Restriction map for
pF1KSDA0253
(Circular) (Six-base) MAPSORT of: pF1KSDA0253.seq Check: 5016 from: 1 to: 5266
pF1KSDA0253 5266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 820 820
Size: 5266
AccI GT'mk_AC
Cuts at: 1157 2302 1157
Size: 1145 4121
Acc65I G'GTAC_C
Cuts at: 2280 2280
Size: 5266
AclI AA'CG_TT
Cuts at: 5106 5106
Size: 5266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1073 1565 1629 3089 3521 4270 4538 828
Size: 245 492 64 1460 432 749 268 1556
Fragments arranged by size:
1556 1460 749 492 432 268 245 64
AflII C'TTAA_G
Cuts at: 1233 1233
Size: 5266
AflIII A'CryG_T
Cuts at: 2616 3722 4699 2616
Size: 1106 977 3183
Fragments arranged by size:
3183 1106 977
AgeI A'CCGG_T
Cuts at: 3654 3654
Size: 5266
AhdI GACnn_n'nnGTC
Cuts at: 876 1752 876
Size: 876 4390
AleI CACnn'nnGTG
Cuts at: 1460 1460
Size: 5266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 231
Size: 5266
AlwNI CAG_nnn'CTG
Cuts at: 1009 1270 1573 2242 2569 4138 1009
Size: 261 303 669 327 1569 2137
Fragments arranged by size:
2137 1569 669 327 303 261
ApaI G_GGCC'C
Cuts at: 1948 1948
Size: 5266
ApaLI G'TGCA_C
Cuts at: 4036 4036
Size: 5266
ApoI r'AATT_y
Cuts at: 618 1842 2268 4619 4875 618
Size: 1224 426 2351 256 1009
Fragments arranged by size:
2351 1224 1009 426 256
AseI AT'TA_AT
Cuts at: 20 1514 20
Size: 1494 3772
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
AvaI C'yCGr_G
Cuts at: 159 1146 1871 2228 2284 159
Size: 987 725 357 56 3141
Fragments arranged by size:
3141 987 725 357 56
BamHI G'GATC_C
Cuts at: 1887 2289 1887
Size: 402 4864
BanI G'GyrC_C
Cuts at: 1068 1462 2280 2694 2942 2977 1068
Size: 394 818 414 248 35 3357
Fragments arranged by size:
3357 818 414 394 248 35
BanII G_rGCy'C
Cuts at: 1425 1543 1948 2050 2278 3308 1425
Size: 118 405 102 228 1030 3383
Fragments arranged by size:
3383 1030 405 228 118 102
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5018
BbsI GAAGACnn'nnnn_
Cuts at: 308 653 1101 308
Size: 345 448 4473
Fragments arranged by size:
4473 448 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1862 1896 1862
Size: 34 5232
BciVI GTATCCnnnnn_n'
Cuts at: 3162 3925 5266 3162
Size: 763 1341 3162
Fragments arranged by size:
3162 1341 763
BclI T'GATC_A
Cuts at: 2135 2784 2135
Size: 649 4617
BfrBI ATG'CAT
Cuts at: 789 789
Size: 5266
BglI GCCn_nnn'nGGC
Cuts at: 1006 1006
Size: 5266
BglII A'GATC_T
Cuts at: 2779 4818 2779
Size: 2039 3227
BlpI GC'TnA_GC
Cuts at: 741 1537 2376 741
Size: 796 839 3631
Fragments arranged by size:
3631 839 796
Bme1580I G_kGCm'C
Cuts at: 547 1948 2100 2889 2982 4040 547
Size: 1401 152 789 93 1058 1773
Fragments arranged by size:
1773 1401 1058 789 152 93
BmgBI CAC'GTC
Cuts at: 1612 1612
Size: 5266
BmrI ACTGGGnnnn_n'
Cuts at: 503 932 2476 2557 2892 5038 503
Size: 429 1544 81 335 2146 731
Fragments arranged by size:
2146 1544 731 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3631 3631
Size: 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1782 439
Size: 1343 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 2440 3319 3813 4111 2149
Size: 291 879 494 298 3304
Fragments arranged by size:
3304 879 494 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 178 4970 178
Size: 4792 474
BsaAI yAC'GTr
Cuts at: 348 2256 2617 348
Size: 1908 361 2997
Fragments arranged by size:
2997 1908 361
BsaBI GATnn'nnATC
Cuts at: 6 247 2778 4817 6
Size: 241 2531 2039 455
Fragments arranged by size:
2531 2039 455 241
BsaHI Gr'CG_yC
Cuts at: 2695 2943 3647 5143 2695
Size: 248 704 1496 2818
Fragments arranged by size:
2818 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2453 2974 3654 3928 4075 4486 2453
Size: 521 680 274 147 411 3233
Fragments arranged by size:
3233 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 376 376
Size: 5266
BseYI C'CCAG_C
Cuts at: 624 1037 1218 1382 1605 2689 3447 4026 4522
Size: 413 181 164 223 1084 758 579 496
Cuts at: 4522 624
Size: 1368
Fragments arranged by size:
1368 1084 758 579 496 413 223 181
164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1207 1982 237
Size: 970 775 3521
Fragments arranged by size:
3521 970 775
BsiEI CG_ry'CG
Cuts at: 104 2806 2852 4062 104
Size: 2702 46 1210 1308
Fragments arranged by size:
2702 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1425 1617 2050 2278 3056 3246 4040 1425
Size: 192 433 228 778 190 794 2651
Fragments arranged by size:
2651 794 778 433 228 192 190
BsmI GAATG_Cn'
Cuts at: 1953 1953
Size: 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 1957 1957
Size: 5266
Bsp1286I G_dGCh'C
Cuts at: 547 1425 1543 1617 1948 2050 2100 2278 2889
Size: 878 118 74 331 102 50 178 611
Cuts at: 2889 2982 3056 3246 3308 4040 547
Size: 93 74 190 62 732 1773
Fragments arranged by size:
1773 878 732 611 331 190 178 118
102 93 74 74 62 50
BspEI T'CCGG_A
Cuts at: 4486 4486
Size: 5266
BspHI T'CATG_A
Cuts at: 4442 4442
Size: 5266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 199 820 2314 2528 2830 3211 199
Size: 621 1494 214 302 381 2254
Fragments arranged by size:
2254 1494 621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3556 3610 5096 5261 50
Size: 3506 54 1486 165 55
Fragments arranged by size:
3506 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 636 1101 2219 3176 636
Size: 465 1118 957 2726
Fragments arranged by size:
2726 1118 957 465
BsrFI r'CCGG_y
Cuts at: 3262 3443 3654 3262
Size: 181 211 4874
Fragments arranged by size:
4874 211 181
BsrGI T'GTAC_A
Cuts at: 537 1824 537
Size: 1287 3979
BssSI C'ACGA_G
Cuts at: 3535 3895 3535
Size: 360 4906
BstAPI GCAn_nnn'nTGC
Cuts at: 2641 2641
Size: 5266
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5266
BstEII G'GTnAC_C
Cuts at: 1677 1677
Size: 5266
BstXI CCAn_nnnn'nTGG
Cuts at: 969 1584 1605 969
Size: 615 21 4630
Fragments arranged by size:
4630 615 21
BstYI r'GATC_y
Cuts at: 1 1266 1311 1887 1917 2289 2756 2779 3114
Size: 1265 45 576 30 372 467 23 335
Cuts at: 3114 3360 4374 4460 4472 4818 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1265 1014 576 467 449 372 346 335
246 86 45 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 519 1363 1738 1750 519
Size: 844 375 12 4035
Fragments arranged by size:
4035 844 375 12
BtgI C'CryG_G
Cuts at: 125 1598 125
Size: 1473 3793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1773 2097 3214 3395 3558 1773
Size: 324 1117 181 163 3481
Fragments arranged by size:
3481 1117 324 181 163
BtsI GCAGTG_nn'
Cuts at: 497 597 712 803 1025 1499 1571 1885 2507
Size: 100 115 91 222 474 72 314 622
Cuts at: 2507 497
Size: 3256
Fragments arranged by size:
3256 622 474 314 222 115 100 91
72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1803 1838 1803
Size: 35 5231
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5266
DraIII CAC_nnn'GTG
Cuts at: 763 763
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 2970 3830 2970
Size: 860 4406
EaeI y'GGCC_r
Cuts at: 1319 1595 2849 3023 3414 3441 4788 1319
Size: 276 1254 174 391 27 1347 1797
Fragments arranged by size:
1797 1347 1254 391 276 174 27
EagI C'GGCC_G
Cuts at: 2849 2849
Size: 5266
EarI CTCTTCn'nnn_
Cuts at: 1327 1541 1781 3287 3497 1327
Size: 214 240 1506 210 3096
Fragments arranged by size:
3096 1506 240 214 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3472 3784 3930 5076 3472
Size: 312 146 1146 3662
Fragments arranged by size:
3662 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1423 2048 2276 1423
Size: 625 228 4413
Fragments arranged by size:
4413 625 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 828 1073 1565 1629 1782 3089 3521 4270
Size: 389 245 492 64 153 1307 432 749
Cuts at: 4270 4538 439
Size: 268 1167
Fragments arranged by size:
1307 1167 749 492 432 389 268 245
153 64
EcoNI CCTnn'n_nnAGG
Cuts at: 518 1741 518
Size: 1223 4043
EcoO109I rG'GnC_Cy
Cuts at: 366 1551 1581 1746 1754 1944 1945 2012 2403
Size: 1185 30 165 8 190 1 67 391
Cuts at: 2403 366
Size: 3229
Fragments arranged by size:
3229 1185 391 190 165 67 30 8
1
EcoRI G'AATT_C
Cuts at: 618 2268 618
Size: 1650 3616
EcoRV GAT'ATC
Cuts at: 2091 2091
Size: 5266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 5266
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5266
HaeII r_GCGC'y
Cuts at: 2698 2946 3970 4949 2698
Size: 248 1024 979 3015
Fragments arranged by size:
3015 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 1359 1391 1738 1770 3106 3136 3138
Size: 32 465 32 347 32 1336 30 2
Cuts at: 3138 3168 862
Size: 30 2960
Fragments arranged by size:
2960 1336 465 347 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1203 1863 2303 1203
Size: 660 440 4166
Fragments arranged by size:
4166 660 440
HindIII A'AGCT_T
Cuts at: 1055 1055
Size: 5266
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5018
KpnI G_GTAC'C
Cuts at: 2284 2284
Size: 5266
MluI A'CGCG_T
Cuts at: 4699 4699
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4121 3937
Size: 184 5082
MscI TGG'CCA
Cuts at: 1321 1597 3025 1321
Size: 276 1428 3562
Fragments arranged by size:
3562 1428 276
MslI CAynn'nnrTG
Cuts at: 788 814 1460 1516 1958 3253 3380 788
Size: 26 646 56 442 1295 127 2674
Fragments arranged by size:
2674 1295 646 442 127 56 26
MspA1I CmG'CkG
Cuts at: 1041 2081 2375 2689 3049 4064 4309 1041
Size: 1040 294 314 360 1015 245 1998
Fragments arranged by size:
1998 1040 1015 360 314 294 245
NaeI GCC'GGC
Cuts at: 3445 3445
Size: 5266
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5018
NcoI C'CATG_G
Cuts at: 125 125
Size: 5266
NgoMIV G'CCGG_C
Cuts at: 3443 3443
Size: 5266
NruI TCG'CGA
Cuts at: 3627 3627
Size: 5266
NsiI A_TGCA'T
Cuts at: 791 791
Size: 5266
NspI r_CATG'y
Cuts at: 789 793 819 903 2317 2817 3726 789
Size: 4 26 84 1414 500 909 2329
Fragments arranged by size:
2329 1414 909 500 84 26 4
PasI CC'CwG_GG
Cuts at: 1003 1003
Size: 5266
PciI A'CATG_T
Cuts at: 3722 3722
Size: 5266
PflMI CCAn_nnn'nTGG
Cuts at: 553 553
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4405 4437 4405
Size: 32 5234
PpuMI rG'GwC_Cy
Cuts at: 366 1551 1746 1754 366
Size: 1185 195 8 3878
Fragments arranged by size:
3878 1185 195 8
PspOMI G'GGCC_C
Cuts at: 1944 1944
Size: 5266
PspXI vC'TCGA_Gb
Cuts at: 1146 1146
Size: 5266
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 5234
PstI C_TGCA'G
Cuts at: 604 1409 2311 2996 604
Size: 805 902 685 2874
Fragments arranged by size:
2874 902 805 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5266
PvuII CAG'CTG
Cuts at: 1041 2689 3049 1041
Size: 1648 360 3258
Fragments arranged by size:
3258 1648 360
RsrII CG'GwC_CG
Cuts at: 1968 3459 1968
Size: 1491 3775
SacI G_AGCT'C
Cuts at: 1425 2050 2278 1425
Size: 625 228 4413
Fragments arranged by size:
4413 625 228
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5266
SapI GCTCTTCn'nnn_
Cuts at: 3287 3497 3287
Size: 210 5056
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5266
ScaI AGT'ACT
Cuts at: 1930 4783 1930
Size: 2853 2413
SexAI A'CCwGG_T
Cuts at: 1301 1301
Size: 5266
SfcI C'TryA_G
Cuts at: 33 370 600 1405 2307 2992 3987 4178 33
Size: 337 230 805 902 685 995 191 1121
Fragments arranged by size:
1121 995 902 805 685 337 230 191
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5018
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
SmaI CCC'GGG
Cuts at: 161 2230 2286 161
Size: 2069 56 3141
Fragments arranged by size:
3141 2069 56
SmlI C'TyrA_G
Cuts at: 1146 1233 2128 2419 3334 3828 4090 1146
Size: 87 895 291 915 494 262 2322
Fragments arranged by size:
2322 915 895 494 291 262 87
SnaBI TAC'GTA
Cuts at: 2256 2256
Size: 5266
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5266
SphI G_CATG'C
Cuts at: 793 819 903 2317 2817 793
Size: 26 84 1414 500 3242
Fragments arranged by size:
3242 1414 500 84 26
SspI AAT'ATT
Cuts at: 1480 1480
Size: 5266
StyI C'CwwG_G
Cuts at: 125 1577 1835 2398 2752 125
Size: 1452 258 563 354 2639
Fragments arranged by size:
2639 1452 563 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1051 1753 1985 2847 2980 3439 3650 1051
Size: 702 232 862 133 459 211 2667
Fragments arranged by size:
2667 862 702 459 232 211 133
TatI w'GTAC_w
Cuts at: 537 1824 1928 4781 537
Size: 1287 104 2853 1022
Fragments arranged by size:
2853 1287 1022 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 358 390 737 769 4783 4815 215
Size: 32 111 32 347 32 4014 32 666
Fragments arranged by size:
4014 666 347 111 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5266
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 2759 444
Size: 2315 2951
XhoI C'TCGA_G
Cuts at: 1146 1146
Size: 5266
XmaI C'CCGG_G
Cuts at: 159 2228 2284 159
Size: 2069 56 3141
Fragments arranged by size:
3141 2069 56
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AloI AscI AvrII BaeI BbvCI BmtI
Bpu10I BsaXI BsiWI BssHII BstZ17I ClaI FseI FspAI
HpaI MfeI NdeI NheI NotI PacI PfoI PmlI
PshAI PsiI SacII SanDI SfiI SgrAI SrfI StuI
SwaI XmnI ZraI