Restriction map for
pF1KSDA0276
(Circular) (Six-base) MAPSORT of: pF1KSDA0276.seq Check: 4348 from: 1 to: 4015
pF1KSDA0276 4015 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 366 1051 366
Size: 685 3330
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4015
AclI AA'CG_TT
Cuts at: 3855 3855
Size: 4015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 434 512 1838 2270 3019 3287 269
Size: 165 78 1326 432 749 268 997
Fragments arranged by size:
1326 997 749 432 268 165 78
AflII C'TTAA_G
Cuts at: 672 672
Size: 4015
AflIII A'CryG_T
Cuts at: 1365 2471 3448 1365
Size: 1106 977 1932
Fragments arranged by size:
1932 1106 977
AgeI A'CCGG_T
Cuts at: 2403 2403
Size: 4015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 152 152
Size: 4015
AlwNI CAG_nnn'CTG
Cuts at: 472 1318 2887 472
Size: 846 1569 1600
Fragments arranged by size:
1600 1569 846
ApaLI G'TGCA_C
Cuts at: 2785 2785
Size: 4015
ApoI r'AATT_y
Cuts at: 505 1017 3368 3624 505
Size: 512 2351 256 896
Fragments arranged by size:
2351 896 512 256
AseI AT'TA_AT
Cuts at: 20 866 911 20
Size: 846 45 3124
Fragments arranged by size:
3124 846 45
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4015
AvaI C'yCGr_G
Cuts at: 1033 1033
Size: 4015
BamHI G'GATC_C
Cuts at: 1038 1038
Size: 4015
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3318
Fragments arranged by size:
3318 414 248 35
BanII G_rGCy'C
Cuts at: 1027 2057 1027
Size: 1030 2985
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3767
BbsI GAAGACnn'nnnn_
Cuts at: 239 333 521 239
Size: 94 188 3733
Fragments arranged by size:
3733 188 94
BbvCI CC'TCA_GC
Cuts at: 922 922
Size: 4015
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2674 4015 1911
Size: 763 1341 1911
Fragments arranged by size:
1911 1341 763
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4015
BglI GCCn_nnn'nGGC
Cuts at: 494 494
Size: 4015
BglII A'GATC_T
Cuts at: 197 1528 3567 197
Size: 1331 2039 645
Fragments arranged by size:
2039 1331 645
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4015
Bme1580I G_kGCm'C
Cuts at: 1638 1731 2789 1638
Size: 93 1058 2864
Fragments arranged by size:
2864 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3787 1225
Size: 81 335 2146 1453
Fragments arranged by size:
2146 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 4015
Bpu10I CC'TnA_GC
Cuts at: 922 922
Size: 4015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2562 2860 1189
Size: 879 494 298 2344
Fragments arranged by size:
2344 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3719 3719
Size: 4015
BsaAI yAC'GTr
Cuts at: 1005 1366 1005
Size: 361 3654
BsaBI GATnn'nnATC
Cuts at: 6 1527 3566 6
Size: 1521 2039 455
Fragments arranged by size:
2039 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2396 3892 1444
Size: 248 704 1496 1567
Fragments arranged by size:
1567 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2403 2677 2824 3235 1202
Size: 521 680 274 147 411 1982
Fragments arranged by size:
1982 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1438 2196 2775 3271 1438
Size: 758 579 496 2182
Fragments arranged by size:
2182 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2811 104
Size: 1451 46 1210 1308
Fragments arranged by size:
1451 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1027 1805 1995 2789 1027
Size: 778 190 794 2253
Fragments arranged by size:
2253 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 1027 1638 1731 1805 1995 2057 2789 1027
Size: 611 93 74 190 62 732 2253
Fragments arranged by size:
2253 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3235 3235
Size: 4015
BspHI T'CATG_A
Cuts at: 3191 3191
Size: 4015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 1063 1277 1579 1960 339
Size: 724 214 302 381 2394
Fragments arranged by size:
2394 724 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2305 2359 3845 4010 50
Size: 2255 54 1486 165 55
Fragments arranged by size:
2255 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 891 1925 891
Size: 1034 2981
BsrFI r'CCGG_y
Cuts at: 2011 2192 2403 2011
Size: 181 211 3623
Fragments arranged by size:
3623 211 181
BssSI C'ACGA_G
Cuts at: 2284 2644 2284
Size: 360 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4015
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4015
BstYI r'GATC_y
Cuts at: 1 197 1038 1505 1528 1863 2109 3123 3209
Size: 196 841 467 23 335 246 1014 86
Cuts at: 3209 3221 3567 1
Size: 12 346 449
Fragments arranged by size:
1014 841 467 449 346 335 246 196
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3671
Fragments arranged by size:
3671 181 163
BtsI GCAGTG_nn'
Cuts at: 770 1256 770
Size: 486 3529
DraI TTT'AAA
Cuts at: 705 1012 705
Size: 307 3708
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2579 1719
Size: 860 3155
EaeI y'GGCC_r
Cuts at: 240 949 1598 1772 2163 2190 3537 240
Size: 709 649 174 391 27 1347 718
Fragments arranged by size:
1347 718 709 649 391 174 27
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4015
EarI CTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3805
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2533 2679 3825 2221
Size: 312 146 1146 2411
Fragments arranged by size:
2411 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 359 434 512 1838 2270 3019 3287 269
Size: 90 75 78 1326 432 749 268 997
Fragments arranged by size:
1326 997 749 432 268 90 78 75
EcoO109I rG'GnC_Cy
Cuts at: 487 1152 487
Size: 665 3350
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4015
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4015
HaeII r_GCGC'y
Cuts at: 1447 1695 2719 3698 1447
Size: 248 1024 979 1764
Fragments arranged by size:
1764 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 334 366 1855 1885 1887 1917 334
Size: 32 1489 30 2 30 2432
Fragments arranged by size:
2432 1489 32 30 30 2
HincII GTy'rAC
Cuts at: 1052 1052
Size: 4015
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3767
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4015
MluI A'CGCG_T
Cuts at: 3448 3448
Size: 4015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 941 2686 2870 941
Size: 1745 184 2086
Fragments arranged by size:
2086 1745 184
MscI TGG'CCA
Cuts at: 242 951 1774 242
Size: 709 823 2483
Fragments arranged by size:
2483 823 709
MslI CAynn'nnrTG
Cuts at: 134 2002 2129 134
Size: 1868 127 2020
Fragments arranged by size:
2020 1868 127
MspA1I CmG'CkG
Cuts at: 145 159 1124 1438 1798 2813 3058 145
Size: 14 965 314 360 1015 245 1102
Fragments arranged by size:
1102 1015 965 360 314 245 14
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 4015
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3767
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 4015
NruI TCG'CGA
Cuts at: 2376 2376
Size: 4015
NspI r_CATG'y
Cuts at: 381 786 1066 1566 2475 381
Size: 405 280 500 909 1921
Fragments arranged by size:
1921 909 500 405 280
PciI A'CATG_T
Cuts at: 2471 2471
Size: 4015
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3186 3154
Size: 32 3983
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3330
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4015
PvuII CAG'CTG
Cuts at: 159 1438 1798 159
Size: 1279 360 2376
Fragments arranged by size:
2376 1279 360
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4015
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4015
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4015
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3805
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4015
ScaI AGT'ACT
Cuts at: 3532 3532
Size: 4015
SfcI C'TryA_G
Cuts at: 33 598 1056 1741 2736 2927 33
Size: 565 458 685 995 191 1121
Fragments arranged by size:
1121 995 685 565 458 191
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3767
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4015
SmaI CCC'GGG
Cuts at: 1035 1035
Size: 4015
SmlI C'TyrA_G
Cuts at: 672 1168 2083 2577 2839 672
Size: 496 915 494 262 1848
Fragments arranged by size:
1848 915 496 494 262
SnaBI TAC'GTA
Cuts at: 1005 1005
Size: 4015
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4015
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3515
SspI AAT'ATT
Cuts at: 189 189
Size: 4015
StyI C'CwwG_G
Cuts at: 313 1147 1501 313
Size: 834 354 2827
Fragments arranged by size:
2827 834 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1596 1729 2188 2399 1596
Size: 133 459 211 3212
Fragments arranged by size:
3212 459 211 133
TatI w'GTAC_w
Cuts at: 3530 3530
Size: 4015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3532 3564 3532
Size: 32 3983
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4015
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 1508 783
Size: 725 3290
XmaI C'CCGG_G
Cuts at: 1033 1033
Size: 4015
XmnI GAAnn'nnTTC
Cuts at: 621 621
Size: 4015
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI