Restriction map for
pF1KSDA0289
(Circular) (Six-base) MAPSORT of: pF1KSDA0289.seq Check: 1251 from: 1 to: 7021
pF1KSDA0289 7021 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 649 4057 649
Size: 3408 3613
Acc65I G'GTAC_C
Cuts at: 2531 2738 4035 2531
Size: 207 1297 5517
Fragments arranged by size:
5517 1297 207
AclI AA'CG_TT
Cuts at: 6861 6861
Size: 7021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 1181 2499 3817 4844 5276 6025 6293 557
Size: 624 1318 1318 1027 432 749 268 1285
Fragments arranged by size:
1318 1318 1285 1027 749 624 432 268
AflIII A'CryG_T
Cuts at: 3485 3949 4371 5477 6454 3485
Size: 464 422 1106 977 4052
Fragments arranged by size:
4052 1106 977 464 422
AgeI A'CCGG_T
Cuts at: 5409 5409
Size: 7021
AleI CACnn'nnGTG
Cuts at: 1547 3238 1547
Size: 1691 5330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1403 1771 1403
Size: 368 6653
AlwNI CAG_nnn'CTG
Cuts at: 462 1442 2358 2409 3109 3966 4324 5893 462
Size: 980 916 51 700 857 358 1569 1590
Fragments arranged by size:
1590 1569 980 916 857 700 358 51
ApaLI G'TGCA_C
Cuts at: 5791 5791
Size: 7021
ApoI r'AATT_y
Cuts at: 1965 3736 4023 6374 6630 1965
Size: 1771 287 2351 256 2356
Fragments arranged by size:
2356 2351 1771 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7021
AvaI C'yCGr_G
Cuts at: 348 4039 348
Size: 3691 3330
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3856 3889 3856
Size: 33 6988
BamHI G'GATC_C
Cuts at: 207 575 1215 1596 4044 207
Size: 368 640 381 2448 3184
Fragments arranged by size:
3184 2448 640 381 368
BanI G'GyrC_C
Cuts at: 505 811 2130 2531 2738 2887 3420 3881 4035
Size: 306 1319 401 207 149 533 461 154
Cuts at: 4035 4449 4697 4732 505
Size: 414 248 35 2794
Fragments arranged by size:
2794 1319 533 461 414 401 306 248
207 154 149 35
BanII G_rGCy'C
Cuts at: 143 317 2477 3091 3227 3929 4033 5063 143
Size: 174 2160 614 136 702 104 1030 2101
Fragments arranged by size:
2160 2101 1030 702 614 174 136 104
BbeI G_GCGC'C
Cuts at: 2134 4453 4701 2134
Size: 2319 248 4454
Fragments arranged by size:
4454 2319 248
BbsI GAAGACnn'nnnn_
Cuts at: 2789 3556 3952 2789
Size: 767 396 5858
Fragments arranged by size:
5858 767 396
BbvCI CC'TCA_GC
Cuts at: 571 710 1762 2611 3808 571
Size: 139 1052 849 1197 3784
Fragments arranged by size:
3784 1197 1052 849 139
BciVI GTATCCnnnnn_n'
Cuts at: 3982 4917 5680 7021 3982
Size: 935 763 1341 3982
Fragments arranged by size:
3982 1341 935 763
BclI T'GATC_A
Cuts at: 1289 3452 4539 1289
Size: 2163 1087 3771
Fragments arranged by size:
3771 2163 1087
BglI GCCn_nnn'nGGC
Cuts at: 1795 1795
Size: 7021
BglII A'GATC_T
Cuts at: 410 2465 3602 4004 4534 6573 410
Size: 2055 1137 402 530 2039 858
Fragments arranged by size:
2055 2039 1137 858 530 402
BlpI GC'TnA_GC
Cuts at: 2993 3361 3928 4131 2993
Size: 368 567 203 5883
Fragments arranged by size:
5883 567 368 203
Bme1580I G_kGCm'C
Cuts at: 510 761 1518 2017 2441 4644 4737 5795 510
Size: 251 757 499 424 2203 93 1058 1736
Fragments arranged by size:
2203 1736 1058 757 499 424 251 93
BmgBI CAC'GTC
Cuts at: 204 1335 3540 3950 204
Size: 1131 2205 410 3275
Fragments arranged by size:
3275 2205 1131 410
BmrI ACTGGGnnnn_n'
Cuts at: 1571 2510 3927 4231 4312 4647 6793 1571
Size: 939 1417 304 81 335 2146 1799
Fragments arranged by size:
2146 1799 1417 939 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1722 2484 5386 1722
Size: 762 2902 3357
Fragments arranged by size:
3357 2902 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 397 427 468 484 789 1083 1435 1470
Size: 153 30 41 16 305 294 352 35
Cuts at: 1470 1918 2104 2464 2794 2927 3028 3131 3268
Size: 448 186 360 330 133 101 103 137
Cuts at: 3268 3407 3510 244
Size: 139 103 3755
Fragments arranged by size:
3755 448 360 352 330 305 294 186
153 139 137 133 103 103 101 41
35 30 16
Bpu10I CC'TnA_GC
Cuts at: 283 571 710 817 1376 1762 2611 3102 3808
Size: 288 139 107 559 386 849 491 706
Cuts at: 3808 283
Size: 3496
Fragments arranged by size:
3496 849 706 559 491 386 288 139
107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 3853 4195 5074 5568 5866 3569
Size: 284 342 879 494 298 4724
Fragments arranged by size:
4724 879 494 342 298 284
BsaI GGTCTCn'nnnn_
Cuts at: 2230 2828 3787 6725 2230
Size: 598 959 2938 2526
Fragments arranged by size:
2938 2526 959 598
BsaAI yAC'GTr
Cuts at: 4011 4372 4011
Size: 361 6660
BsaBI GATnn'nnATC
Cuts at: 6 1294 4533 6572 6
Size: 1288 3239 2039 455
Fragments arranged by size:
3239 2039 1288 455
BsaHI Gr'CG_yC
Cuts at: 2131 4450 4698 5402 6898 2131
Size: 2319 248 704 1496 2254
Fragments arranged by size:
2319 2254 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2558 4208 4729 5409 5683 5830 6241 2558
Size: 1650 521 680 274 147 411 3338
Fragments arranged by size:
3338 1650 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 211 241 344 374 1224 1254 1735 1765 211
Size: 30 103 30 850 30 481 30 5467
Fragments arranged by size:
5467 850 481 103 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 580 1317 1879 2476 2734 2836 3289 3970
Size: 267 737 562 597 258 102 453 681
Cuts at: 3970 313
Size: 3364
Fragments arranged by size:
3364 737 681 597 562 453 267 258
102
BseYI C'CCAG_C
Cuts at: 164 1196 3226 3794 4444 5202 5781 6277 164
Size: 1032 2030 568 650 758 579 496 908
Fragments arranged by size:
2030 1032 908 758 650 579 568 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 2672 3301 811
Size: 1861 629 4531
Fragments arranged by size:
4531 1861 629
BsiEI CG_ry'CG
Cuts at: 104 194 4561 4607 5817 104
Size: 90 4367 46 1210 1308
Fragments arranged by size:
4367 1308 1210 90 46
BsiHKAI G_wGCw'C
Cuts at: 1327 1382 3091 3103 3929 4033 4811 5001 5795
Size: 55 1709 12 826 104 778 190 794
Cuts at: 5795 1327
Size: 2553
Fragments arranged by size:
2553 1709 826 794 778 190 104 55
12
BsmI GAATG_Cn'
Cuts at: 1573 1619 1573
Size: 46 6975
Bsp1286I G_dGCh'C
Cuts at: 143 317 510 761 1327 1382 1518 2017 2441
Size: 174 193 251 566 55 136 499 424
Cuts at: 2441 2477 3091 3103 3227 3929 4033 4644 4737
Size: 36 614 12 124 702 104 611 93
Cuts at: 4737 4811 5001 5063 5795 143
Size: 74 190 62 732 1369
Fragments arranged by size:
1369 732 702 614 611 566 499 424
251 193 190 174 136 124 104 93
74 62 55 36 12
BspEI T'CCGG_A
Cuts at: 6241 6241
Size: 7021
BspHI T'CATG_A
Cuts at: 6197 6197
Size: 7021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2742 4069 4283 4585 4966 2742
Size: 1327 214 302 381 4797
Fragments arranged by size:
4797 1327 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2826 5311 5365 6851 7016 50
Size: 2776 2485 54 1486 165 55
Fragments arranged by size:
2776 2485 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 786 966 2622 3413 3828 4931 786
Size: 180 1656 791 415 1103 2876
Fragments arranged by size:
2876 1656 1103 791 415 180
BsrFI r'CCGG_y
Cuts at: 171 196 656 1923 2997 5017 5198 5409 171
Size: 25 460 1267 1074 2020 181 211 1783
Fragments arranged by size:
2020 1783 1267 1074 460 211 181 25
BssSI C'ACGA_G
Cuts at: 1318 2390 2648 3220 5290 5650 1318
Size: 1072 258 572 2070 360 2689
Fragments arranged by size:
2689 2070 1072 572 360 258
BstAPI GCAn_nnn'nTGC
Cuts at: 2921 3818 4396 2921
Size: 897 578 5546
Fragments arranged by size:
5546 897 578
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7021
BstEII G'GTnAC_C
Cuts at: 3012 3012
Size: 7021
BstXI CCAn_nnnn'nTGG
Cuts at: 222 2340 2898 222
Size: 2118 558 4345
Fragments arranged by size:
4345 2118 558
BstYI r'GATC_y
Cuts at: 1 207 410 575 1215 1596 2465 3602 3669
Size: 206 203 165 640 381 869 1137 67
Cuts at: 3669 4004 4044 4511 4534 4869 5115 6129 6215
Size: 335 40 467 23 335 246 1014 86
Cuts at: 6215 6227 6573 1
Size: 12 346 449
Fragments arranged by size:
1137 1014 869 640 467 449 381 346
335 335 246 206 203 165 86 67
40 23 12
BstZ17I GTA'TAC
Cuts at: 650 650
Size: 7021
Bsu36I CC'TnA_GG
Cuts at: 2840 3940 2840
Size: 1100 5921
BtgI C'CryG_G
Cuts at: 125 188 1502 1651 3212 3563 125
Size: 63 1314 149 1561 351 3583
Fragments arranged by size:
3583 1561 1314 351 149 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3762 4969 5150 5313 3762
Size: 1207 181 163 5470
Fragments arranged by size:
5470 1207 181 163
BtsI GCAGTG_nn'
Cuts at: 256 463 3146 4262 256
Size: 207 2683 1116 3015
Fragments arranged by size:
3015 2683 1116 207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 789 824 3135 3170 789
Size: 35 2311 35 4640
Fragments arranged by size:
4640 2311 35 35
DraI TTT'AAA
Cuts at: 4018 4018
Size: 7021
DraIII CAC_nnn'GTG
Cuts at: 3497 3497
Size: 7021
DrdI GACnn_nn'nnGTC
Cuts at: 4725 5585 4725
Size: 860 6161
EaeI y'GGCC_r
Cuts at: 185 191 1796 2429 2594 4604 4778 5169 5196
Size: 6 1605 633 165 2010 174 391 27
Cuts at: 5196 6543 185
Size: 1347 663
Fragments arranged by size:
2010 1605 1347 663 633 391 174 165
27 6
EagI C'GGCC_G
Cuts at: 191 4604 191
Size: 4413 2608
EarI CTCTTCn'nnn_
Cuts at: 331 557 691 1474 3619 5042 5252 331
Size: 226 134 783 2145 1423 210 2100
Fragments arranged by size:
2145 2100 1423 783 226 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1134 3937 5227 5539 5685 6831 1134
Size: 2803 1290 312 146 1146 1324
Fragments arranged by size:
2803 1324 1290 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3089 3927 4031 3089
Size: 838 104 6079
Fragments arranged by size:
6079 838 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 397 427 468 484 557 789 1083 1181
Size: 153 30 41 16 73 232 294 98
Cuts at: 1181 1435 1470 1918 2104 2464 2499 2794 2927
Size: 254 35 448 186 360 35 295 133
Cuts at: 2927 3028 3131 3268 3407 3510 3817 4844 5276
Size: 101 103 137 139 103 307 1027 432
Cuts at: 5276 6025 6293 244
Size: 749 268 972
Fragments arranged by size:
1027 972 749 448 432 360 307 295
294 268 254 232 186 153 139 137
133 103 103 101 98 73 41 35
35 30 16
EcoO109I rG'GnC_Cy
Cuts at: 681 2607 3696 3766 3804 3936 4158 681
Size: 1926 1089 70 38 132 222 3544
Fragments arranged by size:
3544 1926 1089 222 132 70 38
EcoRI G'AATT_C
Cuts at: 3736 4023 3736
Size: 287 6734
EcoRV GAT'ATC
Cuts at: 432 432
Size: 7021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 1099 1296 3867 5038 342
Size: 757 197 2571 1171 2325
Fragments arranged by size:
2571 2325 1171 757 197
FspI TGC'GCA
Cuts at: 337 778 4800 337
Size: 441 4022 2558
Fragments arranged by size:
4022 2558 441
HaeII r_GCGC'y
Cuts at: 663 2134 2668 3525 4453 4701 5725 6704 663
Size: 1471 534 857 928 248 1024 979 980
Fragments arranged by size:
1471 1024 980 979 928 857 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 2200 2232 3104 3136 3367 3399 3594
Size: 32 1824 32 872 32 231 32 195
Cuts at: 3594 3626 4861 4891 4893 4923 344
Size: 32 1235 30 2 30 2442
Fragments arranged by size:
2442 1824 1235 872 231 195 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1338 4058 1338
Size: 2720 4301
HindIII A'AGCT_T
Cuts at: 2197 3849 2197
Size: 1652 5369
KasI G'GCGC_C
Cuts at: 2130 4449 4697 2130
Size: 2319 248 4454
Fragments arranged by size:
4454 2319 248
KpnI G_GTAC'C
Cuts at: 2535 2742 4039 2535
Size: 207 1297 5517
Fragments arranged by size:
5517 1297 207
MluI A'CGCG_T
Cuts at: 6454 6454
Size: 7021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1088 2108 2651 5692 5876 1088
Size: 1020 543 3041 184 2233
Fragments arranged by size:
3041 2233 1020 543 184
MscI TGG'CCA
Cuts at: 187 1798 2431 2596 4780 187
Size: 1611 633 165 2184 2428
Fragments arranged by size:
2428 2184 1611 633 165
MslI CAynn'nnrTG
Cuts at: 247 500 1547 1834 2071 2167 3238 3484 5008
Size: 253 1047 287 237 96 1071 246 1524
Cuts at: 5008 5135 247
Size: 127 2133
Fragments arranged by size:
2133 1524 1071 1047 287 253 246 237
127 96
MspA1I CmG'CkG
Cuts at: 1200 1304 1428 1490 1973 2112 2760 4130 4444
Size: 104 124 62 483 139 648 1370 314
Cuts at: 4444 4804 5819 6064 1200
Size: 360 1015 245 2157
Fragments arranged by size:
2157 1370 1015 648 483 360 314 245
139 124 104 62
NaeI GCC'GGC
Cuts at: 173 198 658 5200 173
Size: 25 460 4542 1994
Fragments arranged by size:
4542 1994 460 25
NarI GG'CG_CC
Cuts at: 2131 4450 4698 2131
Size: 2319 248 4454
Fragments arranged by size:
4454 2319 248
NcoI C'CATG_G
Cuts at: 125 1502 1651 125
Size: 1377 149 5495
Fragments arranged by size:
5495 1377 149
NgoMIV G'CCGG_C
Cuts at: 171 196 656 5198 171
Size: 25 460 4542 1994
Fragments arranged by size:
4542 1994 460 25
NruI TCG'CGA
Cuts at: 5382 5382
Size: 7021
NspI r_CATG'y
Cuts at: 1372 1752 3489 4072 4572 5481 1372
Size: 380 1737 583 500 909 2912
Fragments arranged by size:
2912 1737 909 583 500 380
PasI CC'CwG_GG
Cuts at: 1496 1496
Size: 7021
PciI A'CATG_T
Cuts at: 3485 5477 3485
Size: 1992 5029
PflMI CCAn_nnn'nTGG
Cuts at: 1442 2127 2631 1442
Size: 685 504 5832
Fragments arranged by size:
5832 685 504
PfoI T'CCnGG_A
Cuts at: 519 824 839 1526 2138 2696 3245 3665 519
Size: 305 15 687 612 558 549 420 3875
Fragments arranged by size:
3875 687 612 558 549 420 305 15
PmeI GTTT'AAAC
Cuts at: 4018 4018
Size: 7021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6160 6192 6160
Size: 32 6989
PpuMI rG'GwC_Cy
Cuts at: 681 3696 3766 3804 3936 681
Size: 3015 70 38 132 3766
Fragments arranged by size:
3766 3015 132 70 38
PshAI GACnn'nnGTC
Cuts at: 3131 3131
Size: 7021
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1233 1265 1233
Size: 32 6989
PstI C_TGCA'G
Cuts at: 887 953 1939 1960 2546 4066 4751 887
Size: 66 986 21 586 1520 685 3157
Fragments arranged by size:
3157 1520 986 685 586 66 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7021
PvuII CAG'CTG
Cuts at: 1200 1304 1428 2112 4444 4804 1200
Size: 104 124 684 2332 360 3417
Fragments arranged by size:
3417 2332 684 360 124 104
RsrII CG'GwC_CG
Cuts at: 5214 5214
Size: 7021
SacI G_AGCT'C
Cuts at: 3091 3929 4033 3091
Size: 838 104 6079
Fragments arranged by size:
6079 838 104
SalI G'TCGA_C
Cuts at: 4056 4056
Size: 7021
SanDI GG'GwC_CC
Cuts at: 681 681
Size: 7021
SapI GCTCTTCn'nnn_
Cuts at: 5042 5252 5042
Size: 210 6811
SbfI CC_TGCA'GG
Cuts at: 953 4066 953
Size: 3113 3908
ScaI AGT'ACT
Cuts at: 6538 6538
Size: 7021
SexAI A'CCwGG_T
Cuts at: 1685 1685
Size: 7021
SfcI C'TryA_G
Cuts at: 33 883 949 1935 1956 2542 3888 4062 4747
Size: 850 66 986 21 586 1346 174 685
Cuts at: 4747 5742 5933 33
Size: 995 191 1121
Fragments arranged by size:
1346 1121 995 986 850 685 586 191
174 66 21
SfoI GGC'GCC
Cuts at: 2132 4451 4699 2132
Size: 2319 248 4454
Fragments arranged by size:
4454 2319 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7021
SgrAI Cr'CCGG_yG
Cuts at: 196 656 196
Size: 460 6561
SmaI CCC'GGG
Cuts at: 350 4041 350
Size: 3691 3330
SmlI C'TyrA_G
Cuts at: 3584 3868 4174 5089 5583 5845 3584
Size: 284 306 915 494 262 4760
Fragments arranged by size:
4760 915 494 306 284 262
SnaBI TAC'GTA
Cuts at: 4011 4011
Size: 7021
SpeI A'CTAG_T
Cuts at: 67 3039 3628 67
Size: 2972 589 3460
Fragments arranged by size:
3460 2972 589
SphI G_CATG'C
Cuts at: 4072 4572 4072
Size: 500 6521
SspI AAT'ATT
Cuts at: 1038 1667 1718 1038
Size: 629 51 6341
Fragments arranged by size:
6341 629 51
StyI C'CwwG_G
Cuts at: 125 215 1502 1651 2333 4153 4507 125
Size: 90 1287 149 682 1820 354 2639
Fragments arranged by size:
2639 1820 1287 682 354 149 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 586 2168 4602 4735 5194 5405 586
Size: 1582 2434 133 459 211 2202
Fragments arranged by size:
2434 2202 1582 459 211 133
TatI w'GTAC_w
Cuts at: 1602 6536 1602
Size: 4934 2087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 248 280 1735 1767 3936 3968 6538
Size: 32 5 32 1455 32 2169 32 2570
Cuts at: 6538 6570 211
Size: 32 662
Fragments arranged by size:
2570 2169 1455 662 32 32 32 32
32 5
Tth111I GACn'n_nGTC
Cuts at: 2027 4816 2027
Size: 2789 4232
XbaI T'CTAG_A
Cuts at: 4050 4050
Size: 7021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 630 805 1053 2296 2439 2632 4514 630
Size: 175 248 1243 143 193 1882 3137
Fragments arranged by size:
3137 1882 1243 248 193 175 143
XmaI C'CCGG_G
Cuts at: 348 4039 348
Size: 3691 3330
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII
ClaI EcoNI FseI FspAI HpaI MfeI NdeI NheI
NotI NsiI PacI PmlI PsiI PspOMI PspXI SacII
SfiI SrfI StuI SwaI XhoI XmnI ZraI