Restriction map for
pF1KSDA0258
(Circular) (Six-base) MAPSORT of: pF1KSDA0258.seq Check: 4829 from: 1 to: 4310
pF1KSDA0258 4310 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 530 1199 1348 530
Size: 669 149 3492
Fragments arranged by size:
3492 669 149
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1191 2135 2567 3314 3582 601
Size: 590 944 432 747 268 1329
Fragments arranged by size:
1329 944 747 590 432 268
AflIII A'CryG_T
Cuts at: 972 1662 2766 3743 972
Size: 690 1104 977 1539
Fragments arranged by size:
1539 1104 977 690
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AleI CACnn'nnGTG
Cuts at: 377 377
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1183 1615 3182 1183
Size: 432 1567 2311
Fragments arranged by size:
2311 1567 432
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 416 1105 1314 3663 3919 416
Size: 689 209 2349 256 807
Fragments arranged by size:
2349 807 689 256 209
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 1118 1330 1118
Size: 212 4098
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 989 1326 1740 1988 2023 989
Size: 337 414 248 35 3276
Fragments arranged by size:
3276 414 337 248 35
BanII G_rGCy'C
Cuts at: 149 1324 2354 149
Size: 1175 1030 2105
Fragments arranged by size:
2105 1175 1030
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BciVI GTATCCnnnnn_n'
Cuts at: 406 2208 2969 4310 406
Size: 1802 761 1341 406
Fragments arranged by size:
1802 1341 761 406
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 148 710 1422 148
Size: 562 712 3036
Fragments arranged by size:
3036 712 562
Bme1580I G_kGCm'C
Cuts at: 267 1935 2028 3084 267
Size: 1668 93 1056 1493
Fragments arranged by size:
1668 1493 1056 93
BmgBI CAC'GTC
Cuts at: 840 943 840
Size: 103 4207
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BmtI G_CTAG'C
Cuts at: 709 709
Size: 4310
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 467 205
Size: 262 4048
Bpu10I CC'TnA_GC
Cuts at: 1184 1184
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2365 2857 3155 1486
Size: 879 492 298 2641
Fragments arranged by size:
2641 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 1302 1663 1302
Size: 361 3949
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 626 1499 2020 2698 2972 3119 3530 626
Size: 873 521 678 274 147 411 1406
Fragments arranged by size:
1406 873 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 305 688 925 305
Size: 383 237 3690
Fragments arranged by size:
3690 383 237
BseYI C'CCAG_C
Cuts at: 172 718 1211 1276 1735 2493 3070 3566 172
Size: 546 493 65 459 758 577 496 916
Fragments arranged by size:
916 758 577 546 496 493 459 65
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 1324 2102 2292 3084 149
Size: 1175 778 190 792 1375
Fragments arranged by size:
1375 1175 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 149 267 1324 1935 2028 2102 2292 2354 3084
Size: 118 1057 611 93 74 190 62 730
Cuts at: 3084 149
Size: 1375
Fragments arranged by size:
1375 1057 730 611 190 118 93 74
62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1226 1360 1574 1876 2257 1226
Size: 134 214 302 381 3279
Fragments arranged by size:
3279 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BsrGI T'GTAC_A
Cuts at: 821 918 1066 821
Size: 97 148 4065
Fragments arranged by size:
4065 148 97
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 110 2671 110
Size: 2561 1749
BstYI r'GATC_y
Cuts at: 1 1335 1802 1825 2160 2406 3418 3504 3516
Size: 1334 467 23 335 246 1012 86 12
Cuts at: 3516 3862 1
Size: 346 449
Fragments arranged by size:
1334 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 433 433
Size: 4310
BtgI C'CryG_G
Cuts at: 221 221
Size: 4310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 273 307 1553 273
Size: 34 1246 3030
Fragments arranged by size:
3030 1246 34
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 224 780 1895 2069 2460 2487 3832 224
Size: 556 1115 174 391 27 1345 702
Fragments arranged by size:
1345 1115 702 556 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 206 2518 2828 2974 4120 206
Size: 2312 310 146 1146 396
Fragments arranged by size:
2312 1146 396 310 146
EcoICRI GAG'CTC
Cuts at: 147 1322 147
Size: 1175 3135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 467 601 1191 2135 2567 3314 3582 205
Size: 262 134 590 944 432 747 268 933
Fragments arranged by size:
944 933 747 590 432 268 262 134
EcoO109I rG'GnC_Cy
Cuts at: 157 246 848 1180 1215 1273 1449 157
Size: 89 602 332 35 58 176 3018
Fragments arranged by size:
3018 602 332 176 89 58 35
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 184 1744 1992 3014 3993 184
Size: 1560 248 1022 979 501
Fragments arranged by size:
1560 1022 979 501 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 442 458 474 1121 1153 2152 2182 2184
Size: 16 16 16 647 32 999 30 2
Cuts at: 2184 2214 426
Size: 30 2522
Fragments arranged by size:
2522 999 647 32 30 30 16 16
16 2
HincII GTy'rAC
Cuts at: 561 1349 561
Size: 788 3522
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 290 377 1228 2299 2426 290
Size: 87 851 1071 127 2174
Fragments arranged by size:
2174 1071 851 127 87
MspA1I CmG'CkG
Cuts at: 936 1211 1421 1735 2095 3108 3353 936
Size: 275 210 314 360 1013 245 1893
Fragments arranged by size:
1893 1013 360 314 275 245 210
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NdeI CA'TA_TG
Cuts at: 1260 1260
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NheI G'CTAG_C
Cuts at: 705 705
Size: 4310
NspI r_CATG'y
Cuts at: 976 1363 1863 2770 976
Size: 387 500 907 2516
Fragments arranged by size:
2516 907 500 387
PciI A'CATG_T
Cuts at: 972 2766 972
Size: 1794 2516
PfoI T'CCnGG_A
Cuts at: 444 444
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 157 848 1180 1215 157
Size: 691 332 35 3252
Fragments arranged by size:
3252 691 332 35
PshAI GACnn'nnGTC
Cuts at: 326 326
Size: 4310
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1211 1735 2095 1211
Size: 524 360 3426
Fragments arranged by size:
3426 524 360
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 149 1324 149
Size: 1175 3135
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 468 647 1196 1353 2038 3031 3222 33
Size: 435 179 549 157 685 993 191 1121
Fragments arranged by size:
1121 993 685 549 435 191 179 157
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1465 2380 2872 3134 1465
Size: 915 492 262 2641
Fragments arranged by size:
2641 915 492 262
SnaBI TAC'GTA
Cuts at: 1302 1302
Size: 4310
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
StyI C'CwwG_G
Cuts at: 1444 1798 1444
Size: 354 3956
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 922 1893 2026 2485 2694 922
Size: 971 133 459 209 2538
Fragments arranged by size:
2538 971 459 209 133
TatI w'GTAC_w
Cuts at: 821 918 1066 3825 821
Size: 97 148 2759 1306
Fragments arranged by size:
2759 1306 148 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 982 1014 3827 3859 982
Size: 32 2813 32 1433
Fragments arranged by size:
2813 1433 32 32
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 246 666 940 1805 132
Size: 114 420 274 865 2637
Fragments arranged by size:
2637 865 420 274 114
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI