Restriction map for
pF1KSDA0264
(Circular) (Six-base) MAPSORT of: pF1KSDA0264.seq Check: 5426 from: 1 to: 4381
pF1KSDA0264 4381 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 230 1417 230
Size: 1187 3194
Acc65I G'GTAC_C
Cuts at: 431 1395 431
Size: 964 3417
AclI AA'CG_TT
Cuts at: 4221 4221
Size: 4381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 696 983 998 1006 2204 2636 3385 3653
Size: 493 287 15 8 1198 432 749 268
Cuts at: 3653 203
Size: 931
Fragments arranged by size:
1198 931 749 493 432 287 268 15
8
AfeI AGC'GCT
Cuts at: 962 962
Size: 4381
AflIII A'CryG_T
Cuts at: 1731 2837 3814 1731
Size: 1106 977 2298
Fragments arranged by size:
2298 1106 977
AgeI A'CCGG_T
Cuts at: 2769 2769
Size: 4381
AlwNI CAG_nnn'CTG
Cuts at: 1260 1684 3253 1260
Size: 424 1569 2388
Fragments arranged by size:
2388 1569 424
ApaLI G'TGCA_C
Cuts at: 3151 3151
Size: 4381
ApoI r'AATT_y
Cuts at: 533 1383 3734 3990 533
Size: 850 2351 256 924
Fragments arranged by size:
2351 924 850 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4381
AvaI C'yCGr_G
Cuts at: 368 1399 368
Size: 1031 3350
BamHI G'GATC_C
Cuts at: 1404 1404
Size: 4381
BanI G'GyrC_C
Cuts at: 431 1395 1809 2057 2092 431
Size: 964 414 248 35 2720
Fragments arranged by size:
2720 964 414 248 35
BanII G_rGCy'C
Cuts at: 903 1393 2423 903
Size: 490 1030 2861
Fragments arranged by size:
2861 1030 490
BbeI G_GCGC'C
Cuts at: 1813 2061 1813
Size: 248 4133
BbsI GAAGACnn'nnnn_
Cuts at: 709 944 1046 709
Size: 235 102 4044
Fragments arranged by size:
4044 235 102
BbvCI CC'TCA_GC
Cuts at: 182 182
Size: 4381
BciVI GTATCCnnnnn_n'
Cuts at: 2277 3040 4381 2277
Size: 763 1341 2277
Fragments arranged by size:
2277 1341 763
BclI T'GATC_A
Cuts at: 1899 1899
Size: 4381
BglII A'GATC_T
Cuts at: 1894 3933 1894
Size: 2039 2342
BlpI GC'TnA_GC
Cuts at: 1491 1491
Size: 4381
Bme1580I G_kGCm'C
Cuts at: 2004 2097 3155 2004
Size: 93 1058 3230
Fragments arranged by size:
3230 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 789 1591 1672 2007 4153 789
Size: 802 81 335 2146 1017
Fragments arranged by size:
2146 1017 802 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 979 2746 979
Size: 1767 2614
Bpu10I CC'TnA_GC
Cuts at: 182 182
Size: 4381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1555 2434 2928 3226 1555
Size: 879 494 298 2710
Fragments arranged by size:
2710 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4085 4085
Size: 4381
BsaAI yAC'GTr
Cuts at: 1371 1732 1371
Size: 361 4020
BsaBI GATnn'nnATC
Cuts at: 6 1893 3932 6
Size: 1887 2039 455
Fragments arranged by size:
2039 1887 455
BsaHI Gr'CG_yC
Cuts at: 1810 2058 2762 4258 1810
Size: 248 704 1496 1933
Fragments arranged by size:
1933 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1568 2089 2769 3043 3190 3601 1568
Size: 521 680 274 147 411 2348
Fragments arranged by size:
2348 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 739 1033 739
Size: 294 4087
BseYI C'CCAG_C
Cuts at: 1196 1804 2562 3141 3637 1196
Size: 608 758 579 496 1940
Fragments arranged by size:
1940 758 608 579 496
BsiEI CG_ry'CG
Cuts at: 104 1921 1967 3177 104
Size: 1817 46 1210 1308
Fragments arranged by size:
1817 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1393 2171 2361 3155 1393
Size: 778 190 794 2619
Fragments arranged by size:
2619 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 903 1393 2004 2097 2171 2361 2423 3155 903
Size: 490 611 93 74 190 62 732 2129
Fragments arranged by size:
2129 732 611 490 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3601 3601
Size: 4381
BspHI T'CATG_A
Cuts at: 626 3557 626
Size: 2931 1450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 218 1429 1643 1945 2326 187
Size: 31 1211 214 302 381 2242
Fragments arranged by size:
2242 1211 381 302 214 31
BsrBI CCG'CTC
Cuts at: 50 2671 2725 4211 4376 50
Size: 2621 54 1486 165 55
Fragments arranged by size:
2621 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 687 2291 687
Size: 1604 2777
BsrFI r'CCGG_y
Cuts at: 2377 2558 2769 2377
Size: 181 211 3989
Fragments arranged by size:
3989 211 181
BsrGI T'GTAC_A
Cuts at: 1284 1284
Size: 4381
BssSI C'ACGA_G
Cuts at: 2650 3010 2650
Size: 360 4021
BstAPI GCAn_nnn'nTGC
Cuts at: 1260 1756 1260
Size: 496 3885
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4381
BstXI CCAn_nnnn'nTGG
Cuts at: 802 802
Size: 4381
BstYI r'GATC_y
Cuts at: 1 1404 1871 1894 2229 2475 3489 3575 3587
Size: 1403 467 23 335 246 1014 86 12
Cuts at: 3587 3933 1
Size: 346 449
Fragments arranged by size:
1403 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1224 2329 2510 2673 1224
Size: 1105 181 163 2932
Fragments arranged by size:
2932 1105 181 163
BtsI GCAGTG_nn'
Cuts at: 475 986 1273 1622 475
Size: 511 287 349 3234
Fragments arranged by size:
3234 511 349 287
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1182 1217 1182
Size: 35 4346
DraI TTT'AAA
Cuts at: 1378 1378
Size: 4381
DrdI GACnn_nn'nnGTC
Cuts at: 2085 2945 2085
Size: 860 3521
EaeI y'GGCC_r
Cuts at: 1964 2138 2529 2556 3903 1964
Size: 174 391 27 1347 2442
Fragments arranged by size:
2442 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1964 1964
Size: 4381
EarI CTCTTCn'nnn_
Cuts at: 368 716 2402 2612 368
Size: 348 1686 210 2137
Fragments arranged by size:
2137 1686 348 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2587 2899 3045 4191 2587
Size: 312 146 1146 2777
Fragments arranged by size:
2777 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1391 1391
Size: 4381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 696 983 998 1006 2204 2636 3385 3653
Size: 493 287 15 8 1198 432 749 268
Cuts at: 3653 203
Size: 931
Fragments arranged by size:
1198 931 749 493 432 287 268 15
8
EcoNI CCTnn'n_nnAGG
Cuts at: 462 462
Size: 4381
EcoO109I rG'GnC_Cy
Cuts at: 1518 1518
Size: 4381
EcoRI G'AATT_C
Cuts at: 1383 1383
Size: 4381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 193 726 2398 193
Size: 533 1672 2176
Fragments arranged by size:
2176 1672 533
FspI TGC'GCA
Cuts at: 2160 2160
Size: 4381
HaeII r_GCGC'y
Cuts at: 964 1813 2061 3085 4064 964
Size: 849 248 1024 979 1281
Fragments arranged by size:
1281 1024 979 849 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 592 624 1009 1041 2221 2251 2253
Size: 32 289 32 385 32 1180 30 2
Cuts at: 2253 2283 271
Size: 30 2369
Fragments arranged by size:
2369 1180 385 289 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1418 1418
Size: 4381
HindIII A'AGCT_T
Cuts at: 1102 1144 1102
Size: 42 4339
KasI G'GCGC_C
Cuts at: 1809 2057 1809
Size: 248 4133
KpnI G_GTAC'C
Cuts at: 435 1399 435
Size: 964 3417
MluI A'CGCG_T
Cuts at: 3814 3814
Size: 4381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3052 3236 3052
Size: 184 4197
MscI TGG'CCA
Cuts at: 2140 2140
Size: 4381
MslI CAynn'nnrTG
Cuts at: 2368 2495 2368
Size: 127 4254
MspA1I CmG'CkG
Cuts at: 1003 1490 1804 2164 3179 3424 1003
Size: 487 314 360 1015 245 1960
Fragments arranged by size:
1960 1015 487 360 314 245
NaeI GCC'GGC
Cuts at: 2560 2560
Size: 4381
NarI GG'CG_CC
Cuts at: 1810 2058 1810
Size: 248 4133
NcoI C'CATG_G
Cuts at: 125 125
Size: 4381
NgoMIV G'CCGG_C
Cuts at: 2558 2558
Size: 4381
NruI TCG'CGA
Cuts at: 2742 2742
Size: 4381
NspI r_CATG'y
Cuts at: 867 1432 1932 2841 867
Size: 565 500 909 2407
Fragments arranged by size:
2407 909 565 500
PciI A'CATG_T
Cuts at: 2837 2837
Size: 4381
PflMI CCAn_nnn'nTGG
Cuts at: 428 428
Size: 4381
PmeI GTTT'AAAC
Cuts at: 1378 1378
Size: 4381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3520 3552 3520
Size: 32 4349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 506 538 506
Size: 32 4349
PstI C_TGCA'G
Cuts at: 198 1265 1426 2111 198
Size: 1067 161 685 2468
Fragments arranged by size:
2468 1067 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4381
PvuII CAG'CTG
Cuts at: 1003 1804 2164 1003
Size: 801 360 3220
Fragments arranged by size:
3220 801 360
RsrII CG'GwC_CG
Cuts at: 2574 2574
Size: 4381
SacI G_AGCT'C
Cuts at: 1393 1393
Size: 4381
SalI G'TCGA_C
Cuts at: 1416 1416
Size: 4381
SapI GCTCTTCn'nnn_
Cuts at: 2402 2612 2402
Size: 210 4171
SbfI CC_TGCA'GG
Cuts at: 198 1426 198
Size: 1228 3153
ScaI AGT'ACT
Cuts at: 3898 3898
Size: 4381
SfcI C'TryA_G
Cuts at: 33 194 1261 1422 2107 3102 3293 33
Size: 161 1067 161 685 995 191 1121
Fragments arranged by size:
1121 1067 995 685 191 161 161
SfoI GGC'GCC
Cuts at: 1811 2059 1811
Size: 248 4133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4381
SmaI CCC'GGG
Cuts at: 1401 1401
Size: 4381
SmlI C'TyrA_G
Cuts at: 1534 2449 2943 3205 1534
Size: 915 494 262 2710
Fragments arranged by size:
2710 915 494 262
SnaBI TAC'GTA
Cuts at: 1371 1371
Size: 4381
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4381
SphI G_CATG'C
Cuts at: 1432 1932 1432
Size: 500 3881
StuI AGG'CCT
Cuts at: 761 1132 761
Size: 371 4010
StyI C'CwwG_G
Cuts at: 125 1513 1867 125
Size: 1388 354 2639
Fragments arranged by size:
2639 1388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 673 1210 1962 2095 2554 2765 673
Size: 537 752 133 459 211 2289
Fragments arranged by size:
2289 752 537 459 211 133
TatI w'GTAC_w
Cuts at: 1284 3896 1284
Size: 2612 1769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 723 755 3898 3930 723
Size: 32 3143 32 1174
Fragments arranged by size:
3143 1174 32 32
Tth111I GACn'n_nGTC
Cuts at: 2176 2176
Size: 4381
XbaI T'CTAG_A
Cuts at: 1275 1410 1275
Size: 135 4246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1874 1874
Size: 4381
XmaI C'CCGG_G
Cuts at: 1399 1399
Size: 4381
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI