Restriction map for
pF1KSDA0271
(Circular) (Six-base) MAPSORT of: pF1KSDA0271.seq Check: 9084 from: 1 to: 3718
pF1KSDA0271 3718 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 754 754
Size: 3718
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3718
AclI AA'CG_TT
Cuts at: 3558 3558
Size: 3718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2722 2990 1541
Size: 432 749 268 2269
Fragments arranged by size:
2269 749 432 268
AflIII A'CryG_T
Cuts at: 1068 2174 3151 1068
Size: 1106 977 1635
Fragments arranged by size:
1635 1106 977
AgeI A'CCGG_T
Cuts at: 2106 2106
Size: 3718
AleI CACnn'nnGTG
Cuts at: 545 545
Size: 3718
AlwNI CAG_nnn'CTG
Cuts at: 241 1021 2590 241
Size: 780 1569 1369
Fragments arranged by size:
1569 1369 780
ApaI G_GGCC'C
Cuts at: 235 397 591 672 235
Size: 162 194 81 3281
Fragments arranged by size:
3281 194 162 81
ApaLI G'TGCA_C
Cuts at: 2488 2488
Size: 3718
ApoI r'AATT_y
Cuts at: 720 3071 3327 720
Size: 2351 256 1111
Fragments arranged by size:
2351 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3718
AvaI C'yCGr_G
Cuts at: 223 736 223
Size: 513 3205
BamHI G'GATC_C
Cuts at: 536 741 536
Size: 205 3513
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3021
Fragments arranged by size:
3021 414 248 35
BanII G_rGCy'C
Cuts at: 162 235 397 591 672 730 1760 162
Size: 73 162 194 81 58 1030 2120
Fragments arranged by size:
2120 1030 194 162 81 73 58
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3470
BbvCI CC'TCA_GC
Cuts at: 190 190
Size: 3718
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2377 3718 1614
Size: 763 1341 1614
Fragments arranged by size:
1614 1341 763
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3718
BglI GCCn_nnn'nGGC
Cuts at: 265 265
Size: 3718
BglII A'GATC_T
Cuts at: 1231 3270 1231
Size: 2039 1679
BlpI GC'TnA_GC
Cuts at: 323 345 828 323
Size: 22 483 3213
Fragments arranged by size:
3213 483 22
Bme1580I G_kGCm'C
Cuts at: 235 397 591 672 1341 1434 2492 235
Size: 162 194 81 669 93 1058 1461
Fragments arranged by size:
1461 1058 669 194 162 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 411 633 673 928 1009 1344 3490 411
Size: 222 40 255 81 335 2146 639
Fragments arranged by size:
2146 639 335 255 222 81 40
BmtI G_CTAG'C
Cuts at: 701 701
Size: 3718
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 535 613 293
Size: 242 78 3398
Fragments arranged by size:
3398 242 78
Bpu10I CC'TnA_GC
Cuts at: 190 190
Size: 3718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2265 2563 892
Size: 879 494 298 2047
Fragments arranged by size:
2047 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 280 375 3422 280
Size: 95 3047 576
Fragments arranged by size:
3047 576 95
BsaAI yAC'GTr
Cuts at: 708 1069 708
Size: 361 3357
BsaBI GATnn'nnATC
Cuts at: 6 1230 3269 6
Size: 1224 2039 455
Fragments arranged by size:
2039 1224 455
BsaHI Gr'CG_yC
Cuts at: 606 1147 1395 2099 3595 606
Size: 541 248 704 1496 729
Fragments arranged by size:
1496 729 704 541 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2106 2380 2527 2938 905
Size: 521 680 274 147 411 1685
Fragments arranged by size:
1685 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 610
Size: 3718
BseYI C'CCAG_C
Cuts at: 135 234 554 1141 1899 2478 2974 135
Size: 99 320 587 758 579 496 879
Fragments arranged by size:
879 758 587 579 496 320 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 422 511 235
Size: 187 89 3442
Fragments arranged by size:
3442 187 89
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2514 104
Size: 1154 46 1210 1308
Fragments arranged by size:
1308 1210 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2492 730
Size: 778 190 794 1956
Fragments arranged by size:
1956 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 511 511
Size: 3718
Bsp1286I G_dGCh'C
Cuts at: 162 235 397 591 672 730 1341 1434 1508
Size: 73 162 194 81 58 611 93 74
Cuts at: 1508 1698 1760 2492 162
Size: 190 62 732 1388
Fragments arranged by size:
1388 732 611 194 190 162 93 81
74 73 62 58
BspEI T'CCGG_A
Cuts at: 2938 2938
Size: 3718
BspHI T'CATG_A
Cuts at: 2894 2894
Size: 3718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2821
Fragments arranged by size:
2821 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3548 3713 50
Size: 1958 54 1486 165 55
Fragments arranged by size:
1958 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3718
BsrFI r'CCGG_y
Cuts at: 1714 1895 2106 1714
Size: 181 211 3326
Fragments arranged by size:
3326 211 181
BssSI C'ACGA_G
Cuts at: 1987 2347 1987
Size: 360 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 445 1093 445
Size: 648 3070
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3718
BstXI CCAn_nnnn'nTGG
Cuts at: 547 547
Size: 3718
BstYI r'GATC_y
Cuts at: 1 536 741 1208 1231 1566 1812 2826 2912
Size: 535 205 467 23 335 246 1014 86
Cuts at: 2912 2924 3270 1
Size: 12 346 449
Fragments arranged by size:
1014 535 467 449 346 335 246 205
86 23 12
BtgI C'CryG_G
Cuts at: 125 656 125
Size: 531 3187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3374
Fragments arranged by size:
3374 181 163
BtsI GCAGTG_nn'
Cuts at: 552 959 552
Size: 407 3311
DraI TTT'AAA
Cuts at: 715 715
Size: 3718
DraIII CAC_nnn'GTG
Cuts at: 445 445
Size: 3718
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2282 1422
Size: 860 2858
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3240 1301
Size: 174 391 27 1347 1779
Fragments arranged by size:
1779 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3718
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 574 1924 2236 2382 3528 574
Size: 1350 312 146 1146 764
Fragments arranged by size:
1350 1146 764 312 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 535 613 1541 1973 2722 2990 293
Size: 242 78 928 432 749 268 1021
Fragments arranged by size:
1021 928 749 432 268 242 78
EcoNI CCTnn'n_nnAGG
Cuts at: 595 595
Size: 3718
EcoO109I rG'GnC_Cy
Cuts at: 231 393 394 587 588 668 669 686 855
Size: 162 1 193 1 80 1 17 169
Cuts at: 855 231
Size: 3094
Fragments arranged by size:
3094 193 169 162 80 17 1 1
1
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3718
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3718
HaeII r_GCGC'y
Cuts at: 1150 1398 2422 3401 1150
Size: 248 1024 979 1467
Fragments arranged by size:
1467 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3656
Fragments arranged by size:
3656 30 30 2
HincII GTy'rAC
Cuts at: 459 755 459
Size: 296 3422
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3470
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3718
MluI A'CGCG_T
Cuts at: 3151 3151
Size: 3718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2389 2573 2389
Size: 184 3534
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3718
MslI CAynn'nnrTG
Cuts at: 545 1705 1832 545
Size: 1160 127 2431
Fragments arranged by size:
2431 1160 127
MspA1I CmG'CkG
Cuts at: 241 249 271 327 827 1141 1501 2516 2761
Size: 8 22 56 500 314 360 1015 245
Cuts at: 2761 241
Size: 1198
Fragments arranged by size:
1198 1015 500 360 314 245 56 22
8
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3718
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3470
NcoI C'CATG_G
Cuts at: 125 125
Size: 3718
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3718
NheI G'CTAG_C
Cuts at: 697 697
Size: 3718
NruI TCG'CGA
Cuts at: 2079 2079
Size: 3718
NspI r_CATG'y
Cuts at: 334 769 1269 2178 334
Size: 435 500 909 1874
Fragments arranged by size:
1874 909 500 435
PciI A'CATG_T
Cuts at: 2174 2174
Size: 3718
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2889 2857
Size: 32 3686
PsiI TTA'TAA
Cuts at: 185 185
Size: 3718
PspOMI G'GGCC_C
Cuts at: 231 393 587 668 231
Size: 162 194 81 3281
Fragments arranged by size:
3281 194 162 81
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3033
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3718
PvuII CAG'CTG
Cuts at: 241 271 327 1141 1501 241
Size: 30 56 814 360 2458
Fragments arranged by size:
2458 814 360 56 30
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3718
SacI G_AGCT'C
Cuts at: 730 730
Size: 3718
SalI G'TCGA_C
Cuts at: 753 753
Size: 3718
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3508
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3718
ScaI AGT'ACT
Cuts at: 3235 3235
Size: 3718
SfcI C'TryA_G
Cuts at: 33 680 759 1444 2439 2630 33
Size: 647 79 685 995 191 1121
Fragments arranged by size:
1121 995 685 647 191 79
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3470
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3718
SmaI CCC'GGG
Cuts at: 225 738 225
Size: 513 3205
SmlI C'TyrA_G
Cuts at: 871 1786 2280 2542 871
Size: 915 494 262 2047
Fragments arranged by size:
2047 915 494 262
SnaBI TAC'GTA
Cuts at: 708 708
Size: 3718
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3718
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3218
StyI C'CwwG_G
Cuts at: 125 850 1204 125
Size: 725 354 2639
Fragments arranged by size:
2639 725 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 379 1299 1432 1891 2102 379
Size: 920 133 459 211 1995
Fragments arranged by size:
1995 920 459 211 133
TatI w'GTAC_w
Cuts at: 3233 3233
Size: 3718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3235 3267 3235
Size: 32 3686
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3718
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3718
XmaI C'CCGG_G
Cuts at: 223 736 223
Size: 513 3205
XmnI GAAnn'nnTTC
Cuts at: 383 383
Size: 3718
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PciI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI