Restriction map for
pF1KSDA0262
(Circular) (Six-base) MAPSORT of: pF1KSDA0262.seq Check: 5606 from: 1 to: 5572
pF1KSDA0262 5572 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1131 1131
Size: 5572
AccI GT'mk_AC
Cuts at: 1070 2608 1070
Size: 1538 4034
Acc65I G'GTAC_C
Cuts at: 2586 2586
Size: 5572
AclI AA'CG_TT
Cuts at: 5412 5412
Size: 5572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 2403 2453 2504 3395 3827 4576 4844 719
Size: 1684 50 51 891 432 749 268 1447
Fragments arranged by size:
1684 1447 891 749 432 268 51 50
AflIII A'CryG_T
Cuts at: 2922 4028 5005 2922
Size: 1106 977 3489
Fragments arranged by size:
3489 1106 977
AgeI A'CCGG_T
Cuts at: 3960 3960
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2069 2069
Size: 5572
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 5540
AlwNI CAG_nnn'CTG
Cuts at: 1146 1191 1492 1504 1756 1850 2271 2875 4444
Size: 45 301 12 252 94 421 604 1569
Cuts at: 4444 1146
Size: 2274
Fragments arranged by size:
2274 1569 604 421 301 252 94 45
12
ApaI G_GGCC'C
Cuts at: 2124 2124
Size: 5572
ApaLI G'TGCA_C
Cuts at: 4342 4342
Size: 5572
ApoI r'AATT_y
Cuts at: 558 2412 2574 4925 5181 558
Size: 1854 162 2351 256 949
Fragments arranged by size:
2351 1854 949 256 162
AseI AT'TA_AT
Cuts at: 20 20
Size: 5572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5572
AvaI C'yCGr_G
Cuts at: 1213 1256 1738 1949 2032 2590 1213
Size: 43 482 211 83 558 4195
Fragments arranged by size:
4195 558 482 211 83 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 728 761 728
Size: 33 5539
BamHI G'GATC_C
Cuts at: 301 2595 301
Size: 2294 3278
BanI G'GyrC_C
Cuts at: 2586 3000 3248 3283 2586
Size: 414 248 35 4875
Fragments arranged by size:
4875 414 248 35
BanII G_rGCy'C
Cuts at: 139 1250 1738 2124 2584 3614 139
Size: 1111 488 386 460 1030 2097
Fragments arranged by size:
2097 1111 1030 488 460 386
BbeI G_GCGC'C
Cuts at: 3004 3252 3004
Size: 248 5324
BbsI GAAGACnn'nnnn_
Cuts at: 474 707 904 2246 474
Size: 233 197 1342 3800
Fragments arranged by size:
3800 1342 233 197
BbvCI CC'TCA_GC
Cuts at: 1624 2272 1624
Size: 648 4924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2526 2560 2526
Size: 34 5538
BciVI GTATCCnnnnn_n'
Cuts at: 3468 4231 5572 3468
Size: 763 1341 3468
Fragments arranged by size:
3468 1341 763
BclI T'GATC_A
Cuts at: 3090 3090
Size: 5572
BfrBI ATG'CAT
Cuts at: 869 869
Size: 5572
BglII A'GATC_T
Cuts at: 1745 3085 5124 1745
Size: 1340 2039 2193
Fragments arranged by size:
2193 2039 1340
BlpI GC'TnA_GC
Cuts at: 133 2682 133
Size: 2549 3023
Bme1580I G_kGCm'C
Cuts at: 2124 2402 3195 3288 4346 2124
Size: 278 793 93 1058 3350
Fragments arranged by size:
3350 1058 793 278 93
BmgBI CAC'GTC
Cuts at: 902 902
Size: 5572
BmrI ACTGGGnnnn_n'
Cuts at: 510 759 2396 2782 2863 3198 5344 510
Size: 249 1637 386 81 335 2146 738
Fragments arranged by size:
2146 1637 738 386 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1764 1920 3937 1764
Size: 156 2017 3399
Fragments arranged by size:
3399 2017 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 360 1183 1408 1747 279
Size: 81 823 225 339 4104
Fragments arranged by size:
4104 823 339 225 81
Bpu10I CC'TnA_GC
Cuts at: 1624 2272 1624
Size: 648 4924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 1234 2746 3625 4119 4417 934
Size: 300 1512 879 494 298 2089
Fragments arranged by size:
2089 1512 879 494 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 1884 5276 1884
Size: 3392 2180
BsaAI yAC'GTr
Cuts at: 2562 2923 2562
Size: 361 5211
BsaBI GATnn'nnATC
Cuts at: 6 1550 3084 5123 6
Size: 1544 1534 2039 455
Fragments arranged by size:
2039 1544 1534 455
BsaHI Gr'CG_yC
Cuts at: 3001 3249 3953 5449 3001
Size: 248 704 1496 3124
Fragments arranged by size:
3124 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2759 3280 3960 4234 4381 4792 2759
Size: 521 680 274 147 411 3539
Fragments arranged by size:
3539 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 181 199 211 897 927 1381 1411 2510
Size: 12 18 12 686 30 454 30 1099
Cuts at: 2510 2540 169
Size: 30 3201
Fragments arranged by size:
3201 1099 686 454 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 186 404 1180 1213 1492 1746 2002 2067 2126
Size: 218 776 33 279 254 256 65 59
Cuts at: 2126 186
Size: 3632
Fragments arranged by size:
3632 776 279 256 254 218 65 59
33
BseYI C'CCAG_C
Cuts at: 617 860 2995 3753 4332 4828 617
Size: 243 2135 758 579 496 1361
Fragments arranged by size:
2135 1361 758 579 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 859 1132 1666 859
Size: 273 534 4765
Fragments arranged by size:
4765 534 273
BsiEI CG_ry'CG
Cuts at: 104 3112 3158 4368 104
Size: 3008 46 1210 1308
Fragments arranged by size:
3008 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 139 1250 2584 3362 3552 4346 139
Size: 1111 1334 778 190 794 1365
Fragments arranged by size:
1365 1334 1111 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 139 1250 1738 2124 2402 2584 3195 3288 3362
Size: 1111 488 386 278 182 611 93 74
Cuts at: 3362 3552 3614 4346 139
Size: 190 62 732 1365
Fragments arranged by size:
1365 1111 732 611 488 386 278 190
182 93 74 62
BspEI T'CCGG_A
Cuts at: 4792 4792
Size: 5572
BspHI T'CATG_A
Cuts at: 2444 4748 2444
Size: 2304 3268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1131 2080 2620 2834 3136 3517 1131
Size: 949 540 214 302 381 3186
Fragments arranged by size:
3186 949 540 381 302 214
BsrBI CCG'CTC
Cuts at: 50 239 3862 3916 5402 5567 50
Size: 189 3623 54 1486 165 55
Fragments arranged by size:
3623 1486 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3482 3482
Size: 5572
BsrFI r'CCGG_y
Cuts at: 259 3568 3749 3960 259
Size: 3309 181 211 1871
Fragments arranged by size:
3309 1871 211 181
BssSI C'ACGA_G
Cuts at: 1705 3841 4201 1705
Size: 2136 360 3076
Fragments arranged by size:
3076 2136 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1146 2947 1146
Size: 1801 3771
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5572
BstXI CCAn_nnnn'nTGG
Cuts at: 523 523
Size: 5572
BstYI r'GATC_y
Cuts at: 1 301 945 1745 2472 2595 3062 3085 3420
Size: 300 644 800 727 123 467 23 335
Cuts at: 3420 3666 4680 4766 4778 5124 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 800 727 644 467 449 346 335
300 246 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1505 1505
Size: 5572
BtgI C'CryG_G
Cuts at: 576 1418 2115 576
Size: 842 697 4033
Fragments arranged by size:
4033 842 697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 296 3520 3701 3864 296
Size: 3224 181 163 2004
Fragments arranged by size:
3224 2004 181 163
BtsI GCAGTG_nn'
Cuts at: 609 1493 2192 2813 609
Size: 884 699 621 3368
Fragments arranged by size:
3368 884 699 621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1742 1777 1742
Size: 35 5537
DraI TTT'AAA
Cuts at: 2569 2569
Size: 5572
DraIII CAC_nnn'GTG
Cuts at: 892 1689 892
Size: 797 4775
DrdI GACnn_nn'nnGTC
Cuts at: 2184 3276 4136 2184
Size: 1092 860 3620
Fragments arranged by size:
3620 1092 860
EaeI y'GGCC_r
Cuts at: 579 1280 3155 3329 3720 3747 5094 579
Size: 701 1875 174 391 27 1347 1057
Fragments arranged by size:
1875 1347 1057 701 391 174 27
EagI C'GGCC_G
Cuts at: 3155 3155
Size: 5572
EarI CTCTTCn'nnn_
Cuts at: 210 456 1012 1256 1469 1915 2480 3593 3803
Size: 246 556 244 213 446 565 1113 210
Cuts at: 3803 210
Size: 1979
Fragments arranged by size:
1979 1113 565 556 446 246 244 213
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 189 231 3778 4090 4236 5382 189
Size: 42 3547 312 146 1146 379
Fragments arranged by size:
3547 1146 379 312 146 42
EcoICRI GAG'CTC
Cuts at: 137 1248 2582 137
Size: 1111 1334 3127
Fragments arranged by size:
3127 1334 1111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 360 719 1183 1408 1747 2403 2453 2504
Size: 81 359 464 225 339 656 50 51
Cuts at: 2504 3395 3827 4576 4844 279
Size: 891 432 749 268 1007
Fragments arranged by size:
1007 891 749 656 464 432 359 339
268 225 81 51 50
EcoO109I rG'GnC_Cy
Cuts at: 2095 2120 2121 2709 2095
Size: 25 1 588 4958
Fragments arranged by size:
4958 588 25 1
EcoRI G'AATT_C
Cuts at: 2574 2574
Size: 5572
EcoRV GAT'ATC
Cuts at: 1813 1813
Size: 5572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 413 681 1629 3589 413
Size: 268 948 1960 2396
Fragments arranged by size:
2396 1960 948 268
FspI TGC'GCA
Cuts at: 772 3351 772
Size: 2579 2993
FspAI rTGC'GCAy
Cuts at: 772 772
Size: 5572
HaeII r_GCGC'y
Cuts at: 1938 3004 3252 4276 5255 1938
Size: 1066 248 1024 979 2255
Fragments arranged by size:
2255 1066 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 399 431 897 929 1255 1287 1381 1413 1914
Size: 32 466 32 326 32 94 32 501
Cuts at: 1914 1946 2464 2496 3412 3442 3444 3474 399
Size: 32 518 32 916 30 2 30 2497
Fragments arranged by size:
2497 916 518 501 466 326 94 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 747 2609 747
Size: 1862 3710
HpaI GTT'AAC
Cuts at: 747 747
Size: 5572
KasI G'GCGC_C
Cuts at: 3000 3248 3000
Size: 248 5324
KpnI G_GTAC'C
Cuts at: 2590 2590
Size: 5572
MluI A'CGCG_T
Cuts at: 5005 5005
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 212 2259 4243 4427 188
Size: 24 2047 1984 184 1333
Fragments arranged by size:
2047 1984 1333 184 24
MscI TGG'CCA
Cuts at: 581 3331 581
Size: 2750 2822
MslI CAynn'nnrTG
Cuts at: 980 1003 3559 3686 980
Size: 23 2556 127 2866
Fragments arranged by size:
2866 2556 127 23
MspA1I CmG'CkG
Cuts at: 132 182 1011 1191 2681 2995 3355 4370 4615
Size: 50 829 180 1490 314 360 1015 245
Cuts at: 4615 132
Size: 1089
Fragments arranged by size:
1490 1089 1015 829 360 314 245 180
50
NaeI GCC'GGC
Cuts at: 261 3751 261
Size: 3490 2082
NarI GG'CG_CC
Cuts at: 3001 3249 3001
Size: 248 5324
NcoI C'CATG_G
Cuts at: 2115 2115
Size: 5572
NdeI CA'TA_TG
Cuts at: 1673 1673
Size: 5572
NgoMIV G'CCGG_C
Cuts at: 259 3749 259
Size: 3490 2082
NruI TCG'CGA
Cuts at: 3933 3933
Size: 5572
NsiI A_TGCA'T
Cuts at: 871 871
Size: 5572
NspI r_CATG'y
Cuts at: 869 2623 3123 4032 869
Size: 1754 500 909 2409
Fragments arranged by size:
2409 1754 909 500
PciI A'CATG_T
Cuts at: 4028 4028
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 690 690
Size: 5572
PfoI T'CCnGG_A
Cuts at: 1241 1241
Size: 5572
PmeI GTTT'AAAC
Cuts at: 2569 2569
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 169 201 4711 4743 169
Size: 32 4510 32 998
Fragments arranged by size:
4510 998 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2095
Size: 5572
PsiI TTA'TAA
Cuts at: 314 314
Size: 5572
PspOMI G'GGCC_C
Cuts at: 2120 2120
Size: 5572
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 653 685 653
Size: 32 5540
PstI C_TGCA'G
Cuts at: 828 2617 3302 828
Size: 1789 685 3098
Fragments arranged by size:
3098 1789 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5572
PvuII CAG'CTG
Cuts at: 1011 1191 2995 3355 1011
Size: 180 1804 360 3228
Fragments arranged by size:
3228 1804 360 180
RsrII CG'GwC_CG
Cuts at: 3765 3765
Size: 5572
SacI G_AGCT'C
Cuts at: 139 1250 2584 139
Size: 1111 1334 3127
Fragments arranged by size:
3127 1334 1111
SalI G'TCGA_C
Cuts at: 2607 2607
Size: 5572
SanDI GG'GwC_CC
Cuts at: 2095 2095
Size: 5572
SapI GCTCTTCn'nnn_
Cuts at: 456 3593 3803 456
Size: 3137 210 2225
Fragments arranged by size:
3137 2225 210
SbfI CC_TGCA'GG
Cuts at: 2617 2617
Size: 5572
ScaI AGT'ACT
Cuts at: 1111 5089 1111
Size: 3978 1594
SfcI C'TryA_G
Cuts at: 33 824 2041 2613 3298 4293 4484 33
Size: 791 1217 572 685 995 191 1121
Fragments arranged by size:
1217 1121 995 791 685 572 191
SfoI GGC'GCC
Cuts at: 3002 3250 3002
Size: 248 5324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5572
SmaI CCC'GGG
Cuts at: 2592 2592
Size: 5572
SmlI C'TyrA_G
Cuts at: 949 1249 2032 2725 3640 4134 4396 949
Size: 300 783 693 915 494 262 2125
Fragments arranged by size:
2125 915 783 693 494 300 262
SnaBI TAC'GTA
Cuts at: 2562 2562
Size: 5572
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5572
SphI G_CATG'C
Cuts at: 869 2623 3123 869
Size: 1754 500 3318
Fragments arranged by size:
3318 1754 500
StuI AGG'CCT
Cuts at: 1522 1522
Size: 5572
StyI C'CwwG_G
Cuts at: 1346 2115 2704 3058 1346
Size: 769 589 354 3860
Fragments arranged by size:
3860 769 589 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2205 3153 3286 3745 3956 2205
Size: 948 133 459 211 3821
Fragments arranged by size:
3821 948 459 211 133
TatI w'GTAC_w
Cuts at: 1109 5087 1109
Size: 3978 1594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5089 5121 5089
Size: 32 5540
Tth111I GACn'n_nGTC
Cuts at: 1802 3367 1802
Size: 1565 4007
XbaI T'CTAG_A
Cuts at: 1447 2601 1447
Size: 1154 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1122 1660 3065 1122
Size: 538 1405 3629
Fragments arranged by size:
3629 1405 538
XhoI C'TCGA_G
Cuts at: 2032 2032
Size: 5572
XmaI C'CCGG_G
Cuts at: 2590 2590
Size: 5572
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BglI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI EcoNI FseI HindIII MfeI NheI NotI PacI
PasI PmlI PshAI PspXI SacII SexAI SfiI SgrAI
SrfI SspI SwaI XmnI ZraI