Restriction map for
pF1KSDA0277
(Circular) (Six-base) MAPSORT of: pF1KSDA0277.seq Check: 4207 from: 1 to: 4879
pF1KSDA0277 4879 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1073 1473 1915 1073
Size: 400 442 4037
Fragments arranged by size:
4037 442 400
Acc65I G'GTAC_C
Cuts at: 1893 1893
Size: 4879
AclI AA'CG_TT
Cuts at: 4719 4719
Size: 4879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 1000 2702 3134 3883 4151 635
Size: 365 1702 432 749 268 1363
Fragments arranged by size:
1702 1363 749 432 365 268
AflII C'TTAA_G
Cuts at: 802 802
Size: 4879
AflIII A'CryG_T
Cuts at: 863 2229 3335 4312 863
Size: 1366 1106 977 1430
Fragments arranged by size:
1430 1366 1106 977
AgeI A'CCGG_T
Cuts at: 3267 3267
Size: 4879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 1381 521
Size: 860 4019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 264
Size: 32 4847
AlwNI CAG_nnn'CTG
Cuts at: 489 1714 1830 2182 3751 489
Size: 1225 116 352 1569 1617
Fragments arranged by size:
1617 1569 1225 352 116
ApaLI G'TGCA_C
Cuts at: 3649 3649
Size: 4879
ApoI r'AATT_y
Cuts at: 709 851 1188 1381 1425 1881 4232 4488 709
Size: 142 337 193 44 456 2351 256 1100
Fragments arranged by size:
2351 1100 456 337 256 193 142 44
AseI AT'TA_AT
Cuts at: 20 1183 20
Size: 1163 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4879
AvaI C'yCGr_G
Cuts at: 1048 1859 1897 1048
Size: 811 38 4030
Fragments arranged by size:
4030 811 38
BamHI G'GATC_C
Cuts at: 1902 1902
Size: 4879
BanI G'GyrC_C
Cuts at: 1893 2307 2555 2590 1893
Size: 414 248 35 4182
Fragments arranged by size:
4182 414 248 35
BanII G_rGCy'C
Cuts at: 1841 1891 2921 1841
Size: 50 1030 3799
Fragments arranged by size:
3799 1030 50
BbeI G_GCGC'C
Cuts at: 2311 2559 2311
Size: 248 4631
BbsI GAAGACnn'nnnn_
Cuts at: 281 450 281
Size: 169 4710
BbvCI CC'TCA_GC
Cuts at: 142 142
Size: 4879
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3538 4879 2775
Size: 763 1341 2775
Fragments arranged by size:
2775 1341 763
BclI T'GATC_A
Cuts at: 2397 2397
Size: 4879
BfrBI ATG'CAT
Cuts at: 1579 1579
Size: 4879
BglI GCCn_nnn'nGGC
Cuts at: 1355 1355
Size: 4879
BglII A'GATC_T
Cuts at: 2392 4431 2392
Size: 2039 2840
BlpI GC'TnA_GC
Cuts at: 1989 1989
Size: 4879
Bme1580I G_kGCm'C
Cuts at: 2502 2595 3653 2502
Size: 93 1058 3728
Fragments arranged by size:
3728 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 576 2089 2170 2505 4651 576
Size: 1513 81 335 2146 804
Fragments arranged by size:
2146 1513 804 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 552 3244 321
Size: 231 2692 1956
Fragments arranged by size:
2692 1956 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1279 802
Size: 477 4402
Bpu10I CC'TnA_GC
Cuts at: 142 142
Size: 4879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2932 3426 3724 2053
Size: 879 494 298 3208
Fragments arranged by size:
3208 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 1456 4583 415
Size: 1041 3127 711
Fragments arranged by size:
3127 1041 711
BsaAI yAC'GTr
Cuts at: 449 864 1869 2230 449
Size: 415 1005 361 3098
Fragments arranged by size:
3098 1005 415 361
BsaBI GATnn'nnATC
Cuts at: 6 2391 4430 6
Size: 2385 2039 455
Fragments arranged by size:
2385 2039 455
BsaHI Gr'CG_yC
Cuts at: 2308 2556 3260 4756 2308
Size: 248 704 1496 2431
Fragments arranged by size:
2431 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1076 2066 2587 3267 3541 3688 4099 1076
Size: 990 521 680 274 147 411 1856
Fragments arranged by size:
1856 990 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 264 294 264
Size: 30 4849
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 265
Size: 4879
BseYI C'CCAG_C
Cuts at: 1353 2302 3060 3639 4135 1353
Size: 949 758 579 496 2097
Fragments arranged by size:
2097 949 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 4879
BsiEI CG_ry'CG
Cuts at: 104 2419 2465 3675 104
Size: 2315 46 1210 1308
Fragments arranged by size:
2315 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 374 881 1841 1891 2669 2859 3653 374
Size: 507 960 50 778 190 794 1600
Fragments arranged by size:
1600 960 794 778 507 190 50
BsmI GAATG_Cn'
Cuts at: 734 1579 734
Size: 845 4034
Bsp1286I G_dGCh'C
Cuts at: 374 881 1841 1891 2502 2595 2669 2859 2921
Size: 507 960 50 611 93 74 190 62
Cuts at: 2921 3653 374
Size: 732 1600
Fragments arranged by size:
1600 960 732 611 507 190 93 74
62 50
BspEI T'CCGG_A
Cuts at: 4099 4099
Size: 4879
BspHI T'CATG_A
Cuts at: 461 1647 4055 461
Size: 1186 2408 1285
Fragments arranged by size:
2408 1285 1186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1927 2141 2443 2824 1927
Size: 214 302 381 3982
Fragments arranged by size:
3982 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3169 3223 4709 4874 50
Size: 3119 54 1486 165 55
Fragments arranged by size:
3119 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1311 2789 1311
Size: 1478 3401
BsrFI r'CCGG_y
Cuts at: 2875 3056 3267 2875
Size: 181 211 4487
Fragments arranged by size:
4487 211 181
BsrGI T'GTAC_A
Cuts at: 596 596
Size: 4879
BssSI C'ACGA_G
Cuts at: 1219 3148 3508 1219
Size: 1929 360 2590
Fragments arranged by size:
2590 1929 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 2254
Size: 4879
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4879
BstEII G'GTnAC_C
Cuts at: 608 1753 608
Size: 1145 3734
BstXI CCAn_nnnn'nTGG
Cuts at: 1446 1446
Size: 4879
BstYI r'GATC_y
Cuts at: 1 926 950 969 1167 1545 1902 2369 2392
Size: 925 24 19 198 378 357 467 23
Cuts at: 2392 2727 2973 3987 4073 4085 4431 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 925 467 449 378 357 346 335
246 198 86 24 23 19 12
BtgI C'CryG_G
Cuts at: 125 182 1328 125
Size: 57 1146 3676
Fragments arranged by size:
3676 1146 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2827 3008 3171 2827
Size: 181 163 4535
Fragments arranged by size:
4535 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1401 1457 1731 2120 1271
Size: 130 56 274 389 4030
Fragments arranged by size:
4030 389 274 130 56
DraI TTT'AAA
Cuts at: 641 1876 641
Size: 1235 3644
DrdI GACnn_nn'nnGTC
Cuts at: 2583 3443 2583
Size: 860 4019
EaeI y'GGCC_r
Cuts at: 983 1325 2462 2636 3027 3054 4401 983
Size: 342 1137 174 391 27 1347 1461
Fragments arranged by size:
1461 1347 1137 391 342 174 27
EagI C'GGCC_G
Cuts at: 2462 2462
Size: 4879
EarI CTCTTCn'nnn_
Cuts at: 959 2900 3110 959
Size: 1941 210 2728
Fragments arranged by size:
2728 1941 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 1105 3085 3397 3543 4689 168
Size: 937 1980 312 146 1146 358
Fragments arranged by size:
1980 1146 937 358 312 146
EcoICRI GAG'CTC
Cuts at: 1839 1889 1839
Size: 50 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 802 1000 1279 2702 3134 3883 4151 635
Size: 167 198 279 1423 432 749 268 1363
Fragments arranged by size:
1423 1363 749 432 279 268 198 167
EcoO109I rG'GnC_Cy
Cuts at: 350 2016 350
Size: 1666 3213
EcoRI G'AATT_C
Cuts at: 1425 1881 1425
Size: 456 4423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4879
FspI TGC'GCA
Cuts at: 2658 2658
Size: 4879
HaeII r_GCGC'y
Cuts at: 190 2311 2559 3583 4562 190
Size: 2121 248 1024 979 507
Fragments arranged by size:
2121 1024 979 507 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1771 1803 1842 1874 2719 2749 2751 2781 1771
Size: 32 39 32 845 30 2 30 3869
Fragments arranged by size:
3869 845 39 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1474 1916 1474
Size: 442 4437
KasI G'GCGC_C
Cuts at: 2307 2555 2307
Size: 248 4631
KpnI G_GTAC'C
Cuts at: 1897 1897
Size: 4879
MluI A'CGCG_T
Cuts at: 4312 4312
Size: 4879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3550 3734 3550
Size: 184 4695
MscI TGG'CCA
Cuts at: 985 1327 2638 985
Size: 342 1311 3226
Fragments arranged by size:
3226 1311 342
MslI CAynn'nnrTG
Cuts at: 884 1444 1805 2866 2993 884
Size: 560 361 1061 127 2770
Fragments arranged by size:
2770 1061 560 361 127
MspA1I CmG'CkG
Cuts at: 184 1353 1371 1988 2302 2662 3677 3922 184
Size: 1169 18 617 314 360 1015 245 1141
Fragments arranged by size:
1169 1141 1015 617 360 314 245 18
NaeI GCC'GGC
Cuts at: 3058 3058
Size: 4879
NarI GG'CG_CC
Cuts at: 2308 2556 2308
Size: 248 4631
NcoI C'CATG_G
Cuts at: 125 125
Size: 4879
NdeI CA'TA_TG
Cuts at: 1700 1700
Size: 4879
NgoMIV G'CCGG_C
Cuts at: 3056 3056
Size: 4879
NruI TCG'CGA
Cuts at: 3240 3240
Size: 4879
NsiI A_TGCA'T
Cuts at: 1581 1581
Size: 4879
NspI r_CATG'y
Cuts at: 1930 2430 3339 1930
Size: 500 909 3470
Fragments arranged by size:
3470 909 500
PasI CC'CwG_GG
Cuts at: 1501 1501
Size: 4879
PciI A'CATG_T
Cuts at: 3335 3335
Size: 4879
PfoI T'CCnGG_A
Cuts at: 346 404 428 346
Size: 58 24 4797
Fragments arranged by size:
4797 58 24
PmeI GTTT'AAAC
Cuts at: 1876 1876
Size: 4879
PmlI CAC'GTG
Cuts at: 864 864
Size: 4879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 264 296 4018 4050 264
Size: 32 3722 32 1093
Fragments arranged by size:
3722 1093 32 32
PpuMI rG'GwC_Cy
Cuts at: 350 350
Size: 4879
PspXI vC'TCGA_Gb
Cuts at: 1048 1048
Size: 4879
PstI C_TGCA'G
Cuts at: 1348 1738 1924 2609 1348
Size: 390 186 685 3618
Fragments arranged by size:
3618 685 390 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4879
PvuII CAG'CTG
Cuts at: 1353 1371 2302 2662 1353
Size: 18 931 360 3570
Fragments arranged by size:
3570 931 360 18
RsrII CG'GwC_CG
Cuts at: 3072 3072
Size: 4879
SacI G_AGCT'C
Cuts at: 1841 1891 1841
Size: 50 4829
SacII CC_GC'GG
Cuts at: 185 185
Size: 4879
SalI G'TCGA_C
Cuts at: 1472 1914 1472
Size: 442 4437
SanDI GG'GwC_CC
Cuts at: 350 350
Size: 4879
SapI GCTCTTCn'nnn_
Cuts at: 2900 3110 2900
Size: 210 4669
SbfI CC_TGCA'GG
Cuts at: 1924 1924
Size: 4879
ScaI AGT'ACT
Cuts at: 4396 4396
Size: 4879
SfcI C'TryA_G
Cuts at: 33 746 1344 1569 1734 1920 2605 3600 3791
Size: 713 598 225 165 186 685 995 191
Cuts at: 3791 33
Size: 1121
Fragments arranged by size:
1121 995 713 685 598 225 191 186
165
SfoI GGC'GCC
Cuts at: 2309 2557 2309
Size: 248 4631
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4879
SmaI CCC'GGG
Cuts at: 1899 1899
Size: 4879
SmlI C'TyrA_G
Cuts at: 802 1048 2032 2947 3441 3703 802
Size: 246 984 915 494 262 1978
Fragments arranged by size:
1978 984 915 494 262 246
SnaBI TAC'GTA
Cuts at: 1869 1869
Size: 4879
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4879
SphI G_CATG'C
Cuts at: 1930 2430 1930
Size: 500 4379
SspI AAT'ATT
Cuts at: 757 757
Size: 4879
StyI C'CwwG_G
Cuts at: 125 2011 2365 125
Size: 1886 354 2639
Fragments arranged by size:
2639 1886 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 212 1311 2460 2593 3052 3263 212
Size: 1099 1149 133 459 211 1828
Fragments arranged by size:
1828 1149 1099 459 211 133
TatI w'GTAC_w
Cuts at: 596 660 4394 596
Size: 64 3734 1081
Fragments arranged by size:
3734 1081 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1589 1621 4396 4428 1589
Size: 32 2775 32 2040
Fragments arranged by size:
2775 2040 32 32
Tth111I GACn'n_nGTC
Cuts at: 1030 1714 2674 1030
Size: 684 960 3235
Fragments arranged by size:
3235 960 684
XbaI T'CTAG_A
Cuts at: 1908 1908
Size: 4879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2372
Size: 4879
XhoI C'TCGA_G
Cuts at: 1048 1048
Size: 4879
XmaI C'CCGG_G
Cuts at: 1897 1897
Size: 4879
XmnI GAAnn'nnTTC
Cuts at: 1381 1429 1519 1381
Size: 48 90 4741
Fragments arranged by size:
4741 90 48
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BcgI BmgBI BmtI BsiWI BsmBI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI PacI PflMI PshAI
PsiI PspOMI PsrI SexAI SfiI SgrAI SrfI StuI
SwaI ZraI