Restriction map for
pF1KSDA0317
(Circular) (Six-base) MAPSORT of: pF1KSDA0317.seq Check: 9861 from: 1 to: 5608
pF1KSDA0317 5608 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1073 1073
Size: 5608
AccI GT'mk_AC
Cuts at: 2644 2644
Size: 5608
Acc65I G'GTAC_C
Cuts at: 2622 2622
Size: 5608
AclI AA'CG_TT
Cuts at: 5448 5448
Size: 5608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 980 1252 1537 2365 3431 3863 4612 4880
Size: 517 272 285 828 1066 432 749 268
Cuts at: 4880 463
Size: 1191
Fragments arranged by size:
1191 1066 828 749 517 432 285 272
268
AflIII A'CryG_T
Cuts at: 207 2491 2958 4064 5041 207
Size: 2284 467 1106 977 774
Fragments arranged by size:
2284 1106 977 774 467
AgeI A'CCGG_T
Cuts at: 3996 3996
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1327 1327
Size: 5608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 407 439 599 631 407
Size: 32 160 32 5384
Fragments arranged by size:
5384 160 32 32
AlwNI CAG_nnn'CTG
Cuts at: 157 2911 4480 157
Size: 2754 1569 1285
Fragments arranged by size:
2754 1569 1285
ApaLI G'TGCA_C
Cuts at: 2539 4378 2539
Size: 1839 3769
ApoI r'AATT_y
Cuts at: 455 593 934 1282 1566 1936 2106 2610 4961
Size: 138 341 348 284 370 170 504 2351
Cuts at: 4961 5217 455
Size: 256 846
Fragments arranged by size:
2351 846 504 370 348 341 284 256
170 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 5608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5608
AvaI C'yCGr_G
Cuts at: 698 878 2626 698
Size: 180 1748 3680
Fragments arranged by size:
3680 1748 180
BamHI G'GATC_C
Cuts at: 291 2631 291
Size: 2340 3268
BanI G'GyrC_C
Cuts at: 1061 1247 2622 3036 3284 3319 1061
Size: 186 1375 414 248 35 3350
Fragments arranged by size:
3350 1375 414 248 186 35
BanII G_rGCy'C
Cuts at: 488 779 1364 1860 2067 2620 3650 488
Size: 291 585 496 207 553 1030 2446
Fragments arranged by size:
2446 1030 585 553 496 291 207
BbeI G_GCGC'C
Cuts at: 1251 3040 3288 1251
Size: 1789 248 3571
Fragments arranged by size:
3571 1789 248
BbsI GAAGACnn'nnnn_
Cuts at: 1136 1136
Size: 5608
BciVI GTATCCnnnnn_n'
Cuts at: 3504 4267 5608 3504
Size: 763 1341 3504
Fragments arranged by size:
3504 1341 763
BclI T'GATC_A
Cuts at: 3126 3126
Size: 5608
BfrBI ATG'CAT
Cuts at: 1905 1905
Size: 5608
BglII A'GATC_T
Cuts at: 3121 5160 3121
Size: 2039 3569
BlpI GC'TnA_GC
Cuts at: 1363 2718 1363
Size: 1355 4253
Bme1580I G_kGCm'C
Cuts at: 2543 3231 3324 4382 2543
Size: 688 93 1058 3769
Fragments arranged by size:
3769 1058 688 93
BmrI ACTGGGnnnn_n'
Cuts at: 1641 2818 2899 3234 5380 1641
Size: 1177 81 335 2146 1869
Fragments arranged by size:
2146 1869 1177 335 81
BmtI G_CTAG'C
Cuts at: 448 448
Size: 5608
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3973 3973
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 2057 2267 2435 208
Size: 1849 210 168 3381
Fragments arranged by size:
3381 1849 210 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 770 2245 2782 3661 4155 4453 759
Size: 11 1475 537 879 494 298 1914
Fragments arranged by size:
1914 1475 879 537 494 298 11
BsaI GGTCTCn'nnnn_
Cuts at: 1429 5312 1429
Size: 3883 1725
BsaAI yAC'GTr
Cuts at: 208 273 2598 2959 208
Size: 65 2325 361 2857
Fragments arranged by size:
2857 2325 361 65
BsaBI GATnn'nnATC
Cuts at: 6 3120 5159 6
Size: 3114 2039 455
Fragments arranged by size:
3114 2039 455
BsaHI Gr'CG_yC
Cuts at: 1248 3037 3285 3989 5485 1248
Size: 1789 248 704 1496 1371
Fragments arranged by size:
1789 1496 1371 704 248
BsaWI w'CCGG_w
Cuts at: 254 2795 3316 3996 4270 4417 4828 254
Size: 2541 521 680 274 147 411 1034
Fragments arranged by size:
2541 1034 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1847 2161 2377 2388 1847
Size: 314 216 11 5067
Fragments arranged by size:
5067 314 216 11
BseYI C'CCAG_C
Cuts at: 529 1091 3031 3789 4368 4864 529
Size: 562 1940 758 579 496 1273
Fragments arranged by size:
1940 1273 758 579 562 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 2468 383
Size: 2085 3523
BsiEI CG_ry'CG
Cuts at: 104 3148 3194 4404 104
Size: 3044 46 1210 1308
Fragments arranged by size:
3044 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 752 779 1364 1860 2067 2543 2620 3398 3588
Size: 27 585 496 207 476 77 778 190
Cuts at: 3588 4382 752
Size: 794 1978
Fragments arranged by size:
1978 794 778 585 496 476 207 190
77 27
BsmI GAATG_Cn'
Cuts at: 166 873 1343 166
Size: 707 470 4431
Fragments arranged by size:
4431 707 470
BsmBI CGTCTCn'nnnn_
Cuts at: 1553 1553
Size: 5608
Bsp1286I G_dGCh'C
Cuts at: 488 752 779 1364 1860 2067 2543 2620 3231
Size: 264 27 585 496 207 476 77 611
Cuts at: 3231 3324 3398 3588 3650 4382 488
Size: 93 74 190 62 732 1714
Fragments arranged by size:
1714 732 611 585 496 476 264 207
190 93 77 74 62 27
BspEI T'CCGG_A
Cuts at: 4828 4828
Size: 5608
BspHI T'CATG_A
Cuts at: 771 2051 2321 4784 771
Size: 1280 270 2463 1595
Fragments arranged by size:
2463 1595 1280 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1073 1470 2656 2870 3172 3553 1073
Size: 397 1186 214 302 381 3128
Fragments arranged by size:
3128 1186 397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1406 1759 1871 2458 3898 3952 5438 5603
Size: 1356 353 112 587 1440 54 1486 165
Cuts at: 5603 50
Size: 55
Fragments arranged by size:
1486 1440 1356 587 353 165 112 55
54
BsrDI GCAATG_nn'
Cuts at: 990 3518 990
Size: 2528 3080
BsrFI r'CCGG_y
Cuts at: 1097 3604 3785 3996 1097
Size: 2507 181 211 2709
Fragments arranged by size:
2709 2507 211 181
BssSI C'ACGA_G
Cuts at: 1798 3877 4237 1798
Size: 2079 360 3169
Fragments arranged by size:
3169 2079 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2547 2983 2547
Size: 436 5172
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5608
BstYI r'GATC_y
Cuts at: 1 291 2631 3098 3121 3456 3702 4716 4802
Size: 290 2340 467 23 335 246 1014 86
Cuts at: 4802 4814 5160 1
Size: 12 346 449
Fragments arranged by size:
2340 1014 467 449 346 335 290 246
86 23 12
BtgI C'CryG_G
Cuts at: 1057 1057
Size: 5608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3556 3737 3900 3556
Size: 181 163 5264
Fragments arranged by size:
5264 181 163
BtsI GCAGTG_nn'
Cuts at: 455 1106 2849 455
Size: 651 1743 3214
Fragments arranged by size:
3214 1743 651
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1095 1130 1095
Size: 35 5573
DraI TTT'AAA
Cuts at: 2105 2605 2105
Size: 500 5108
DrdI GACnn_nn'nnGTC
Cuts at: 946 3312 4172 946
Size: 2366 860 2382
Fragments arranged by size:
2382 2366 860
EaeI y'GGCC_r
Cuts at: 1099 2129 2558 3191 3365 3756 3783 5130 1099
Size: 1030 429 633 174 391 27 1347 1577
Fragments arranged by size:
1577 1347 1030 633 429 391 174 27
EagI C'GGCC_G
Cuts at: 3191 3191
Size: 5608
EarI CTCTTCn'nnn_
Cuts at: 788 1092 1705 2000 2528 3629 3839 788
Size: 304 613 295 528 1101 210 2557
Fragments arranged by size:
2557 1101 613 528 304 295 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3814 4126 4272 5418 3814
Size: 312 146 1146 4004
Fragments arranged by size:
4004 1146 312 146
EcoICRI GAG'CTC
Cuts at: 777 1362 1858 2065 2618 777
Size: 585 496 207 553 3767
Fragments arranged by size:
3767 585 553 496 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 463 980 1252 1537 2057 2267 2365 2435
Size: 255 517 272 285 520 210 98 70
Cuts at: 2435 3431 3863 4612 4880 208
Size: 996 432 749 268 936
Fragments arranged by size:
996 936 749 520 517 432 285 272
268 255 210 98 70
EcoNI CCTnn'n_nnAGG
Cuts at: 955 955
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 327 1639 2745 327
Size: 1312 1106 3190
Fragments arranged by size:
3190 1312 1106
EcoRI G'AATT_C
Cuts at: 1936 2610 1936
Size: 674 4934
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 207 1473 3625 207
Size: 1266 2152 2190
Fragments arranged by size:
2190 2152 1266
FspI TGC'GCA
Cuts at: 523 3387 523
Size: 2864 2744
HaeII r_GCGC'y
Cuts at: 246 1098 1251 3040 3288 4312 5291 246
Size: 852 153 1789 248 1024 979 563
Fragments arranged by size:
1789 1024 979 852 563 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 283 315 413 445 1117 1123 1149 1155 2303
Size: 32 98 32 672 6 26 6 1148
Cuts at: 2303 2335 3448 3478 3480 3510 283
Size: 32 1113 30 2 30 2381
Fragments arranged by size:
2381 1148 1113 672 98 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 1116 2645 1116
Size: 1529 4079
HindIII A'AGCT_T
Cuts at: 553 553
Size: 5608
KasI G'GCGC_C
Cuts at: 1247 3036 3284 1247
Size: 1789 248 3571
Fragments arranged by size:
3571 1789 248
KpnI G_GTAC'C
Cuts at: 2626 2626
Size: 5608
MfeI C'AATT_G
Cuts at: 2029 2029
Size: 5608
MluI A'CGCG_T
Cuts at: 5041 5041
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 518 857 1520 2485 4279 4463 518
Size: 339 663 965 1794 184 1663
Fragments arranged by size:
1794 1663 965 663 339 184
MscI TGG'CCA
Cuts at: 2131 2560 3367 2131
Size: 429 807 4372
Fragments arranged by size:
4372 807 429
MslI CAynn'nnrTG
Cuts at: 1078 1327 2257 2490 3595 3722 1078
Size: 249 930 233 1105 127 2964
Fragments arranged by size:
2964 1105 930 249 233 127
MspA1I CmG'CkG
Cuts at: 1367 2505 2556 2717 3031 3391 4406 4651 1367
Size: 1138 51 161 314 360 1015 245 2324
Fragments arranged by size:
2324 1138 1015 360 314 245 161 51
NaeI GCC'GGC
Cuts at: 1099 3787 1099
Size: 2688 2920
NarI GG'CG_CC
Cuts at: 1248 3037 3285 1248
Size: 1789 248 3571
Fragments arranged by size:
3571 1789 248
NcoI C'CATG_G
Cuts at: 1057 1057
Size: 5608
NdeI CA'TA_TG
Cuts at: 1487 2520 1487
Size: 1033 4575
NgoMIV G'CCGG_C
Cuts at: 1097 3785 1097
Size: 2688 2920
NheI G'CTAG_C
Cuts at: 444 444
Size: 5608
NruI TCG'CGA
Cuts at: 3969 3969
Size: 5608
NsiI A_TGCA'T
Cuts at: 1907 1907
Size: 5608
NspI r_CATG'y
Cuts at: 1534 2495 2592 2659 3159 4068 1534
Size: 961 97 67 500 909 3074
Fragments arranged by size:
3074 961 909 500 97 67
PciI A'CATG_T
Cuts at: 2491 4064 2491
Size: 1573 4035
PflMI CCAn_nnn'nTGG
Cuts at: 351 351
Size: 5608
PfoI T'CCnGG_A
Cuts at: 1272 1610 1272
Size: 338 5270
PmeI GTTT'AAAC
Cuts at: 2605 2605
Size: 5608
PmlI CAC'GTG
Cuts at: 208 208
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4747 4779 4747
Size: 32 5576
PpuMI rG'GwC_Cy
Cuts at: 327 327
Size: 5608
PsiI TTA'TAA
Cuts at: 1031 1031
Size: 5608
PstI C_TGCA'G
Cuts at: 2555 2653 3338 2555
Size: 98 685 4825
Fragments arranged by size:
4825 685 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5608
PvuII CAG'CTG
Cuts at: 1367 2505 2556 3031 3391 1367
Size: 1138 51 475 360 3584
Fragments arranged by size:
3584 1138 475 360 51
RsrII CG'GwC_CG
Cuts at: 3801 3801
Size: 5608
SacI G_AGCT'C
Cuts at: 779 1364 1860 2067 2620 779
Size: 585 496 207 553 3767
Fragments arranged by size:
3767 585 553 496 207
SalI G'TCGA_C
Cuts at: 2643 2643
Size: 5608
SapI GCTCTTCn'nnn_
Cuts at: 1705 3629 3839 1705
Size: 1924 210 3474
Fragments arranged by size:
3474 1924 210
SbfI CC_TGCA'GG
Cuts at: 2653 2653
Size: 5608
ScaI AGT'ACT
Cuts at: 801 1915 5125 801
Size: 1114 3210 1284
Fragments arranged by size:
3210 1284 1114
SfcI C'TryA_G
Cuts at: 33 1044 2551 2649 3334 4329 4520 33
Size: 1011 1507 98 685 995 191 1121
Fragments arranged by size:
1507 1121 1011 995 685 191 98
SfoI GGC'GCC
Cuts at: 1249 3038 3286 1249
Size: 1789 248 3571
Fragments arranged by size:
3571 1789 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5608
SmaI CCC'GGG
Cuts at: 2628 2628
Size: 5608
SmlI C'TyrA_G
Cuts at: 738 785 878 2224 2761 3676 4170 4432 738
Size: 47 93 1346 537 915 494 262 1914
Fragments arranged by size:
1914 1346 915 537 494 262 93 47
SnaBI TAC'GTA
Cuts at: 2598 2598
Size: 5608
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5608
SphI G_CATG'C
Cuts at: 2592 2659 3159 2592
Size: 67 500 5041
Fragments arranged by size:
5041 500 67
StuI AGG'CCT
Cuts at: 2171 2171
Size: 5608
StyI C'CwwG_G
Cuts at: 1057 1293 2740 3094 1057
Size: 236 1447 354 3571
Fragments arranged by size:
3571 1447 354 236
SwaI ATTT'AAAT
Cuts at: 2105 2105
Size: 5608
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1086 3189 3322 3781 3992 1086
Size: 2103 133 459 211 2702
Fragments arranged by size:
2702 2103 459 211 133
TatI w'GTAC_w
Cuts at: 799 1184 1913 5123 799
Size: 385 729 3210 1284
Fragments arranged by size:
3210 1284 729 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 137 169 554 586 1784 1816 5125 5157 137
Size: 32 385 32 1198 32 3309 32 588
Fragments arranged by size:
3309 1198 588 385 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1306 3403 1306
Size: 2097 3511
XbaI T'CTAG_A
Cuts at: 2637 2637
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3101 3101
Size: 5608
XhoI C'TCGA_G
Cuts at: 878 878
Size: 5608
XmaI C'CCGG_G
Cuts at: 2626 2626
Size: 5608
XmnI GAAnn'nnTTC
Cuts at: 476 1222 1459 476
Size: 746 237 4625
Fragments arranged by size:
4625 746 237
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI Bpu10I BsaXI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HpaI NotI PacI PasI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI ZraI