Restriction map for
pF1KSDA0275
(Circular) (Six-base) MAPSORT of: pF1KSDA0275.seq Check: 9853 from: 1 to: 4411
pF1KSDA0275 4411 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4411
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4411
AclI AA'CG_TT
Cuts at: 4251 4251
Size: 4411
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3415 3683 2234
Size: 432 749 268 2962
Fragments arranged by size:
2962 749 432 268
AflIII A'CryG_T
Cuts at: 1761 2867 3844 1761
Size: 1106 977 2328
Fragments arranged by size:
2328 1106 977
AgeI A'CCGG_T
Cuts at: 724 2799 724
Size: 2075 2336
AlwNI CAG_nnn'CTG
Cuts at: 735 1714 3283 735
Size: 979 1569 1863
Fragments arranged by size:
1863 1569 979
ApaI G_GGCC'C
Cuts at: 661 661
Size: 4411
ApaLI G'TGCA_C
Cuts at: 3181 3181
Size: 4411
ApoI r'AATT_y
Cuts at: 1413 3764 4020 1413
Size: 2351 256 1804
Fragments arranged by size:
2351 1804 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4411
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4411
AvaI C'yCGr_G
Cuts at: 135 1128 1272 1429 135
Size: 993 144 157 3117
Fragments arranged by size:
3117 993 157 144
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4411
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3714
Fragments arranged by size:
3714 414 248 35
BanII G_rGCy'C
Cuts at: 212 661 1246 1423 2453 212
Size: 449 585 177 1030 2170
Fragments arranged by size:
2170 1030 585 449 177
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4163
BciVI GTATCCnnnnn_n'
Cuts at: 369 2307 3070 4411 369
Size: 1938 763 1341 369
Fragments arranged by size:
1938 1341 763 369
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4411
BglI GCCn_nnn'nGGC
Cuts at: 826 826
Size: 4411
BglII A'GATC_T
Cuts at: 1924 3963 1924
Size: 2039 2372
BlpI GC'TnA_GC
Cuts at: 804 1521 804
Size: 717 3694
Bme1580I G_kGCm'C
Cuts at: 661 2034 2127 3185 661
Size: 1373 93 1058 1887
Fragments arranged by size:
1887 1373 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1621 1702 2037 4183 1621
Size: 81 335 2146 1849
Fragments arranged by size:
2146 1849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 628 1065 1096 1234 496
Size: 132 437 31 138 3673
Fragments arranged by size:
3673 437 138 132 31
Bpu10I CC'TnA_GC
Cuts at: 625 625
Size: 4411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1585 2464 2958 3256 196
Size: 1389 879 494 298 1351
Fragments arranged by size:
1389 1351 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 1020 4115 217
Size: 803 3095 513
Fragments arranged by size:
3095 803 513
BsaAI yAC'GTr
Cuts at: 1401 1762 1401
Size: 361 4050
BsaBI GATnn'nnATC
Cuts at: 6 1923 3962 6
Size: 1917 2039 455
Fragments arranged by size:
2039 1917 455
BsaHI Gr'CG_yC
Cuts at: 200 1840 2088 2792 4288 200
Size: 1640 248 704 1496 323
Fragments arranged by size:
1640 1496 704 323 248
BsaWI w'CCGG_w
Cuts at: 724 1154 1598 2119 2799 3073 3220 3631 724
Size: 430 444 521 680 274 147 411 1504
Fragments arranged by size:
1504 680 521 444 430 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 508 538 508
Size: 30 4381
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1324 1327 1354 1363 1366 1369 1324
Size: 3 27 9 3 3 4366
Fragments arranged by size:
4366 27 9 3 3 3
BseYI C'CCAG_C
Cuts at: 341 551 1206 1298 1834 2592 3171 3667 341
Size: 210 655 92 536 758 579 496 1085
Fragments arranged by size:
1085 758 655 579 536 496 210 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 429
Size: 4411
BsiEI CG_ry'CG
Cuts at: 104 500 1951 1997 3207 104
Size: 396 1451 46 1210 1308
Fragments arranged by size:
1451 1308 1210 396 46
BsiHKAI G_wGCw'C
Cuts at: 482 1423 2201 2391 3185 482
Size: 941 778 190 794 1708
Fragments arranged by size:
1708 941 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 305 1315 305
Size: 1010 3401
Bsp1286I G_dGCh'C
Cuts at: 212 482 661 1246 1423 2034 2127 2201 2391
Size: 270 179 585 177 611 93 74 190
Cuts at: 2391 2453 3185 212
Size: 62 732 1438
Fragments arranged by size:
1438 732 611 585 270 190 179 177
93 74 62
BspEI T'CCGG_A
Cuts at: 3631 3631
Size: 4411
BspHI T'CATG_A
Cuts at: 780 3587 780
Size: 2807 1604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1459 1673 1975 2356 1459
Size: 214 302 381 3514
Fragments arranged by size:
3514 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4241 4406 50
Size: 2651 54 1486 165 55
Fragments arranged by size:
2651 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 259 428 2321 259
Size: 169 1893 2349
Fragments arranged by size:
2349 1893 169
BsrFI r'CCGG_y
Cuts at: 724 820 2407 2588 2799 724
Size: 96 1587 181 211 2336
Fragments arranged by size:
2336 1587 211 181 96
BssHII G'CGCG_C
Cuts at: 129 129
Size: 4411
BssSI C'ACGA_G
Cuts at: 2680 3040 2680
Size: 360 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 539 1786 539
Size: 1247 3164
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4411
BstXI CCAn_nnnn'nTGG
Cuts at: 837 948 837
Size: 111 4300
BstYI r'GATC_y
Cuts at: 1 1088 1434 1901 1924 2259 2505 3519 3605
Size: 1087 346 467 23 335 246 1014 86
Cuts at: 3605 3617 3963 1
Size: 12 346 449
Fragments arranged by size:
1087 1014 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 176 510 674 176
Size: 334 164 3913
Fragments arranged by size:
3913 334 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 663 1268 2359 2540 2703 663
Size: 605 1091 181 163 2371
Fragments arranged by size:
2371 1091 605 181 163
BtsI GCAGTG_nn'
Cuts at: 819 1171 1652 819
Size: 352 481 3578
Fragments arranged by size:
3578 481 352
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4411
DraIII CAC_nnn'GTG
Cuts at: 423 423
Size: 4411
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2975 2115
Size: 860 3551
EaeI y'GGCC_r
Cuts at: 173 188 579 827 935 1008 1994 2168 2559
Size: 15 391 248 108 73 986 174 391
Cuts at: 2559 2586 3933 173
Size: 27 1347 651
Fragments arranged by size:
1347 986 651 391 391 248 174 108
73 27 15
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4411
EarI CTCTTCn'nnn_
Cuts at: 919 2432 2642 919
Size: 1513 210 2688
Fragments arranged by size:
2688 1513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2617 2929 3075 4221 2617
Size: 312 146 1146 2807
Fragments arranged by size:
2807 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1421 1421
Size: 4411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 628 1065 1096 1234 2234 2666 3415 3683
Size: 132 437 31 138 1000 432 749 268
Cuts at: 3683 496
Size: 1224
Fragments arranged by size:
1224 1000 749 437 432 268 138 132
31
EcoO109I rG'GnC_Cy
Cuts at: 387 657 658 732 1548 387
Size: 270 1 74 816 3250
Fragments arranged by size:
3250 816 270 74 1
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4411
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4411
HaeII r_GCGC'y
Cuts at: 1843 2091 3115 4094 1843
Size: 248 1024 979 2160
Fragments arranged by size:
2160 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2251 2281 2283 2313 2251
Size: 30 2 30 4349
Fragments arranged by size:
4349 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4411
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4163
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4411
MluI A'CGCG_T
Cuts at: 3844 3844
Size: 4411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3082 3266 3082
Size: 184 4227
MscI TGG'CCA
Cuts at: 581 2170 581
Size: 1589 2822
MslI CAynn'nnrTG
Cuts at: 2398 2525 2398
Size: 127 4284
MspA1I CmG'CkG
Cuts at: 162 178 281 447 639 833 860 1181 1206
Size: 16 103 166 192 194 27 321 25
Cuts at: 1206 1520 1834 2194 3209 3454 162
Size: 314 314 360 1015 245 1119
Fragments arranged by size:
1119 1015 360 321 314 314 245 194
192 166 103 27 25 16
NaeI GCC'GGC
Cuts at: 822 2590 822
Size: 1768 2643
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4163
NcoI C'CATG_G
Cuts at: 510 510
Size: 4411
NgoMIV G'CCGG_C
Cuts at: 820 2588 820
Size: 1768 2643
NruI TCG'CGA
Cuts at: 2772 2772
Size: 4411
NspI r_CATG'y
Cuts at: 1462 1962 2871 1462
Size: 500 909 3002
Fragments arranged by size:
3002 909 500
PasI CC'CwG_GG
Cuts at: 435 435
Size: 4411
PciI A'CATG_T
Cuts at: 2867 2867
Size: 4411
PflMI CCAn_nnn'nTGG
Cuts at: 878 878
Size: 4411
PfoI T'CCnGG_A
Cuts at: 917 1091 917
Size: 174 4237
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3550 3582 3550
Size: 32 4379
PpuMI rG'GwC_Cy
Cuts at: 387 732 387
Size: 345 4066
PspOMI G'GGCC_C
Cuts at: 657 657
Size: 4411
PstI C_TGCA'G
Cuts at: 1456 2141 1456
Size: 685 3726
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4411
PvuII CAG'CTG
Cuts at: 639 860 1206 1834 2194 639
Size: 221 346 628 360 2856
Fragments arranged by size:
2856 628 360 346 221
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4411
SacI G_AGCT'C
Cuts at: 1423 1423
Size: 4411
SacII CC_GC'GG
Cuts at: 179 179
Size: 4411
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4411
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4201
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4411
ScaI AGT'ACT
Cuts at: 3928 3928
Size: 4411
SfcI C'TryA_G
Cuts at: 33 1452 2137 3132 3323 33
Size: 1419 685 995 191 1121
Fragments arranged by size:
1419 1121 995 685 191
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4163
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4411
SmaI CCC'GGG
Cuts at: 137 1431 137
Size: 1294 3117
SmlI C'TyrA_G
Cuts at: 211 1564 2479 2973 3235 211
Size: 1353 915 494 262 1387
Fragments arranged by size:
1387 1353 915 494 262
SnaBI TAC'GTA
Cuts at: 1401 1401
Size: 4411
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4411
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3911
StyI C'CwwG_G
Cuts at: 203 383 510 1543 1897 203
Size: 180 127 1033 354 2717
Fragments arranged by size:
2717 1033 354 180 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1992 2125 2584 2795 1992
Size: 133 459 211 3608
Fragments arranged by size:
3608 459 211 133
TatI w'GTAC_w
Cuts at: 275 3926 275
Size: 3651 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3928 3960 3928
Size: 32 4379
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4411
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4411
XmaI C'CCGG_G
Cuts at: 135 1429 135
Size: 1294 3117
XmnI GAAnn'nnTTC
Cuts at: 304 304
Size: 4411
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsmI BsrGI BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI ZraI