Restriction map for
pF1KSDA0273
(Circular) (Six-base) MAPSORT of: pF1KSDA0273.seq Check: 9379 from: 1 to: 4129
pF1KSDA0273 4129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 181 181
Size: 4129
AatII G_ACGT'C
Cuts at: 296 296
Size: 4129
AccI GT'mk_AC
Cuts at: 1001 1057 1165 1001
Size: 56 108 3965
Fragments arranged by size:
3965 108 56
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4129
AclI AA'CG_TT
Cuts at: 3969 3969
Size: 4129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 637 660 1952 2384 3133 3401 478
Size: 159 23 1292 432 749 268 1206
Fragments arranged by size:
1292 1206 749 432 268 159 23
AflIII A'CryG_T
Cuts at: 1479 2585 3562 1479
Size: 1106 977 2046
Fragments arranged by size:
2046 1106 977
AgeI A'CCGG_T
Cuts at: 2517 2517
Size: 4129
AlwNI CAG_nnn'CTG
Cuts at: 1432 3001 1432
Size: 1569 2560
ApaLI G'TGCA_C
Cuts at: 2899 2899
Size: 4129
ApoI r'AATT_y
Cuts at: 267 1131 3482 3738 267
Size: 864 2351 256 658
Fragments arranged by size:
2351 864 658 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4129
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4129
AvaI C'yCGr_G
Cuts at: 1147 1147
Size: 4129
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4129
BanI G'GyrC_C
Cuts at: 843 1015 1037 1143 1557 1805 1840 843
Size: 172 22 106 414 248 35 3132
Fragments arranged by size:
3132 414 248 172 106 35 22
BanII G_rGCy'C
Cuts at: 364 998 1141 2171 364
Size: 634 143 1030 2322
Fragments arranged by size:
2322 1030 634 143
BbeI G_GCGC'C
Cuts at: 847 1561 1809 847
Size: 714 248 3167
Fragments arranged by size:
3167 714 248
BbsI GAAGACnn'nnnn_
Cuts at: 534 663 942 534
Size: 129 279 3721
Fragments arranged by size:
3721 279 129
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2788 4129 2025
Size: 763 1341 2025
Fragments arranged by size:
2025 1341 763
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4129
BglII A'GATC_T
Cuts at: 1642 3681 1642
Size: 2039 2090
BlpI GC'TnA_GC
Cuts at: 488 506 755 1239 488
Size: 18 249 484 3378
Fragments arranged by size:
3378 484 249 18
Bme1580I G_kGCm'C
Cuts at: 1018 1040 1752 1845 2903 1018
Size: 22 712 93 1058 2244
Fragments arranged by size:
2244 1058 712 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 470 1339 1420 1755 3901 470
Size: 869 81 335 2146 698
Fragments arranged by size:
2146 869 698 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 456 997 238
Size: 218 541 3370
Fragments arranged by size:
3370 541 218
Bpu10I CC'TnA_GC
Cuts at: 358 358
Size: 4129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 2182 2676 2974 1303
Size: 879 494 298 2458
Fragments arranged by size:
2458 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3833 3833
Size: 4129
BsaAI yAC'GTr
Cuts at: 1119 1480 1119
Size: 361 3768
BsaBI GATnn'nnATC
Cuts at: 6 1641 3680 6
Size: 1635 2039 455
Fragments arranged by size:
2039 1635 455
BsaHI Gr'CG_yC
Cuts at: 293 844 1558 1806 2510 4006 293
Size: 551 714 248 704 1496 416
Fragments arranged by size:
1496 714 704 551 416 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2517 2791 2938 3349 1316
Size: 521 680 274 147 411 2096
Fragments arranged by size:
2096 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 121
Size: 30 4099
BseYI C'CCAG_C
Cuts at: 750 772 1081 1552 2310 2889 3385 750
Size: 22 309 471 758 579 496 1494
Fragments arranged by size:
1494 758 579 496 471 309 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 441 409
Size: 32 4097
BsiEI CG_ry'CG
Cuts at: 104 732 882 1669 1715 2925 104
Size: 628 150 787 46 1210 1308
Fragments arranged by size:
1308 1210 787 628 150 46
BsiHKAI G_wGCw'C
Cuts at: 482 1141 1919 2109 2903 482
Size: 659 778 190 794 1708
Fragments arranged by size:
1708 794 778 659 190
BsmBI CGTCTCn'nnnn_
Cuts at: 436 436
Size: 4129
Bsp1286I G_dGCh'C
Cuts at: 364 482 998 1018 1040 1141 1752 1845 1919
Size: 118 516 20 22 101 611 93 74
Cuts at: 1919 2109 2171 2903 364
Size: 190 62 732 1590
Fragments arranged by size:
1590 732 611 516 190 118 101 93
74 62 22 20
BspEI T'CCGG_A
Cuts at: 3349 3349
Size: 4129
BspHI T'CATG_A
Cuts at: 1046 3305 1046
Size: 2259 1870
BspMI ACCTGCnnnn'nnnn_
Cuts at: 181 931 1102 1177 1391 1693 2074 181
Size: 750 171 75 214 302 381 2236
Fragments arranged by size:
2236 750 381 302 214 171 75
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3959 4124 50
Size: 2369 54 1486 165 55
Fragments arranged by size:
2369 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 899 2039 899
Size: 1140 2989
BsrFI r'CCGG_y
Cuts at: 2125 2306 2517 2125
Size: 181 211 3737
Fragments arranged by size:
3737 211 181
BsrGI T'GTAC_A
Cuts at: 809 809
Size: 4129
BssSI C'ACGA_G
Cuts at: 307 2398 2758 307
Size: 2091 360 1678
Fragments arranged by size:
2091 1678 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1094 1504 1094
Size: 410 3719
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4129
BstEII G'GTnAC_C
Cuts at: 225 225
Size: 4129
BstXI CCAn_nnnn'nTGG
Cuts at: 309 309
Size: 4129
BstYI r'GATC_y
Cuts at: 1 746 1152 1619 1642 1977 2223 3237 3323
Size: 745 406 467 23 335 246 1014 86
Cuts at: 3323 3335 3681 1
Size: 12 346 449
Fragments arranged by size:
1014 745 467 449 406 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 200 636 663 125
Size: 75 436 27 3591
Fragments arranged by size:
3591 436 75 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 425 2077 2258 2421 425
Size: 1652 181 163 2133
Fragments arranged by size:
2133 1652 181 163
BtsI GCAGTG_nn'
Cuts at: 325 326 404 588 1370 325
Size: 1 78 184 782 3084
Fragments arranged by size:
3084 782 184 78 1
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4129
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2693 1833
Size: 860 3269
EaeI y'GGCC_r
Cuts at: 311 383 729 1712 1886 2277 2304 3651 311
Size: 72 346 983 174 391 27 1347 789
Fragments arranged by size:
1347 983 789 391 346 174 72 27
EagI C'GGCC_G
Cuts at: 729 1712 729
Size: 983 3146
EarI CTCTTCn'nnn_
Cuts at: 769 2150 2360 769
Size: 1381 210 2538
Fragments arranged by size:
2538 1381 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 943 2335 2647 2793 3939 943
Size: 1392 312 146 1146 1133
Fragments arranged by size:
1392 1146 1133 312 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 456 478 637 660 997 1952 2384 3133
Size: 218 22 159 23 337 955 432 749
Cuts at: 3133 3401 238
Size: 268 966
Fragments arranged by size:
966 955 749 432 337 268 218 159
23 22
EcoO109I rG'GnC_Cy
Cuts at: 584 966 1077 1266 584
Size: 382 111 189 3447
Fragments arranged by size:
3447 382 189 111
EcoRI G'AATT_C
Cuts at: 267 1131 267
Size: 864 3265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 2146 585
Size: 1561 2568
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4129
HaeII r_GCGC'y
Cuts at: 741 762 847 1561 1809 2833 3812 741
Size: 21 85 714 248 1024 979 1058
Fragments arranged by size:
1058 1024 979 714 248 85 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 738 770 1969 1999 2001 2031 121
Size: 32 585 32 1199 30 2 30 2219
Fragments arranged by size:
2219 1199 585 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1002 1166 1002
Size: 164 3965
KasI G'GCGC_C
Cuts at: 843 1557 1805 843
Size: 714 248 3167
Fragments arranged by size:
3167 714 248
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4129
MluI A'CGCG_T
Cuts at: 3562 3562
Size: 4129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 2800 2984 296
Size: 2504 184 1441
Fragments arranged by size:
2504 1441 184
MscI TGG'CCA
Cuts at: 313 1888 313
Size: 1575 2554
MslI CAynn'nnrTG
Cuts at: 337 835 1105 2116 2243 337
Size: 498 270 1011 127 2223
Fragments arranged by size:
2223 1011 498 270 127
MspA1I CmG'CkG
Cuts at: 629 653 680 734 1085 1238 1552 1912 2927
Size: 24 27 54 351 153 314 360 1015
Cuts at: 2927 3172 629
Size: 245 1586
Fragments arranged by size:
1586 1015 360 351 314 245 153 54
27 24
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4129
NarI GG'CG_CC
Cuts at: 844 1558 1806 844
Size: 714 248 3167
Fragments arranged by size:
3167 714 248
NcoI C'CATG_G
Cuts at: 125 200 125
Size: 75 4054
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4129
NruI TCG'CGA
Cuts at: 2490 2490
Size: 4129
NspI r_CATG'y
Cuts at: 528 580 603 810 1180 1680 2589 528
Size: 52 23 207 370 500 909 2068
Fragments arranged by size:
2068 909 500 370 207 52 23
PasI CC'CwG_GG
Cuts at: 858 1011 1020 858
Size: 153 9 3967
Fragments arranged by size:
3967 153 9
PciI A'CATG_T
Cuts at: 2585 2585
Size: 4129
PfoI T'CCnGG_A
Cuts at: 893 974 893
Size: 81 4048
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3268 3300 3268
Size: 32 4097
PpuMI rG'GwC_Cy
Cuts at: 584 966 1077 584
Size: 382 111 3636
Fragments arranged by size:
3636 382 111
PstI C_TGCA'G
Cuts at: 608 928 1174 1859 608
Size: 320 246 685 2878
Fragments arranged by size:
2878 685 320 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4129
PvuII CAG'CTG
Cuts at: 680 1085 1552 1912 680
Size: 405 467 360 2897
Fragments arranged by size:
2897 467 405 360
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4129
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4129
SalI G'TCGA_C
Cuts at: 1000 1164 1000
Size: 164 3965
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3919
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4129
ScaI AGT'ACT
Cuts at: 568 3646 568
Size: 3078 1051
SexAI A'CCwGG_T
Cuts at: 422 422
Size: 4129
SfcI C'TryA_G
Cuts at: 33 604 924 1170 1855 2850 3041 33
Size: 571 320 246 685 995 191 1121
Fragments arranged by size:
1121 995 685 571 320 246 191
SfoI GGC'GCC
Cuts at: 845 1559 1807 845
Size: 714 248 3167
Fragments arranged by size:
3167 714 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4129
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4129
SmlI C'TyrA_G
Cuts at: 1282 2197 2691 2953 1282
Size: 915 494 262 2458
Fragments arranged by size:
2458 915 494 262
SnaBI TAC'GTA
Cuts at: 1119 1119
Size: 4129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4129
SphI G_CATG'C
Cuts at: 528 580 1180 1680 528
Size: 52 600 500 2977
Fragments arranged by size:
2977 600 500 52
StyI C'CwwG_G
Cuts at: 125 200 314 473 1261 1615 125
Size: 75 114 159 788 354 2639
Fragments arranged by size:
2639 788 354 159 114 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 244 1710 1843 2302 2513 244
Size: 1466 133 459 211 1860
Fragments arranged by size:
1860 1466 459 211 133
TatI w'GTAC_w
Cuts at: 374 566 809 3644 374
Size: 192 243 2835 859
Fragments arranged by size:
2835 859 243 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3646 3678 3646
Size: 32 4097
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4129
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 480 837 855 1622 306
Size: 174 357 18 767 2813
Fragments arranged by size:
2813 767 357 174 18
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4129
ZraI GAC'GTC
Cuts at: 294 294
Size: 4129
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI