Restriction map for
pF1KSDA0245
(Circular) (Six-base) MAPSORT of: pF1KSDA0245.seq Check: 9386 from: 1 to: 4682
pF1KSDA0245 4682 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1720 1720
Size: 4682
Acc65I G'GTAC_C
Cuts at: 1698 1698
Size: 4682
AclI AA'CG_TT
Cuts at: 4522 4522
Size: 4682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1287 1293 2507 2939 3686 3954 268
Size: 1019 6 1214 432 747 268 996
Fragments arranged by size:
1214 1019 996 747 432 268 6
AfeI AGC'GCT
Cuts at: 731 731
Size: 4682
AflIII A'CryG_T
Cuts at: 691 693 704 2034 3138 4115 691
Size: 2 11 1330 1104 977 1258
Fragments arranged by size:
1330 1258 1104 977 11 2
AgeI A'CCGG_T
Cuts at: 538 3070 538
Size: 2532 2150
AhdI GACnn_n'nnGTC
Cuts at: 1191 1191
Size: 4682
AleI CACnn'nnGTG
Cuts at: 694 694
Size: 4682
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 667 712 667
Size: 45 4637
AlwNI CAG_nnn'CTG
Cuts at: 1316 1987 3554 1316
Size: 671 1567 2444
Fragments arranged by size:
2444 1567 671
ApaLI G'TGCA_C
Cuts at: 3452 3452
Size: 4682
ApoI r'AATT_y
Cuts at: 921 1686 4035 4291 921
Size: 765 2349 256 1312
Fragments arranged by size:
2349 1312 765 256
AseI AT'TA_AT
Cuts at: 20 1472 20
Size: 1452 3230
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
AvaI C'yCGr_G
Cuts at: 1166 1702 1166
Size: 536 4146
BamHI G'GATC_C
Cuts at: 1707 1707
Size: 4682
BanI G'GyrC_C
Cuts at: 406 1594 1698 2112 2360 2395 406
Size: 1188 104 414 248 35 2693
Fragments arranged by size:
2693 1188 414 248 104 35
BanII G_rGCy'C
Cuts at: 392 536 1165 1373 1696 2726 392
Size: 144 629 208 323 1030 2348
Fragments arranged by size:
2348 1030 629 323 208 144
BbeI G_GCGC'C
Cuts at: 2116 2364 2116
Size: 248 4434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 282 316 282
Size: 34 4648
BciVI GTATCCnnnnn_n'
Cuts at: 2580 3341 4682 2580
Size: 761 1341 2580
Fragments arranged by size:
2580 1341 761
BclI T'GATC_A
Cuts at: 2202 2202
Size: 4682
BfrBI ATG'CAT
Cuts at: 1126 1126
Size: 4682
BglI GCCn_nnn'nGGC
Cuts at: 147 1377 147
Size: 1230 3452
BglII A'GATC_T
Cuts at: 257 2197 4234 257
Size: 1940 2037 705
Fragments arranged by size:
2037 1940 705
BlpI GC'TnA_GC
Cuts at: 1396 1794 1396
Size: 398 4284
Bme1580I G_kGCm'C
Cuts at: 411 691 1454 2307 2400 3456 411
Size: 280 763 853 93 1056 1637
Fragments arranged by size:
1637 1056 853 763 280 93
BmrI ACTGGGnnnn_n'
Cuts at: 1894 1975 2310 4454 1894
Size: 81 335 2144 2122
Fragments arranged by size:
2144 2122 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 828 3049 828
Size: 2221 2461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1526
Size: 4682
Bpu10I CC'TnA_GC
Cuts at: 306 1021 306
Size: 715 3967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1858 2737 3229 3527 1375
Size: 483 879 492 298 2530
Fragments arranged by size:
2530 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 519 4386 519
Size: 3867 815
BsaAI yAC'GTr
Cuts at: 694 1674 2035 694
Size: 980 361 3341
Fragments arranged by size:
3341 980 361
BsaBI GATnn'nnATC
Cuts at: 6 2196 4233 6
Size: 2190 2037 455
Fragments arranged by size:
2190 2037 455
BsaHI Gr'CG_yC
Cuts at: 797 2113 2361 3063 4559 797
Size: 1316 248 702 1496 920
Fragments arranged by size:
1496 1316 920 702 248
BsaWI w'CCGG_w
Cuts at: 538 1182 1871 2392 3070 3344 3491 3902 538
Size: 644 689 521 678 274 147 411 1318
Fragments arranged by size:
1318 689 678 644 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 334
Size: 30 4652
BseYI C'CCAG_C
Cuts at: 426 595 1598 2107 2865 3442 3938 426
Size: 169 1003 509 758 577 496 1170
Fragments arranged by size:
1170 1003 758 577 509 496 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 718
Size: 4682
BsiEI CG_ry'CG
Cuts at: 104 2224 2270 3478 104
Size: 2120 46 1208 1308
Fragments arranged by size:
2120 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 788 1696 2474 2664 3456 788
Size: 908 778 190 792 2014
Fragments arranged by size:
2014 908 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 631 631
Size: 4682
Bsp1286I G_dGCh'C
Cuts at: 392 411 536 691 788 1165 1373 1454 1696
Size: 19 125 155 97 377 208 81 242
Cuts at: 1696 2307 2400 2474 2664 2726 3456 392
Size: 611 93 74 190 62 730 1618
Fragments arranged by size:
1618 730 611 377 242 208 190 155
125 97 93 81 74 62 19
BspEI T'CCGG_A
Cuts at: 3902 3902
Size: 4682
BspHI T'CATG_A
Cuts at: 3858 3858
Size: 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1544 1732 1946 2248 2629 1544
Size: 188 214 302 381 3597
Fragments arranged by size:
3597 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 2974 3028 4512 4677 50
Size: 2924 54 1484 165 55
Fragments arranged by size:
2924 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 491 734 1088 2594 491
Size: 243 354 1506 2579
Fragments arranged by size:
2579 1506 354 243
BsrFI r'CCGG_y
Cuts at: 538 2680 2861 3070 538
Size: 2142 181 209 2150
Fragments arranged by size:
2150 2142 209 181
BssSI C'ACGA_G
Cuts at: 2953 3311 2953
Size: 358 4324
BstAPI GCAn_nnn'nTGC
Cuts at: 2059 2059
Size: 4682
BstBI TT'CG_AA
Cuts at: 110 3043 110
Size: 2933 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1063 1063
Size: 4682
BstYI r'GATC_y
Cuts at: 1 257 311 1707 2174 2197 2532 2778 3790
Size: 256 54 1396 467 23 335 246 1012
Cuts at: 3790 3876 3888 4234 1
Size: 86 12 346 449
Fragments arranged by size:
1396 1012 467 449 346 335 256 246
86 54 23 12
BtgI C'CryG_G
Cuts at: 125 1250 125
Size: 1125 3557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 537 2632 2813 2976 537
Size: 2095 181 163 2243
Fragments arranged by size:
2243 2095 181 163
BtsI GCAGTG_nn'
Cuts at: 344 517 1032 1925 344
Size: 173 515 893 3101
Fragments arranged by size:
3101 893 515 173
DraI TTT'AAA
Cuts at: 1681 1681
Size: 4682
DrdI GACnn_nn'nnGTC
Cuts at: 2388 3246 2388
Size: 858 3824
EaeI y'GGCC_r
Cuts at: 932 2267 2441 2832 2859 4204 932
Size: 1335 174 391 27 1345 1410
Fragments arranged by size:
1410 1345 1335 391 174 27
EagI C'GGCC_G
Cuts at: 2267 2267
Size: 4682
EarI CTCTTCn'nnn_
Cuts at: 397 862 1462 2705 2915 397
Size: 465 600 1243 210 2164
Fragments arranged by size:
2164 1243 600 465 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 493 2890 3200 3346 4492 493
Size: 2397 310 146 1146 683
Fragments arranged by size:
2397 1146 683 310 146
EcoICRI GAG'CTC
Cuts at: 1694 1694
Size: 4682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1287 1293 1526 2507 2939 3686 3954 268
Size: 1019 6 233 981 432 747 268 996
Fragments arranged by size:
1019 996 981 747 432 268 233 6
EcoO109I rG'GnC_Cy
Cuts at: 227 1116 1821 227
Size: 889 705 3088
Fragments arranged by size:
3088 889 705
EcoRI G'AATT_C
Cuts at: 1686 1686
Size: 4682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4682
FspI TGC'GCA
Cuts at: 2463 2463
Size: 4682
HaeII r_GCGC'y
Cuts at: 733 2116 2364 3386 4365 733
Size: 1383 248 1022 979 1050
Fragments arranged by size:
1383 1050 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 1177 1209 2524 2554 2556 2586 196
Size: 32 949 32 1315 30 2 30 2292
Fragments arranged by size:
2292 1315 949 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1721 1721
Size: 4682
KasI G'GCGC_C
Cuts at: 2112 2360 2112
Size: 248 4434
KpnI G_GTAC'C
Cuts at: 1702 1702
Size: 4682
MfeI C'AATT_G
Cuts at: 953 1242 953
Size: 289 4393
MluI A'CGCG_T
Cuts at: 4115 4115
Size: 4682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1323 3353 3537 1323
Size: 2030 184 2468
Fragments arranged by size:
2468 2030 184
MscI TGG'CCA
Cuts at: 934 2443 934
Size: 1509 3173
MslI CAynn'nnrTG
Cuts at: 694 748 2671 2798 694
Size: 54 1923 127 2578
Fragments arranged by size:
2578 1923 127 54
MspA1I CmG'CkG
Cuts at: 246 599 1076 1361 1793 2107 2467 3480 3725
Size: 353 477 285 432 314 360 1013 245
Cuts at: 3725 246
Size: 1203
Fragments arranged by size:
1203 1013 477 432 360 353 314 285
245
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 4682
NarI GG'CG_CC
Cuts at: 2113 2361 2113
Size: 248 4434
NcoI C'CATG_G
Cuts at: 125 1250 125
Size: 1125 3557
NdeI CA'TA_TG
Cuts at: 646 1426 646
Size: 780 3902
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 4682
NsiI A_TGCA'T
Cuts at: 1128 1128
Size: 4682
NspI r_CATG'y
Cuts at: 1071 1735 2235 3142 1071
Size: 664 500 907 2611
Fragments arranged by size:
2611 907 664 500
PciI A'CATG_T
Cuts at: 3138 3138
Size: 4682
PfoI T'CCnGG_A
Cuts at: 1546 1546
Size: 4682
PmeI GTTT'AAAC
Cuts at: 1681 1681
Size: 4682
PmlI CAC'GTG
Cuts at: 694 694
Size: 4682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 4650
PpuMI rG'GwC_Cy
Cuts at: 227 227
Size: 4682
PstI C_TGCA'G
Cuts at: 1729 2414 1729
Size: 685 3997
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4682
PvuII CAG'CTG
Cuts at: 246 599 1076 2107 2467 246
Size: 353 477 1031 360 2461
Fragments arranged by size:
2461 1031 477 360 353
RsrII CG'GwC_CG
Cuts at: 2877 2877
Size: 4682
SacI G_AGCT'C
Cuts at: 1696 1696
Size: 4682
SalI G'TCGA_C
Cuts at: 1719 1719
Size: 4682
SapI GCTCTTCn'nnn_
Cuts at: 397 2705 2915 397
Size: 2308 210 2164
Fragments arranged by size:
2308 2164 210
SbfI CC_TGCA'GG
Cuts at: 1729 1729
Size: 4682
ScaI AGT'ACT
Cuts at: 4199 4199
Size: 4682
SfcI C'TryA_G
Cuts at: 33 1725 2410 3403 3594 33
Size: 1692 685 993 191 1121
Fragments arranged by size:
1692 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2114 2362 2114
Size: 248 4434
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4682
SmaI CCC'GGG
Cuts at: 1168 1704 1168
Size: 536 4146
SmlI C'TyrA_G
Cuts at: 1390 1837 2752 3244 3506 1390
Size: 447 915 492 262 2566
Fragments arranged by size:
2566 915 492 447 262
SnaBI TAC'GTA
Cuts at: 1674 1674
Size: 4682
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4682
SphI G_CATG'C
Cuts at: 1735 2235 1735
Size: 500 4182
StuI AGG'CCT
Cuts at: 478 478
Size: 4682
StyI C'CwwG_G
Cuts at: 125 326 719 929 1250 1816 2170 125
Size: 201 393 210 321 566 354 2637
Fragments arranged by size:
2637 566 393 354 321 210 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 171 339 394 1516 1612 2265 2398 2857 3066
Size: 168 55 1122 96 653 133 459 209
Cuts at: 3066 171
Size: 1787
Fragments arranged by size:
1787 1122 653 459 209 168 133 96
55
TatI w'GTAC_w
Cuts at: 4197 4197
Size: 4682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 4199 4231 334
Size: 32 3833 32 785
Fragments arranged by size:
3833 785 32 32
Tth111I GACn'n_nGTC
Cuts at: 2479 2479
Size: 4682
XbaI T'CTAG_A
Cuts at: 471 1713 471
Size: 1242 3440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 747 2177 333
Size: 414 1430 2838
Fragments arranged by size:
2838 1430 414
XmaI C'CCGG_G
Cuts at: 1166 1702 1166
Size: 536 4146
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BbsI BbvCI BmgBI BmtI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI XmnI ZraI