Restriction map for
pF1KSDA0287
(Circular) (Six-base) MAPSORT of: pF1KSDA0287.seq Check: 6985 from: 1 to: 7529
pF1KSDA0287 7529 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 4457 4457
Size: 7529
AccI GT'mk_AC
Cuts at: 2057 2167 4567 2057
Size: 110 2400 5019
Fragments arranged by size:
5019 2400 110
Acc65I G'GTAC_C
Cuts at: 4545 4545
Size: 7529
AclI AA'CG_TT
Cuts at: 1263 7369 1263
Size: 6106 1423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 653 1396 2249 2844 4040 4169 4190 5354
Size: 96 743 853 595 1196 129 21 1164
Cuts at: 5354 5786 6533 6801 557
Size: 432 747 268 1285
Fragments arranged by size:
1285 1196 1164 853 747 743 595 432
268 129 96 21
AflIII A'CryG_T
Cuts at: 4881 5985 6962 4881
Size: 1104 977 5448
Fragments arranged by size:
5448 1104 977
AgeI A'CCGG_T
Cuts at: 761 5917 761
Size: 5156 2373
AhdI GACnn_n'nnGTC
Cuts at: 288 2990 4482 288
Size: 2702 1492 3335
Fragments arranged by size:
3335 2702 1492
AlwNI CAG_nnn'CTG
Cuts at: 3850 4834 6401 3850
Size: 984 1567 4978
Fragments arranged by size:
4978 1567 984
ApaI G_GGCC'C
Cuts at: 2141 2141
Size: 7529
ApaLI G'TGCA_C
Cuts at: 6299 6299
Size: 7529
ApoI r'AATT_y
Cuts at: 703 1150 1435 1868 2515 4533 6882 7138 703
Size: 447 285 433 647 2018 2349 256 1094
Fragments arranged by size:
2349 2018 1094 647 447 433 285 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7529
AvaI C'yCGr_G
Cuts at: 2645 4549 2645
Size: 1904 5625
BamHI G'GATC_C
Cuts at: 4554 4554
Size: 7529
BanI G'GyrC_C
Cuts at: 4417 4545 4959 5207 5242 4417
Size: 128 414 248 35 6704
Fragments arranged by size:
6704 414 248 128 35
BanII G_rGCy'C
Cuts at: 961 1853 2141 3677 3725 4049 4543 5573 961
Size: 892 288 1536 48 324 494 1030 2917
Fragments arranged by size:
2917 1536 1030 892 494 324 288 48
BbeI G_GCGC'C
Cuts at: 4963 5211 4963
Size: 248 7281
BbsI GAAGACnn'nnnn_
Cuts at: 146 545 2435 2891 3014 3044 4136 4223 146
Size: 399 1890 456 123 30 1092 87 3452
Fragments arranged by size:
3452 1890 1092 456 399 123 87 30
BbvCI CC'TCA_GC
Cuts at: 455 1054 3530 455
Size: 599 2476 4454
Fragments arranged by size:
4454 2476 599
BciVI GTATCCnnnnn_n'
Cuts at: 5427 6188 7529 5427
Size: 761 1341 5427
Fragments arranged by size:
5427 1341 761
BclI T'GATC_A
Cuts at: 4343 5049 4343
Size: 706 6823
BfrBI ATG'CAT
Cuts at: 862 1173 862
Size: 311 7218
BglI GCCn_nnn'nGGC
Cuts at: 1312 1312
Size: 7529
BglII A'GATC_T
Cuts at: 5044 7081 5044
Size: 2037 5492
BlpI GC'TnA_GC
Cuts at: 2097 3552 3938 3980 4001 4641 2097
Size: 1455 386 42 21 640 4985
Fragments arranged by size:
4985 1455 640 386 42 21
Bme1580I G_kGCm'C
Cuts at: 2141 5154 5247 6303 2141
Size: 3013 93 1056 3367
Fragments arranged by size:
3367 3013 1056 93
BmgBI CAC'GTC
Cuts at: 159 159
Size: 7529
BmrI ACTGGGnnnn_n'
Cuts at: 252 2135 4741 4822 5157 7301 252
Size: 1883 2606 81 335 2144 480
Fragments arranged by size:
2606 2144 1883 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1204 2671 2757 5896 1204
Size: 1467 86 3139 2837
Fragments arranged by size:
3139 2837 1467 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 2234 2501 3248 3532 378
Size: 1856 267 747 284 4375
Fragments arranged by size:
4375 1856 747 284 267
Bpu10I CC'TnA_GC
Cuts at: 455 1045 1054 3530 4380 455
Size: 590 9 2476 850 3604
Fragments arranged by size:
3604 2476 850 590 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 4705 5584 6076 6374 2927
Size: 1778 879 492 298 4082
Fragments arranged by size:
4082 1778 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 275 1282 2590 2962 2977 7233 275
Size: 1007 1308 372 15 4256 571
Fragments arranged by size:
4256 1308 1007 571 372 15
BsaAI yAC'GTr
Cuts at: 3654 4521 4882 3654
Size: 867 361 6301
Fragments arranged by size:
6301 867 361
BsaBI GATnn'nnATC
Cuts at: 6 5043 7080 6
Size: 5037 2037 455
Fragments arranged by size:
5037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1776 4454 4960 5208 5910 7406 1776
Size: 2678 506 248 702 1496 1899
Fragments arranged by size:
2678 1899 1496 702 506 248
BsaWI w'CCGG_w
Cuts at: 248 761 4718 5239 5917 6191 6338 6749 248
Size: 513 3957 521 678 274 147 411 1028
Fragments arranged by size:
3957 1028 678 521 513 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 3372 3402 3676 3706 376
Size: 30 2966 30 274 30 4199
Fragments arranged by size:
4199 2966 274 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2950 2980 2995 2950
Size: 30 15 7484
Fragments arranged by size:
7484 30 15
BseYI C'CCAG_C
Cuts at: 1582 1587 2527 3415 3633 4954 5712 6289 6785
Size: 5 940 888 218 1321 758 577 496
Cuts at: 6785 1582
Size: 2326
Fragments arranged by size:
2326 1321 940 888 758 577 496 218
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1467 3626 4139 547
Size: 920 2159 513 3937
Fragments arranged by size:
3937 2159 920 513
BsiEI CG_ry'CG
Cuts at: 104 762 5071 5117 6325 104
Size: 658 4309 46 1208 1308
Fragments arranged by size:
4309 1308 1208 658 46
BsiHKAI G_wGCw'C
Cuts at: 1163 1496 1853 2714 4381 4543 5321 5511 6303
Size: 333 357 861 1667 162 778 190 792
Cuts at: 6303 1163
Size: 2389
Fragments arranged by size:
2389 1667 861 792 778 357 333 190
162
BsmI GAATG_Cn'
Cuts at: 864 3757 4285 4309 864
Size: 2893 528 24 4084
Fragments arranged by size:
4084 2893 528 24
Bsp1286I G_dGCh'C
Cuts at: 961 1163 1496 1853 2141 2714 3677 3725 4049
Size: 202 333 357 288 573 963 48 324
Cuts at: 4049 4381 4543 5154 5247 5321 5511 5573 6303
Size: 332 162 611 93 74 190 62 730
Cuts at: 6303 961
Size: 2187
Fragments arranged by size:
2187 963 730 611 573 357 333 332
324 288 202 190 162 93 74 62
48
BspEI T'CCGG_A
Cuts at: 6749 6749
Size: 7529
BspHI T'CATG_A
Cuts at: 2724 6705 2724
Size: 3981 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4111 4579 4793 5095 5476 4111
Size: 468 214 302 381 6164
Fragments arranged by size:
6164 468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5821 5875 7359 7524 50
Size: 5771 54 1484 165 55
Fragments arranged by size:
5771 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1106 2598 5441 1106
Size: 1492 2843 3194
Fragments arranged by size:
3194 2843 1492
BsrFI r'CCGG_y
Cuts at: 653 761 5527 5708 5917 653
Size: 108 4766 181 209 2265
Fragments arranged by size:
4766 2265 209 181 108
BssSI C'ACGA_G
Cuts at: 278 2558 5800 6158 278
Size: 2280 3242 358 1649
Fragments arranged by size:
3242 2280 1649 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3149 3643 3850 4906 3149
Size: 494 207 1056 5772
Fragments arranged by size:
5772 1056 494 207
BstBI TT'CG_AA
Cuts at: 110 5890 110
Size: 5780 1749
BstEII G'GTnAC_C
Cuts at: 232 232
Size: 7529
BstYI r'GATC_y
Cuts at: 1 330 580 2488 3105 4554 5021 5044 5379
Size: 329 250 1908 617 1449 467 23 335
Cuts at: 5379 5625 6637 6723 6735 7081 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1908 1449 1012 617 467 449 346 335
329 250 246 86 23 12
BtgI C'CryG_G
Cuts at: 325 684 2988 3376 325
Size: 359 2304 388 4478
Fragments arranged by size:
4478 2304 388 359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5479 5660 5823 5479
Size: 181 163 7185
Fragments arranged by size:
7185 181 163
BtsI GCAGTG_nn'
Cuts at: 4772 4772
Size: 7529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3744 3779 3744
Size: 35 7494
DraI TTT'AAA
Cuts at: 4528 4528
Size: 7529
DraIII CAC_nnn'GTG
Cuts at: 690 1221 690
Size: 531 6998
DrdI GACnn_nn'nnGTC
Cuts at: 5235 6093 5235
Size: 858 6671
EaeI y'GGCC_r
Cuts at: 546 1821 3422 5114 5288 5679 5706 7051 546
Size: 1275 1601 1692 174 391 27 1345 1024
Fragments arranged by size:
1692 1601 1345 1275 1024 391 174 27
EagI C'GGCC_G
Cuts at: 5114 5114
Size: 7529
EarI CTCTTCn'nnn_
Cuts at: 186 735 1945 3581 4145 4166 4474 5552 5762
Size: 549 1210 1636 564 21 308 1078 210
Cuts at: 5762 186
Size: 1953
Fragments arranged by size:
1953 1636 1210 1078 564 549 308 210
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5737 6047 6193 7339 5737
Size: 310 146 1146 5927
Fragments arranged by size:
5927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1851 4541 1851
Size: 2690 4839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 557 653 1396 2234 2249 2501 2844 3248
Size: 179 96 743 838 15 252 343 404
Cuts at: 3248 3532 4040 4169 4190 5354 5786 6533 6801
Size: 284 508 129 21 1164 432 747 268
Cuts at: 6801 378
Size: 1106
Fragments arranged by size:
1164 1106 838 747 743 508 432 404
343 284 268 252 179 129 96 21
15
EcoNI CCTnn'n_nnAGG
Cuts at: 454 2399 454
Size: 1945 5584
EcoO109I rG'GnC_Cy
Cuts at: 2137 3689 4668 2137
Size: 1552 979 4998
Fragments arranged by size:
4998 1552 979
EcoRI G'AATT_C
Cuts at: 2515 4533 2515
Size: 2018 5511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1014 1482 1905 5548 1014
Size: 468 423 3643 2995
Fragments arranged by size:
3643 2995 468 423
FspI TGC'GCA
Cuts at: 5310 5310
Size: 7529
HaeII r_GCGC'y
Cuts at: 933 4963 5211 6233 7212 933
Size: 4030 248 1022 979 1250
Fragments arranged by size:
4030 1250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 376 397 408 412 429 444 535
Size: 32 70 21 11 4 17 15 91
Cuts at: 535 567 918 950 954 986 2053 2085 2976
Size: 32 351 32 4 32 1067 32 891
Cuts at: 2976 3008 3673 3705 4468 4500 5371 5401 5403
Size: 32 665 32 763 32 871 30 2
Cuts at: 5403 5433 274
Size: 30 2370
Fragments arranged by size:
2370 1067 891 871 763 665 351 91
70 32 32 32 32 32 32 32
32 30 30 21 17 15 11 4
4 2
HincII GTy'rAC
Cuts at: 3159 3630 4568 3159
Size: 471 938 6120
Fragments arranged by size:
6120 938 471
HindIII A'AGCT_T
Cuts at: 360 360
Size: 7529
KasI G'GCGC_C
Cuts at: 4959 5207 4959
Size: 248 7281
KpnI G_GTAC'C
Cuts at: 4549 4549
Size: 7529
MfeI C'AATT_G
Cuts at: 1085 3002 1085
Size: 1917 5612
MluI A'CGCG_T
Cuts at: 6962 6962
Size: 7529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1233 6200 6384 1233
Size: 4967 184 2378
Fragments arranged by size:
4967 2378 184
MscI TGG'CCA
Cuts at: 548 5290 548
Size: 4742 2787
MslI CAynn'nnrTG
Cuts at: 1033 1110 1544 3668 5518 5645 1033
Size: 77 434 2124 1850 127 2917
Fragments arranged by size:
2917 2124 1850 434 127 77
MspA1I CmG'CkG
Cuts at: 287 327 843 3237 3850 4640 4954 5314 6327
Size: 40 516 2394 613 790 314 360 1013
Cuts at: 6327 6572 287
Size: 245 1244
Fragments arranged by size:
2394 1244 1013 790 613 516 360 314
245 40
NaeI GCC'GGC
Cuts at: 5710 5710
Size: 7529
NarI GG'CG_CC
Cuts at: 4960 5208 4960
Size: 248 7281
NcoI C'CATG_G
Cuts at: 2988 2988
Size: 7529
NdeI CA'TA_TG
Cuts at: 1107 2073 3482 1107
Size: 966 1409 5154
Fragments arranged by size:
5154 1409 966
NgoMIV G'CCGG_C
Cuts at: 5708 5708
Size: 7529
NsiI A_TGCA'T
Cuts at: 864 1175 864
Size: 311 7218
NspI r_CATG'y
Cuts at: 4582 5082 5989 4582
Size: 500 907 6122
Fragments arranged by size:
6122 907 500
PciI A'CATG_T
Cuts at: 5985 5985
Size: 7529
PflMI CCAn_nnn'nTGG
Cuts at: 441 1221 3256 441
Size: 780 2035 4714
Fragments arranged by size:
4714 2035 780
PfoI T'CCnGG_A
Cuts at: 482 2478 482
Size: 1996 5533
PmeI GTTT'AAAC
Cuts at: 4528 4528
Size: 7529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6668 6700 6668
Size: 32 7497
PpuMI rG'GwC_Cy
Cuts at: 3689 3689
Size: 7529
PspOMI G'GGCC_C
Cuts at: 2137 2137
Size: 7529
PspXI vC'TCGA_Gb
Cuts at: 2645 2645
Size: 7529
PstI C_TGCA'G
Cuts at: 3396 3855 4044 4576 5261 3396
Size: 459 189 532 685 5664
Fragments arranged by size:
5664 685 532 459 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7529
PvuII CAG'CTG
Cuts at: 3237 3850 4954 5314 3237
Size: 613 1104 360 5452
Fragments arranged by size:
5452 1104 613 360
RsrII CG'GwC_CG
Cuts at: 5724 5724
Size: 7529
SacI G_AGCT'C
Cuts at: 1853 4543 1853
Size: 2690 4839
SacII CC_GC'GG
Cuts at: 328 328
Size: 7529
SalI G'TCGA_C
Cuts at: 4566 4566
Size: 7529
SapI GCTCTTCn'nnn_
Cuts at: 735 4145 4166 4474 5552 5762 735
Size: 3410 21 308 1078 210 2502
Fragments arranged by size:
3410 2502 1078 308 210 21
SbfI CC_TGCA'GG
Cuts at: 4576 4576
Size: 7529
ScaI AGT'ACT
Cuts at: 4441 7046 4441
Size: 2605 4924
SfcI C'TryA_G
Cuts at: 33 501 3392 3851 4040 4572 5257 6250 6441
Size: 468 2891 459 189 532 685 993 191
Cuts at: 6441 33
Size: 1121
Fragments arranged by size:
2891 1121 993 685 532 468 459 191
189
SfoI GGC'GCC
Cuts at: 4961 5209 4961
Size: 248 7281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7529
SgrAI Cr'CCGG_yG
Cuts at: 653 653
Size: 7529
SmaI CCC'GGG
Cuts at: 4551 4551
Size: 7529
SmlI C'TyrA_G
Cuts at: 2645 2942 4684 5599 6091 6353 2645
Size: 297 1742 915 492 262 3821
Fragments arranged by size:
3821 1742 915 492 297 262
SnaBI TAC'GTA
Cuts at: 4521 4521
Size: 7529
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7529
SphI G_CATG'C
Cuts at: 4582 5082 4582
Size: 500 7029
StyI C'CwwG_G
Cuts at: 1310 2988 4663 5017 1310
Size: 1678 1675 354 3822
Fragments arranged by size:
3822 1678 1675 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3208 3598 5112 5245 5704 5913 3208
Size: 390 1514 133 459 209 4824
Fragments arranged by size:
4824 1514 459 390 209 133
TatI w'GTAC_w
Cuts at: 3170 4439 7044 3170
Size: 1269 2605 3655
Fragments arranged by size:
3655 2605 1269
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 758 790 3181 3213 3372 3404 7046 7078 758
Size: 32 2391 32 159 32 3642 32 1209
Fragments arranged by size:
3642 2391 1209 159 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2434 3013 5326 2434
Size: 579 2313 4637
Fragments arranged by size:
4637 2313 579
XbaI T'CTAG_A
Cuts at: 4560 4560
Size: 7529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 226 284 5024 226
Size: 58 4740 2731
Fragments arranged by size:
4740 2731 58
XhoI C'TCGA_G
Cuts at: 2645 2645
Size: 7529
XmaI C'CCGG_G
Cuts at: 4549 4549
Size: 7529
XmnI GAAnn'nnTTC
Cuts at: 4441 4441
Size: 7529
ZraI GAC'GTC
Cuts at: 4455 4455
Size: 7529
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BmtI BsiWI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI EcoRV FseI FspAI HpaI NheI
NotI NruI PacI PasI PmlI PshAI PsiI PsrI
SanDI SexAI SfiI SrfI SspI StuI SwaI