Restriction map for
pF1KSDA0315
(Circular) (Six-base) MAPSORT of: pF1KSDA0315.seq Check: 8381 from: 1 to: 8653
pF1KSDA0315 8653 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 163 312 967 1238 2474 5088 163
Size: 149 655 271 1236 2614 3728
Fragments arranged by size:
3728 2614 1236 655 271 149
AatII G_ACGT'C
Cuts at: 2756 4723 2756
Size: 1967 6686
AccI GT'mk_AC
Cuts at: 3776 4169 5689 3776
Size: 393 1520 6740
Fragments arranged by size:
6740 1520 393
Acc65I G'GTAC_C
Cuts at: 5667 5667
Size: 8653
AclI AA'CG_TT
Cuts at: 2676 8493 2676
Size: 5817 2836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 3132 6476 6908 7657 7925 999
Size: 2133 3344 432 749 268 1727
Fragments arranged by size:
3344 2133 1727 749 432 268
AfeI AGC'GCT
Cuts at: 3532 3532
Size: 8653
AflIII A'CryG_T
Cuts at: 3260 6003 7109 8086 3260
Size: 2743 1106 977 3827
Fragments arranged by size:
3827 2743 1106 977
AgeI A'CCGG_T
Cuts at: 7041 7041
Size: 8653
AhdI GACnn_n'nnGTC
Cuts at: 4719 4719
Size: 8653
AleI CACnn'nnGTG
Cuts at: 870 1273 1306 3700 5488 870
Size: 403 33 2394 1788 4035
Fragments arranged by size:
4035 2394 1788 403 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 888 1291 2257 3961 5356 5621 888
Size: 403 966 1704 1395 265 3920
Fragments arranged by size:
3920 1704 1395 966 403 265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1492 1524 1492
Size: 32 8621
AlwNI CAG_nnn'CTG
Cuts at: 423 1652 2133 2423 4701 4845 5956 7525 423
Size: 1229 481 290 2278 144 1111 1569 1551
Fragments arranged by size:
2278 1569 1551 1229 1111 481 290 144
ApaI G_GGCC'C
Cuts at: 147 337 1046 1725 2566 4432 147
Size: 190 709 679 841 1866 4368
Fragments arranged by size:
4368 1866 841 709 679 190
ApaLI G'TGCA_C
Cuts at: 357 1081 3925 7423 357
Size: 724 2844 3498 1587
Fragments arranged by size:
3498 2844 1587 724
ApoI r'AATT_y
Cuts at: 3880 5655 8006 8262 3880
Size: 1775 2351 256 4271
Fragments arranged by size:
4271 2351 1775 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8653
AvaI C'yCGr_G
Cuts at: 1127 3467 3637 5459 5671 1127
Size: 2340 170 1822 212 4109
Fragments arranged by size:
4109 2340 1822 212 170
BamHI G'GATC_C
Cuts at: 1367 2546 5399 5559 5676 1367
Size: 1179 2853 160 117 4344
Fragments arranged by size:
4344 2853 1179 160 117
BanI G'GyrC_C
Cuts at: 175 955 1123 1351 1396 2854 2875 3356 3472
Size: 780 168 228 45 1458 21 481 116
Cuts at: 3472 3523 4892 5058 5073 5667 6081 6329 6364
Size: 51 1369 166 15 594 414 248 35
Cuts at: 6364 175
Size: 2464
Fragments arranged by size:
2464 1458 1369 780 594 481 414 248
228 168 166 116 51 45 35 21
15
BanII G_rGCy'C
Cuts at: 147 337 601 1046 1198 1262 1725 2566 2896
Size: 190 264 445 152 64 463 841 330
Cuts at: 2896 3191 3578 4253 4385 4432 4687 5665 6695
Size: 295 387 675 132 47 255 978 1030
Cuts at: 6695 147
Size: 2105
Fragments arranged by size:
2105 1030 978 841 675 463 445 387
330 295 264 255 190 152 132 64
47
BbeI G_GCGC'C
Cuts at: 5062 6085 6333 5062
Size: 1023 248 7382
Fragments arranged by size:
7382 1023 248
BbsI GAAGACnn'nnnn_
Cuts at: 801 810 887 1005 2748 3279 3550 3969 5150
Size: 9 77 118 1743 531 271 419 1181
Cuts at: 5150 5170 801
Size: 20 4284
Fragments arranged by size:
4284 1743 1181 531 419 271 118 77
20 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2352 2386 3480 3514 2352
Size: 34 1094 34 7491
Fragments arranged by size:
7491 1094 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6549 7312 8653 6549
Size: 763 1341 6549
Fragments arranged by size:
6549 1341 763
BclI T'GATC_A
Cuts at: 4016 6171 4016
Size: 2155 6498
BglI GCCn_nnn'nGGC
Cuts at: 167 976 1642 3603 4050 167
Size: 809 666 1961 447 4770
Fragments arranged by size:
4770 1961 809 666 447
BglII A'GATC_T
Cuts at: 4982 5357 6166 8205 4982
Size: 375 809 2039 5430
Fragments arranged by size:
5430 2039 809 375
BlpI GC'TnA_GC
Cuts at: 3718 5314 5763 3718
Size: 1596 449 6608
Fragments arranged by size:
6608 1596 449
Bme1580I G_kGCm'C
Cuts at: 147 337 361 1046 1085 1354 1725 2106 2527
Size: 190 24 685 39 269 371 381 421
Cuts at: 2527 2566 3038 3359 3366 3475 3749 3929 4432
Size: 39 472 321 7 109 274 180 503
Cuts at: 4432 5017 5069 6276 6369 7427 147
Size: 585 52 1207 93 1058 1373
Fragments arranged by size:
1373 1207 1058 685 585 503 472 421
381 371 321 274 269 190 180 109
93 52 39 39 24 7
BmgBI CAC'GTC
Cuts at: 1943 2907 3711 3924 4011 5229 1943
Size: 964 804 213 87 1218 5367
Fragments arranged by size:
5367 1218 964 804 213 87
BmrI ACTGGGnnnn_n'
Cuts at: 1548 2121 2776 5863 5944 6279 8425 1548
Size: 573 655 3087 81 335 2146 1776
Fragments arranged by size:
3087 2146 1776 655 573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1431 4807 7018 1431
Size: 3376 2211 3066
Fragments arranged by size:
3376 3066 2211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 925 1182 1487 2008 2947 3028 3807 3859
Size: 591 257 305 521 939 81 779 52
Cuts at: 3859 3868 4153 4231 4267 4276 4690 4891 5638
Size: 9 285 78 36 9 414 201 747
Cuts at: 5638 334
Size: 3349
Fragments arranged by size:
3349 939 779 747 591 521 414 305
285 257 201 81 78 52 36 9
9
Bpu10I CC'TnA_GC
Cuts at: 499 1534 1765 4040 499
Size: 1035 231 2275 5112
Fragments arranged by size:
5112 2275 1035 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2230 4348 5188 5827 6706 7200 7498 1357
Size: 873 2118 840 639 879 494 298 2512
Fragments arranged by size:
2512 2118 879 873 840 639 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1463 3018 3550 4818 8357 1463
Size: 1555 532 1268 3539 1759
Fragments arranged by size:
3539 1759 1555 1268 532
BsaAI yAC'GTr
Cuts at: 1893 2229 3261 3399 4266 5643 6004 1893
Size: 336 1032 138 867 1377 361 4542
Fragments arranged by size:
4542 1377 1032 867 361 336 138
BsaBI GATnn'nnATC
Cuts at: 6 4342 6165 8204 6
Size: 4336 1823 2039 455
Fragments arranged by size:
4336 2039 1823 455
BsaHI Gr'CG_yC
Cuts at: 2612 2725 2753 2927 3434 4550 4677 4720 5059
Size: 113 28 174 507 1116 127 43 339
Cuts at: 5059 5252 6082 6330 7034 8530 2612
Size: 193 830 248 704 1496 2735
Fragments arranged by size:
2735 1496 1116 830 704 507 339 248
193 174 127 113 43 28
BsaWI w'CCGG_w
Cuts at: 692 5183 5840 6361 7041 7315 7462 7873 692
Size: 4491 657 521 680 274 147 411 1472
Fragments arranged by size:
4491 1472 680 657 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 880 910 1765 1795 2389 2419 2500 2530 2878
Size: 30 855 30 594 30 81 30 348
Cuts at: 2878 2908 4066 4096 4372 4402 4570 4600 4723
Size: 30 1158 30 276 30 168 30 123
Cuts at: 4723 4753 5458 5488 880
Size: 30 705 30 4045
Fragments arranged by size:
4045 1158 855 705 594 348 276 168
123 81 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1392 1654 1786 1903 2005 3325 3865 4888 4891
Size: 262 132 117 102 1320 540 1023 3
Cuts at: 4891 5569 1392
Size: 678 4476
Fragments arranged by size:
4476 1320 1023 678 540 262 132 117
102 3
BseYI C'CCAG_C
Cuts at: 156 165 1242 1697 2140 3675 3833 4485 4817
Size: 9 1077 455 443 1535 158 652 332
Cuts at: 4817 5329 6076 6834 7413 7909 156
Size: 512 747 758 579 496 900
Fragments arranged by size:
1535 1077 900 758 747 652 579 512
496 455 443 332 158 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 1533 1543 1756 2209 2323 3519 3607 4183
Size: 639 10 213 453 114 1196 88 576
Cuts at: 4183 4552 5277 5434 5597 894
Size: 369 725 157 163 3950
Fragments arranged by size:
3950 1196 725 639 576 453 369 213
163 157 114 88 10
BsiEI CG_ry'CG
Cuts at: 104 1307 1316 2652 3972 4054 4906 4910 6193
Size: 1203 9 1336 1320 82 852 4 1283
Cuts at: 6193 6239 7449 104
Size: 46 1210 1308
Fragments arranged by size:
1336 1320 1308 1283 1210 1203 852 82
46 9 4
BsiHKAI G_wGCw'C
Cuts at: 361 601 679 1085 1229 3929 4253 5665 6443
Size: 240 78 406 144 2700 324 1412 778
Cuts at: 6443 6633 7427 361
Size: 190 794 1587
Fragments arranged by size:
2700 1587 1412 794 778 406 324 240
190 144 78
BsmI GAATG_Cn'
Cuts at: 285 285
Size: 8653
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2551 2716 2320
Size: 231 165 8257
Fragments arranged by size:
8257 231 165
Bsp1286I G_dGCh'C
Cuts at: 147 337 361 601 679 1046 1085 1198 1229
Size: 190 24 240 78 367 39 113 31
Cuts at: 1229 1262 1354 1725 2106 2527 2566 2896 3038
Size: 33 92 371 381 421 39 330 142
Cuts at: 3038 3191 3359 3366 3475 3578 3749 3929 4253
Size: 153 168 7 109 103 171 180 324
Cuts at: 4253 4385 4432 4687 5017 5069 5665 6276 6369
Size: 132 47 255 330 52 596 611 93
Cuts at: 6369 6443 6633 6695 7427 147
Size: 74 190 62 732 1373
Fragments arranged by size:
1373 732 611 596 421 381 371 367
330 330 324 255 240 190 190 180
171 168 153 142 132 113 109 103
93 92 78 74 62 52 47 39
39 33 31 24 7
BspEI T'CCGG_A
Cuts at: 7873 7873
Size: 8653
BspHI T'CATG_A
Cuts at: 7829 7829
Size: 8653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 163 312 923 967 1238 1555 2006 2041 2054
Size: 149 611 44 271 317 451 35 13
Cuts at: 2054 2474 3435 3641 4097 4289 4853 5088 5406
Size: 420 961 206 456 192 564 235 318
Cuts at: 5406 5701 5915 6217 6598 163
Size: 295 214 302 381 2218
Fragments arranged by size:
2218 961 611 564 456 451 420 381
318 317 302 295 271 235 214 206
192 149 44 35 13
BsrBI CCG'CTC
Cuts at: 50 3717 4962 5181 6943 6997 8483 8648 50
Size: 3667 1245 219 1762 54 1486 165 55
Fragments arranged by size:
3667 1762 1486 1245 219 165 55 54
BsrDI GCAATG_nn'
Cuts at: 570 651 6563 570
Size: 81 5912 2660
Fragments arranged by size:
5912 2660 81
BsrFI r'CCGG_y
Cuts at: 748 1518 1715 2259 2647 3934 4021 4053 4736
Size: 770 197 544 388 1287 87 32 683
Cuts at: 4736 6649 6830 7041 748
Size: 1913 181 211 2360
Fragments arranged by size:
2360 1913 1287 770 683 544 388 211
197 181 87 32
BsrGI T'GTAC_A
Cuts at: 4388 4388
Size: 8653
BssHII G'CGCG_C
Cuts at: 492 492
Size: 8653
BssSI C'ACGA_G
Cuts at: 2056 2167 3928 5239 5488 6922 7282 2056
Size: 111 1761 1311 249 1434 360 3427
Fragments arranged by size:
3427 1761 1434 1311 360 249 111
BstAPI GCAn_nnn'nTGC
Cuts at: 1556 1676 2012 2423 2432 4845 4899 5073 5412
Size: 120 336 411 9 2413 54 174 339
Cuts at: 5412 6028 1556
Size: 616 4181
Fragments arranged by size:
4181 2413 616 411 339 336 174 120
54 9
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8653
BstEII G'GTnAC_C
Cuts at: 616 2262 2913 616
Size: 1646 651 6356
Fragments arranged by size:
6356 1646 651
BstXI CCAn_nnnn'nTGG
Cuts at: 1986 2148 3243 1986
Size: 162 1095 7396
Fragments arranged by size:
7396 1095 162
BstYI r'GATC_y
Cuts at: 1 1367 2546 2636 4697 4982 5357 5399 5559
Size: 1366 1179 90 2061 285 375 42 160
Cuts at: 5559 5676 6143 6166 6501 6747 7761 7847 7859
Size: 117 467 23 335 246 1014 86 12
Cuts at: 7859 8205 1
Size: 346 449
Fragments arranged by size:
2061 1366 1179 1014 467 449 375 346
335 285 246 160 117 90 86 42
23 12
Bsu36I CC'TnA_GG
Cuts at: 184 252 2084 3308 184
Size: 68 1832 1224 5529
Fragments arranged by size:
5529 1832 1224 68
BtgI C'CryG_G
Cuts at: 125 329 602 974 1161 1630 2871 3170 3211
Size: 204 273 372 187 469 1241 299 41
Cuts at: 3211 3749 4045 4623 125
Size: 538 296 578 4155
Fragments arranged by size:
4155 1241 578 538 469 372 299 296
273 204 187 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3165 3505 3747 5253 6601 6782 6945 3165
Size: 340 242 1506 1348 181 163 4873
Fragments arranged by size:
4873 1506 1348 340 242 181 163
BtsI GCAGTG_nn'
Cuts at: 130 925 1041 2241 5017 5894 130
Size: 795 116 1200 2776 877 2889
Fragments arranged by size:
2889 2776 1200 877 795 116
DraI TTT'AAA
Cuts at: 5650 5650
Size: 8653
DraIII CAC_nnn'GTG
Cuts at: 1556 4899 5073 5244 1556
Size: 3343 174 171 4965
Fragments arranged by size:
4965 3343 174 171
DrdI GACnn_nn'nnGTC
Cuts at: 2095 3280 4663 5233 6357 7217 2095
Size: 1185 1383 570 1124 860 3531
Fragments arranged by size:
3531 1383 1185 1124 860 570
EaeI y'GGCC_r
Cuts at: 326 557 578 965 1182 1304 1362 1682 1895
Size: 231 21 387 217 122 58 320 213
Cuts at: 1895 2412 2645 2649 3117 4051 4271 4903 6236
Size: 517 233 4 468 934 220 632 1333
Cuts at: 6236 6410 6801 6828 8175 326
Size: 174 391 27 1347 804
Fragments arranged by size:
1347 1333 934 804 632 517 468 391
387 320 233 231 220 217 213 174
122 58 27 21 4
EagI C'GGCC_G
Cuts at: 1304 2649 4051 4903 6236 1304
Size: 1345 1402 852 1333 3721
Fragments arranged by size:
3721 1402 1345 1333 852
EarI CTCTTCn'nnn_
Cuts at: 475 3787 4246 4402 6674 6884 475
Size: 3312 459 156 2272 210 2244
Fragments arranged by size:
3312 2272 2244 459 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 505 5581 6859 7171 7317 8463 505
Size: 5076 1278 312 146 1146 695
Fragments arranged by size:
5076 1278 1146 695 312 146
EcoICRI GAG'CTC
Cuts at: 599 4251 5663 599
Size: 3652 1412 3589
Fragments arranged by size:
3652 3589 1412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 925 999 1182 1487 2008 2947 3028 3132
Size: 591 74 183 305 521 939 81 104
Cuts at: 3132 3807 3859 3868 4153 4231 4267 4276 4690
Size: 675 52 9 285 78 36 9 414
Cuts at: 4690 4891 5638 6476 6908 7657 7925 334
Size: 201 747 838 432 749 268 1062
Fragments arranged by size:
1062 939 838 749 747 675 591 521
432 414 305 285 268 201 183 104
81 78 74 52 36 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 472 1768 4801 4870 4873 472
Size: 1296 3033 69 3 4252
Fragments arranged by size:
4252 3033 1296 69 3
EcoO109I rG'GnC_Cy
Cuts at: 144 188 607 927 1042 1572 2043 2137 2282
Size: 44 419 320 115 530 471 94 145
Cuts at: 2282 2499 2563 3026 3386 3843 4068 4428 4566
Size: 217 64 463 360 457 225 360 138
Cuts at: 4566 4842 5790 144
Size: 276 948 3007
Fragments arranged by size:
3007 948 530 471 463 457 419 360
360 320 276 225 217 145 138 115
94 64 44
EcoRI G'AATT_C
Cuts at: 3880 5655 3880
Size: 1775 6878
EcoRV GAT'ATC
Cuts at: 1170 1170
Size: 8653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 3303 4188 5112 6670 219
Size: 3084 885 924 1558 2202
Fragments arranged by size:
3084 2202 1558 924 885
FseI GG_CCGG'CC
Cuts at: 2651 2651
Size: 8653
FspI TGC'GCA
Cuts at: 4297 6432 4297
Size: 2135 6518
HaeII r_GCGC'y
Cuts at: 450 1697 3534 4198 4860 5062 6085 6333 7357
Size: 1247 1837 664 662 202 1023 248 1024
Cuts at: 7357 8336 450
Size: 979 767
Fragments arranged by size:
1837 1247 1024 1023 979 767 664 662
248 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 1384 1416 1916 1948 2878 2910 3208
Size: 32 1117 32 500 32 930 32 298
Cuts at: 3208 3240 4570 4602 4705 4737 4915 4947 6493
Size: 32 1330 32 103 32 178 32 1546
Cuts at: 6493 6523 6525 6555 235
Size: 30 2 30 2333
Fragments arranged by size:
2333 1546 1330 1117 930 500 298 178
103 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 414 3450 4752 5690 414
Size: 3036 1302 938 3377
Fragments arranged by size:
3377 3036 1302 938
HindIII A'AGCT_T
Cuts at: 3102 3102
Size: 8653
KasI G'GCGC_C
Cuts at: 5058 6081 6329 5058
Size: 1023 248 7382
Fragments arranged by size:
7382 1023 248
KpnI G_GTAC'C
Cuts at: 5671 5671
Size: 8653
MluI A'CGCG_T
Cuts at: 8086 8086
Size: 8653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 2826 4109 4355 4685 7324 7508 668
Size: 2158 1283 246 330 2639 184 1813
Fragments arranged by size:
2639 2158 1813 1283 330 246 184
MscI TGG'CCA
Cuts at: 328 559 580 4273 6412 328
Size: 231 21 3693 2139 2569
Fragments arranged by size:
3693 2569 2139 231 21
MslI CAynn'nnrTG
Cuts at: 670 870 1273 1306 3241 3700 3766 3943 4558
Size: 200 403 33 1935 459 66 177 615
Cuts at: 4558 4759 4769 5488 6640 6767 670
Size: 201 10 719 1152 127 2556
Fragments arranged by size:
2556 1935 1152 719 615 459 403 201
200 177 127 66 33 10
MspA1I CmG'CkG
Cuts at: 294 312 420 539 946 1071 1242 1632 1740
Size: 18 108 119 407 125 171 390 108
Cuts at: 1740 2027 2120 3213 3735 3837 3869 4047 4143
Size: 287 93 1093 522 102 32 178 96
Cuts at: 4143 4728 5583 5611 5762 6076 6436 7451 7696
Size: 585 855 28 151 314 360 1015 245
Cuts at: 7696 294
Size: 1251
Fragments arranged by size:
1251 1093 1015 855 585 522 407 390
360 314 287 245 178 171 151 125
119 108 108 102 96 93 32 28
18
NaeI GCC'GGC
Cuts at: 750 1717 2649 3936 6832 750
Size: 967 932 1287 2896 2571
Fragments arranged by size:
2896 2571 1287 967 932
NarI GG'CG_CC
Cuts at: 5059 6082 6330 5059
Size: 1023 248 7382
Fragments arranged by size:
7382 1023 248
NcoI C'CATG_G
Cuts at: 125 3170 3749 125
Size: 3045 579 5029
Fragments arranged by size:
5029 3045 579
NgoMIV G'CCGG_C
Cuts at: 748 1715 2647 3934 6830 748
Size: 967 932 1287 2896 2571
Fragments arranged by size:
2896 2571 1287 967 932
NruI TCG'CGA
Cuts at: 3665 7014 3665
Size: 3349 5304
NspI r_CATG'y
Cuts at: 873 5236 5704 6204 7113 873
Size: 4363 468 500 909 2413
Fragments arranged by size:
4363 2413 909 500 468
PasI CC'CwG_GG
Cuts at: 1725 2568 1725
Size: 843 7810
PciI A'CATG_T
Cuts at: 7109 7109
Size: 8653
PflMI CCAn_nnn'nTGG
Cuts at: 1652 2133 4131 5244 1652
Size: 481 1998 1113 5061
Fragments arranged by size:
5061 1998 1113 481
PfoI T'CCnGG_A
Cuts at: 4244 4253 4667 4775 4868 5105 5135 4244
Size: 9 414 108 93 237 30 7762
Fragments arranged by size:
7762 414 237 108 93 30 9
PmeI GTTT'AAAC
Cuts at: 5650 5650
Size: 8653
PmlI CAC'GTG
Cuts at: 1893 2229 1893
Size: 336 8317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7792 7824 7792
Size: 32 8621
PpuMI rG'GwC_Cy
Cuts at: 607 927 1572 2043 2137 2282 3026 4566 4842
Size: 320 645 471 94 145 744 1540 276
Cuts at: 4842 607
Size: 4418
Fragments arranged by size:
4418 1540 744 645 471 320 276 145
94
PshAI GACnn'nnGTC
Cuts at: 2120 2120
Size: 8653
PspOMI G'GGCC_C
Cuts at: 143 333 1042 1721 2562 4428 143
Size: 190 709 679 841 1866 4368
Fragments arranged by size:
4368 1866 841 709 679 190
PstI C_TGCA'G
Cuts at: 137 311 920 1283 2428 3197 3233 3503 5024
Size: 174 609 363 1145 769 36 270 1521
Cuts at: 5024 5085 5600 5698 6383 137
Size: 61 515 98 685 2407
Fragments arranged by size:
2407 1521 1145 769 685 609 515 363
270 174 98 61 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8653
PvuII CAG'CTG
Cuts at: 294 312 420 1740 2120 3837 5583 6076 6436
Size: 18 108 1320 380 1717 1746 493 360
Cuts at: 6436 294
Size: 2511
Fragments arranged by size:
2511 1746 1717 1320 493 380 360 108
18
RsrII CG'GwC_CG
Cuts at: 6846 6846
Size: 8653
SacI G_AGCT'C
Cuts at: 601 4253 5665 601
Size: 3652 1412 3589
Fragments arranged by size:
3652 3589 1412
SacII CC_GC'GG
Cuts at: 1633 3214 4048 1633
Size: 1581 834 6238
Fragments arranged by size:
6238 1581 834
SalI G'TCGA_C
Cuts at: 5688 5688
Size: 8653
SanDI GG'GwC_CC
Cuts at: 927 2137 3026 927
Size: 1210 889 6554
Fragments arranged by size:
6554 1210 889
SapI GCTCTTCn'nnn_
Cuts at: 3787 4246 4402 6674 6884 3787
Size: 459 156 2272 210 5556
Fragments arranged by size:
5556 2272 459 210 156
SbfI CC_TGCA'GG
Cuts at: 5698 5698
Size: 8653
ScaI AGT'ACT
Cuts at: 4216 5092 5527 8170 4216
Size: 876 435 2643 4699
Fragments arranged by size:
4699 2643 876 435
SexAI A'CCwGG_T
Cuts at: 1454 4613 4895 1454
Size: 3159 282 5212
Fragments arranged by size:
5212 3159 282
SfcI C'TryA_G
Cuts at: 33 133 307 916 1279 2424 2454 3193 3229
Size: 100 174 609 363 1145 30 739 36
Cuts at: 3229 3499 5020 5081 5596 5694 6379 7374 7565
Size: 270 1521 61 515 98 685 995 191
Cuts at: 7565 33
Size: 1121
Fragments arranged by size:
1521 1145 1121 995 739 685 609 515
363 270 191 174 100 98 61 36
30
SfiI GGCCn_nnn'nGGCC
Cuts at: 1642 1642
Size: 8653
SfoI GGC'GCC
Cuts at: 5060 6083 6331 5060
Size: 1023 248 7382
Fragments arranged by size:
7382 1023 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8653
SmaI CCC'GGG
Cuts at: 1129 3469 5461 5673 1129
Size: 2340 1992 212 4109
Fragments arranged by size:
4109 2340 1992 212
SmlI C'TyrA_G
Cuts at: 1372 2245 4363 5203 5806 6721 7215 7477 1372
Size: 873 2118 840 603 915 494 262 2548
Fragments arranged by size:
2548 2118 915 873 840 603 494 262
SnaBI TAC'GTA
Cuts at: 5643 5643
Size: 8653
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8653
SphI G_CATG'C
Cuts at: 5704 6204 5704
Size: 500 8153
StuI AGG'CCT
Cuts at: 250 712 1292 3322 3514 250
Size: 462 580 2030 192 5389
Fragments arranged by size:
5389 2030 580 462 192
StyI C'CwwG_G
Cuts at: 125 3170 3278 3749 3992 4163 4802 5351 5549
Size: 3045 108 471 243 171 639 549 198
Cuts at: 5549 5785 6139 125
Size: 236 354 2639
Fragments arranged by size:
3045 2639 639 549 471 354 243 236
198 171 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1567 2560 2893 3511 3574 3660 4924 5181 6234
Size: 993 333 618 63 86 1264 257 1053
Cuts at: 6234 6367 6826 7037 1567
Size: 133 459 211 3183
Fragments arranged by size:
3183 1264 1053 993 618 459 333 257
211 133 86 63
TatI w'GTAC_w
Cuts at: 3566 4214 4388 4460 5090 5525 8168 3566
Size: 648 174 72 630 435 2643 4051
Fragments arranged by size:
4051 2643 648 630 435 174 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 904 936 2688 2720 2852 2884 4723 4755 5458
Size: 32 1752 32 132 32 1839 32 703
Cuts at: 5458 5490 8170 8202 904
Size: 32 2680 32 1355
Fragments arranged by size:
2680 1839 1752 1355 703 132 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 409 3973 6448 409
Size: 3564 2475 2614
Fragments arranged by size:
3564 2614 2475
XbaI T'CTAG_A
Cuts at: 5682 5682
Size: 8653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 588 2971 3243 4621 6146 447
Size: 141 2383 272 1378 1525 2954
Fragments arranged by size:
2954 2383 1525 1378 272 141
XmaI C'CCGG_G
Cuts at: 1127 3467 5459 5671 1127
Size: 2340 1992 212 4109
Fragments arranged by size:
4109 2340 1992 212
XmnI GAAnn'nnTTC
Cuts at: 550 3880 550
Size: 3330 5323
ZraI GAC'GTC
Cuts at: 2754 4721 2754
Size: 1967 6686
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BbvCI BfrBI BmtI BsiWI
BstZ17I ClaI CspCI FspAI HpaI MfeI NdeI NheI
NotI NsiI PacI PsiI PspXI PsrI SgrAI SrfI
SspI SwaI XhoI