Restriction map for
pF1KSDA0313
(Circular) (Six-base) MAPSORT of: pF1KSDA0313.seq Check: 8092 from: 1 to: 7636
pF1KSDA0313 7636 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 3877 3877
Size: 7636
AccI GT'mk_AC
Cuts at: 2789 4672 2789
Size: 1883 5753
Acc65I G'GTAC_C
Cuts at: 946 4650 946
Size: 3704 3932
AclI AA'CG_TT
Cuts at: 7476 7476
Size: 7636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1905 2251 2416 2491 2843 3088 3419 3455
Size: 490 346 165 75 352 245 331 36
Cuts at: 3455 4405 5459 5891 6640 6908 1415
Size: 950 1054 432 749 268 2143
Fragments arranged by size:
2143 1054 950 749 490 432 352 346
331 268 245 165 75 36
AflIII A'CryG_T
Cuts at: 4986 6092 7069 4986
Size: 1106 977 5553
Fragments arranged by size:
5553 1106 977
AgeI A'CCGG_T
Cuts at: 6024 6024
Size: 7636
AhdI GACnn_n'nnGTC
Cuts at: 642 934 2975 642
Size: 292 2041 5303
Fragments arranged by size:
5303 2041 292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 7604
AlwNI CAG_nnn'CTG
Cuts at: 3234 3553 4939 6508 3234
Size: 319 1386 1569 4362
Fragments arranged by size:
4362 1569 1386 319
ApaLI G'TGCA_C
Cuts at: 6406 6406
Size: 7636
ApoI r'AATT_y
Cuts at: 508 1192 1478 2255 2310 2447 2532 2834 3383
Size: 684 286 777 55 137 85 302 549
Cuts at: 3383 3566 4349 4638 6989 7245 508
Size: 183 783 289 2351 256 899
Fragments arranged by size:
2351 899 783 777 684 549 302 289
286 256 183 137 85 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 7636
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7636
AvaI C'yCGr_G
Cuts at: 1092 1301 4091 4654 1092
Size: 209 2790 563 4074
Fragments arranged by size:
4074 2790 563 209
AvrII C'CTAG_G
Cuts at: 3675 3675
Size: 7636
BamHI G'GATC_C
Cuts at: 3975 4659 3975
Size: 684 6952
BanI G'GyrC_C
Cuts at: 946 1576 2574 3638 4650 5064 5312 5347 946
Size: 630 998 1064 1012 414 248 35 3235
Fragments arranged by size:
3235 1064 1012 998 630 414 248 35
BanII G_rGCy'C
Cuts at: 4289 4322 4648 5678 4289
Size: 33 326 1030 6247
Fragments arranged by size:
6247 1030 326 33
BbeI G_GCGC'C
Cuts at: 3642 5068 5316 3642
Size: 1426 248 5962
Fragments arranged by size:
5962 1426 248
BbsI GAAGACnn'nnnn_
Cuts at: 389 407 1019 2130 3094 4100 4136 389
Size: 18 612 1111 964 1006 36 3889
Fragments arranged by size:
3889 1111 1006 964 612 36 18
BciVI GTATCCnnnnn_n'
Cuts at: 5532 6295 7636 5532
Size: 763 1341 5532
Fragments arranged by size:
5532 1341 763
BclI T'GATC_A
Cuts at: 1971 1994 3717 4029 5154 1971
Size: 23 1723 312 1125 4453
Fragments arranged by size:
4453 1723 1125 312 23
BfrBI ATG'CAT
Cuts at: 969 3454 969
Size: 2485 5151
BglI GCCn_nnn'nGGC
Cuts at: 3043 4488 3043
Size: 1445 6191
BglII A'GATC_T
Cuts at: 1635 2640 5149 7188 1635
Size: 1005 2509 2039 2083
Fragments arranged by size:
2509 2083 2039 1005
BlpI GC'TnA_GC
Cuts at: 2272 4746 2272
Size: 2474 5162
Bme1580I G_kGCm'C
Cuts at: 1581 3332 4379 5259 5352 6410 1581
Size: 1751 1047 880 93 1058 2807
Fragments arranged by size:
2807 1751 1058 1047 880 93
BmrI ACTGGGnnnn_n'
Cuts at: 2731 3518 4080 4131 4846 4927 5262 7408 2731
Size: 787 562 51 715 81 335 2146 2959
Fragments arranged by size:
2959 2146 787 715 562 335 81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 654 6001 198
Size: 456 5347 1833
Fragments arranged by size:
5347 1833 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3176 3176
Size: 7636
Bpu10I CC'TnA_GC
Cuts at: 1954 2231 2815 1954
Size: 277 584 6775
Fragments arranged by size:
6775 584 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 3867 4810 5689 6183 6481 1784
Size: 2083 943 879 494 298 2939
Fragments arranged by size:
2939 2083 943 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2094 2927 3178 7340 2094
Size: 833 251 4162 2390
Fragments arranged by size:
4162 2390 833 251
BsaAI yAC'GTr
Cuts at: 4626 4987 4626
Size: 361 7275
BsaBI GATnn'nnATC
Cuts at: 6 1753 2482 5148 7187 6
Size: 1747 729 2666 2039 455
Fragments arranged by size:
2666 2039 1747 729 455
BsaHI Gr'CG_yC
Cuts at: 3639 3874 4105 5065 5313 6017 7513 3639
Size: 235 231 960 248 704 1496 3762
Fragments arranged by size:
3762 1496 960 704 248 235 231
BsaWI w'CCGG_w
Cuts at: 476 4823 5344 6024 6298 6445 6856 476
Size: 4347 521 680 274 147 411 1256
Fragments arranged by size:
4347 1256 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 282 312 863 893 3504 3534 4294 4324 282
Size: 30 551 30 2611 30 760 30 3594
Fragments arranged by size:
3594 2611 760 551 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3109 3464 4606 3109
Size: 355 1142 6139
Fragments arranged by size:
6139 1142 355
BseYI C'CCAG_C
Cuts at: 144 806 3245 3929 4100 4459 5059 5817 6396
Size: 662 2439 684 171 359 600 758 579
Cuts at: 6396 6892 144
Size: 496 888
Fragments arranged by size:
2439 888 758 684 662 600 579 496
359 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 3610 2497
Size: 1113 6523
BsiEI CG_ry'CG
Cuts at: 104 5176 5222 6432 104
Size: 5072 46 1210 1308
Fragments arranged by size:
5072 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 4648 5426 5616 6410 4648
Size: 778 190 794 5874
Fragments arranged by size:
5874 794 778 190
BsmI GAATG_Cn'
Cuts at: 984 2530 3648 984
Size: 1546 1118 4972
Fragments arranged by size:
4972 1546 1118
Bsp1286I G_dGCh'C
Cuts at: 1581 3332 4289 4322 4379 4648 5259 5352 5426
Size: 1751 957 33 57 269 611 93 74
Cuts at: 5426 5616 5678 6410 1581
Size: 190 62 732 2807
Fragments arranged by size:
2807 1751 957 732 611 269 190 93
74 62 57 33
BspEI T'CCGG_A
Cuts at: 6856 6856
Size: 7636
BspHI T'CATG_A
Cuts at: 1991 6812 1991
Size: 4821 2815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3973 4298 4684 4898 5200 5581 3973
Size: 325 386 214 302 381 6028
Fragments arranged by size:
6028 386 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 5926 5980 7466 7631 50
Size: 5876 54 1486 165 55
Fragments arranged by size:
5876 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1178 1849 3142 4289 5546 1178
Size: 671 1293 1147 1257 3268
Fragments arranged by size:
3268 1293 1257 1147 671
BsrFI r'CCGG_y
Cuts at: 5632 5813 6024 5632
Size: 181 211 7244
Fragments arranged by size:
7244 211 181
BssSI C'ACGA_G
Cuts at: 5905 6265 5905
Size: 360 7276
BstAPI GCAn_nnn'nTGC
Cuts at: 522 5011 522
Size: 4489 3147
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7636
BstEII G'GTnAC_C
Cuts at: 237 237
Size: 7636
BstXI CCAn_nnnn'nTGG
Cuts at: 1704 2673 1704
Size: 969 6667
BstYI r'GATC_y
Cuts at: 1 420 661 996 1164 1635 2640 2751 3339
Size: 419 241 335 168 471 1005 111 588
Cuts at: 3339 3975 4659 5126 5149 5484 5730 6744 6830
Size: 636 684 467 23 335 246 1014 86
Cuts at: 6830 6842 7188 1
Size: 12 346 449
Fragments arranged by size:
1014 1005 684 636 588 471 467 449
419 346 335 335 246 241 168 111
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1099 1234 2102 1099
Size: 135 868 6633
Fragments arranged by size:
6633 868 135
BtgI C'CryG_G
Cuts at: 153 737 3861 153
Size: 584 3124 3928
Fragments arranged by size:
3928 3124 584
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 5584 5765 5928 1891
Size: 3693 181 163 3599
Fragments arranged by size:
3693 3599 181 163
BtsI GCAGTG_nn'
Cuts at: 377 593 2509 3127 3622 4877 377
Size: 216 1916 618 495 1255 3136
Fragments arranged by size:
3136 1916 1255 618 495 216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1040 1075 2003 2038 1040
Size: 35 928 35 6638
Fragments arranged by size:
6638 928 35 35
DraI TTT'AAA
Cuts at: 1536 2364 3377 4633 1536
Size: 828 1013 1256 4539
Fragments arranged by size:
4539 1256 1013 828
DraIII CAC_nnn'GTG
Cuts at: 2083 2083
Size: 7636
DrdI GACnn_nn'nnGTC
Cuts at: 2797 5340 6200 2797
Size: 2543 860 4233
Fragments arranged by size:
4233 2543 860
EaeI y'GGCC_r
Cuts at: 1437 2847 3858 5219 5393 5784 5811 7158 1437
Size: 1410 1011 1361 174 391 27 1347 1915
Fragments arranged by size:
1915 1410 1361 1347 1011 391 174 27
EagI C'GGCC_G
Cuts at: 5219 5219
Size: 7636
EarI CTCTTCn'nnn_
Cuts at: 623 857 974 1556 2392 2467 3944 5657 5867
Size: 234 117 582 836 75 1477 1713 210
Cuts at: 5867 623
Size: 2392
Fragments arranged by size:
2392 1713 1477 836 582 234 210 117
75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2925 3714 5842 6154 6300 7446 2925
Size: 789 2128 312 146 1146 3115
Fragments arranged by size:
3115 2128 1146 789 312 146
EcoICRI GAG'CTC
Cuts at: 4646 4646
Size: 7636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 1905 2251 2416 2491 2843 3088 3176 3419
Size: 490 346 165 75 352 245 88 243
Cuts at: 3419 3455 4405 5459 5891 6640 6908 1415
Size: 36 950 1054 432 749 268 2143
Fragments arranged by size:
2143 1054 950 749 490 432 352 346
268 245 243 165 88 75 36
EcoNI CCTnn'n_nnAGG
Cuts at: 460 954 460
Size: 494 7142
EcoO109I rG'GnC_Cy
Cuts at: 465 3734 4773 465
Size: 3269 1039 3328
Fragments arranged by size:
3328 3269 1039
EcoRI G'AATT_C
Cuts at: 4349 4638 4349
Size: 289 7347
EcoRV GAT'ATC
Cuts at: 309 309
Size: 7636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5653 5653
Size: 7636
FspI TGC'GCA
Cuts at: 5415 5415
Size: 7636
HaeII r_GCGC'y
Cuts at: 3642 5068 5316 6340 7319 3642
Size: 1426 248 1024 979 3959
Fragments arranged by size:
3959 1426 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 282 289 314 400 432 628 660 920
Size: 25 7 25 86 32 196 32 260
Cuts at: 920 952 1340 1372 2235 2267 2961 2993 3099
Size: 32 388 32 863 32 694 32 106
Cuts at: 3099 3131 3430 3462 3820 3852 5476 5506 5508
Size: 32 299 32 358 32 1624 30 2
Cuts at: 5508 5538 257
Size: 30 2355
Fragments arranged by size:
2355 1624 863 694 388 358 299 260
196 106 86 32 32 32 32 32
32 32 32 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 962 1025 1289 1365 4673 962
Size: 63 264 76 3308 3925
Fragments arranged by size:
3925 3308 264 76 63
HindIII A'AGCT_T
Cuts at: 3022 3371 3022
Size: 349 7287
HpaI GTT'AAC
Cuts at: 962 1289 962
Size: 327 7309
KasI G'GCGC_C
Cuts at: 3638 5064 5312 3638
Size: 1426 248 5962
Fragments arranged by size:
5962 1426 248
KpnI G_GTAC'C
Cuts at: 950 4654 950
Size: 3704 3932
MfeI C'AATT_G
Cuts at: 470 470
Size: 7636
MluI A'CGCG_T
Cuts at: 7069 7069
Size: 7636
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1024 4475 6307 6491 1024
Size: 3451 1832 184 2169
Fragments arranged by size:
3451 2169 1832 184
MscI TGG'CCA
Cuts at: 1439 5395 1439
Size: 3956 3680
MslI CAynn'nnrTG
Cuts at: 1148 4261 5623 5750 1148
Size: 3113 1362 127 3034
Fragments arranged by size:
3113 3034 1362 127
MspA1I CmG'CkG
Cuts at: 1461 2384 2469 4459 4745 5059 5419 6434 6679
Size: 923 85 1990 286 314 360 1015 245
Cuts at: 6679 1461
Size: 2418
Fragments arranged by size:
2418 1990 1015 923 360 314 286 245
85
NaeI GCC'GGC
Cuts at: 5815 5815
Size: 7636
NarI GG'CG_CC
Cuts at: 3639 5065 5313 3639
Size: 1426 248 5962
Fragments arranged by size:
5962 1426 248
NcoI C'CATG_G
Cuts at: 153 737 153
Size: 584 7052
NdeI CA'TA_TG
Cuts at: 4007 4007
Size: 7636
NgoMIV G'CCGG_C
Cuts at: 5813 5813
Size: 7636
NruI TCG'CGA
Cuts at: 5997 5997
Size: 7636
NsiI A_TGCA'T
Cuts at: 971 3456 971
Size: 2485 5151
NspI r_CATG'y
Cuts at: 852 4687 5187 6096 852
Size: 3835 500 909 2392
Fragments arranged by size:
3835 2392 909 500
PciI A'CATG_T
Cuts at: 6092 6092
Size: 7636
PflMI CCAn_nnn'nTGG
Cuts at: 3352 3352
Size: 7636
PmeI GTTT'AAAC
Cuts at: 4633 4633
Size: 7636
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6775 6807 6775
Size: 32 7604
PpuMI rG'GwC_Cy
Cuts at: 465 3734 465
Size: 3269 4367
PstI C_TGCA'G
Cuts at: 195 3122 3984 4681 5366 195
Size: 2927 862 697 685 2465
Fragments arranged by size:
2927 2465 862 697 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7636
PvuII CAG'CTG
Cuts at: 1461 2384 2469 4459 5059 5419 1461
Size: 923 85 1990 600 360 3678
Fragments arranged by size:
3678 1990 923 600 360 85
RsrII CG'GwC_CG
Cuts at: 5829 5829
Size: 7636
SacI G_AGCT'C
Cuts at: 4648 4648
Size: 7636
SalI G'TCGA_C
Cuts at: 4671 4671
Size: 7636
SapI GCTCTTCn'nnn_
Cuts at: 623 974 5657 5867 623
Size: 351 4683 210 2392
Fragments arranged by size:
4683 2392 351 210
SbfI CC_TGCA'GG
Cuts at: 3984 4681 3984
Size: 697 6939
ScaI AGT'ACT
Cuts at: 4275 7153 4275
Size: 2878 4758
SfcI C'TryA_G
Cuts at: 33 191 1029 1643 2009 2517 3118 3286 3773
Size: 158 838 614 366 508 601 168 487
Cuts at: 3773 3779 3980 4677 5362 6357 6548 33
Size: 6 201 697 685 995 191 1121
Fragments arranged by size:
1121 995 838 697 685 614 601 508
487 366 201 191 168 158 6
SfoI GGC'GCC
Cuts at: 3640 5066 5314 3640
Size: 1426 248 5962
Fragments arranged by size:
5962 1426 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7636
SmaI CCC'GGG
Cuts at: 4656 4656
Size: 7636
SmlI C'TyrA_G
Cuts at: 1799 3882 4789 5704 6198 6460 1799
Size: 2083 907 915 494 262 2975
Fragments arranged by size:
2975 2083 915 907 494 262
SnaBI TAC'GTA
Cuts at: 4626 4626
Size: 7636
SpeI A'CTAG_T
Cuts at: 67 277 67
Size: 210 7426
SphI G_CATG'C
Cuts at: 4687 5187 4687
Size: 500 7136
SspI AAT'ATT
Cuts at: 2074 2074
Size: 7636
StuI AGG'CCT
Cuts at: 4509 4509
Size: 7636
StyI C'CwwG_G
Cuts at: 153 737 3202 3499 3675 4575 4768 5122 153
Size: 584 2465 297 176 900 193 354 2667
Fragments arranged by size:
2667 2465 900 584 354 297 193 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 245 1213 5217 5350 5809 6020 245
Size: 968 4004 133 459 211 1861
Fragments arranged by size:
4004 1861 968 459 211 133
TatI w'GTAC_w
Cuts at: 2938 4273 7151 2938
Size: 1335 2878 3423
Fragments arranged by size:
3423 2878 1335
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 7153 7185 125
Size: 32 6996 32 576
Fragments arranged by size:
6996 576 32 32
Tth111I GACn'n_nGTC
Cuts at: 546 5431 546
Size: 4885 2751
XbaI T'CTAG_A
Cuts at: 4665 4665
Size: 7636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3531 5129 3531
Size: 1598 6038
XmaI C'CCGG_G
Cuts at: 4654 4654
Size: 7636
XmnI GAAnn'nnTTC
Cuts at: 1016 1454 2321 2856 1016
Size: 438 867 535 5796
Fragments arranged by size:
5796 867 535 438
ZraI GAC'GTC
Cuts at: 3875 3875
Size: 7636
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI ApaI AscI BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I ClaI FseI FspAI NheI NotI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI