Restriction map for
pF1KSDA0248
(Circular) (Six-base) MAPSORT of: pF1KSDA0248.seq Check: 833 from: 1 to: 8716
pF1KSDA0248 8716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5568 5568
Size: 8716
AccI GT'mk_AC
Cuts at: 683 4091 5752 683
Size: 3408 1661 3647
Fragments arranged by size:
3647 3408 1661
Acc65I G'GTAC_C
Cuts at: 5730 5730
Size: 8716
AclI AA'CG_TT
Cuts at: 8556 8556
Size: 8716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 520 1208 2421 2982 3895 5158 6539 6971
Size: 152 688 1213 561 913 1263 1381 432
Cuts at: 6971 7720 7988 368
Size: 749 268 1096
Fragments arranged by size:
1381 1263 1213 1096 913 749 688 561
432 268 152
AflII C'TTAA_G
Cuts at: 4294 4294
Size: 8716
AflIII A'CryG_T
Cuts at: 2747 6066 7172 8149 2747
Size: 3319 1106 977 3314
Fragments arranged by size:
3319 3314 1106 977
AgeI A'CCGG_T
Cuts at: 7104 7104
Size: 8716
AhdI GACnn_n'nnGTC
Cuts at: 4284 5112 4284
Size: 828 7888
AleI CACnn'nnGTG
Cuts at: 3680 3902 3680
Size: 222 8494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2884 4787 2884
Size: 1903 6813
AlwNI CAG_nnn'CTG
Cuts at: 469 967 1093 1171 1450 2286 2451 3786 4818
Size: 498 126 78 279 836 165 1335 1032
Cuts at: 4818 5016 6019 7588 469
Size: 198 1003 1569 1597
Fragments arranged by size:
1597 1569 1335 1032 1003 836 498 279
198 165 126 78
ApaI G_GGCC'C
Cuts at: 2923 3412 4014 4208 4743 2923
Size: 489 602 194 535 6896
Fragments arranged by size:
6896 602 535 489 194
ApaLI G'TGCA_C
Cuts at: 1203 7486 1203
Size: 6283 2433
ApoI r'AATT_y
Cuts at: 192 1009 3073 5718 8069 8325 192
Size: 817 2064 2645 2351 256 583
Fragments arranged by size:
2645 2351 2064 817 583 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8716
AvaI C'yCGr_G
Cuts at: 1117 1265 1982 3833 4535 5437 5447 5734 1117
Size: 148 717 1851 702 902 10 287 4099
Fragments arranged by size:
4099 1851 902 717 702 287 148 10
BamHI G'GATC_C
Cuts at: 234 498 3288 5739 234
Size: 264 2790 2451 3211
Fragments arranged by size:
3211 2790 2451 264
BanI G'GyrC_C
Cuts at: 571 1978 2845 4751 4796 5730 6144 6392 6427
Size: 1407 867 1906 45 934 414 248 35
Cuts at: 6427 571
Size: 2860
Fragments arranged by size:
2860 1906 1407 934 867 414 248 45
35
BanII G_rGCy'C
Cuts at: 308 1346 1739 2923 3246 3412 3527 3863 4014
Size: 1038 393 1184 323 166 115 336 151
Cuts at: 4014 4031 4208 4364 4743 5446 5509 5728 6758
Size: 17 177 156 379 703 63 219 1030
Cuts at: 6758 308
Size: 2266
Fragments arranged by size:
2266 1184 1038 1030 703 393 379 336
323 219 177 166 156 151 115 63
17
BbeI G_GCGC'C
Cuts at: 4800 6148 6396 4800
Size: 1348 248 7120
Fragments arranged by size:
7120 1348 248
BbsI GAAGACnn'nnnn_
Cuts at: 1043 1318 3883 4995 1043
Size: 275 2565 1112 4764
Fragments arranged by size:
4764 2565 1112 275
BbvCI CC'TCA_GC
Cuts at: 972 3034 5337 972
Size: 2062 2303 4351
Fragments arranged by size:
4351 2303 2062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3640 3674 3640
Size: 34 8682
BciVI GTATCCnnnnn_n'
Cuts at: 6612 7375 8716 6612
Size: 763 1341 6612
Fragments arranged by size:
6612 1341 763
BclI T'GATC_A
Cuts at: 3482 4232 6234 3482
Size: 750 2002 5964
Fragments arranged by size:
5964 2002 750
BglI GCCn_nnn'nGGC
Cuts at: 4217 4217
Size: 8716
BglII A'GATC_T
Cuts at: 3302 6229 8268 3302
Size: 2927 2039 3750
Fragments arranged by size:
3750 2927 2039
BlpI GC'TnA_GC
Cuts at: 3355 5503 5537 5826 3355
Size: 2148 34 289 6245
Fragments arranged by size:
6245 2148 289 34
Bme1580I G_kGCm'C
Cuts at: 439 496 576 938 1207 2923 3412 4014 4208
Size: 57 80 362 269 1716 489 602 194
Cuts at: 4208 4743 4756 5651 6339 6432 7490 439
Size: 535 13 895 688 93 1058 1665
Fragments arranged by size:
1716 1665 1058 895 688 602 535 489
362 269 194 93 80 57 13
BmgBI CAC'GTC
Cuts at: 1208 4053 1208
Size: 2845 5871
BmrI ACTGGGnnnn_n'
Cuts at: 559 1155 1452 3107 3815 3921 4008 5926 6007
Size: 596 297 1655 708 106 87 1918 81
Cuts at: 6007 6342 8488 559
Size: 335 2146 787
Fragments arranged by size:
2146 1918 1655 787 708 596 335 297
106 87 81
BmtI G_CTAG'C
Cuts at: 1994 1994
Size: 8716
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 842 1612 5287 5655 7081 842
Size: 770 3675 368 1426 2477
Fragments arranged by size:
3675 2477 1426 770 368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1117 1711 2137 2551 2698 2761 3487 3529
Size: 65 594 426 414 147 63 726 42
Cuts at: 3529 3947 3955 4503 4714 5085 1052
Size: 418 8 548 211 371 4683
Fragments arranged by size:
4683 726 594 548 426 418 414 371
211 147 65 63 42 8
Bpu10I CC'TnA_GC
Cuts at: 972 3034 3389 3607 3779 3948 5337 972
Size: 2062 355 218 172 169 1389 4351
Fragments arranged by size:
4351 2062 1389 355 218 172 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 1582 2983 5300 5472 5890 6769 7263 7561
Size: 123 1401 2317 172 418 879 494 298
Cuts at: 7561 1459
Size: 2614
Fragments arranged by size:
2614 2317 1401 879 494 418 298 172
123
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3656 4960 8420 3546
Size: 110 1304 3460 3842
Fragments arranged by size:
3842 3460 1304 110
BsaAI yAC'GTr
Cuts at: 5706 6067 5706
Size: 361 8355
BsaBI GATnn'nnATC
Cuts at: 6 529 1618 6228 8267 6
Size: 523 1089 4610 2039 455
Fragments arranged by size:
4610 2039 1089 523 455
BsaHI Gr'CG_yC
Cuts at: 4797 6145 6393 7097 8593 4797
Size: 1348 248 704 1496 4920
Fragments arranged by size:
4920 1496 1348 704 248
BsaWI w'CCGG_w
Cuts at: 620 3758 4379 5903 6424 7104 7378 7525 7936
Size: 3138 621 1524 521 680 274 147 411
Cuts at: 7936 620
Size: 1400
Fragments arranged by size:
3138 1524 1400 680 621 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1192 1222 2372 2402 2734 2764 5083 5113 1192
Size: 30 1150 30 332 30 2319 30 4795
Fragments arranged by size:
4795 2319 1150 332 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 939 1005 1213 2293 2695 2803 2980 3353 4693
Size: 66 208 1080 402 108 177 373 1340
Cuts at: 4693 4978 939
Size: 285 4677
Fragments arranged by size:
4677 1340 1080 402 373 285 208 177
108 66
BseYI C'CCAG_C
Cuts at: 1720 1928 4207 5412 5494 6139 6897 7476 7972
Size: 208 2279 1205 82 645 758 579 496
Cuts at: 7972 1720
Size: 2464
Fragments arranged by size:
2464 2279 1205 758 645 579 496 208
82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1723 4624 5204 712
Size: 1011 2901 580 4224
Fragments arranged by size:
4224 2901 1011 580
BsiEI CG_ry'CG
Cuts at: 104 1383 6256 6302 7512 104
Size: 1279 4873 46 1210 1308
Fragments arranged by size:
4873 1308 1279 1210 46
BsiHKAI G_wGCw'C
Cuts at: 208 674 1207 1346 1595 1739 2434 3527 3780
Size: 466 533 139 249 144 695 1093 253
Cuts at: 3780 4031 4364 4839 5728 6506 6696 7490 208
Size: 251 333 475 889 778 190 794 1434
Fragments arranged by size:
1434 1093 889 794 778 695 533 475
466 333 253 251 249 190 144 139
BsmI GAATG_Cn'
Cuts at: 1815 2536 2667 1815
Size: 721 131 7864
Fragments arranged by size:
7864 721 131
Bsp1286I G_dGCh'C
Cuts at: 208 308 439 496 576 674 938 1207 1346
Size: 100 131 57 80 98 264 269 139
Cuts at: 1346 1595 1739 2434 2923 3246 3412 3527 3780
Size: 249 144 695 489 323 166 115 253
Cuts at: 3780 3863 4014 4031 4208 4364 4743 4756 4839
Size: 83 151 17 177 156 379 13 83
Cuts at: 4839 5446 5509 5651 5728 6339 6432 6506 6696
Size: 607 63 142 77 611 93 74 190
Cuts at: 6696 6758 7490 208
Size: 62 732 1434
Fragments arranged by size:
1434 732 695 611 607 489 379 323
269 264 253 249 190 177 166 156
151 144 142 139 131 115 100 98
93 83 83 80 77 74 63 62
57 17 13
BspEI T'CCGG_A
Cuts at: 620 3758 4379 7936 620
Size: 3138 621 3557 1400
Fragments arranged by size:
3557 3138 1400 621
BspHI T'CATG_A
Cuts at: 3527 7892 3527
Size: 4365 4351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3116 5568 5764 5978 6280 6661 3116
Size: 2452 196 214 302 381 5171
Fragments arranged by size:
5171 2452 381 302 214 196
BsrBI CCG'CTC
Cuts at: 50 3717 7006 7060 8546 8711 50
Size: 3667 3289 54 1486 165 55
Fragments arranged by size:
3667 3289 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3469 4987 6626 3469
Size: 1518 1639 5559
Fragments arranged by size:
5559 1639 1518
BsrFI r'CCGG_y
Cuts at: 4505 6712 6893 7104 4505
Size: 2207 181 211 6117
Fragments arranged by size:
6117 2207 211 181
BsrGI T'GTAC_A
Cuts at: 1616 4845 1616
Size: 3229 5487
BssSI C'ACGA_G
Cuts at: 6985 7345 6985
Size: 360 8356
BstAPI GCAn_nnn'nTGC
Cuts at: 4003 4638 6091 4003
Size: 635 1453 6628
Fragments arranged by size:
6628 1453 635
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8716
BstEII G'GTnAC_C
Cuts at: 710 710
Size: 8716
BstXI CCAn_nnnn'nTGG
Cuts at: 2316 2676 3181 3441 4926 5307 2316
Size: 360 505 260 1485 381 5725
Fragments arranged by size:
5725 1485 505 381 360 260
BstYI r'GATC_y
Cuts at: 1 234 420 498 623 820 2190 3288 3302
Size: 233 186 78 125 197 1370 1098 14
Cuts at: 3302 4382 5676 5739 6206 6229 6564 6810 7824
Size: 1080 1294 63 467 23 335 246 1014
Cuts at: 7824 7910 7922 8268 1
Size: 86 12 346 449
Fragments arranged by size:
1370 1294 1098 1080 1014 467 449 346
335 246 233 197 186 125 86 78
63 23 14 12
Bsu36I CC'TnA_GG
Cuts at: 2786 2855 2786
Size: 69 8647
BtgI C'CryG_G
Cuts at: 125 2194 4101 5306 125
Size: 2069 1907 1205 3535
Fragments arranged by size:
3535 2069 1907 1205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2030 2600 3053 4802 6664 6845 7008 2030
Size: 570 453 1749 1862 181 163 3738
Fragments arranged by size:
3738 1862 1749 570 453 181 163
BtsI GCAGTG_nn'
Cuts at: 983 1107 1896 2115 3096 3728 3928 4743 5023
Size: 124 789 219 981 632 200 815 280
Cuts at: 5023 5572 5957 983
Size: 549 385 3742
Fragments arranged by size:
3742 981 815 789 632 549 385 280
219 200 124
DraI TTT'AAA
Cuts at: 275 723 5713 275
Size: 448 4990 3278
Fragments arranged by size:
4990 3278 448
DraIII CAC_nnn'GTG
Cuts at: 3382 3382
Size: 8716
DrdI GACnn_nn'nnGTC
Cuts at: 4087 6420 7280 4087
Size: 2333 860 5523
Fragments arranged by size:
5523 2333 860
EaeI y'GGCC_r
Cuts at: 3743 4704 5379 5657 6299 6473 6864 6891 8238
Size: 961 675 278 642 174 391 27 1347
Cuts at: 8238 3743
Size: 4221
Fragments arranged by size:
4221 1347 961 675 642 391 278 174
27
EagI C'GGCC_G
Cuts at: 6299 6299
Size: 8716
EarI CTCTTCn'nnn_
Cuts at: 706 811 1351 2908 3241 3314 3554 3761 3928
Size: 105 540 1557 333 73 240 207 167
Cuts at: 3928 5278 6737 6947 706
Size: 1350 1459 210 2475
Fragments arranged by size:
2475 1557 1459 1350 540 333 240 210
207 167 105 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 3009 4424 4690 6922 7234 7380 8526 2461
Size: 548 1415 266 2232 312 146 1146 2651
Fragments arranged by size:
2651 2232 1415 1146 548 312 266 146
EcoICRI GAG'CTC
Cuts at: 1344 1737 3525 4029 4362 5726 1344
Size: 393 1788 504 333 1364 4334
Fragments arranged by size:
4334 1788 1364 504 393 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 520 1052 1117 1208 1711 2137 2421 2551
Size: 152 532 65 91 503 426 284 130
Cuts at: 2551 2698 2761 2982 3487 3529 3895 3947 3955
Size: 147 63 221 505 42 366 52 8
Cuts at: 3955 4503 4714 5085 5158 6539 6971 7720 7988
Size: 548 211 371 73 1381 432 749 268
Cuts at: 7988 368
Size: 1096
Fragments arranged by size:
1381 1096 749 548 532 505 503 432
426 371 366 284 268 221 211 152
147 130 91 73 65 63 52 42
8
EcoNI CCTnn'n_nnAGG
Cuts at: 1483 1780 3196 1483
Size: 297 1416 7003
Fragments arranged by size:
7003 1416 297
EcoO109I rG'GnC_Cy
Cuts at: 1142 2919 2920 3408 4010 4204 4219 5295 5417
Size: 1777 1 488 602 194 15 1076 122
Cuts at: 5417 5853 1142
Size: 436 4005
Fragments arranged by size:
4005 1777 1076 602 488 436 194 122
15 1
EcoRI G'AATT_C
Cuts at: 1009 5718 1009
Size: 4709 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1830 6733 1830
Size: 4903 3813
FspI TGC'GCA
Cuts at: 3799 6495 3799
Size: 2696 6020
HaeII r_GCGC'y
Cuts at: 4800 6148 6396 7420 8399 4800
Size: 1348 248 1024 979 5117
Fragments arranged by size:
5117 1348 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 365 397 1192 1219 1224 1251 1485 1517 3313
Size: 32 795 27 5 27 234 32 1796
Cuts at: 3313 3345 3755 3762 3787 3794 4018 4047 4050
Size: 32 410 7 25 7 224 29 3
Cuts at: 4050 4079 4270 4302 5098 5130 5389 5421 6556
Size: 29 191 32 796 32 259 32 1135
Cuts at: 6556 6586 6588 6618 365
Size: 30 2 30 2463
Fragments arranged by size:
2463 1796 1135 796 795 410 259 234
224 191 32 32 32 32 32 32
30 30 29 29 27 27 25 7
7 5 3 2
HincII GTy'rAC
Cuts at: 393 1831 2130 3347 4152 5700 5753 393
Size: 1438 299 1217 805 1548 53 3356
Fragments arranged by size:
3356 1548 1438 1217 805 299 53
HindIII A'AGCT_T
Cuts at: 1627 1627
Size: 8716
KasI G'GCGC_C
Cuts at: 4796 6144 6392 4796
Size: 1348 248 7120
Fragments arranged by size:
7120 1348 248
KpnI G_GTAC'C
Cuts at: 5734 5734
Size: 8716
MluI A'CGCG_T
Cuts at: 8149 8149
Size: 8716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1796 2396 2530 3599 4103 7387 7571 1796
Size: 600 134 1069 504 3284 184 2941
Fragments arranged by size:
3284 2941 1069 600 504 184 134
MscI TGG'CCA
Cuts at: 3745 4706 5381 5659 6475 3745
Size: 961 675 278 816 5986
Fragments arranged by size:
5986 961 816 675 278
MslI CAynn'nnrTG
Cuts at: 801 931 1645 2314 2843 3179 3680 3902 4046
Size: 130 714 669 529 336 501 222 144
Cuts at: 4046 4448 5305 6703 6830 801
Size: 402 857 1398 127 2687
Fragments arranged by size:
2687 1398 857 714 669 529 501 402
336 222 144 130 127
MspA1I CmG'CkG
Cuts at: 696 3315 3507 3967 5167 5356 5825 6139 6499
Size: 2619 192 460 1200 189 469 314 360
Cuts at: 6499 7514 7759 696
Size: 1015 245 1653
Fragments arranged by size:
2619 1653 1200 1015 469 460 360 314
245 192 189
NaeI GCC'GGC
Cuts at: 4507 6895 4507
Size: 2388 6328
NarI GG'CG_CC
Cuts at: 4797 6145 6393 4797
Size: 1348 248 7120
Fragments arranged by size:
7120 1348 248
NcoI C'CATG_G
Cuts at: 125 4101 5306 125
Size: 3976 1205 3535
Fragments arranged by size:
3976 3535 1205
NdeI CA'TA_TG
Cuts at: 842 3567 842
Size: 2725 5991
NgoMIV G'CCGG_C
Cuts at: 4505 6893 4505
Size: 2388 6328
NheI G'CTAG_C
Cuts at: 1990 1990
Size: 8716
NruI TCG'CGA
Cuts at: 7077 7077
Size: 8716
NspI r_CATG'y
Cuts at: 921 1587 2751 4531 5091 5148 5767 6267 7176
Size: 666 1164 1780 560 57 619 500 909
Cuts at: 7176 921
Size: 2461
Fragments arranged by size:
2461 1780 1164 909 666 619 560 500
57
PciI A'CATG_T
Cuts at: 2747 7172 2747
Size: 4425 4291
PflMI CCAn_nnn'nTGG
Cuts at: 1248 2092 2915 1248
Size: 844 823 7049
Fragments arranged by size:
7049 844 823
PfoI T'CCnGG_A
Cuts at: 1133 2738 5291 1133
Size: 1605 2553 4558
Fragments arranged by size:
4558 2553 1605
PmeI GTTT'AAAC
Cuts at: 5713 5713
Size: 8716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7855 7887 7855
Size: 32 8684
PpuMI rG'GwC_Cy
Cuts at: 1142 5295 1142
Size: 4153 4563
PshAI GACnn'nnGTC
Cuts at: 850 850
Size: 8716
PspOMI G'GGCC_C
Cuts at: 2919 3408 4010 4204 4739 2919
Size: 489 602 194 535 6896
Fragments arranged by size:
6896 602 535 489 194
PstI C_TGCA'G
Cuts at: 3233 3977 4766 4832 4947 5012 5558 5579 5761
Size: 744 789 66 115 65 546 21 182
Cuts at: 5761 6446 3233
Size: 685 5503
Fragments arranged by size:
5503 789 744 685 546 182 115 66
65 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8716
PvuII CAG'CTG
Cuts at: 696 3507 3967 6139 6499 696
Size: 2811 460 2172 360 2913
Fragments arranged by size:
2913 2811 2172 460 360
RsrII CG'GwC_CG
Cuts at: 6909 6909
Size: 8716
SacI G_AGCT'C
Cuts at: 1346 1739 3527 4031 4364 5728 1346
Size: 393 1788 504 333 1364 4334
Fragments arranged by size:
4334 1788 1364 504 393 333
SalI G'TCGA_C
Cuts at: 5751 5751
Size: 8716
SanDI GG'GwC_CC
Cuts at: 1142 1142
Size: 8716
SapI GCTCTTCn'nnn_
Cuts at: 706 1351 3241 6737 6947 706
Size: 645 1890 3496 210 2475
Fragments arranged by size:
3496 2475 1890 645 210
SbfI CC_TGCA'GG
Cuts at: 5761 5761
Size: 8716
ScaI AGT'ACT
Cuts at: 8233 8233
Size: 8716
SexAI A'CCwGG_T
Cuts at: 858 3845 858
Size: 2987 5729
SfcI C'TryA_G
Cuts at: 33 992 1085 1451 2027 3229 3310 3517 3973
Size: 959 93 366 576 1202 81 207 456
Cuts at: 3973 4662 4762 4828 4943 5008 5554 5575 5757
Size: 689 100 66 115 65 546 21 182
Cuts at: 5757 6442 7437 7628 33
Size: 685 995 191 1121
Fragments arranged by size:
1202 1121 995 959 689 685 576 546
456 366 207 191 182 115 100 93
81 66 65 21
SfoI GGC'GCC
Cuts at: 4798 6146 6394 4798
Size: 1348 248 7120
Fragments arranged by size:
7120 1348 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8716
SmaI CCC'GGG
Cuts at: 1267 5736 1267
Size: 4469 4247
SmlI C'TyrA_G
Cuts at: 1438 1597 2962 4294 5315 5487 5869 6784 7278
Size: 159 1365 1332 1021 172 382 915 494
Cuts at: 7278 7540 1438
Size: 262 2614
Fragments arranged by size:
2614 1365 1332 1021 915 494 382 262
172 159
SnaBI TAC'GTA
Cuts at: 5706 5706
Size: 8716
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8716
SphI G_CATG'C
Cuts at: 921 1587 5767 6267 921
Size: 666 4180 500 3370
Fragments arranged by size:
4180 3370 666 500
SspI AAT'ATT
Cuts at: 141 141
Size: 8716
StuI AGG'CCT
Cuts at: 2054 2300 2054
Size: 246 8470
StyI C'CwwG_G
Cuts at: 125 1031 3257 4101 5306 5531 5561 5848 6202
Size: 906 2226 844 1205 225 30 287 354
Cuts at: 6202 125
Size: 2639
Fragments arranged by size:
2639 2226 1205 906 844 354 287 225
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 223 244 442 490 559 1942 2682 4942 6297
Size: 21 198 48 69 1383 740 2260 1355
Cuts at: 6297 6430 6889 7100 223
Size: 133 459 211 1839
Fragments arranged by size:
2260 1839 1383 1355 740 459 211 198
133 69 48 21
TatI w'GTAC_w
Cuts at: 1616 4845 5084 8231 1616
Size: 3229 239 3147 2101
Fragments arranged by size:
3229 3147 2101 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1480 1512 2372 2404 2501 2533 5083 5115 5335
Size: 32 860 32 97 32 2550 32 220
Cuts at: 5335 5367 5465 5497 8233 8265 1480
Size: 32 98 32 2736 32 1931
Fragments arranged by size:
2736 2550 1931 860 220 98 97 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6511 6511
Size: 8716
XbaI T'CTAG_A
Cuts at: 1050 5745 1050
Size: 4695 4021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 3853 4429 6209 741
Size: 3112 576 1780 3248
Fragments arranged by size:
3248 3112 1780 576
XmaI C'CCGG_G
Cuts at: 1265 5734 1265
Size: 4469 4247
XmnI GAAnn'nnTTC
Cuts at: 338 1813 4468 338
Size: 1475 2655 4586
Fragments arranged by size:
4586 2655 1475
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NruI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BssHII BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NotI NsiI PacI PasI PmlI PsiI
PspXI PsrI SacII SfiI SgrAI SrfI SwaI XhoI
ZraI