Restriction map for
pF1KSDA0272
(Circular) (Six-base) MAPSORT of: pF1KSDA0272.seq Check: 3232 from: 1 to: 5326
pF1KSDA0272 5326 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1252 1252
Size: 5326
AccI GT'mk_AC
Cuts at: 689 2362 689
Size: 1673 3653
Acc65I G'GTAC_C
Cuts at: 2340 2340
Size: 5326
AclI AA'CG_TT
Cuts at: 5166 5166
Size: 5326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 703 889 2053 3149 3581 4330 4598 214
Size: 489 186 1164 1096 432 749 268 942
Fragments arranged by size:
1164 1096 942 749 489 432 268 186
AflIII A'CryG_T
Cuts at: 362 2676 3782 4759 362
Size: 2314 1106 977 929
Fragments arranged by size:
2314 1106 977 929
AgeI A'CCGG_T
Cuts at: 3714 3714
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1822 1822
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 909 1039 1194 2629 4198 909
Size: 130 155 1435 1569 2037
Fragments arranged by size:
2037 1569 1435 155 130
ApaI G_GGCC'C
Cuts at: 250 710 1029 250
Size: 460 319 4547
Fragments arranged by size:
4547 460 319
ApaLI G'TGCA_C
Cuts at: 4096 4096
Size: 5326
ApoI r'AATT_y
Cuts at: 2328 4679 4935 2328
Size: 2351 256 2719
Fragments arranged by size:
2719 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5326
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5326
AvaI C'yCGr_G
Cuts at: 565 2344 565
Size: 1779 3547
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 320 353 320
Size: 33 5293
BamHI G'GATC_C
Cuts at: 2349 2349
Size: 5326
BanI G'GyrC_C
Cuts at: 825 2225 2340 2754 3002 3037 825
Size: 1400 115 414 248 35 3114
Fragments arranged by size:
3114 1400 414 248 115 35
BanII G_rGCy'C
Cuts at: 250 710 791 1029 1988 2338 3368 250
Size: 460 81 238 959 350 1030 2208
Fragments arranged by size:
2208 1030 959 460 350 238 81
BbeI G_GCGC'C
Cuts at: 2229 2758 3006 2229
Size: 529 248 4549
Fragments arranged by size:
4549 529 248
BbsI GAAGACnn'nnnn_
Cuts at: 1256 1966 1256
Size: 710 4616
BbvCI CC'TCA_GC
Cuts at: 1820 1820
Size: 5326
BciVI GTATCCnnnnn_n'
Cuts at: 3222 3985 5326 3222
Size: 763 1341 3222
Fragments arranged by size:
3222 1341 763
BclI T'GATC_A
Cuts at: 2844 2844
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 2274 2274
Size: 5326
BglII A'GATC_T
Cuts at: 218 2839 4878 218
Size: 2621 2039 666
Fragments arranged by size:
2621 2039 666
BlpI GC'TnA_GC
Cuts at: 415 1662 2436 415
Size: 1247 774 3305
Fragments arranged by size:
3305 1247 774
Bme1580I G_kGCm'C
Cuts at: 250 710 830 1029 1562 2949 3042 4100 250
Size: 460 120 199 533 1387 93 1058 1476
Fragments arranged by size:
1476 1387 1058 533 460 199 120 93
BmgBI CAC'GTC
Cuts at: 1350 1350
Size: 5326
BmrI ACTGGGnnnn_n'
Cuts at: 1912 2536 2617 2952 5098 1912
Size: 624 81 335 2146 2140
Fragments arranged by size:
2146 2140 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2022 3691 2022
Size: 1669 3657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 169 1124 1668 163
Size: 6 955 544 3821
Fragments arranged by size:
3821 955 544 6
Bpu10I CC'TnA_GC
Cuts at: 1820 2174 1820
Size: 354 4972
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 2010 2059 2500 3379 3873 4171 938
Size: 1072 49 441 879 494 298 2093
Fragments arranged by size:
2093 1072 879 494 441 298 49
BsaI GGTCTCn'nnnn_
Cuts at: 315 5030 315
Size: 4715 611
BsaAI yAC'GTr
Cuts at: 2316 2677 2316
Size: 361 4965
BsaBI GATnn'nnATC
Cuts at: 6 2838 4877 6
Size: 2832 2039 455
Fragments arranged by size:
2832 2039 455
BsaHI Gr'CG_yC
Cuts at: 2226 2755 3003 3707 5203 2226
Size: 529 248 704 1496 2349
Fragments arranged by size:
2349 1496 704 529 248
BsaWI w'CCGG_w
Cuts at: 2513 3034 3714 3988 4135 4546 2513
Size: 521 680 274 147 411 3293
Fragments arranged by size:
3293 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 563 593 967 997 563
Size: 30 374 30 4892
Fragments arranged by size:
4892 374 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 739 988 1081 1354 2095 229
Size: 510 249 93 273 741 3460
Fragments arranged by size:
3460 741 510 273 249 93
BseYI C'CCAG_C
Cuts at: 1298 1537 1588 2749 3507 4086 4582 1298
Size: 239 51 1161 758 579 496 2042
Fragments arranged by size:
2042 1161 758 579 496 239 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 1082 1372 1795 2136 626
Size: 456 290 423 341 3816
Fragments arranged by size:
3816 456 423 341 290
BsiEI CG_ry'CG
Cuts at: 104 834 2247 2866 2912 4122 104
Size: 730 1413 619 46 1210 1308
Fragments arranged by size:
1413 1308 1210 730 619 46
BsiHKAI G_wGCw'C
Cuts at: 425 2338 3116 3306 4100 425
Size: 1913 778 190 794 1651
Fragments arranged by size:
1913 1651 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 250 425 710 791 830 1029 1562 1988 2338
Size: 175 285 81 39 199 533 426 350
Cuts at: 2338 2949 3042 3116 3306 3368 4100 250
Size: 611 93 74 190 62 732 1476
Fragments arranged by size:
1476 732 611 533 426 350 285 199
190 175 93 81 74 62 39
BspEI T'CCGG_A
Cuts at: 4546 4546
Size: 5326
BspHI T'CATG_A
Cuts at: 4502 4502
Size: 5326
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1252 2374 2588 2890 3271 1252
Size: 1122 214 302 381 3307
Fragments arranged by size:
3307 1122 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1415 1661 3616 3670 5156 5321 50
Size: 1365 246 1955 54 1486 165 55
Fragments arranged by size:
1955 1486 1365 246 165 55 54
BsrDI GCAATG_nn'
Cuts at: 528 1599 3236 528
Size: 1071 1637 2618
Fragments arranged by size:
2618 1637 1071
BsrFI r'CCGG_y
Cuts at: 293 659 1197 2246 2273 3322 3503 3714 293
Size: 366 538 1049 27 1049 181 211 1905
Fragments arranged by size:
1905 1049 1049 538 366 211 181 27
BssSI C'ACGA_G
Cuts at: 178 3595 3955 178
Size: 3417 360 1549
Fragments arranged by size:
3417 1549 360
BstAPI GCAn_nnn'nTGC
Cuts at: 406 2701 406
Size: 2295 3031
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5326
BstXI CCAn_nnnn'nTGG
Cuts at: 1057 1128 2199 1057
Size: 71 1071 4184
Fragments arranged by size:
4184 1071 71
BstYI r'GATC_y
Cuts at: 1 218 2349 2816 2839 3174 3420 4434 4520
Size: 217 2131 467 23 335 246 1014 86
Cuts at: 4520 4532 4878 1
Size: 12 346 449
Fragments arranged by size:
2131 1014 467 449 346 335 246 217
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 971 1372 971
Size: 401 4925
BtgI C'CryG_G
Cuts at: 617 702 1943 617
Size: 85 1241 4000
Fragments arranged by size:
4000 1241 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3274 3455 3618 3274
Size: 181 163 4982
Fragments arranged by size:
4982 181 163
BtsI GCAGTG_nn'
Cuts at: 614 776 827 1562 1632 1642 2026 2567 614
Size: 162 51 735 70 10 384 541 3373
Fragments arranged by size:
3373 735 541 384 162 70 51 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1164 1199 1164
Size: 35 5291
DraI TTT'AAA
Cuts at: 2323 2323
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 948 3030 3890 948
Size: 2082 860 2384
Fragments arranged by size:
2384 2082 860
EaeI y'GGCC_r
Cuts at: 536 2189 2244 2909 3083 3474 3501 4848 536
Size: 1653 55 665 174 391 27 1347 1014
Fragments arranged by size:
1653 1347 1014 665 391 174 55 27
EagI C'GGCC_G
Cuts at: 2244 2909 2244
Size: 665 4661
EarI CTCTTCn'nnn_
Cuts at: 172 281 2086 3347 3557 172
Size: 109 1805 1261 210 1941
Fragments arranged by size:
1941 1805 1261 210 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1279 2292 3532 3844 3990 5136 1279
Size: 1013 1240 312 146 1146 1469
Fragments arranged by size:
1469 1240 1146 1013 312 146
EcoICRI GAG'CTC
Cuts at: 2336 2336
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 169 214 703 889 1124 1668 2053 3149
Size: 6 45 489 186 235 544 385 1096
Cuts at: 3149 3581 4330 4598 163
Size: 432 749 268 891
Fragments arranged by size:
1096 891 749 544 489 432 385 268
235 186 45 6
EcoNI CCTnn'n_nnAGG
Cuts at: 653 1819 2077 653
Size: 1166 258 3902
Fragments arranged by size:
3902 1166 258
EcoO109I rG'GnC_Cy
Cuts at: 246 247 451 707 1025 1026 1497 1786 2126
Size: 1 204 256 318 1 471 289 340
Cuts at: 2126 2463 246
Size: 337 3109
Fragments arranged by size:
3109 471 340 337 318 289 256 204
1 1
EcoRI G'AATT_C
Cuts at: 2328 2328
Size: 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3343 3343
Size: 5326
FspI TGC'GCA
Cuts at: 1086 3105 1086
Size: 2019 3307
FspAI rTGC'GCAy
Cuts at: 1086 1086
Size: 5326
HaeII r_GCGC'y
Cuts at: 294 1849 2229 2310 2758 3006 4030 5009 294
Size: 1555 380 81 448 248 1024 979 611
Fragments arranged by size:
1555 1024 979 611 448 380 248 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 280 312 316 348 967 999 1271
Size: 32 23 32 4 32 619 32 272
Cuts at: 1271 1303 1597 1629 3166 3196 3198 3228 225
Size: 32 294 32 1537 30 2 30 2323
Fragments arranged by size:
2323 1537 619 294 272 32 32 32
32 32 32 30 30 23 4 2
HincII GTy'rAC
Cuts at: 1749 2363 1749
Size: 614 4712
KasI G'GCGC_C
Cuts at: 2225 2754 3002 2225
Size: 529 248 4549
Fragments arranged by size:
4549 529 248
KpnI G_GTAC'C
Cuts at: 2344 2344
Size: 5326
MluI A'CGCG_T
Cuts at: 4759 4759
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4181 3997
Size: 184 5142
MscI TGG'CCA
Cuts at: 538 2191 3085 538
Size: 1653 894 2779
Fragments arranged by size:
2779 1653 894
MslI CAynn'nnrTG
Cuts at: 1055 1220 2215 3313 3440 1055
Size: 165 995 1098 127 2941
Fragments arranged by size:
2941 1098 995 165 127
MspA1I CmG'CkG
Cuts at: 381 909 966 1005 1191 1532 2262 2435 2749
Size: 528 57 39 186 341 730 173 314
Cuts at: 2749 3109 4124 4369 381
Size: 360 1015 245 1338
Fragments arranged by size:
1338 1015 730 528 360 341 314 245
186 173 57 39
NaeI GCC'GGC
Cuts at: 661 1199 2248 3505 661
Size: 538 1049 1257 2482
Fragments arranged by size:
2482 1257 1049 538
NarI GG'CG_CC
Cuts at: 2226 2755 3003 2226
Size: 529 248 4549
Fragments arranged by size:
4549 529 248
NcoI C'CATG_G
Cuts at: 617 702 617
Size: 85 5241
NgoMIV G'CCGG_C
Cuts at: 659 1197 2246 3503 659
Size: 538 1049 1257 2482
Fragments arranged by size:
2482 1257 1049 538
NruI TCG'CGA
Cuts at: 3687 3687
Size: 5326
NspI r_CATG'y
Cuts at: 366 865 2187 2377 2877 3786 366
Size: 499 1322 190 500 909 1906
Fragments arranged by size:
1906 1322 909 500 499 190
PasI CC'CwG_GG
Cuts at: 710 710
Size: 5326
PciI A'CATG_T
Cuts at: 362 3782 362
Size: 3420 1906
PmeI GTTT'AAAC
Cuts at: 2323 2323
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 4465 4497 1348
Size: 32 3085 32 2177
Fragments arranged by size:
3085 2177 32 32
PpuMI rG'GwC_Cy
Cuts at: 451 1497 1786 2126 451
Size: 1046 289 340 3651
Fragments arranged by size:
3651 1046 340 289
PspOMI G'GGCC_C
Cuts at: 246 706 1025 246
Size: 460 319 4547
Fragments arranged by size:
4547 460 319
PstI C_TGCA'G
Cuts at: 436 783 905 1035 1448 2371 3056 436
Size: 347 122 130 413 923 685 2706
Fragments arranged by size:
2706 923 685 413 347 130 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5326
PvuII CAG'CTG
Cuts at: 381 909 966 2749 3109 381
Size: 528 57 1783 360 2598
Fragments arranged by size:
2598 1783 528 360 57
RsrII CG'GwC_CG
Cuts at: 3519 3519
Size: 5326
SacI G_AGCT'C
Cuts at: 2338 2338
Size: 5326
SalI G'TCGA_C
Cuts at: 2361 2361
Size: 5326
SanDI GG'GwC_CC
Cuts at: 451 451
Size: 5326
SapI GCTCTTCn'nnn_
Cuts at: 281 3347 3557 281
Size: 3066 210 2050
Fragments arranged by size:
3066 2050 210
SbfI CC_TGCA'GG
Cuts at: 2371 2371
Size: 5326
ScaI AGT'ACT
Cuts at: 890 1306 4843 890
Size: 416 3537 1373
Fragments arranged by size:
3537 1373 416
SfcI C'TryA_G
Cuts at: 33 432 779 901 1031 1444 2367 3052 4047
Size: 399 347 122 130 413 923 685 995
Cuts at: 4047 4238 33
Size: 191 1121
Fragments arranged by size:
1121 995 923 685 413 399 347 191
130 122
SfoI GGC'GCC
Cuts at: 2227 2756 3004 2227
Size: 529 248 4549
Fragments arranged by size:
4549 529 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5326
SmaI CCC'GGG
Cuts at: 2346 2346
Size: 5326
SmlI C'TyrA_G
Cuts at: 953 1989 2074 2479 3394 3888 4150 953
Size: 1036 85 405 915 494 262 2129
Fragments arranged by size:
2129 1036 915 494 405 262 85
SnaBI TAC'GTA
Cuts at: 2316 2316
Size: 5326
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5326
SphI G_CATG'C
Cuts at: 2187 2377 2877 2187
Size: 190 500 4636
Fragments arranged by size:
4636 500 190
StuI AGG'CCT
Cuts at: 164 1805 1977 164
Size: 1641 172 3513
Fragments arranged by size:
3513 1641 172
StyI C'CwwG_G
Cuts at: 317 482 539 617 702 1709 1893 2012 2229
Size: 165 57 78 85 1007 184 119 217
Cuts at: 2229 2458 2812 317
Size: 229 354 2831
Fragments arranged by size:
2831 1007 354 229 217 184 165 119
85 78 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 2025 2907 3040 3499 3710 1596
Size: 429 882 133 459 211 3212
Fragments arranged by size:
3212 882 459 429 211 133
TatI w'GTAC_w
Cuts at: 888 1304 1600 4841 888
Size: 416 296 3241 1373
Fragments arranged by size:
3241 1373 416 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 563 595 712 744 4843 4875 563
Size: 32 117 32 4099 32 1014
Fragments arranged by size:
4099 1014 117 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3121 3121
Size: 5326
XbaI T'CTAG_A
Cuts at: 1206 2355 1206
Size: 1149 4177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1464 1917 2019 2819 1464
Size: 453 102 800 3971
Fragments arranged by size:
3971 800 453 102
XmaI C'CCGG_G
Cuts at: 2344 2344
Size: 5326
XmnI GAAnn'nnTTC
Cuts at: 475 2101 475
Size: 1626 3700
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI