Restriction map for
pF1KSDA0283
(Circular) (Six-base) MAPSORT of: pF1KSDA0283.seq Check: 7836 from: 1 to: 7460
pF1KSDA0283 7460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2334 2334
Size: 7460
AatII G_ACGT'C
Cuts at: 2606 2606
Size: 7460
AccI GT'mk_AC
Cuts at: 491 1145 1313 3146 4415 4498 491
Size: 654 168 1833 1269 83 3453
Fragments arranged by size:
3453 1833 1269 654 168 83
Acc65I G'GTAC_C
Cuts at: 4476 4476
Size: 7460
AclI AA'CG_TT
Cuts at: 7300 7300
Size: 7460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 1111 2008 5285 5717 6464 6732 430
Size: 681 897 3277 432 747 268 1158
Fragments arranged by size:
3277 1158 897 747 681 432 268
AfeI AGC'GCT
Cuts at: 4177 4177
Size: 7460
AflIII A'CryG_T
Cuts at: 4340 4812 5916 6893 4340
Size: 472 1104 977 4907
Fragments arranged by size:
4907 1104 977 472
AgeI A'CCGG_T
Cuts at: 5848 5848
Size: 7460
AhdI GACnn_n'nnGTC
Cuts at: 3008 3008
Size: 7460
AleI CACnn'nnGTG
Cuts at: 847 3377 847
Size: 2530 4930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3014 3014
Size: 7460
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2875 2907 2875
Size: 32 7428
AlwNI CAG_nnn'CTG
Cuts at: 245 895 1585 4765 6332 245
Size: 650 690 3180 1567 1373
Fragments arranged by size:
3180 1567 1373 690 650
ApaI G_GGCC'C
Cuts at: 1616 1853 3362 3489 4019 1616
Size: 237 1509 127 530 5057
Fragments arranged by size:
5057 1509 530 237 127
ApaLI G'TGCA_C
Cuts at: 3532 6230 3532
Size: 2698 4762
ApoI r'AATT_y
Cuts at: 1880 2316 3658 4408 4464 6813 7069 1880
Size: 436 1342 750 56 2349 256 2271
Fragments arranged by size:
2349 2271 1342 750 436 256 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 7460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7460
AvaI C'yCGr_G
Cuts at: 194 1525 1731 1973 2708 2906 3697 4480 194
Size: 1331 206 242 735 198 791 783 3174
Fragments arranged by size:
3174 1331 791 783 735 242 206 198
BamHI G'GATC_C
Cuts at: 4485 4485
Size: 7460
BanI G'GyrC_C
Cuts at: 190 2067 2302 4476 4890 5138 5173 190
Size: 1877 235 2174 414 248 35 2477
Fragments arranged by size:
2477 2174 1877 414 248 235 35
BanII G_rGCy'C
Cuts at: 201 578 1326 1352 1616 1853 1983 2508 3185
Size: 377 748 26 264 237 130 525 677
Cuts at: 3185 3251 3362 3482 3489 3719 4019 4474 5504
Size: 66 111 120 7 230 300 455 1030
Cuts at: 5504 201
Size: 2157
Fragments arranged by size:
2157 1030 748 677 525 455 377 300
264 237 230 130 120 111 66 26
7
BbeI G_GCGC'C
Cuts at: 194 4894 5142 194
Size: 4700 248 2512
Fragments arranged by size:
4700 2512 248
BbsI GAAGACnn'nnnn_
Cuts at: 1577 4003 1577
Size: 2426 5034
BbvCI CC'TCA_GC
Cuts at: 151 151
Size: 7460
BciVI GTATCCnnnnn_n'
Cuts at: 2968 5358 6119 7460 2968
Size: 2390 761 1341 2968
Fragments arranged by size:
2968 2390 1341 761
BclI T'GATC_A
Cuts at: 4980 4980
Size: 7460
BglI GCCn_nnn'nGGC
Cuts at: 3314 3563 3314
Size: 249 7211
BglII A'GATC_T
Cuts at: 1628 4975 7012 1628
Size: 3347 2037 2076
Fragments arranged by size:
3347 2076 2037
BlpI GC'TnA_GC
Cuts at: 2557 4572 2557
Size: 2015 5445
Bme1580I G_kGCm'C
Cuts at: 335 1616 1853 2072 3362 3489 3536 4019 5085
Size: 1281 237 219 1290 127 47 483 1066
Cuts at: 5085 5178 6234 335
Size: 93 1056 1561
Fragments arranged by size:
1561 1290 1281 1066 1056 483 237 219
127 93 47
BmgBI CAC'GTC
Cuts at: 3756 4341 3756
Size: 585 6875
BmrI ACTGGGnnnn_n'
Cuts at: 3963 4672 4753 5088 7232 3963
Size: 709 81 335 2144 4191
Fragments arranged by size:
4191 2144 709 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1225 4017 4053 5827 1225
Size: 2792 36 1774 2858
Fragments arranged by size:
2858 2792 1774 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 790 2076 3624 3927 4114 4225 438
Size: 352 1286 1548 303 187 111 3673
Fragments arranged by size:
3673 1548 1286 352 303 187 111
Bpu10I CC'TnA_GC
Cuts at: 151 151
Size: 7460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2990 4636 5515 6007 6305 2990
Size: 1646 879 492 298 4145
Fragments arranged by size:
4145 1646 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 788 1126 1651 2260 2485 2927 4384 7164 788
Size: 338 525 609 225 442 1457 2780 1084
Fragments arranged by size:
2780 1457 1084 609 525 442 338 225
BsaAI yAC'GTr
Cuts at: 495 1124 4452 4813 495
Size: 629 3328 361 3142
Fragments arranged by size:
3328 3142 629 361
BsaBI GATnn'nnATC
Cuts at: 6 1633 3740 4109 4974 7011 6
Size: 1627 2107 369 865 2037 455
Fragments arranged by size:
2107 2037 1627 865 455 369
BsaHI Gr'CG_yC
Cuts at: 191 2603 4891 5139 5841 7337 191
Size: 2412 2288 248 702 1496 314
Fragments arranged by size:
2412 2288 1496 702 314 248
BsaWI w'CCGG_w
Cuts at: 1281 1760 4649 5170 5848 6122 6269 6680 1281
Size: 479 2889 521 678 274 147 411 2061
Fragments arranged by size:
2889 2061 678 521 479 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 436 466 1970 2000 3916 3946 436
Size: 30 1504 30 1916 30 3950
Fragments arranged by size:
3950 1916 1504 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1198 1446 1453 1561 1636 2029 2195 2377 3529
Size: 248 7 108 75 393 166 182 1152
Cuts at: 3529 3924 1198
Size: 395 4734
Fragments arranged by size:
4734 1152 395 393 248 182 166 108
75 7
BseYI C'CCAG_C
Cuts at: 560 899 2153 2642 3355 3382 3388 3872 4885
Size: 339 1254 489 713 27 6 484 1013
Cuts at: 4885 5643 6220 6716 560
Size: 758 577 496 1304
Fragments arranged by size:
1304 1254 1013 758 713 577 496 489
484 339 27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1295 1417 1579 1295
Size: 122 162 7176
Fragments arranged by size:
7176 162 122
BsiEI CG_ry'CG
Cuts at: 104 5002 5048 6256 104
Size: 4898 46 1208 1308
Fragments arranged by size:
4898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 236 578 1208 1983 3251 3482 3536 4474 5252
Size: 342 630 775 1268 231 54 938 778
Cuts at: 5252 5442 6234 236
Size: 190 792 1462
Fragments arranged by size:
1462 1268 938 792 778 775 630 342
231 190 54
BsmI GAATG_Cn'
Cuts at: 753 2091 753
Size: 1338 6122
BsmBI CGTCTCn'nnnn_
Cuts at: 633 4041 633
Size: 3408 4052
Bsp1286I G_dGCh'C
Cuts at: 201 236 335 578 1208 1326 1352 1616 1853
Size: 35 99 243 630 118 26 264 237
Cuts at: 1853 1983 2072 2508 3185 3251 3362 3482 3489
Size: 130 89 436 677 66 111 120 7
Cuts at: 3489 3536 3719 4019 4474 5085 5178 5252 5442
Size: 47 183 300 455 611 93 74 190
Cuts at: 5442 5504 6234 201
Size: 62 730 1427
Fragments arranged by size:
1427 730 677 630 611 455 436 300
264 243 237 190 183 130 120 118
111 99 93 89 74 66 62 47
35 26 7
BspEI T'CCGG_A
Cuts at: 1760 6680 1760
Size: 4920 2540
BspHI T'CATG_A
Cuts at: 347 1490 6636 347
Size: 1143 5146 1171
Fragments arranged by size:
5146 1171 1143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2150 2334 4510 4724 5026 5407 2150
Size: 184 2176 214 302 381 4203
Fragments arranged by size:
4203 2176 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 935 1966 5752 5806 7290 7455 50
Size: 885 1031 3786 54 1484 165 55
Fragments arranged by size:
3786 1484 1031 885 165 55 54
BsrDI GCAATG_nn'
Cuts at: 764 817 998 2577 2996 3407 5372 764
Size: 53 181 1579 419 411 1965 2852
Fragments arranged by size:
2852 1965 1579 419 411 181 53
BsrFI r'CCGG_y
Cuts at: 5458 5639 5848 5458
Size: 181 209 7070
Fragments arranged by size:
7070 209 181
BssSI C'ACGA_G
Cuts at: 5731 6089 5731
Size: 358 7102
BstAPI GCAn_nnn'nTGC
Cuts at: 895 4837 895
Size: 3942 3518
BstBI TT'CG_AA
Cuts at: 110 5821 110
Size: 5711 1749
BstEII G'GTnAC_C
Cuts at: 840 1365 3867 840
Size: 525 2502 4433
Fragments arranged by size:
4433 2502 525
BstXI CCAn_nnnn'nTGG
Cuts at: 2736 3894 4026 4138 4386 2736
Size: 1158 132 112 248 5810
Fragments arranged by size:
5810 1158 248 132 112
BstYI r'GATC_y
Cuts at: 1 340 1196 1278 1628 3239 4485 4952 4975
Size: 339 856 82 350 1611 1246 467 23
Cuts at: 4975 5310 5556 6568 6654 6666 7012 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1611 1246 1012 856 467 449 350 346
339 335 246 86 82 23 12
BstZ17I GTA'TAC
Cuts at: 4416 4416
Size: 7460
Bsu36I CC'TnA_GG
Cuts at: 163 163
Size: 7460
BtgI C'CryG_G
Cuts at: 125 659 2628 3282 3893 125
Size: 534 1969 654 611 3692
Fragments arranged by size:
3692 1969 654 611 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 639 5410 5591 5754 639
Size: 4771 181 163 2345
Fragments arranged by size:
4771 2345 181 163
BtsI GCAGTG_nn'
Cuts at: 181 335 426 893 1348 3372 3384 3569 3962
Size: 154 91 467 455 2024 12 185 393
Cuts at: 3962 4254 4308 4360 4703 181
Size: 292 54 52 343 2938
Fragments arranged by size:
2938 2024 467 455 393 343 292 185
154 91 54 52 12
ClaI AT'CG_AT
Cuts at: 2874 2874
Size: 7460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 981 1016 1851 1886 3074 3109 981
Size: 35 835 35 1188 35 5332
Fragments arranged by size:
5332 1188 835 35 35 35
DraI TTT'AAA
Cuts at: 4459 4459
Size: 7460
DraIII CAC_nnn'GTG
Cuts at: 3688 4353 3688
Size: 665 6795
DrdI GACnn_nn'nnGTC
Cuts at: 3142 3160 5166 6024 3142
Size: 18 2006 858 4578
Fragments arranged by size:
4578 2006 858 18
EaeI y'GGCC_r
Cuts at: 378 595 2474 3127 3434 4096 5045 5219 5610
Size: 217 1879 653 307 662 949 174 391
Cuts at: 5610 5637 6982 378
Size: 27 1345 856
Fragments arranged by size:
1879 1345 949 856 662 653 391 307
217 174 27
EagI C'GGCC_G
Cuts at: 5045 5045
Size: 7460
EarI CTCTTCn'nnn_
Cuts at: 2592 2764 3262 5483 5693 2592
Size: 172 498 2221 210 4359
Fragments arranged by size:
4359 2221 498 210 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3054 3241 3313 3408 5668 5978 6124 7270 3054
Size: 187 72 95 2260 310 146 1146 3244
Fragments arranged by size:
3244 2260 1146 310 187 146 95 72
EcoICRI GAG'CTC
Cuts at: 576 1981 3249 3480 4472 576
Size: 1405 1268 231 992 3564
Fragments arranged by size:
3564 1405 1268 992 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 438 790 1111 2008 2076 3624 3927 4114
Size: 8 352 321 897 68 1548 303 187
Cuts at: 4114 4225 5285 5717 6464 6732 430
Size: 111 1060 432 747 268 1158
Fragments arranged by size:
1548 1158 1060 897 747 432 352 321
303 268 187 111 68 8
EcoO109I rG'GnC_Cy
Cuts at: 479 675 1246 1612 1613 1850 2521 4599 479
Size: 196 571 366 1 237 671 2078 3340
Fragments arranged by size:
3340 2078 671 571 366 237 196 1
EcoRI G'AATT_C
Cuts at: 2316 4464 2316
Size: 2148 5312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2462 5479 2462
Size: 3017 4443
FspI TGC'GCA
Cuts at: 5241 5241
Size: 7460
HaeII r_GCGC'y
Cuts at: 194 209 867 2564 4179 4894 5142 6164 7143
Size: 15 658 1697 1615 715 248 1022 979
Cuts at: 7143 194
Size: 511
Fragments arranged by size:
1697 1615 1022 979 715 658 511 248
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 931 963 1970 2002 2649 2681 2926 2958 2994
Size: 32 1007 32 647 32 245 32 36
Cuts at: 2994 3026 3790 3822 5302 5332 5334 5364 931
Size: 32 764 32 1480 30 2 30 3027
Fragments arranged by size:
3027 1480 1007 764 647 245 36 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 855 1146 2607 3498 4499 855
Size: 291 1461 891 1001 3816
Fragments arranged by size:
3816 1461 1001 891 291
HindIII A'AGCT_T
Cuts at: 796 796
Size: 7460
KasI G'GCGC_C
Cuts at: 190 4890 5138 190
Size: 4700 248 2512
Fragments arranged by size:
4700 2512 248
KpnI G_GTAC'C
Cuts at: 4480 4480
Size: 7460
MluI A'CGCG_T
Cuts at: 6893 6893
Size: 7460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 2745 4221 4403 6131 6315 983
Size: 1762 1476 182 1728 184 2128
Fragments arranged by size:
2128 1762 1728 1476 184 182
MscI TGG'CCA
Cuts at: 380 597 2476 3129 5221 380
Size: 217 1879 653 2092 2619
Fragments arranged by size:
2619 2092 1879 653 217
MslI CAynn'nnrTG
Cuts at: 352 658 759 847 1038 1948 1951 2734 2764
Size: 306 101 88 191 910 3 783 30
Cuts at: 2764 3076 3377 3580 4024 4339 5449 5576 352
Size: 312 301 203 444 315 1110 127 2236
Fragments arranged by size:
2236 1110 910 783 444 315 312 306
301 203 191 127 101 88 30 3
MspA1I CmG'CkG
Cuts at: 174 903 923 1332 1373 2001 2044 2630 3272
Size: 729 20 409 41 628 43 586 642
Cuts at: 3272 3773 4571 4885 5245 6258 6503 174
Size: 501 798 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 798 729 642 628 586 501
409 360 314 245 43 41 20
NaeI GCC'GGC
Cuts at: 5641 5641
Size: 7460
NarI GG'CG_CC
Cuts at: 191 4891 5139 191
Size: 4700 248 2512
Fragments arranged by size:
4700 2512 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 7460
NgoMIV G'CCGG_C
Cuts at: 5639 5639
Size: 7460
NspI r_CATG'y
Cuts at: 4513 5013 5920 4513
Size: 500 907 6053
Fragments arranged by size:
6053 907 500
PasI CC'CwG_GG
Cuts at: 475 1798 475
Size: 1323 6137
PciI A'CATG_T
Cuts at: 5916 5916
Size: 7460
PflMI CCAn_nnn'nTGG
Cuts at: 726 4105 726
Size: 3379 4081
PfoI T'CCnGG_A
Cuts at: 1578 3242 3545 1578
Size: 1664 303 5493
Fragments arranged by size:
5493 1664 303
PmeI GTTT'AAAC
Cuts at: 4459 4459
Size: 7460
PmlI CAC'GTG
Cuts at: 1124 1124
Size: 7460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6599 6631 6599
Size: 32 7428
PpuMI rG'GwC_Cy
Cuts at: 675 675
Size: 7460
PspOMI G'GGCC_C
Cuts at: 1612 1849 3358 3485 4015 1612
Size: 237 1509 127 530 5057
Fragments arranged by size:
5057 1509 530 237 127
PstI C_TGCA'G
Cuts at: 173 1343 2753 3379 3709 4303 4507 5192 173
Size: 1170 1410 626 330 594 204 685 2441
Fragments arranged by size:
2441 1410 1170 685 626 594 330 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7460
PvuII CAG'CTG
Cuts at: 174 1332 2001 2044 3272 4885 5245 174
Size: 1158 669 43 1228 1613 360 2389
Fragments arranged by size:
2389 1613 1228 1158 669 360 43
RsrII CG'GwC_CG
Cuts at: 5655 5655
Size: 7460
SacI G_AGCT'C
Cuts at: 578 1983 3251 3482 4474 578
Size: 1405 1268 231 992 3564
Fragments arranged by size:
3564 1405 1268 992 231
SacII CC_GC'GG
Cuts at: 2631 2631
Size: 7460
SalI G'TCGA_C
Cuts at: 1144 4497 1144
Size: 3353 4107
SapI GCTCTTCn'nnn_
Cuts at: 5483 5693 5483
Size: 210 7250
SbfI CC_TGCA'GG
Cuts at: 4507 4507
Size: 7460
ScaI AGT'ACT
Cuts at: 6977 6977
Size: 7460
SexAI A'CCwGG_T
Cuts at: 1218 3089 3500 1218
Size: 1871 411 5178
Fragments arranged by size:
5178 1871 411
SfcI C'TryA_G
Cuts at: 33 169 237 1339 2226 2749 3375 3705 4299
Size: 136 68 1102 887 523 626 330 594
Cuts at: 4299 4503 5188 6181 6372 33
Size: 204 685 993 191 1121
Fragments arranged by size:
1121 1102 993 887 685 626 594 523
330 204 191 136 68
SfoI GGC'GCC
Cuts at: 192 4892 5140 192
Size: 4700 248 2512
Fragments arranged by size:
4700 2512 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7460
SmaI CCC'GGG
Cuts at: 196 1975 2710 2908 4482 196
Size: 1779 735 198 1574 3174
Fragments arranged by size:
3174 1779 1574 735 198
SmlI C'TyrA_G
Cuts at: 1731 3005 4615 5530 6022 6284 1731
Size: 1274 1610 915 492 262 2907
Fragments arranged by size:
2907 1610 1274 915 492 262
SnaBI TAC'GTA
Cuts at: 4452 4452
Size: 7460
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7460
SphI G_CATG'C
Cuts at: 4513 5013 4513
Size: 500 6960
SrfI GCCC'GGGC
Cuts at: 196 196
Size: 7460
SspI AAT'ATT
Cuts at: 4073 4435 4073
Size: 362 7098
StuI AGG'CCT
Cuts at: 1478 2809 1478
Size: 1331 6129
StyI C'CwwG_G
Cuts at: 125 516 1605 2846 4594 4948 125
Size: 391 1089 1241 1748 354 2637
Fragments arranged by size:
2637 1748 1241 1089 391 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 439 552 2158 2167 2326 3886 5043 5176 5635
Size: 113 1606 9 159 1560 1157 133 459
Cuts at: 5635 5844 439
Size: 209 2055
Fragments arranged by size:
2055 1606 1560 1157 459 209 159 133
113 9
TatI w'GTAC_w
Cuts at: 4133 6975 4133
Size: 2842 4618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1257 1289 4232 4264 6977 7009 1257
Size: 32 2943 32 2713 32 1708
Fragments arranged by size:
2943 2713 1708 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 667 5257 667
Size: 4590 2870
XbaI T'CTAG_A
Cuts at: 1593 4491 1593
Size: 2898 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 814 882 3075 3309 3379 3429 4261 4955 814
Size: 68 2193 234 70 50 832 694 3319
Fragments arranged by size:
3319 2193 832 694 234 70 68 50
XhoI C'TCGA_G
Cuts at: 1731 1731
Size: 7460
XmaI C'CCGG_G
Cuts at: 194 1973 2708 2906 4480 194
Size: 1779 735 198 1574 3174
Fragments arranged by size:
3174 1779 1574 735 198
XmnI GAAnn'nnTTC
Cuts at: 1574 1750 1574
Size: 176 7284
ZraI GAC'GTC
Cuts at: 2604 2604
Size: 7460
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BcgI BfrBI BmtI BsiWI
BsrGI BssHII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SwaI