Restriction map for
pF1KSDA0269
(Circular) (Six-base) MAPSORT of: pF1KSDA0269.seq Check: 1373 from: 1 to: 4816
pF1KSDA0269 4816 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1852 1852
Size: 4816
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4816
AclI AA'CG_TT
Cuts at: 4656 4656
Size: 4816
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 300 589 788 1234 2639 3071 3820 4088
Size: 10 289 199 446 1405 432 749 268
Cuts at: 4088 290
Size: 1018
Fragments arranged by size:
1405 1018 749 446 432 289 268 199
10
AflIII A'CryG_T
Cuts at: 339 2166 3272 4249 339
Size: 1827 1106 977 906
Fragments arranged by size:
1827 1106 977 906
AgeI A'CCGG_T
Cuts at: 3204 3204
Size: 4816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 4816
AlwNI CAG_nnn'CTG
Cuts at: 744 1207 1392 2119 3688 744
Size: 463 185 727 1569 1872
Fragments arranged by size:
1872 1569 727 463 185
ApaLI G'TGCA_C
Cuts at: 3586 3586
Size: 4816
ApoI r'AATT_y
Cuts at: 1777 1818 4169 4425 1777
Size: 41 2351 256 2168
Fragments arranged by size:
2351 2168 256 41
AseI AT'TA_AT
Cuts at: 20 1531 20
Size: 1511 3305
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4816
AvaI C'yCGr_G
Cuts at: 1834 1834
Size: 4816
AvrII C'CTAG_G
Cuts at: 154 154
Size: 4816
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4816
BanI G'GyrC_C
Cuts at: 1830 2244 2492 2527 1830
Size: 414 248 35 4119
Fragments arranged by size:
4119 414 248 35
BanII G_rGCy'C
Cuts at: 1090 1828 2858 1090
Size: 738 1030 3048
Fragments arranged by size:
3048 1030 738
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4568
BbvCI CC'TCA_GC
Cuts at: 926 926
Size: 4816
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3475 4816 2712
Size: 763 1341 2712
Fragments arranged by size:
2712 1341 763
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4816
BfrBI ATG'CAT
Cuts at: 978 978
Size: 4816
BglII A'GATC_T
Cuts at: 2329 4368 2329
Size: 2039 2777
BlpI GC'TnA_GC
Cuts at: 1691 1926 1691
Size: 235 4581
Bme1580I G_kGCm'C
Cuts at: 2439 2532 3590 2439
Size: 93 1058 3665
Fragments arranged by size:
3665 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1333 2026 2107 2442 4588 1333
Size: 693 81 335 2146 1561
Fragments arranged by size:
2146 1561 693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1109 3181 1109
Size: 2072 2744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1184 1205 1223 1283 1160
Size: 24 21 18 60 4693
Fragments arranged by size:
4693 60 24 21 18
Bpu10I CC'TnA_GC
Cuts at: 926 926
Size: 4816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2869 3363 3661 1990
Size: 879 494 298 3145
Fragments arranged by size:
3145 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4520 4520
Size: 4816
BsaAI yAC'GTr
Cuts at: 852 1806 2167 852
Size: 954 361 3501
Fragments arranged by size:
3501 954 361
BsaBI GATnn'nnATC
Cuts at: 6 779 2328 4367 6
Size: 773 1549 2039 455
Fragments arranged by size:
2039 1549 773 455
BsaHI Gr'CG_yC
Cuts at: 2245 2493 3197 4693 2245
Size: 248 704 1496 2368
Fragments arranged by size:
2368 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2003 2524 3204 3478 3625 4036 2003
Size: 521 680 274 147 411 2783
Fragments arranged by size:
2783 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1149 1179 1149
Size: 30 4786
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1094 1157 1235 1271 1274 1289 1406 1535 1094
Size: 63 78 36 3 15 117 129 4375
Fragments arranged by size:
4375 129 117 78 63 36 15 3
BseYI C'CCAG_C
Cuts at: 816 1275 2239 2997 3576 4072 816
Size: 459 964 758 579 496 1560
Fragments arranged by size:
1560 964 758 579 496 459
BsiEI CG_ry'CG
Cuts at: 104 465 2356 2402 3612 104
Size: 361 1891 46 1210 1308
Fragments arranged by size:
1891 1308 1210 361 46
BsiHKAI G_wGCw'C
Cuts at: 714 1828 2606 2796 3590 714
Size: 1114 778 190 794 1940
Fragments arranged by size:
1940 1114 794 778 190
BsiWI C'GTAC_G
Cuts at: 497 497
Size: 4816
BsmI GAATG_Cn'
Cuts at: 1433 1433
Size: 4816
BsmBI CGTCTCn'nnnn_
Cuts at: 487 487
Size: 4816
Bsp1286I G_dGCh'C
Cuts at: 714 1090 1828 2439 2532 2606 2796 2858 3590
Size: 376 738 611 93 74 190 62 732
Cuts at: 3590 714
Size: 1940
Fragments arranged by size:
1940 738 732 611 376 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4036 4036
Size: 4816
BspHI T'CATG_A
Cuts at: 687 717 3992 687
Size: 30 3275 1511
Fragments arranged by size:
3275 1511 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1572 1864 2078 2380 2761 1572
Size: 292 214 302 381 3627
Fragments arranged by size:
3627 381 302 292 214
BsrBI CCG'CTC
Cuts at: 50 3106 3160 4646 4811 50
Size: 3056 54 1486 165 55
Fragments arranged by size:
3056 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 328 2726 328
Size: 2398 2418
BsrFI r'CCGG_y
Cuts at: 2812 2993 3204 2812
Size: 181 211 4424
Fragments arranged by size:
4424 211 181
BssSI C'ACGA_G
Cuts at: 3085 3445 3085
Size: 360 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 991 2191 991
Size: 1200 3616
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4816
BstYI r'GATC_y
Cuts at: 1 868 1839 2306 2329 2664 2910 3924 4010
Size: 867 971 467 23 335 246 1014 86
Cuts at: 4010 4022 4368 1
Size: 12 346 449
Fragments arranged by size:
1014 971 867 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4472
Fragments arranged by size:
4472 181 163
BtsI GCAGTG_nn'
Cuts at: 175 1205 2057 175
Size: 1030 852 2934
Fragments arranged by size:
2934 1030 852
ClaI AT'CG_AT
Cuts at: 149 149
Size: 4816
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4816
DraIII CAC_nnn'GTG
Cuts at: 1378 1378
Size: 4816
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3380 2520
Size: 860 3956
EaeI y'GGCC_r
Cuts at: 2399 2573 2964 2991 4338 2399
Size: 174 391 27 1347 2877
Fragments arranged by size:
2877 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4816
EarI CTCTTCn'nnn_
Cuts at: 1121 1253 1664 2837 3047 1121
Size: 132 411 1173 210 2890
Fragments arranged by size:
2890 1173 411 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3334 3480 4626 3022
Size: 312 146 1146 3212
Fragments arranged by size:
3212 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 300 589 788 1160 1184 1205 1223 1234
Size: 10 289 199 372 24 21 18 11
Cuts at: 1234 1283 2639 3071 3820 4088 290
Size: 49 1356 432 749 268 1018
Fragments arranged by size:
1356 1018 749 432 372 289 268 199
49 24 21 18 11 10
EcoO109I rG'GnC_Cy
Cuts at: 1516 1953 1516
Size: 437 4379
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4816
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4816
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4816
HaeII r_GCGC'y
Cuts at: 1584 2248 2496 3520 4499 1584
Size: 664 248 1024 979 1901
Fragments arranged by size:
1901 1024 979 664 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 1026 1058 1149 1181 2656 2686 2688
Size: 32 657 32 91 32 1475 30 2
Cuts at: 2688 2718 337
Size: 30 2435
Fragments arranged by size:
2435 1475 657 91 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 324 1133 1853 324
Size: 809 720 3287
Fragments arranged by size:
3287 809 720
HindIII A'AGCT_T
Cuts at: 422 422
Size: 4816
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4568
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4816
MfeI C'AATT_G
Cuts at: 1289 1289
Size: 4816
MluI A'CGCG_T
Cuts at: 4249 4249
Size: 4816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1519 1778 3487 3671 1519
Size: 259 1709 184 2664
Fragments arranged by size:
2664 1709 259 184
MscI TGG'CCA
Cuts at: 2575 2575
Size: 4816
MslI CAynn'nnrTG
Cuts at: 863 2803 2930 863
Size: 1940 127 2749
Fragments arranged by size:
2749 1940 127
MspA1I CmG'CkG
Cuts at: 1925 2239 2599 3614 3859 1925
Size: 314 360 1015 245 2882
Fragments arranged by size:
2882 1015 360 314 245
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4816
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4568
NdeI CA'TA_TG
Cuts at: 860 860
Size: 4816
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4816
NruI TCG'CGA
Cuts at: 3177 3177
Size: 4816
NsiI A_TGCA'T
Cuts at: 980 980
Size: 4816
NspI r_CATG'y
Cuts at: 1867 2367 3276 1867
Size: 500 909 3407
Fragments arranged by size:
3407 909 500
PciI A'CATG_T
Cuts at: 3272 3272
Size: 4816
PflMI CCAn_nnn'nTGG
Cuts at: 979 979
Size: 4816
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3955 3987 3955
Size: 32 4784
PpuMI rG'GwC_Cy
Cuts at: 1516 1516
Size: 4816
PstI C_TGCA'G
Cuts at: 1861 2546 1861
Size: 685 4131
PvuI CG_AT'CG
Cuts at: 104 465 104
Size: 361 4455
PvuII CAG'CTG
Cuts at: 2239 2599 2239
Size: 360 4456
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4816
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4816
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4816
SapI GCTCTTCn'nnn_
Cuts at: 1664 2837 3047 1664
Size: 1173 210 3433
Fragments arranged by size:
3433 1173 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4816
ScaI AGT'ACT
Cuts at: 4333 4333
Size: 4816
SfcI C'TryA_G
Cuts at: 33 1025 1061 1857 2542 3537 3728 33
Size: 992 36 796 685 995 191 1121
Fragments arranged by size:
1121 995 992 796 685 191 36
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4568
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4816
SmaI CCC'GGG
Cuts at: 1836 1836
Size: 4816
SmlI C'TyrA_G
Cuts at: 1969 2884 3378 3640 1969
Size: 915 494 262 3145
Fragments arranged by size:
3145 915 494 262
SnaBI TAC'GTA
Cuts at: 1806 1806
Size: 4816
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4816
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4316
SspI AAT'ATT
Cuts at: 219 219
Size: 4816
StyI C'CwwG_G
Cuts at: 154 224 750 1948 2302 154
Size: 70 526 1198 354 2668
Fragments arranged by size:
2668 1198 526 354 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1289 2397 2530 2989 3200 1289
Size: 1108 133 459 211 2905
Fragments arranged by size:
2905 1108 459 211 133
TatI w'GTAC_w
Cuts at: 1305 4331 1305
Size: 3026 1790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4333 4365 4333
Size: 32 4784
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4816
XbaI T'CTAG_A
Cuts at: 675 1845 675
Size: 1170 3646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 2309 222
Size: 2087 2729
XmaI C'CCGG_G
Cuts at: 1834 1834
Size: 4816
XmnI GAAnn'nnTTC
Cuts at: 424 424
Size: 4816
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NruI NsiI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI BaeI BbsI BcgI BglI BmgBI BmtI BsgI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI
EcoNI EcoRV FseI FspAI HpaI NcoI NheI NotI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI