size:
1413 732 611 467 190 148 135 93
75 74 63 62
BspEI T'CCGG_A
Cuts at: 3283 3283
Size: 4063
BspHI T'CATG_A
Cuts at: 3239 3239
Size: 4063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 728 828 1111 1325 1627 2008 728
Size: 100 283 214 302 381 2783
Fragments arranged by size:
2783 381 302 283 214 100
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3893 4058 50
Size: 2303 54 1486 165 55
Fragments arranged by size:
2303 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 608 1973 608
Size: 1365 2698
BsrFI r'CCGG_y
Cuts at: 937 2059 2240 2451 937
Size: 1122 181 211 2549
Fragments arranged by size:
2549 1122 211 181
BssSI C'ACGA_G
Cuts at: 2332 2692 2332
Size: 360 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 801 1438 801
Size: 637 3426
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4063
BstEII G'GTnAC_C
Cuts at: 933 933
Size: 4063
BstXI CCAn_nnnn'nTGG
Cuts at: 174 285 174
Size: 111 3952
BstYI r'GATC_y
Cuts at: 1 211 988 1086 1553 1576 1911 2157 3171
Size: 210 777 98 467 23 335 246 1014
Cuts at: 3171 3257 3269 3615 1
Size: 86 12 346 449
Fragments arranged by size:
1014 777 467 449 346 335 246 210
98 86 23 12
BtgI C'CryG_G
Cuts at: 593 929 593
Size: 336 3727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 381 2011 2192 2355 381
Size: 1630 181 163 2089
Fragments arranged by size:
2089 1630 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4063
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4063
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2627 1767
Size: 860 3203
EaeI y'GGCC_r
Cuts at: 287 630 743 861 1646 1820 2211 2238 3585
Size: 343 113 118 785 174 391 27 1347
Cuts at: 3585 287
Size: 765
Fragments arranged by size:
1347 785 765 391 343 174 118 113
27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4063
EarI CTCTTCn'nnn_
Cuts at: 958 2084 2294 958
Size: 1126 210 2727
Fragments arranged by size:
2727 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 276 364 2269 2581 2727 3873 276
Size: 88 1905 312 146 1146 466
Fragments arranged by size:
1905 1146 466 312 146 88
EcoICRI GAG'CTC
Cuts at: 185 1073 185
Size: 888 3175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 171 310 362 658 745 1042 1886 2318
Size: 21 139 52 296 87 297 844 432
Cuts at: 2318 3067 3335 150
Size: 749 268 878
Fragments arranged by size:
878 844 749 432 297 296 268 139
87 52 21
EcoO109I rG'GnC_Cy
Cuts at: 145 161 663 734 817 1200 145
Size: 16 502 71 83 383 3008
Fragments arranged by size:
3008 502 383 83 71 16
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4063
FspI TGC'GCA
Cuts at: 364 1842 364
Size: 1478 2585
HaeII r_GCGC'y
Cuts at: 929 1005 1495 1743 2767 3746 929
Size: 76 490 248 1024 979 1246
Fragments arranged by size:
1246 1024 979 490 248 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 765 797 1903 1933 1935 1965 642
Size: 32 91 32 1106 30 2 30 2740
Fragments arranged by size:
2740 1106 91 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4063
KasI G'GCGC_C
Cuts at: 1001 1491 1739 1001
Size: 490 248 3325
Fragments arranged by size:
3325 490 248
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4063
MluI A'CGCG_T
Cuts at: 3496 3496
Size: 4063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 500 2734 2918 500
Size: 2234 184 1645
Fragments arranged by size:
2234 1645 184
MscI TGG'CCA
Cuts at: 289 632 745 1822 289
Size: 343 113 1077 2530
Fragments arranged by size:
2530 1077 343 113
MslI CAynn'nnrTG
Cuts at: 400 889 2050 2177 400
Size: 489 1161 127 2286
Fragments arranged by size:
2286 1161 489 127
MspA1I CmG'CkG
Cuts at: 636 931 1172 1486 1846 2861 3106 636
Size: 295 241 314 360 1015 245 1593
Fragments arranged by size:
1593 1015 360 314 295 245 241
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4063
NarI GG'CG_CC
Cuts at: 1002 1492 1740 1002
Size: 490 248 3325
Fragments arranged by size:
3325 490 248
NcoI C'CATG_G
Cuts at: 593 593
Size: 4063
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4063
NruI TCG'CGA
Cuts at: 2424 2424
Size: 4063
NspI r_CATG'y
Cuts at: 1114 1614 2523 1114
Size: 500 909 2654
Fragments arranged by size:
2654 909 500
PasI CC'CwG_GG
Cuts at: 822 822
Size: 4063
PciI A'CATG_T
Cuts at: 2519 2519
Size: 4063
PfoI T'CCnGG_A
Cuts at: 166 536 166
Size: 370 3693
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3234 3202
Size: 32 4031
PpuMI rG'GwC_Cy
Cuts at: 145 817 145
Size: 672 3391
PstI C_TGCA'G
Cuts at: 839 1108 1793 839
Size: 269 685 3109
Fragments arranged by size:
3109 685 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4063
PvuII CAG'CTG
Cuts at: 636 1486 1846 636
Size: 850 360 2853
Fragments arranged by size:
2853 850 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4063
SacI G_AGCT'C
Cuts at: 187 1075 187
Size: 888 3175
SacII CC_GC'GG
Cuts at: 932 932
Size: 4063
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4063
SanDI GG'GwC_CC
Cuts at: 817 817
Size: 4063
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3853
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4063
ScaI AGT'ACT
Cuts at: 3580 3580
Size: 4063
SfcI C'TryA_G
Cuts at: 33 835 1104 1789 2784 2975 33
Size: 802 269 685 995 191 1121
Fragments arranged by size:
1121 995 802 685 269 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 742 742
Size: 4063
SfoI GGC'GCC
Cuts at: 1003 1493 1741 1003
Size: 490 248 3325
Fragments arranged by size:
3325 490 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4063
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4063
SmlI C'TyrA_G
Cuts at: 1216 2131 2625 2887 1216
Size: 915 494 262 2392
Fragments arranged by size:
2392 915 494 262
SnaBI TAC'GTA
Cuts at: 1053 1053
Size: 4063
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4063
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3563
StyI C'CwwG_G
Cuts at: 593 1195 1549 593
Size: 602 354 3107
Fragments arranged by size:
3107 602 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 606 1644 1777 2236 2447 262
Size: 344 1038 133 459 211 1878
Fragments arranged by size:
1878 1038 459 344 211 133
TatI w'GTAC_w
Cuts at: 3578 3578
Size: 4063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 862 894 3580 3612 862
Size: 32 2686 32 1313
Fragments arranged by size:
2686 1313 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4063
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 966 1556 966
Size: 590 3473
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4063
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SexAI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI