Restriction map for
pF1KSDA0263
(Circular) (Six-base) MAPSORT of: pF1KSDA0263.seq Check: 8508 from: 1 to: 4460
pF1KSDA0263 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 906 906
Size: 4460
AccI GT'mk_AC
Cuts at: 1498 1498
Size: 4460
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2285 2717 3464 3732 820
Size: 1465 432 747 268 1548
Fragments arranged by size:
1548 1465 747 432 268
AfeI AGC'GCT
Cuts at: 295 295
Size: 4460
AflIII A'CryG_T
Cuts at: 830 1391 1812 2916 3893 830
Size: 561 421 1104 977 1397
Fragments arranged by size:
1397 1104 977 561 421
AgeI A'CCGG_T
Cuts at: 494 2848 494
Size: 2354 2106
AhdI GACnn_n'nnGTC
Cuts at: 1182 1182
Size: 4460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 4460
AlwNI CAG_nnn'CTG
Cuts at: 699 905 1765 3332 699
Size: 206 860 1567 1827
Fragments arranged by size:
1827 1567 860 206
ApaLI G'TGCA_C
Cuts at: 766 3230 766
Size: 2464 1996
ApoI r'AATT_y
Cuts at: 1464 3813 4069 1464
Size: 2349 256 1855
Fragments arranged by size:
2349 1855 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 284 1273 1480 284
Size: 989 207 3264
Fragments arranged by size:
3264 989 207
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 1476 1890 2138 2173 1476
Size: 414 248 35 3763
Fragments arranged by size:
3763 414 248 35
BanII G_rGCy'C
Cuts at: 209 562 977 1474 2504 209
Size: 353 415 497 1030 2165
Fragments arranged by size:
2165 1030 497 415 353
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3119 4460 2358
Size: 761 1341 2358
Fragments arranged by size:
2358 1341 761
BclI T'GATC_A
Cuts at: 1980 1980
Size: 4460
BglII A'GATC_T
Cuts at: 1975 4012 1975
Size: 2037 2423
BlpI GC'TnA_GC
Cuts at: 700 1572 700
Size: 872 3588
Bme1580I G_kGCm'C
Cuts at: 770 811 2085 2178 3234 770
Size: 41 1274 93 1056 1996
Fragments arranged by size:
1996 1274 1056 93 41
BmgBI CAC'GTC
Cuts at: 1039 1039
Size: 4460
BmrI ACTGGGnnnn_n'
Cuts at: 1672 1753 2088 4232 1672
Size: 81 335 2144 1900
Fragments arranged by size:
2144 1900 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 223 693 1104 1111 1423 203
Size: 20 470 411 7 312 3240
Fragments arranged by size:
3240 470 411 312 20 7
Bpu10I CC'TnA_GC
Cuts at: 1060 1060
Size: 4460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 663 775 1183 1636 2515 3007 3305 565
Size: 98 112 408 453 879 492 298 1720
Fragments arranged by size:
1720 879 492 453 408 298 112 98
BsaI GGTCTCn'nnnn_
Cuts at: 179 541 4164 179
Size: 362 3623 475
Fragments arranged by size:
3623 475 362
BsaAI yAC'GTr
Cuts at: 1392 1452 1813 1392
Size: 60 361 4039
Fragments arranged by size:
4039 361 60
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 896 1231 1891 2139 2841 4337 896
Size: 335 660 248 702 1496 1019
Fragments arranged by size:
1496 1019 702 660 335 248
BsaWI w'CCGG_w
Cuts at: 494 1649 2170 2848 3122 3269 3680 494
Size: 1155 521 678 274 147 411 1274
Fragments arranged by size:
1274 1155 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 666 696 666
Size: 30 4430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 1278 538
Size: 740 3720
BseYI C'CCAG_C
Cuts at: 891 1246 1258 1885 2643 3220 3716 891
Size: 355 12 627 758 577 496 1635
Fragments arranged by size:
1635 758 627 577 496 355 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 577 1000 475
Size: 102 423 3935
Fragments arranged by size:
3935 423 102
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 562 770 880 1220 1474 2252 2442 3234 562
Size: 208 110 340 254 778 190 792 1788
Fragments arranged by size:
1788 792 778 340 254 208 190 110
BsmI GAATG_Cn'
Cuts at: 174 174
Size: 4460
BsmBI CGTCTCn'nnnn_
Cuts at: 1201 1201
Size: 4460
Bsp1286I G_dGCh'C
Cuts at: 209 562 770 811 880 977 1220 1474 2085
Size: 353 208 41 69 97 243 254 611
Cuts at: 2085 2178 2252 2442 2504 3234 209
Size: 93 74 190 62 730 1435
Fragments arranged by size:
1435 730 611 353 254 243 208 190
97 93 74 69 62 41
BspEI T'CCGG_A
Cuts at: 3680 3680
Size: 4460
BspHI T'CATG_A
Cuts at: 3636 3636
Size: 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 906 1040 1510 1724 2026 2407 906
Size: 134 470 214 302 381 2959
Fragments arranged by size:
2959 470 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 1095 2752 2806 4290 4455 50
Size: 1045 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 1045 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1002 2372 1002
Size: 1370 3090
BsrFI r'CCGG_y
Cuts at: 494 1181 2458 2639 2848 494
Size: 687 1277 181 209 2106
Fragments arranged by size:
2106 1277 687 209 181
BssSI C'ACGA_G
Cuts at: 2731 3089 2731
Size: 358 4102
BstAPI GCAn_nnn'nTGC
Cuts at: 1154 1837 1154
Size: 683 3777
BstBI TT'CG_AA
Cuts at: 110 2821 110
Size: 2711 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 150 150
Size: 4460
BstYI r'GATC_y
Cuts at: 1 1485 1952 1975 2310 2556 3568 3654 3666
Size: 1484 467 23 335 246 1012 86 12
Cuts at: 3666 4012 1
Size: 346 449
Fragments arranged by size:
1484 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1223 1223
Size: 4460
BtgI C'CryG_G
Cuts at: 143 969 143
Size: 826 3634
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 839 2410 2591 2754 839
Size: 1571 181 163 2545
Fragments arranged by size:
2545 1571 181 163
BtsI GCAGTG_nn'
Cuts at: 510 516 1703 510
Size: 6 1187 3267
Fragments arranged by size:
3267 1187 6
DraI TTT'AAA
Cuts at: 1459 1459
Size: 4460
DraIII CAC_nnn'GTG
Cuts at: 264 266 264
Size: 2 4458
DrdI GACnn_nn'nnGTC
Cuts at: 193 2166 3024 193
Size: 1973 858 1629
Fragments arranged by size:
1973 1629 858
EaeI y'GGCC_r
Cuts at: 966 2045 2219 2610 2637 3982 966
Size: 1079 174 391 27 1345 1444
Fragments arranged by size:
1444 1345 1079 391 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 242 2668 2978 3124 4270 242
Size: 2426 310 146 1146 432
Fragments arranged by size:
2426 1146 432 310 146
EcoICRI GAG'CTC
Cuts at: 560 1472 560
Size: 912 3548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 223 693 820 1104 1111 1423 2285 2717
Size: 20 470 127 284 7 312 862 432
Cuts at: 2717 3464 3732 203
Size: 747 268 931
Fragments arranged by size:
931 862 747 470 432 312 284 268
127 20 7
EcoO109I rG'GnC_Cy
Cuts at: 609 1074 1282 1599 609
Size: 465 208 317 3470
Fragments arranged by size:
3470 465 317 208
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4460
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 297 1894 2142 3164 4143 297
Size: 1597 248 1022 979 614
Fragments arranged by size:
1597 1022 979 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 1168 1200 1216 1248 2302 2332 2334
Size: 32 319 32 16 32 1054 30 2
Cuts at: 2334 2364 817
Size: 30 2913
Fragments arranged by size:
2913 1054 319 32 32 32 30 30
16 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 830 3893 830
Size: 3063 1397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 773 3131 3315 773
Size: 2358 184 1918
Fragments arranged by size:
2358 1918 184
MscI TGG'CCA
Cuts at: 2221 2221
Size: 4460
MslI CAynn'nnrTG
Cuts at: 148 824 916 2449 2576 148
Size: 676 92 1533 127 2032
Fragments arranged by size:
2032 1533 676 127 92
MspA1I CmG'CkG
Cuts at: 699 838 927 1571 1885 2245 3258 3503 699
Size: 139 89 644 314 360 1013 245 1656
Fragments arranged by size:
1656 1013 644 360 314 245 139 89
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NcoI C'CATG_G
Cuts at: 143 143
Size: 4460
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 711 915 1513 2013 2920 711
Size: 204 598 500 907 2251
Fragments arranged by size:
2251 907 598 500 204
PciI A'CATG_T
Cuts at: 2916 2916
Size: 4460
PfoI T'CCnGG_A
Cuts at: 200 1349 200
Size: 1149 3311
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 609 1282 609
Size: 673 3787
PstI C_TGCA'G
Cuts at: 952 1507 2192 952
Size: 555 685 3220
Fragments arranged by size:
3220 685 555
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 699 838 927 1885 2245 699
Size: 139 89 958 360 2914
Fragments arranged by size:
2914 958 360 139 89
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 562 1474 562
Size: 912 3548
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SanDI GG'GwC_CC
Cuts at: 1282 1282
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 961 3977 961
Size: 3016 1444
SfcI C'TryA_G
Cuts at: 33 948 1503 2188 3181 3372 33
Size: 915 555 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 555 191
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 286 1482 286
Size: 1196 3264
SmlI C'TyrA_G
Cuts at: 544 642 754 1198 1615 2530 3022 3284 544
Size: 98 112 444 417 915 492 262 1720
Fragments arranged by size:
1720 915 492 444 417 262 112 98
SnaBI TAC'GTA
Cuts at: 1452 1452
Size: 4460
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 711 915 1513 2013 711
Size: 204 598 500 3158
Fragments arranged by size:
3158 598 500 204
StuI AGG'CCT
Cuts at: 1102 1102
Size: 4460
StyI C'CwwG_G
Cuts at: 143 722 1298 1594 1948 143
Size: 579 576 296 354 2655
Fragments arranged by size:
2655 579 576 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 743 2043 2176 2635 2844 743
Size: 1300 133 459 209 2359
Fragments arranged by size:
2359 1300 459 209 133
TatI w'GTAC_w
Cuts at: 959 3975 959
Size: 3016 1444
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4428
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 625 1955 625
Size: 1330 3130
XmaI C'CCGG_G
Cuts at: 284 1480 284
Size: 1196 3264
XmnI GAAnn'nnTTC
Cuts at: 328 328
Size: 4460
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AleI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI SspI
SwaI XhoI ZraI