Restriction map for
pF1KSDA0323
(Circular) (Six-base) MAPSORT of: pF1KSDA0323.seq Check: 2154 from: 1 to: 5296
pF1KSDA0323 5296 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1341 1341
Size: 5296
AccI GT'mk_AC
Cuts at: 2332 2332
Size: 5296
Acc65I G'GTAC_C
Cuts at: 1971 2310 1971
Size: 339 4957
AclI AA'CG_TT
Cuts at: 5136 5136
Size: 5296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 336 478 644 1689 1865 2041 3119 3551
Size: 182 142 166 1045 176 176 1078 432
Cuts at: 3551 4300 4568 154
Size: 749 268 882
Fragments arranged by size:
1078 1045 882 749 432 268 182 176
176 166 142
AflIII A'CryG_T
Cuts at: 2646 3752 4729 2646
Size: 1106 977 3213
Fragments arranged by size:
3213 1106 977
AgeI A'CCGG_T
Cuts at: 3684 3684
Size: 5296
AlwNI CAG_nnn'CTG
Cuts at: 2175 2599 4168 2175
Size: 424 1569 3303
Fragments arranged by size:
3303 1569 424
ApaI G_GGCC'C
Cuts at: 267 424 267
Size: 157 5139
ApaLI G'TGCA_C
Cuts at: 4066 4066
Size: 5296
ApoI r'AATT_y
Cuts at: 2014 2298 4649 4905 2014
Size: 284 2351 256 2405
Fragments arranged by size:
2405 2351 284 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5296
AvaI C'yCGr_G
Cuts at: 759 1262 1460 2144 2314 759
Size: 503 198 684 170 3741
Fragments arranged by size:
3741 684 503 198 170
BamHI G'GATC_C
Cuts at: 1512 2319 1512
Size: 807 4489
BanI G'GyrC_C
Cuts at: 582 591 1228 1317 1456 1971 2310 2724 2972
Size: 9 637 89 139 515 339 414 248
Cuts at: 2972 3007 582
Size: 35 2871
Fragments arranged by size:
2871 637 515 414 339 248 139 89
35 9
BanII G_rGCy'C
Cuts at: 267 424 534 889 927 2308 3338 267
Size: 157 110 355 38 1381 1030 2225
Fragments arranged by size:
2225 1381 1030 355 157 110 38
BbeI G_GCGC'C
Cuts at: 595 1460 2728 2976 595
Size: 865 1268 248 2915
Fragments arranged by size:
2915 1268 865 248
BbsI GAAGACnn'nnnn_
Cuts at: 2039 2146 2039
Size: 107 5189
BbvCI CC'TCA_GC
Cuts at: 1759 1759
Size: 5296
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3955 5296 3192
Size: 763 1341 3192
Fragments arranged by size:
3192 1341 763
BclI T'GATC_A
Cuts at: 608 2814 608
Size: 2206 3090
BglI GCCn_nnn'nGGC
Cuts at: 1698 1698
Size: 5296
BglII A'GATC_T
Cuts at: 2809 4848 2809
Size: 2039 3257
BlpI GC'TnA_GC
Cuts at: 673 888 2057 2406 673
Size: 215 1169 349 3563
Fragments arranged by size:
3563 1169 349 215
Bme1580I G_kGCm'C
Cuts at: 267 424 587 1233 1322 2919 3012 4070 267
Size: 157 163 646 89 1597 93 1058 1493
Fragments arranged by size:
1597 1493 1058 646 163 157 93 89
BmgBI CAC'GTC
Cuts at: 569 1392 569
Size: 823 4473
BmrI ACTGGGnnnn_n'
Cuts at: 1996 2506 2587 2922 5068 1996
Size: 510 81 335 2146 2224
Fragments arranged by size:
2224 2146 510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1291 3661 1291
Size: 2370 2926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 582 743 1264 1591 1605 2197 436
Size: 146 161 521 327 14 592 3535
Fragments arranged by size:
3535 592 521 327 161 146 14
Bpu10I CC'TnA_GC
Cuts at: 1759 1759
Size: 5296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2470 3349 3843 4141 2470
Size: 879 494 298 3625
Fragments arranged by size:
3625 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1355 1373 5000 1355
Size: 18 3627 1651
Fragments arranged by size:
3627 1651 18
BsaAI yAC'GTr
Cuts at: 2286 2647 2286
Size: 361 4935
BsaBI GATnn'nnATC
Cuts at: 6 1918 2808 4847 6
Size: 1912 890 2039 455
Fragments arranged by size:
2039 1912 890 455
BsaHI Gr'CG_yC
Cuts at: 592 838 1457 2725 2973 3677 5173 592
Size: 246 619 1268 248 704 1496 715
Fragments arranged by size:
1496 1268 715 704 619 248 246
BsaWI w'CCGG_w
Cuts at: 387 703 1338 2483 3004 3684 3958 4105 4516
Size: 316 635 1145 521 680 274 147 411
Cuts at: 4516 387
Size: 1167
Fragments arranged by size:
1167 1145 680 635 521 411 316 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1603 1633 1603
Size: 30 5266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 826 1192 1208 1211 1267 1280 1298 1800 1912
Size: 366 16 3 56 13 18 502 112
Cuts at: 1912 826
Size: 4210
Fragments arranged by size:
4210 502 366 112 56 18 16 13
3
BseYI C'CCAG_C
Cuts at: 234 677 897 1321 2719 3477 4056 4552 234
Size: 443 220 424 1398 758 579 496 978
Fragments arranged by size:
1398 978 758 579 496 443 424 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 850 1319 1666 496
Size: 354 469 347 4126
Fragments arranged by size:
4126 469 354 347
BsiEI CG_ry'CG
Cuts at: 104 2836 2882 4092 104
Size: 2732 46 1210 1308
Fragments arranged by size:
2732 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 568 864 889 906 927 2308 3086 3276 4070
Size: 296 25 17 21 1381 778 190 794
Cuts at: 4070 568
Size: 1794
Fragments arranged by size:
1794 1381 794 778 296 190 25 21
17
BsmI GAATG_Cn'
Cuts at: 1305 2132 1305
Size: 827 4469
BsmBI CGTCTCn'nnnn_
Cuts at: 1382 1382
Size: 5296
Bsp1286I G_dGCh'C
Cuts at: 267 424 534 568 587 864 889 906 927
Size: 157 110 34 19 277 25 17 21
Cuts at: 927 1233 1322 2308 2919 3012 3086 3276 3338
Size: 306 89 986 611 93 74 190 62
Cuts at: 3338 4070 267
Size: 732 1493
Fragments arranged by size:
1493 986 732 611 306 277 190 157
110 93 89 74 62 34 25 21
19 17
BspEI T'CCGG_A
Cuts at: 387 703 4516 387
Size: 316 3813 1167
Fragments arranged by size:
3813 1167 316
BspHI T'CATG_A
Cuts at: 4472 4472
Size: 5296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1341 2344 2558 2860 3241 1341
Size: 1003 214 302 381 3396
Fragments arranged by size:
3396 1003 381 302 214
BsrBI CCG'CTC
Cuts at: 50 669 2157 3586 3640 5126 5291 50
Size: 619 1488 1429 54 1486 165 55
Fragments arranged by size:
1488 1486 1429 619 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1637 3206 1631
Size: 6 1569 3721
Fragments arranged by size:
3721 1569 6
BsrFI r'CCGG_y
Cuts at: 3292 3473 3684 3292
Size: 181 211 4904
Fragments arranged by size:
4904 211 181
BssSI C'ACGA_G
Cuts at: 1784 3565 3925 1784
Size: 1781 360 3155
Fragments arranged by size:
3155 1781 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2671 2671
Size: 5296
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1666 1666
Size: 5296
BstXI CCAn_nnnn'nTGG
Cuts at: 1050 1050
Size: 5296
BstYI r'GATC_y
Cuts at: 1 961 1512 1805 2319 2786 2809 3144 3390
Size: 960 551 293 514 467 23 335 246
Cuts at: 3390 4404 4490 4502 4848 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 960 551 514 467 449 346 335
293 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1108 1108
Size: 5296
BtgI C'CryG_G
Cuts at: 1233 1348 1592 1662 1233
Size: 115 244 70 4867
Fragments arranged by size:
4867 244 115 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3244 3425 3588 3244
Size: 181 163 4952
Fragments arranged by size:
4952 181 163
BtsI GCAGTG_nn'
Cuts at: 513 2537 513
Size: 2024 3272
DraI TTT'AAA
Cuts at: 2293 2293
Size: 5296
DraIII CAC_nnn'GTG
Cuts at: 1036 1526 2205 1036
Size: 490 679 4127
Fragments arranged by size:
4127 679 490
DrdI GACnn_nn'nnGTC
Cuts at: 1681 2043 3000 3860 1681
Size: 362 957 860 3117
Fragments arranged by size:
3117 957 860 362
EaeI y'GGCC_r
Cuts at: 1545 1589 2879 3053 3444 3471 4818 1545
Size: 44 1290 174 391 27 1347 2023
Fragments arranged by size:
2023 1347 1290 391 174 44 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5296
EarI CTCTTCn'nnn_
Cuts at: 650 1076 1841 1969 2105 3317 3527 650
Size: 426 765 128 136 1212 210 2419
Fragments arranged by size:
2419 1212 765 426 210 136 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 897 1549 1909 3502 3814 3960 5106 897
Size: 652 360 1593 312 146 1146 1087
Fragments arranged by size:
1593 1146 1087 652 360 312 146
EcoICRI GAG'CTC
Cuts at: 887 925 2306 887
Size: 38 1381 3877
Fragments arranged by size:
3877 1381 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 336 436 478 582 644 743 1264 1591
Size: 182 100 42 104 62 99 521 327
Cuts at: 1591 1605 1689 1865 2041 2197 3119 3551 4300
Size: 14 84 176 176 156 922 432 749
Cuts at: 4300 4568 154
Size: 268 882
Fragments arranged by size:
922 882 749 521 432 327 268 182
176 176 156 104 100 99 84 62
42 14
EcoNI CCTnn'n_nnAGG
Cuts at: 376 1171 1245 1894 376
Size: 795 74 649 3778
Fragments arranged by size:
3778 795 649 74
EcoO109I rG'GnC_Cy
Cuts at: 263 420 421 462 970 1164 1438 1503 1887
Size: 157 1 41 508 194 274 65 384
Cuts at: 1887 2262 2433 263
Size: 375 171 3126
Fragments arranged by size:
3126 508 384 375 274 194 171 157
65 41 1
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3313 3313
Size: 5296
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5296
HaeII r_GCGC'y
Cuts at: 595 1460 1704 2728 2976 4000 4979 595
Size: 865 244 1024 248 1024 979 912
Fragments arranged by size:
1024 1024 979 912 865 248 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 3136 3166 3168 3198 946
Size: 32 2158 30 2 30 3044
Fragments arranged by size:
3044 2158 32 30 30 2
HincII GTy'rAC
Cuts at: 2333 2333
Size: 5296
KasI G'GCGC_C
Cuts at: 591 1456 2724 2972 591
Size: 865 1268 248 2915
Fragments arranged by size:
2915 1268 865 248
KpnI G_GTAC'C
Cuts at: 1975 2314 1975
Size: 339 4957
MluI A'CGCG_T
Cuts at: 4729 4729
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4151 3967
Size: 184 5112
MscI TGG'CCA
Cuts at: 1547 1591 3055 1547
Size: 44 1464 3788
Fragments arranged by size:
3788 1464 44
MslI CAynn'nnrTG
Cuts at: 1642 3283 3410 1642
Size: 1641 127 3528
Fragments arranged by size:
3528 1641 127
MspA1I CmG'CkG
Cuts at: 414 1284 2172 2223 2405 2719 3079 4094 4339
Size: 870 888 51 182 314 360 1015 245
Cuts at: 4339 414
Size: 1371
Fragments arranged by size:
1371 1015 888 870 360 314 245 182
51
NaeI GCC'GGC
Cuts at: 3475 3475
Size: 5296
NarI GG'CG_CC
Cuts at: 592 1457 2725 2973 592
Size: 865 1268 248 2915
Fragments arranged by size:
2915 1268 865 248
NcoI C'CATG_G
Cuts at: 1233 1348 1592 1233
Size: 115 244 4937
Fragments arranged by size:
4937 244 115
NgoMIV G'CCGG_C
Cuts at: 3473 3473
Size: 5296
NruI TCG'CGA
Cuts at: 3657 3657
Size: 5296
NspI r_CATG'y
Cuts at: 2347 2847 3756 2347
Size: 500 909 3887
Fragments arranged by size:
3887 909 500
PasI CC'CwG_GG
Cuts at: 534 587 1043 1167 534
Size: 53 456 124 4663
Fragments arranged by size:
4663 456 124 53
PciI A'CATG_T
Cuts at: 3752 3752
Size: 5296
PfoI T'CCnGG_A
Cuts at: 806 1883 806
Size: 1077 4219
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4435 4467 4435
Size: 32 5264
PpuMI rG'GwC_Cy
Cuts at: 970 1887 970
Size: 917 4379
PshAI GACnn'nnGTC
Cuts at: 1664 1664
Size: 5296
PspOMI G'GGCC_C
Cuts at: 263 420 263
Size: 157 5139
PstI C_TGCA'G
Cuts at: 161 413 472 485 755 1511 2222 2341 3026
Size: 252 59 13 270 756 711 119 685
Cuts at: 3026 161
Size: 2431
Fragments arranged by size:
2431 756 711 685 270 252 119 59
13
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5296
PvuII CAG'CTG
Cuts at: 414 2172 2719 3079 414
Size: 1758 547 360 2631
Fragments arranged by size:
2631 1758 547 360
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5296
SacI G_AGCT'C
Cuts at: 889 927 2308 889
Size: 38 1381 3877
Fragments arranged by size:
3877 1381 38
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5296
SanDI GG'GwC_CC
Cuts at: 970 970
Size: 5296
SapI GCTCTTCn'nnn_
Cuts at: 650 3317 3527 650
Size: 2667 210 2419
Fragments arranged by size:
2667 2419 210
SbfI CC_TGCA'GG
Cuts at: 1511 2341 1511
Size: 830 4466
ScaI AGT'ACT
Cuts at: 1651 4813 1651
Size: 3162 2134
SfcI C'TryA_G
Cuts at: 33 157 409 468 481 751 1507 2218 2337
Size: 124 252 59 13 270 756 711 119
Cuts at: 2337 3022 4017 4208 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 756 711 685 270 252 191
124 119 59 13
SfoI GGC'GCC
Cuts at: 593 1458 2726 2974 593
Size: 865 1268 248 2915
Fragments arranged by size:
2915 1268 865 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5296
SmaI CCC'GGG
Cuts at: 761 2146 2316 761
Size: 1385 170 3741
Fragments arranged by size:
3741 1385 170
SmlI C'TyrA_G
Cuts at: 2449 3364 3858 4120 2449
Size: 915 494 262 3625
Fragments arranged by size:
3625 915 494 262
SnaBI TAC'GTA
Cuts at: 2286 2286
Size: 5296
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5296
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4796
StyI C'CwwG_G
Cuts at: 425 446 912 1233 1348 1592 2428 2782 425
Size: 21 466 321 115 244 836 354 2939
Fragments arranged by size:
2939 836 466 354 321 244 115 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 889 1114 2877 3010 3469 3680 889
Size: 225 1763 133 459 211 2505
Fragments arranged by size:
2505 1763 459 225 211 133
TatI w'GTAC_w
Cuts at: 718 1649 4811 718
Size: 931 3162 1203
Fragments arranged by size:
3162 1203 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1020 1045 1052 1072 1077 1104 1603 1635 4813
Size: 25 7 20 5 27 499 32 3178
Cuts at: 4813 4845 1020
Size: 32 1471
Fragments arranged by size:
3178 1471 499 32 32 27 25 20
7 5
Tth111I GACn'n_nGTC
Cuts at: 3091 3091
Size: 5296
XbaI T'CTAG_A
Cuts at: 2325 2325
Size: 5296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2789 2789
Size: 5296
XmaI C'CCGG_G
Cuts at: 759 2144 2314 759
Size: 1385 170 3741
Fragments arranged by size:
3741 1385 170
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PflMI PmlI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI