Restriction map for
pF1KSDA0252
(Circular) (Six-base) MAPSORT of: pF1KSDA0252.seq Check: 5415 from: 1 to: 5149
pF1KSDA0252 5149 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 298 298
Size: 5149
AccI GT'mk_AC
Cuts at: 938 2185 938
Size: 1247 3902
Acc65I G'GTAC_C
Cuts at: 2163 2163
Size: 5149
AclI AA'CG_TT
Cuts at: 4989 4989
Size: 5149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1576 2972 3404 4153 4421 1094
Size: 482 1396 432 749 268 1822
Fragments arranged by size:
1822 1396 749 482 432 268
AflIII A'CryG_T
Cuts at: 1308 2499 3605 4582 1308
Size: 1191 1106 977 1875
Fragments arranged by size:
1875 1191 1106 977
AgeI A'CCGG_T
Cuts at: 3537 3537
Size: 5149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1417 1417
Size: 5149
AlwNI CAG_nnn'CTG
Cuts at: 454 478 1554 2452 4021 454
Size: 24 1076 898 1569 1582
Fragments arranged by size:
1582 1569 1076 898 24
ApaI G_GGCC'C
Cuts at: 588 588
Size: 5149
ApaLI G'TGCA_C
Cuts at: 3919 3919
Size: 5149
ApoI r'AATT_y
Cuts at: 1342 1462 1944 2151 4502 4758 1342
Size: 120 482 207 2351 256 1733
Fragments arranged by size:
2351 1733 482 256 207 120
AseI AT'TA_AT
Cuts at: 20 20
Size: 5149
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
AvaI C'yCGr_G
Cuts at: 2167 2167
Size: 5149
BamHI G'GATC_C
Cuts at: 1779 2172 1779
Size: 393 4756
BanI G'GyrC_C
Cuts at: 1261 2163 2577 2825 2860 1261
Size: 902 414 248 35 3550
Fragments arranged by size:
3550 902 414 248 35
BanII G_rGCy'C
Cuts at: 209 448 588 902 2085 2161 3191 209
Size: 239 140 314 1183 76 1030 2167
Fragments arranged by size:
2167 1183 1030 314 239 140 76
BbeI G_GCGC'C
Cuts at: 2581 2829 2581
Size: 248 4901
BbsI GAAGACnn'nnnn_
Cuts at: 563 584 1549 2114 563
Size: 21 965 565 3598
Fragments arranged by size:
3598 965 565 21
BbvCI CC'TCA_GC
Cuts at: 1574 1574
Size: 5149
BciVI GTATCCnnnnn_n'
Cuts at: 3045 3808 5149 3045
Size: 763 1341 3045
Fragments arranged by size:
3045 1341 763
BclI T'GATC_A
Cuts at: 1470 2667 1470
Size: 1197 3952
BglI GCCn_nnn'nGGC
Cuts at: 1744 1855 1744
Size: 111 5038
BglII A'GATC_T
Cuts at: 1974 2092 2662 4701 1974
Size: 118 570 2039 2422
Fragments arranged by size:
2422 2039 570 118
BlpI GC'TnA_GC
Cuts at: 447 896 2259 447
Size: 449 1363 3337
Fragments arranged by size:
3337 1363 449
Bme1580I G_kGCm'C
Cuts at: 588 2772 2865 3923 588
Size: 2184 93 1058 1814
Fragments arranged by size:
2184 1814 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 2359 2440 2775 4921 2359
Size: 81 335 2146 2587
Fragments arranged by size:
2587 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 957 1347 3514 577
Size: 380 390 2167 2212
Fragments arranged by size:
2212 2167 390 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 1904 1651
Size: 253 4896
Bpu10I CC'TnA_GC
Cuts at: 1574 1574
Size: 5149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 2323 3202 3696 3994 827
Size: 1496 879 494 298 1982
Fragments arranged by size:
1982 1496 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1823 4853 1823
Size: 3030 2119
BsaAI yAC'GTr
Cuts at: 2139 2500 2139
Size: 361 4788
BsaBI GATnn'nnATC
Cuts at: 6 1174 2661 4700 6
Size: 1168 1487 2039 455
Fragments arranged by size:
2039 1487 1168 455
BsaHI Gr'CG_yC
Cuts at: 295 2578 2826 3530 5026 295
Size: 2283 248 704 1496 418
Fragments arranged by size:
2283 1496 704 418 248
BsaWI w'CCGG_w
Cuts at: 2336 2857 3537 3811 3958 4369 2336
Size: 521 680 274 147 411 3116
Fragments arranged by size:
3116 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 196 226 196
Size: 30 5119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 399 483 754 868 970 1042 259
Size: 140 84 271 114 102 72 4366
Fragments arranged by size:
4366 271 140 114 102 84 72
BseYI C'CCAG_C
Cuts at: 1833 1859 2023 2572 3330 3909 4405 1833
Size: 26 164 549 758 579 496 2577
Fragments arranged by size:
2577 758 579 549 496 164 26
BsiEI CG_ry'CG
Cuts at: 104 2689 2735 3945 104
Size: 2585 46 1210 1308
Fragments arranged by size:
2585 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 209 448 902 2161 2939 3129 3923 209
Size: 239 454 1259 778 190 794 1435
Fragments arranged by size:
1435 1259 794 778 454 239 190
BsmI GAATG_Cn'
Cuts at: 1872 1872
Size: 5149
BsmBI CGTCTCn'nnnn_
Cuts at: 286 286
Size: 5149
Bsp1286I G_dGCh'C
Cuts at: 209 448 588 902 2085 2161 2772 2865 2939
Size: 239 140 314 1183 76 611 93 74
Cuts at: 2939 3129 3191 3923 209
Size: 190 62 732 1435
Fragments arranged by size:
1435 1183 732 611 314 239 190 140
93 76 74 62
BspEI T'CCGG_A
Cuts at: 4369 4369
Size: 5149
BspHI T'CATG_A
Cuts at: 4325 4325
Size: 5149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2197 2411 2713 3094 2197
Size: 214 302 381 4252
Fragments arranged by size:
4252 381 302 214
BsrBI CCG'CTC
Cuts at: 50 995 3439 3493 4979 5144 50
Size: 945 2444 54 1486 165 55
Fragments arranged by size:
2444 1486 945 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1305 3059 1305
Size: 1754 3395
BsrFI r'CCGG_y
Cuts at: 3145 3326 3537 3145
Size: 181 211 4757
Fragments arranged by size:
4757 211 181
BssSI C'ACGA_G
Cuts at: 3418 3778 3418
Size: 360 4789
BstAPI GCAn_nnn'nTGC
Cuts at: 2524 2524
Size: 5149
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5149
BstXI CCAn_nnnn'nTGG
Cuts at: 1258 1258
Size: 5149
BstYI r'GATC_y
Cuts at: 1 1040 1779 1974 2092 2172 2639 2662 2997
Size: 1039 739 195 118 80 467 23 335
Cuts at: 2997 3243 4257 4343 4355 4701 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1039 1014 739 467 449 346 335 246
195 118 86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 411 455 411
Size: 44 5105
BtgI C'CryG_G
Cuts at: 125 848 1568 125
Size: 723 720 3706
Fragments arranged by size:
3706 723 720
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1393 3097 3278 3441 1393
Size: 1704 181 163 3101
Fragments arranged by size:
3101 1704 181 163
BtsI GCAGTG_nn'
Cuts at: 240 1804 2390 240
Size: 1564 586 2999
Fragments arranged by size:
2999 1564 586
DraI TTT'AAA
Cuts at: 2146 2146
Size: 5149
DrdI GACnn_nn'nnGTC
Cuts at: 2853 3713 2853
Size: 860 4289
EaeI y'GGCC_r
Cuts at: 2732 2906 3297 3324 4671 2732
Size: 174 391 27 1347 3210
Fragments arranged by size:
3210 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2732 2732
Size: 5149
EarI CTCTTCn'nnn_
Cuts at: 501 522 527 640 731 947 956 1019 1031
Size: 21 5 113 91 216 9 63 12
Cuts at: 1031 1478 3170 3380 501
Size: 447 1692 210 2270
Fragments arranged by size:
2270 1692 447 216 210 113 91 63
21 12 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2054 3355 3667 3813 4959 2054
Size: 1301 312 146 1146 2244
Fragments arranged by size:
2244 1301 1146 312 146
EcoICRI GAG'CTC
Cuts at: 207 446 900 2159 207
Size: 239 454 1259 3197
Fragments arranged by size:
3197 1259 454 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1094 1576 1651 1904 2972 3404 4153 4421 1094
Size: 482 75 253 1068 432 749 268 1822
Fragments arranged by size:
1822 1068 749 482 432 268 253 75
EcoNI CCTnn'n_nnAGG
Cuts at: 199 458 199
Size: 259 4890
EcoO109I rG'GnC_Cy
Cuts at: 584 1578 1647 1737 2286 584
Size: 994 69 90 549 3447
Fragments arranged by size:
3447 994 549 90 69
EcoRI G'AATT_C
Cuts at: 1342 1944 2151 1342
Size: 602 207 4340
Fragments arranged by size:
4340 602 207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1468 2028 3166 1468
Size: 560 1138 3451
Fragments arranged by size:
3451 1138 560
FspI TGC'GCA
Cuts at: 2928 2928
Size: 5149
HaeII r_GCGC'y
Cuts at: 2581 2829 3853 4832 2581
Size: 248 1024 979 2898
Fragments arranged by size:
2898 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 499 505 531 537 659 691 823
Size: 32 271 6 26 6 122 32 132
Cuts at: 823 855 958 982 990 1014 1905 1937 2989
Size: 32 103 24 8 24 891 32 1052
Cuts at: 2989 3019 3021 3051 196
Size: 30 2 30 2294
Fragments arranged by size:
2294 1052 891 271 132 122 103 32
32 32 32 30 30 26 24 24
8 6 6 2
HincII GTy'rAC
Cuts at: 2186 2186
Size: 5149
KasI G'GCGC_C
Cuts at: 2577 2825 2577
Size: 248 4901
KpnI G_GTAC'C
Cuts at: 2167 2167
Size: 5149
MluI A'CGCG_T
Cuts at: 1308 4582 1308
Size: 3274 1875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4004 3820
Size: 184 4965
MscI TGG'CCA
Cuts at: 2908 2908
Size: 5149
MslI CAynn'nnrTG
Cuts at: 1298 3136 3263 1298
Size: 1838 127 3184
Fragments arranged by size:
3184 1838 127
MspA1I CmG'CkG
Cuts at: 179 439 895 1662 1701 1837 1863 2258 2572
Size: 260 456 767 39 136 26 395 314
Cuts at: 2572 2932 3947 4192 179
Size: 360 1015 245 1136
Fragments arranged by size:
1136 1015 767 456 395 360 314 260
245 136 39 26
NaeI GCC'GGC
Cuts at: 3328 3328
Size: 5149
NarI GG'CG_CC
Cuts at: 2578 2826 2578
Size: 248 4901
NcoI C'CATG_G
Cuts at: 125 848 1568 125
Size: 723 720 3706
Fragments arranged by size:
3706 723 720
NgoMIV G'CCGG_C
Cuts at: 3326 3326
Size: 5149
NruI TCG'CGA
Cuts at: 3510 3510
Size: 5149
NspI r_CATG'y
Cuts at: 1131 1398 2200 2700 3609 1131
Size: 267 802 500 909 2671
Fragments arranged by size:
2671 909 802 500 267
PciI A'CATG_T
Cuts at: 3605 3605
Size: 5149
PflMI CCAn_nnn'nTGG
Cuts at: 1776 1776
Size: 5149
PmeI GTTT'AAAC
Cuts at: 2146 2146
Size: 5149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4320 4288
Size: 32 5117
PpuMI rG'GwC_Cy
Cuts at: 1578 1578
Size: 5149
PsiI TTA'TAA
Cuts at: 1460 1460
Size: 5149
PspOMI G'GGCC_C
Cuts at: 584 584
Size: 5149
PstI C_TGCA'G
Cuts at: 276 2194 2879 276
Size: 1918 685 2546
Fragments arranged by size:
2546 1918 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5149
PvuII CAG'CTG
Cuts at: 439 895 1837 1863 2572 2932 439
Size: 456 942 26 709 360 2656
Fragments arranged by size:
2656 942 709 456 360 26
RsrII CG'GwC_CG
Cuts at: 3342 3342
Size: 5149
SacI G_AGCT'C
Cuts at: 209 448 902 2161 209
Size: 239 454 1259 3197
Fragments arranged by size:
3197 1259 454 239
SalI G'TCGA_C
Cuts at: 2184 2184
Size: 5149
SapI GCTCTTCn'nnn_
Cuts at: 3170 3380 3170
Size: 210 4939
SbfI CC_TGCA'GG
Cuts at: 2194 2194
Size: 5149
ScaI AGT'ACT
Cuts at: 4666 4666
Size: 5149
SfcI C'TryA_G
Cuts at: 33 272 2190 2875 3870 4061 33
Size: 239 1918 685 995 191 1121
Fragments arranged by size:
1918 1121 995 685 239 191
SfoI GGC'GCC
Cuts at: 2579 2827 2579
Size: 248 4901
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5149
SmaI CCC'GGG
Cuts at: 2169 2169
Size: 5149
SmlI C'TyrA_G
Cuts at: 842 2302 3217 3711 3973 842
Size: 1460 915 494 262 2018
Fragments arranged by size:
2018 1460 915 494 262
SnaBI TAC'GTA
Cuts at: 2139 2139
Size: 5149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5149
SphI G_CATG'C
Cuts at: 1398 2200 2700 1398
Size: 802 500 3847
Fragments arranged by size:
3847 802 500
StyI C'CwwG_G
Cuts at: 125 848 1568 2057 2072 2086 2281 2635 125
Size: 723 720 489 15 14 195 354 2639
Fragments arranged by size:
2639 723 720 489 354 195 15 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1539 2730 2863 3322 3533 1539
Size: 1191 133 459 211 3155
Fragments arranged by size:
3155 1191 459 211 133
TatI w'GTAC_w
Cuts at: 1986 4664 1986
Size: 2678 2471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1148 1180 4666 4698 1148
Size: 32 3486 32 1599
Fragments arranged by size:
3486 1599 32 32
Tth111I GACn'n_nGTC
Cuts at: 2944 2944
Size: 5149
XbaI T'CTAG_A
Cuts at: 1410 2178 1410
Size: 768 4381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 547 2642 547
Size: 2095 3054
XmaI C'CCGG_G
Cuts at: 2167 2167
Size: 5149
ZraI GAC'GTC
Cuts at: 296 296
Size: 5149
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI