Restriction map for
pF1KSDA0290
(Circular) (Six-base) MAPSORT of: pF1KSDA0290.seq Check: 2419 from: 1 to: 5806
pF1KSDA0290 5806 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 807 1044 1404 1776 807
Size: 237 360 372 4837
Fragments arranged by size:
4837 372 360 237
AccI GT'mk_AC
Cuts at: 2842 2842
Size: 5806
Acc65I G'GTAC_C
Cuts at: 2820 2820
Size: 5806
AclI AA'CG_TT
Cuts at: 969 5646 969
Size: 4677 1129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 3629 4061 4810 5078 1883
Size: 1746 432 749 268 2611
Fragments arranged by size:
2611 1746 749 432 268
AflIII A'CryG_T
Cuts at: 1072 1846 3156 4262 5239 1072
Size: 774 1310 1106 977 1639
Fragments arranged by size:
1639 1310 1106 977 774
AgeI A'CCGG_T
Cuts at: 4194 4194
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 1563 1954 1563
Size: 391 5415
AleI CACnn'nnGTG
Cuts at: 802 2464 802
Size: 1662 4144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2408 2558 2408
Size: 150 5656
AlwNI CAG_nnn'CTG
Cuts at: 525 1385 2496 3109 4678 525
Size: 860 1111 613 1569 1653
Fragments arranged by size:
1653 1569 1111 860 613
ApaI G_GGCC'C
Cuts at: 206 2651 206
Size: 2445 3361
ApaLI G'TGCA_C
Cuts at: 1201 4576 1201
Size: 3375 2431
ApoI r'AATT_y
Cuts at: 829 2808 5159 5415 829
Size: 1979 2351 256 1220
Fragments arranged by size:
2351 1979 1220 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5806
AvaI C'yCGr_G
Cuts at: 507 680 1223 1243 1961 2072 2361 2824 507
Size: 173 543 20 718 111 289 463 3489
Fragments arranged by size:
3489 718 543 463 289 173 111 20
BamHI G'GATC_C
Cuts at: 2829 2829
Size: 5806
BanI G'GyrC_C
Cuts at: 1309 1575 1615 1644 1674 2455 2820 3234 3482
Size: 266 40 29 30 781 365 414 248
Cuts at: 3482 3517 1309
Size: 35 3598
Fragments arranged by size:
3598 781 414 365 266 248 40 35
30 29
BanII G_rGCy'C
Cuts at: 206 775 1142 1187 1230 1630 1882 1897 1975
Size: 569 367 45 43 400 252 15 78
Cuts at: 1975 1990 2651 2818 3848 206
Size: 15 661 167 1030 2164
Fragments arranged by size:
2164 1030 661 569 400 367 252 167
78 45 43 15 15
BbeI G_GCGC'C
Cuts at: 1648 3238 3486 1648
Size: 1590 248 3968
Fragments arranged by size:
3968 1590 248
BbsI GAAGACnn'nnnn_
Cuts at: 336 336
Size: 5806
BbvCI CC'TCA_GC
Cuts at: 2152 2152
Size: 5806
BciVI GTATCCnnnnn_n'
Cuts at: 1678 3702 4465 5806 1678
Size: 2024 763 1341 1678
Fragments arranged by size:
2024 1678 1341 763
BclI T'GATC_A
Cuts at: 3324 3324
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 1307 1307
Size: 5806
BglII A'GATC_T
Cuts at: 3319 5358 3319
Size: 2039 3767
BlpI GC'TnA_GC
Cuts at: 2916 2916
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 206 898 1205 1312 1677 2397 2471 2651 3429
Size: 692 307 107 365 720 74 180 778
Cuts at: 3429 3522 4580 206
Size: 93 1058 1432
Fragments arranged by size:
1432 1058 778 720 692 365 307 180
107 93 74
BmrI ACTGGGnnnn_n'
Cuts at: 1900 2090 2364 3016 3097 3432 5578 1900
Size: 190 274 652 81 335 2146 2128
Fragments arranged by size:
2146 2128 652 335 274 190 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 282 2434 2658 4171 282
Size: 2152 224 1513 1917
Fragments arranged by size:
2152 1917 1513 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1214 1465 1485 1700 1753 2578 1075
Size: 139 251 20 215 53 825 4303
Fragments arranged by size:
4303 825 251 215 139 53 20
Bpu10I CC'TnA_GC
Cuts at: 1264 1876 2152 1264
Size: 612 276 4918
Fragments arranged by size:
4918 612 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 2980 3859 4353 4651 430
Size: 2550 879 494 298 1585
Fragments arranged by size:
2550 1585 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 253 1350 1745 2236 2433 5510 253
Size: 1097 395 491 197 3077 549
Fragments arranged by size:
3077 1097 549 491 395 197
BsaAI yAC'GTr
Cuts at: 804 1849 2796 3157 804
Size: 1045 947 361 3453
Fragments arranged by size:
3453 1045 947 361
BsaBI GATnn'nnATC
Cuts at: 6 3318 5357 6
Size: 3312 2039 455
Fragments arranged by size:
3312 2039 455
BsaHI Gr'CG_yC
Cuts at: 512 1645 3235 3483 4187 5683 512
Size: 1133 1590 248 704 1496 635
Fragments arranged by size:
1590 1496 1133 704 635 248
BsaWI w'CCGG_w
Cuts at: 2993 3514 4194 4468 4615 5026 2993
Size: 521 680 274 147 411 3773
Fragments arranged by size:
3773 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 573 603 1402 1432 1942 1972 2338 2368 573
Size: 30 799 30 510 30 366 30 4011
Fragments arranged by size:
4011 799 510 366 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 345 751 1423 1509 1512 1763 2575 2772
Size: 74 406 672 86 3 251 812 197
Cuts at: 2772 271
Size: 3305
Fragments arranged by size:
3305 812 672 406 251 197 86 74
3
BseYI C'CCAG_C
Cuts at: 768 1342 1633 1927 2059 2630 2650 2659 3229
Size: 574 291 294 132 571 20 9 570
Cuts at: 3229 3987 4566 5062 768
Size: 758 579 496 1512
Fragments arranged by size:
1512 758 579 574 571 570 496 294
291 132 20 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 826 1409 1748 2657 517
Size: 309 583 339 909 3666
Fragments arranged by size:
3666 909 583 339 309
BsiEI CG_ry'CG
Cuts at: 104 1136 3346 3392 4602 104
Size: 1032 2210 46 1210 1308
Fragments arranged by size:
2210 1308 1210 1032 46
BsiHKAI G_wGCw'C
Cuts at: 484 1205 2189 2818 3596 3786 4580 484
Size: 721 984 629 778 190 794 1710
Fragments arranged by size:
1710 984 794 778 721 629 190
BsmBI CGTCTCn'nnnn_
Cuts at: 357 1718 357
Size: 1361 4445
Bsp1286I G_dGCh'C
Cuts at: 206 484 775 898 1142 1187 1205 1230 1312
Size: 278 291 123 244 45 18 25 82
Cuts at: 1312 1630 1677 1882 1897 1975 1990 2189 2397
Size: 318 47 205 15 78 15 199 208
Cuts at: 2397 2471 2651 2818 3429 3522 3596 3786 3848
Size: 74 180 167 611 93 74 190 62
Cuts at: 3848 4580 206
Size: 732 1432
Fragments arranged by size:
1432 732 611 318 291 278 244 208
205 199 190 180 167 123 93 82
78 74 74 62 47 45 25 18
15 15
BspEI T'CCGG_A
Cuts at: 5026 5026
Size: 5806
BspHI T'CATG_A
Cuts at: 4982 4982
Size: 5806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 807 1044 1404 1776 2854 3068 3370 3751
Size: 318 237 360 372 1078 214 302 381
Cuts at: 3751 489
Size: 2544
Fragments arranged by size:
2544 1078 381 372 360 318 302 237
214
BsrBI CCG'CTC
Cuts at: 50 333 573 1020 2640 4096 4150 5636 5801
Size: 283 240 447 1620 1456 54 1486 165
Cuts at: 5801 50
Size: 55
Fragments arranged by size:
1620 1486 1456 447 283 240 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1860 3716 1860
Size: 1856 3950
BsrFI r'CCGG_y
Cuts at: 1010 2447 2538 3802 3983 4194 1010
Size: 1437 91 1264 181 211 2622
Fragments arranged by size:
2622 1437 1264 211 181 91
BsrGI T'GTAC_A
Cuts at: 2166 2166
Size: 5806
BssSI C'ACGA_G
Cuts at: 4075 4435 4075
Size: 360 5446
BstAPI GCAn_nnn'nTGC
Cuts at: 702 940 3181 702
Size: 238 2241 3327
Fragments arranged by size:
3327 2241 238
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5806
BstEII G'GTnAC_C
Cuts at: 2734 2734
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 222 1598 1724 2067 2667 222
Size: 1376 126 343 600 3361
Fragments arranged by size:
3361 1376 600 343 126
BstYI r'GATC_y
Cuts at: 1 405 2492 2829 3296 3319 3654 3900 4914
Size: 404 2087 337 467 23 335 246 1014
Cuts at: 4914 5000 5012 5358 1
Size: 86 12 346 449
Fragments arranged by size:
2087 1014 467 449 404 346 337 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 748 748
Size: 5806
BtgI C'CryG_G
Cuts at: 314 731 1274 2046 2459 2666 314
Size: 417 543 772 413 207 3454
Fragments arranged by size:
3454 772 543 417 413 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 289 976 3754 3935 4098 289
Size: 687 2778 181 163 1997
Fragments arranged by size:
2778 1997 687 181 163
BtsI GCAGTG_nn'
Cuts at: 763 1910 2312 2410 3047 763
Size: 1147 402 98 637 3522
Fragments arranged by size:
3522 1147 637 402 98
DraI TTT'AAA
Cuts at: 2803 2803
Size: 5806
DraIII CAC_nnn'GTG
Cuts at: 192 789 192
Size: 597 5209
DrdI GACnn_nn'nnGTC
Cuts at: 2700 3510 4370 2700
Size: 810 860 4136
Fragments arranged by size:
4136 860 810
EaeI y'GGCC_r
Cuts at: 296 1133 1742 2049 2613 3389 3563 3954 3981
Size: 837 609 307 564 776 174 391 27
Cuts at: 3981 5328 296
Size: 1347 774
Fragments arranged by size:
1347 837 776 774 609 564 391 307
174 27
EagI C'GGCC_G
Cuts at: 1133 3389 1133
Size: 2256 3550
EarI CTCTTCn'nnn_
Cuts at: 1175 1902 2749 3827 4037 1175
Size: 727 847 1078 210 2944
Fragments arranged by size:
2944 1078 847 727 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 631 4012 4324 4470 5616 241
Size: 390 3381 312 146 1146 431
Fragments arranged by size:
3381 1146 431 390 312 146
EcoICRI GAG'CTC
Cuts at: 2816 2816
Size: 5806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 1214 1465 1485 1700 1753 1883 2578 3629
Size: 139 251 20 215 53 130 695 1051
Cuts at: 3629 4061 4810 5078 1075
Size: 432 749 268 1803
Fragments arranged by size:
1803 1051 749 695 432 268 251 215
139 130 53 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1064 1301 2422 1064
Size: 237 1121 4448
Fragments arranged by size:
4448 1121 237
EcoO109I rG'GnC_Cy
Cuts at: 177 202 308 320 1602 1720 1791 1860 2132
Size: 25 106 12 1282 118 71 69 272
Cuts at: 2132 2365 2647 2943 177
Size: 233 282 296 3040
Fragments arranged by size:
3040 1282 296 282 272 233 118 106
71 69 25 12
EcoRI G'AATT_C
Cuts at: 2808 2808
Size: 5806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1446 3823 1446
Size: 2377 3429
FspI TGC'GCA
Cuts at: 3585 3585
Size: 5806
HaeII r_GCGC'y
Cuts at: 373 657 1648 2295 3238 3486 4510 5489 373
Size: 284 991 647 943 248 1024 979 690
Fragments arranged by size:
1024 991 979 943 690 647 284 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1402 1434 1549 1581 1940 1972 3646 3676 3678
Size: 32 115 32 359 32 1674 30 2
Cuts at: 3678 3708 1402
Size: 30 3500
Fragments arranged by size:
3500 1674 359 115 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2843 2843
Size: 5806
KasI G'GCGC_C
Cuts at: 1644 3234 3482 1644
Size: 1590 248 3968
Fragments arranged by size:
3968 1590 248
KpnI G_GTAC'C
Cuts at: 2824 2824
Size: 5806
MluI A'CGCG_T
Cuts at: 5239 5239
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1190 4477 4661 1190
Size: 3287 184 2335
Fragments arranged by size:
3287 2335 184
MscI TGG'CCA
Cuts at: 298 2051 3565 298
Size: 1753 1514 2539
Fragments arranged by size:
2539 1753 1514
MslI CAynn'nnrTG
Cuts at: 802 2464 2773 3793 3920 802
Size: 1662 309 1020 127 2688
Fragments arranged by size:
2688 1662 1020 309 127
MspA1I CmG'CkG
Cuts at: 1042 1255 1276 1385 1637 2063 2129 2630 2732
Size: 213 21 109 252 426 66 501 102
Cuts at: 2732 2915 3229 3589 4604 4849 1042
Size: 183 314 360 1015 245 1999
Fragments arranged by size:
1999 1015 501 426 360 314 252 245
213 183 109 102 66 21
NaeI GCC'GGC
Cuts at: 1012 2540 3985 1012
Size: 1528 1445 2833
Fragments arranged by size:
2833 1528 1445
NarI GG'CG_CC
Cuts at: 1645 3235 3483 1645
Size: 1590 248 3968
Fragments arranged by size:
3968 1590 248
NcoI C'CATG_G
Cuts at: 314 731 314
Size: 417 5389
NdeI CA'TA_TG
Cuts at: 777 777
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 1010 2538 3983 1010
Size: 1528 1445 2833
Fragments arranged by size:
2833 1528 1445
NruI TCG'CGA
Cuts at: 4167 4167
Size: 5806
NspI r_CATG'y
Cuts at: 1076 2745 2857 3357 4266 1076
Size: 1669 112 500 909 2616
Fragments arranged by size:
2616 1669 909 500 112
PasI CC'CwG_GG
Cuts at: 1303 1724 1303
Size: 421 5385
PciI A'CATG_T
Cuts at: 1072 4262 1072
Size: 3190 2616
PflMI CCAn_nnn'nTGG
Cuts at: 732 732
Size: 5806
PfoI T'CCnGG_A
Cuts at: 236 999 1052 236
Size: 763 53 4990
Fragments arranged by size:
4990 763 53
PmeI GTTT'AAAC
Cuts at: 2803 2803
Size: 5806
PmlI CAC'GTG
Cuts at: 804 804
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4945 4977 4945
Size: 32 5774
PpuMI rG'GwC_Cy
Cuts at: 177 308 320 1602 1860 2132 2365 177
Size: 131 12 1282 258 272 233 3618
Fragments arranged by size:
3618 1282 272 258 233 131 12
PshAI GACnn'nnGTC
Cuts at: 1362 2524 1362
Size: 1162 4644
PspOMI G'GGCC_C
Cuts at: 202 2647 202
Size: 2445 3361
PspXI vC'TCGA_Gb
Cuts at: 2072 2072
Size: 5806
PstI C_TGCA'G
Cuts at: 956 1041 1240 2291 2387 2851 3536 956
Size: 85 199 1051 96 464 685 3226
Fragments arranged by size:
3226 1051 685 464 199 96 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5806
PvuII CAG'CTG
Cuts at: 1042 1385 1637 2063 2630 3229 3589 1042
Size: 343 252 426 567 599 360 3259
Fragments arranged by size:
3259 599 567 426 360 343 252
RsrII CG'GwC_CG
Cuts at: 3999 3999
Size: 5806
SacI G_AGCT'C
Cuts at: 2818 2818
Size: 5806
SacII CC_GC'GG
Cuts at: 1277 1277
Size: 5806
SalI G'TCGA_C
Cuts at: 2841 2841
Size: 5806
SanDI GG'GwC_CC
Cuts at: 308 1602 2132 2365 308
Size: 1294 530 233 3749
Fragments arranged by size:
3749 1294 530 233
SapI GCTCTTCn'nnn_
Cuts at: 1175 1902 3827 4037 1175
Size: 727 1925 210 2944
Fragments arranged by size:
2944 1925 727 210
SbfI CC_TGCA'GG
Cuts at: 956 2851 956
Size: 1895 3911
ScaI AGT'ACT
Cuts at: 5323 5323
Size: 5806
SexAI A'CCwGG_T
Cuts at: 2780 2780
Size: 5806
SfcI C'TryA_G
Cuts at: 33 952 1037 1043 1236 1638 2287 2383 2847
Size: 919 85 6 193 402 649 96 464
Cuts at: 2847 3532 4527 4718 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 919 685 649 464 402 193
191 96 85 6
SfoI GGC'GCC
Cuts at: 1646 3236 3484 1646
Size: 1590 248 3968
Fragments arranged by size:
3968 1590 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5806
SmaI CCC'GGG
Cuts at: 1225 1963 2363 2826 1225
Size: 738 400 463 4205
Fragments arranged by size:
4205 738 463 400
SmlI C'TyrA_G
Cuts at: 445 2072 2959 3874 4368 4630 445
Size: 1627 887 915 494 262 1621
Fragments arranged by size:
1627 1621 915 887 494 262
SnaBI TAC'GTA
Cuts at: 2796 2796
Size: 5806
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5806
SphI G_CATG'C
Cuts at: 2857 3357 2857
Size: 500 5306
SrfI GCCC'GGGC
Cuts at: 1225 1225
Size: 5806
StuI AGG'CCT
Cuts at: 988 1738 988
Size: 750 5056
StyI C'CwwG_G
Cuts at: 215 314 485 731 983 1739 1916 2938 3292
Size: 99 171 246 252 756 177 1022 354
Cuts at: 3292 215
Size: 2729
Fragments arranged by size:
2729 1022 756 354 252 246 177 171
99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1352 1821 2293 2437 2673 3387 3520 3979 4190
Size: 469 472 144 236 714 133 459 211
Cuts at: 4190 1352
Size: 2968
Fragments arranged by size:
2968 714 472 469 459 236 211 144
133
TatI w'GTAC_w
Cuts at: 2166 5321 2166
Size: 3155 2651
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1942 1974 2132 2164 5323 5355 1942
Size: 32 158 32 3159 32 2393
Fragments arranged by size:
3159 2393 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3601 3601
Size: 5806
XbaI T'CTAG_A
Cuts at: 2835 2835
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 1576 1791 1914 2404 2553 3299 219
Size: 1357 215 123 490 149 746 2726
Fragments arranged by size:
2726 1357 746 490 215 149 123
XhoI C'TCGA_G
Cuts at: 2072 2072
Size: 5806
XmaI C'CCGG_G
Cuts at: 1223 1961 2361 2824 1223
Size: 738 400 463 4205
Fragments arranged by size:
4205 738 463 400
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BcgI
BfrBI BmgBI BmtI BsiWI BsmI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NsiI PacI PsiI PsrI SfiI SgrAI SspI
SwaI XmnI ZraI