Restriction map for
pF1KSDA0293
(Circular) (Six-base) MAPSORT of: pF1KSDA0293.seq Check: 8936 from: 1 to: 7597
pF1KSDA0293 7597 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1276 1276
Size: 7597
AatII G_ACGT'C
Cuts at: 580 580
Size: 7597
AccI GT'mk_AC
Cuts at: 2813 4633 2813
Size: 1820 5777
Acc65I G'GTAC_C
Cuts at: 4611 4611
Size: 7597
AclI AA'CG_TT
Cuts at: 7437 7437
Size: 7597
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1319 2773 3436 3940 5420 5852 6601 6869
Size: 232 1454 663 504 1480 432 749 268
Cuts at: 6869 1087
Size: 1815
Fragments arranged by size:
1815 1480 1454 749 663 504 432 268
232
AfeI AGC'GCT
Cuts at: 764 764
Size: 7597
AflIII A'CryG_T
Cuts at: 2798 3269 4947 6053 7030 2798
Size: 471 1678 1106 977 3365
Fragments arranged by size:
3365 1678 1106 977 471
AgeI A'CCGG_T
Cuts at: 5985 5985
Size: 7597
AhdI GACnn_n'nnGTC
Cuts at: 1488 2928 3381 3558 1488
Size: 1440 453 177 5527
Fragments arranged by size:
5527 1440 453 177
AleI CACnn'nnGTG
Cuts at: 4466 4466
Size: 7597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3056 4484 3056
Size: 1428 6169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4564 4596 4564
Size: 32 7565
AlwNI CAG_nnn'CTG
Cuts at: 1104 1268 1512 1684 1824 1971 2256 3106 3330
Size: 164 244 172 140 147 285 850 224
Cuts at: 3330 3593 3612 4900 6469 1104
Size: 263 19 1288 1569 2232
Fragments arranged by size:
2232 1569 1288 850 285 263 244 224
172 164 147 140 19
ApaI G_GGCC'C
Cuts at: 593 977 1015 1522 1763 2349 2386 3967 4169
Size: 384 38 507 241 586 37 1581 202
Cuts at: 4169 593
Size: 4021
Fragments arranged by size:
4021 1581 586 507 384 241 202 38
37
ApaLI G'TGCA_C
Cuts at: 6367 6367
Size: 7597
ApoI r'AATT_y
Cuts at: 277 1345 4599 6950 7206 277
Size: 1068 3254 2351 256 668
Fragments arranged by size:
3254 2351 1068 668 256
AseI AT'TA_AT
Cuts at: 20 412 20
Size: 392 7205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7597
AvaI C'yCGr_G
Cuts at: 541 628 1008 1501 1762 2037 2626 2779 3259
Size: 87 380 493 261 275 589 153 480
Cuts at: 3259 3638 3655 4162 4615 541
Size: 379 17 507 453 3523
Fragments arranged by size:
3523 589 507 493 480 453 380 379
275 261 153 87 17
BamHI G'GATC_C
Cuts at: 4183 4620 4183
Size: 437 7160
BanI G'GyrC_C
Cuts at: 1663 2274 2292 2394 3040 3651 4402 4611 5025
Size: 611 18 102 646 611 751 209 414
Cuts at: 5025 5273 5308 1663
Size: 248 35 3952
Fragments arranged by size:
3952 751 646 611 611 414 248 209
102 35 18
BanII G_rGCy'C
Cuts at: 548 593 977 1015 1093 1522 1730 1763 1885
Size: 45 384 38 78 429 208 33 122
Cuts at: 1885 1949 2135 2349 2386 2681 2828 2978 3118
Size: 64 186 214 37 295 147 150 140
Cuts at: 3118 3367 3725 3907 3967 4169 4609 5639 548
Size: 249 358 182 60 202 440 1030 2506
Fragments arranged by size:
2506 1030 440 429 384 358 295 249
214 208 202 186 182 150 147 140
122 78 64 60 45 38 37 33
BbeI G_GCGC'C
Cuts at: 1667 2278 2398 4406 5029 5277 1667
Size: 611 120 2008 623 248 3987
Fragments arranged by size:
3987 2008 623 611 248 120
BbsI GAAGACnn'nnnn_
Cuts at: 1307 1394 1487 1307
Size: 87 93 7417
Fragments arranged by size:
7417 93 87
BbvCI CC'TCA_GC
Cuts at: 550 884 1451 2687 3481 4046 4374 550
Size: 334 567 1236 794 565 328 3773
Fragments arranged by size:
3773 1236 794 567 565 334 328
BciVI GTATCCnnnnn_n'
Cuts at: 5493 6256 7597 5493
Size: 763 1341 5493
Fragments arranged by size:
5493 1341 763
BclI T'GATC_A
Cuts at: 848 5115 848
Size: 4267 3330
BglI GCCn_nnn'nGGC
Cuts at: 588 620 1033 1693 3049 588
Size: 32 413 660 1356 5136
Fragments arranged by size:
5136 1356 660 413 32
BglII A'GATC_T
Cuts at: 5110 7149 5110
Size: 2039 5558
BlpI GC'TnA_GC
Cuts at: 1231 2155 2512 3409 4707 1231
Size: 924 357 897 1298 4121
Fragments arranged by size:
4121 1298 924 897 357
Bme1580I G_kGCm'C
Cuts at: 593 977 1015 1429 1522 1763 2297 2349 2386
Size: 384 38 414 93 241 534 52 37
Cuts at: 2386 2624 3586 3967 4169 5220 5313 6371 593
Size: 238 962 381 202 1051 93 1058 1819
Fragments arranged by size:
1819 1058 1051 962 534 414 384 381
241 238 202 93 93 52 38 37
BmgBI CAC'GTC
Cuts at: 1074 1074
Size: 7597
BmrI ACTGGGnnnn_n'
Cuts at: 488 733 971 1194 1432 1723 2499 4460 4807
Size: 245 238 223 238 291 776 1961 347
Cuts at: 4807 4888 5223 7369 488
Size: 81 335 2146 716
Fragments arranged by size:
2146 1961 776 716 347 335 291 245
238 238 223 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2079 3132 3195 3744 5962 2079
Size: 1053 63 549 2218 3714
Fragments arranged by size:
3714 2218 1053 549 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 883 1042 1069 1126 1159 1177 1245 1375
Size: 714 159 27 57 33 18 68 130
Cuts at: 1375 2236 2281 2842 3163 3165 3520 3970 4318
Size: 861 45 561 321 2 355 450 348
Cuts at: 4318 4472 4591 169
Size: 154 119 3175
Fragments arranged by size:
3175 861 714 561 450 355 348 321
159 154 130 119 68 57 45 33
27 18 2
Bpu10I CC'TnA_GC
Cuts at: 550 884 1451 1706 1724 1999 2687 3481 3613
Size: 334 567 255 18 275 688 794 132
Cuts at: 3613 4046 4374 550
Size: 433 328 3773
Fragments arranged by size:
3773 794 688 567 433 334 328 275
255 132 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2665 3730 4771 5650 6144 6442 2665
Size: 1065 1041 879 494 298 3820
Fragments arranged by size:
3820 1065 1041 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 506 7301 506
Size: 6795 802
BsaAI yAC'GTr
Cuts at: 1863 2739 3272 4587 4948 1863
Size: 876 533 1315 361 4512
Fragments arranged by size:
4512 1315 876 533 361
BsaBI GATnn'nnATC
Cuts at: 6 5109 7148 6
Size: 5103 2039 455
Fragments arranged by size:
5103 2039 455
BsaHI Gr'CG_yC
Cuts at: 577 1664 2072 2198 2275 2395 2468 4403 5026
Size: 1087 408 126 77 120 73 1935 623
Cuts at: 5026 5274 5978 7474 577
Size: 248 704 1496 700
Fragments arranged by size:
1935 1496 1087 704 700 623 408 248
126 120 77 73
BsaWI w'CCGG_w
Cuts at: 4784 5305 5985 6259 6406 6817 4784
Size: 521 680 274 147 411 5564
Fragments arranged by size:
5564 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 167 197 2098 2128 2479 2509 3259 3289 4564
Size: 30 1901 30 351 30 750 30 1275
Cuts at: 4564 4594 167
Size: 30 3170
Fragments arranged by size:
3170 1901 1275 750 351 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 538 751 829 1123 1786 1789 2383 2541
Size: 291 213 78 294 663 3 594 158
Cuts at: 2541 2544 2547 2550 3177 3673 3715 3985 4051
Size: 3 3 3 627 496 42 270 66
Cuts at: 4051 4129 4320 4359 4362 4576 247
Size: 78 191 39 3 214 3268
Fragments arranged by size:
3268 663 627 594 496 294 291 270
214 213 191 158 78 78 66 42
39 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 481 893 1363 1495 1515 1522 1570 1605 1650
Size: 412 470 132 20 7 48 35 45
Cuts at: 1650 1866 2895 3091 3586 5020 5778 6357 6853
Size: 216 1029 196 495 1434 758 579 496
Cuts at: 6853 481
Size: 1225
Fragments arranged by size:
1434 1225 1029 758 579 496 495 470
412 216 196 132 48 45 35 20
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 1096 2449 206
Size: 890 1353 5354
Fragments arranged by size:
5354 1353 890
BsiEI CG_ry'CG
Cuts at: 104 2502 2589 2702 2938 4325 5137 5183 6393
Size: 2398 87 113 236 1387 812 46 1210
Cuts at: 6393 104
Size: 1308
Fragments arranged by size:
2398 1387 1308 1210 812 236 113 87
46
BsiHKAI G_wGCw'C
Cuts at: 548 1009 1093 2681 2828 3118 3725 4609 5387
Size: 461 84 1588 147 290 607 884 778
Cuts at: 5387 5577 6371 548
Size: 190 794 1774
Fragments arranged by size:
1774 1588 884 794 778 607 461 290
190 147 84
BsmI GAATG_Cn'
Cuts at: 623 2084 623
Size: 1461 6136
Bsp1286I G_dGCh'C
Cuts at: 548 593 977 1009 1015 1093 1429 1522 1730
Size: 45 384 32 6 78 336 93 208
Cuts at: 1730 1763 1885 1949 2135 2297 2349 2386 2624
Size: 33 122 64 186 162 52 37 238
Cuts at: 2624 2681 2828 2978 3118 3367 3586 3725 3907
Size: 57 147 150 140 249 219 139 182
Cuts at: 3907 3967 4169 4609 5220 5313 5387 5577 5639
Size: 60 202 440 611 93 74 190 62
Cuts at: 5639 6371 548
Size: 732 1774
Fragments arranged by size:
1774 732 611 440 384 336 249 238
219 208 202 190 186 182 162 150
147 140 139 122 93 93 78 74
64 62 60 57 52 45 37 33
32 6
BspEI T'CCGG_A
Cuts at: 6817 6817
Size: 7597
BspHI T'CATG_A
Cuts at: 851 4343 6773 851
Size: 3492 2430 1675
Fragments arranged by size:
3492 2430 1675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 3386 4645 4859 5161 5542 1276
Size: 2110 1259 214 302 381 3331
Fragments arranged by size:
3331 2110 1259 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1143 1753 2288 2313 2400 3179 3197 3575
Size: 1093 610 535 25 87 779 18 378
Cuts at: 3575 4545 5887 5941 7427 7592 50
Size: 970 1342 54 1486 165 55
Fragments arranged by size:
1486 1342 1093 970 779 610 535 378
165 87 55 54 25 18
BsrDI GCAATG_nn'
Cuts at: 5507 5507
Size: 7597
BsrFI r'CCGG_y
Cuts at: 2470 3544 5593 5774 5985 2470
Size: 1074 2049 181 211 4082
Fragments arranged by size:
4082 2049 1074 211 181
BsrGI T'GTAC_A
Cuts at: 2795 2795
Size: 7597
BssHII G'CGCG_C
Cuts at: 2711 2711
Size: 7597
BssSI C'ACGA_G
Cuts at: 5866 6226 5866
Size: 360 7237
BstAPI GCAn_nnn'nTGC
Cuts at: 1512 1863 2256 4972 1512
Size: 351 393 2716 4137
Fragments arranged by size:
4137 2716 393 351
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7597
BstXI CCAn_nnnn'nTGG
Cuts at: 951 1254 1371 3037 951
Size: 303 117 1666 5511
Fragments arranged by size:
5511 1666 303 117
BstYI r'GATC_y
Cuts at: 1 1052 1895 2876 4183 4620 5087 5110 5445
Size: 1051 843 981 1307 437 467 23 335
Cuts at: 5445 5691 6705 6791 6803 7149 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1307 1051 1014 981 843 467 449 437
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 296 1382 3968 296
Size: 1086 2586 3925
Fragments arranged by size:
3925 2586 1086
BtgI C'CryG_G
Cuts at: 125 1730 2502 2601 2944 125
Size: 1605 772 99 343 4778
Fragments arranged by size:
4778 1605 772 343 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2145 5545 5726 5889 2145
Size: 3400 181 163 3853
Fragments arranged by size:
3853 3400 181 163
BtsI GCAGTG_nn'
Cuts at: 1093 1306 3029 3670 4436 4838 1093
Size: 213 1723 641 766 402 3852
Fragments arranged by size:
3852 1723 766 641 402 213
ClaI AT'CG_AT
Cuts at: 145 145
Size: 7597
DraI TTT'AAA
Cuts at: 4594 4594
Size: 7597
DraIII CAC_nnn'GTG
Cuts at: 527 1004 2230 527
Size: 477 1226 5894
Fragments arranged by size:
5894 1226 477
DrdI GACnn_nn'nnGTC
Cuts at: 5301 6161 5301
Size: 860 6737
EaeI y'GGCC_r
Cuts at: 128 716 1154 1289 1373 2314 2324 2574 2586
Size: 588 438 135 84 941 10 250 12
Cuts at: 2586 2699 2852 2935 3015 3248 3566 4322 4511
Size: 113 153 83 80 233 318 756 189
Cuts at: 4511 5180 5354 5745 5772 7119 128
Size: 669 174 391 27 1347 606
Fragments arranged by size:
1347 941 756 669 606 588 438 391
318 250 233 189 174 153 135 113
84 83 80 27 12 10
EagI C'GGCC_G
Cuts at: 2586 2699 2935 4322 5180 2586
Size: 113 236 1387 858 5003
Fragments arranged by size:
5003 1387 858 236 113
EarI CTCTTCn'nnn_
Cuts at: 526 1178 3106 4046 5618 5828 526
Size: 652 1928 940 1572 210 2295
Fragments arranged by size:
2295 1928 1572 940 652 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 259 394 688 816 937 1107 1763 2665 5803
Size: 135 294 128 121 170 656 902 3138
Cuts at: 5803 6115 6261 7407 259
Size: 312 146 1146 449
Fragments arranged by size:
3138 1146 902 656 449 312 294 170
146 135 128 121
EcoICRI GAG'CTC
Cuts at: 546 1091 2679 2826 3116 3723 4607 546
Size: 545 1588 147 290 607 884 3536
Fragments arranged by size:
3536 1588 884 607 545 290 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 883 1042 1069 1087 1126 1159 1177 1245
Size: 714 159 27 18 39 33 18 68
Cuts at: 1245 1319 1375 2236 2281 2773 2842 3163 3165
Size: 74 56 861 45 492 69 321 2
Cuts at: 3165 3436 3520 3940 3970 4318 4472 4591 5420
Size: 271 84 420 30 348 154 119 829
Cuts at: 5420 5852 6601 6869 169
Size: 432 749 268 897
Fragments arranged by size:
897 861 829 749 714 492 432 420
348 321 271 268 159 154 119 84
74 69 68 56 45 39 33 30
27 18 18 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1279 1469 2057 2218 2905 1279
Size: 190 588 161 687 5971
Fragments arranged by size:
5971 687 588 190 161
EcoO109I rG'GnC_Cy
Cuts at: 687 973 1012 1519 1531 1759 2345 2346 2382
Size: 286 39 507 12 228 586 1 36
Cuts at: 2382 2383 2613 3071 3222 3300 3609 3785 3818
Size: 1 230 458 151 78 309 176 33
Cuts at: 3818 3853 3963 3964 4102 4166 4566 4734 687
Size: 35 110 1 138 64 400 168 3550
Fragments arranged by size:
3550 586 507 458 400 309 286 230
228 176 168 151 138 110 78 64
39 36 35 33 12 1 1 1
EcoRI G'AATT_C
Cuts at: 4599 4599
Size: 7597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1011 1344 1965 5614 1011
Size: 333 621 3649 2994
Fragments arranged by size:
3649 2994 621 333
FspI TGC'GCA
Cuts at: 5376 5376
Size: 7597
HaeII r_GCGC'y
Cuts at: 766 1667 1999 2257 2278 2398 4406 5029 5277
Size: 901 332 258 21 120 2008 623 248
Cuts at: 5277 6301 7280 766
Size: 1024 979 1083
Fragments arranged by size:
2008 1083 1024 979 901 623 332 258
248 120 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 562 594 808 840 1099 1131 1474
Size: 32 227 32 214 32 259 32 343
Cuts at: 1474 1506 2015 2047 2098 2130 2302 2334 2914
Size: 32 509 32 51 32 172 32 580
Cuts at: 2914 2946 3259 3291 3367 3399 3544 3576 4186
Size: 32 313 32 76 32 145 32 610
Cuts at: 4186 4218 4500 4532 5437 5467 5469 5499 303
Size: 32 282 32 905 30 2 30 2401
Fragments arranged by size:
2401 905 610 580 509 343 313 282
259 227 214 172 145 76 51 32
32 32 32 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 936 4634 936
Size: 3698 3899
HindIII A'AGCT_T
Cuts at: 333 333
Size: 7597
KasI G'GCGC_C
Cuts at: 1663 2274 2394 4402 5025 5273 1663
Size: 611 120 2008 623 248 3987
Fragments arranged by size:
3987 2008 623 611 248 120
KpnI G_GTAC'C
Cuts at: 4615 4615
Size: 7597
MluI A'CGCG_T
Cuts at: 7030 7030
Size: 7597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1955 2522 6268 6452 1955
Size: 567 3746 184 3100
Fragments arranged by size:
3746 3100 567 184
MscI TGG'CCA
Cuts at: 1291 1375 2326 2576 2854 3250 4513 5356 1291
Size: 84 951 250 278 396 1263 843 3532
Fragments arranged by size:
3532 1263 951 843 396 278 250 84
MslI CAynn'nnrTG
Cuts at: 1957 2011 2986 4466 5584 5711 1957
Size: 54 975 1480 1118 127 3843
Fragments arranged by size:
3843 1480 1118 975 127 54
MspA1I CmG'CkG
Cuts at: 897 924 1732 1782 1821 1845 2154 2378 2504
Size: 27 808 50 39 24 309 224 126
Cuts at: 2504 2603 2772 2994 3327 3447 3690 4494 4706
Size: 99 169 222 333 120 243 804 212
Cuts at: 4706 5020 5380 6395 6640 897
Size: 314 360 1015 245 1854
Fragments arranged by size:
1854 1015 808 804 360 333 314 309
245 243 224 222 212 169 126 120
99 50 39 27 24
NaeI GCC'GGC
Cuts at: 2472 5776 2472
Size: 3304 4293
NarI GG'CG_CC
Cuts at: 1664 2275 2395 4403 5026 5274 1664
Size: 611 120 2008 623 248 3987
Fragments arranged by size:
3987 2008 623 611 248 120
NcoI C'CATG_G
Cuts at: 125 2944 125
Size: 2819 4778
NgoMIV G'CCGG_C
Cuts at: 2470 5774 2470
Size: 3304 4293
NruI TCG'CGA
Cuts at: 5958 5958
Size: 7597
NspI r_CATG'y
Cuts at: 2802 2928 2991 3444 4648 5148 6057 2802
Size: 126 63 453 1204 500 909 4342
Fragments arranged by size:
4342 1204 909 500 453 126 63
PasI CC'CwG_GG
Cuts at: 969 1281 2279 3226 969
Size: 312 998 947 5340
Fragments arranged by size:
5340 998 947 312
PciI A'CATG_T
Cuts at: 2798 6053 2798
Size: 3255 4342
PflMI CCAn_nnn'nTGG
Cuts at: 1287 1916 3024 3398 3471 1287
Size: 629 1108 374 73 5413
Fragments arranged by size:
5413 1108 629 374 73
PfoI T'CCnGG_A
Cuts at: 914 2213 3236 3787 3855 914
Size: 1299 1023 551 68 4656
Fragments arranged by size:
4656 1299 1023 551 68
PmeI GTTT'AAAC
Cuts at: 4594 4594
Size: 7597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4303 4335 4564 4596 6736 6768 4303
Size: 32 229 32 2140 32 5132
Fragments arranged by size:
5132 2140 229 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3071 3300 3609 3785 3818 3853 4102 3071
Size: 229 309 176 33 35 249 6566
Fragments arranged by size:
6566 309 249 229 176 35 33
PspOMI G'GGCC_C
Cuts at: 589 973 1011 1518 1759 2345 2382 3963 4165
Size: 384 38 507 241 586 37 1581 202
Cuts at: 4165 589
Size: 4021
Fragments arranged by size:
4021 1581 586 507 384 241 202 38
37
PstI C_TGCA'G
Cuts at: 476 662 1100 1273 3602 3626 4294 4642 5327
Size: 186 438 173 2329 24 668 348 685
Cuts at: 5327 476
Size: 2746
Fragments arranged by size:
2746 2329 685 668 438 348 186 173
24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7597
PvuII CAG'CTG
Cuts at: 924 1782 1821 2994 3447 3690 5020 5380 924
Size: 858 39 1173 453 243 1330 360 3141
Fragments arranged by size:
3141 1330 1173 858 453 360 243 39
RsrII CG'GwC_CG
Cuts at: 5790 5790
Size: 7597
SacI G_AGCT'C
Cuts at: 548 1093 2681 2828 3118 3725 4609 548
Size: 545 1588 147 290 607 884 3536
Fragments arranged by size:
3536 1588 884 607 545 290 147
SacII CC_GC'GG
Cuts at: 1733 2505 2604 1733
Size: 772 99 6726
Fragments arranged by size:
6726 772 99
SalI G'TCGA_C
Cuts at: 4632 4632
Size: 7597
SanDI GG'GwC_CC
Cuts at: 3818 3818
Size: 7597
SapI GCTCTTCn'nnn_
Cuts at: 3106 5618 5828 3106
Size: 2512 210 4875
Fragments arranged by size:
4875 2512 210
SbfI CC_TGCA'GG
Cuts at: 4642 4642
Size: 7597
ScaI AGT'ACT
Cuts at: 7114 7114
Size: 7597
SfcI C'TryA_G
Cuts at: 33 472 658 1096 1269 3598 3622 3780 4290
Size: 439 186 438 173 2329 24 158 510
Cuts at: 4290 4638 5323 6318 6509 33
Size: 348 685 995 191 1121
Fragments arranged by size:
2329 1121 995 685 510 439 438 348
191 186 173 158 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 1033 1033
Size: 7597
SfoI GGC'GCC
Cuts at: 1665 2276 2396 4404 5027 5275 1665
Size: 611 120 2008 623 248 3987
Fragments arranged by size:
3987 2008 623 611 248 120
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7597
SmaI CCC'GGG
Cuts at: 630 1503 1764 3640 4164 4617 630
Size: 873 261 1876 524 453 3610
Fragments arranged by size:
3610 1876 873 524 453 261
SmlI C'TyrA_G
Cuts at: 2680 3745 4750 5665 6159 6421 2680
Size: 1065 1005 915 494 262 3856
Fragments arranged by size:
3856 1065 1005 915 494 262
SnaBI TAC'GTA
Cuts at: 4587 4587
Size: 7597
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7597
SphI G_CATG'C
Cuts at: 2991 3444 4648 5148 2991
Size: 453 1204 500 5440
Fragments arranged by size:
5440 1204 500 453
SspI AAT'ATT
Cuts at: 157 157
Size: 7597
StuI AGG'CCT
Cuts at: 1327 3682 4340 1327
Size: 2355 658 4584
Fragments arranged by size:
4584 2355 658
StyI C'CwwG_G
Cuts at: 125 827 1040 2354 2944 3067 4098 4472 4729
Size: 702 213 1314 590 123 1031 374 257
Cuts at: 4729 5083 125
Size: 354 2639
Fragments arranged by size:
2639 1314 1031 702 590 374 354 257
213 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 616 1875 3899 4009 5178 5311 5770 5981 616
Size: 1259 2024 110 1169 133 459 211 2232
Fragments arranged by size:
2232 2024 1259 1169 459 211 133 110
TatI w'GTAC_w
Cuts at: 699 1989 2795 3206 7112 699
Size: 1290 806 411 3906 1184
Fragments arranged by size:
3906 1290 1184 806 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2479 2511 7114 7146 2479
Size: 32 4603 32 2930
Fragments arranged by size:
4603 2930 32 32
Tth111I GACn'n_nGTC
Cuts at: 5392 5392
Size: 7597
XbaI T'CTAG_A
Cuts at: 2088 4626 2088
Size: 2538 5059
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 976 1176 2142 4443 5090 976
Size: 200 966 2301 647 3483
Fragments arranged by size:
3483 2301 966 647 200
XmaI C'CCGG_G
Cuts at: 628 1501 1762 3638 4162 4615 628
Size: 873 261 1876 524 453 3610
Fragments arranged by size:
3610 1876 873 524 453 261
XmnI GAAnn'nnTTC
Cuts at: 1966 1966
Size: 7597
ZraI GAC'GTC
Cuts at: 578 578
Size: 7597
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BcgI BfrBI BmtI BsiWI
BsmBI BstEII BstZ17I CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SexAI SgrAI SrfI SwaI XhoI