Restriction map for
pF1KSDA0332
(Circular) (Six-base) MAPSORT of: pF1KSDA0332.seq Check: 4189 from: 1 to: 6226
pF1KSDA0332 6226 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2949 3262 2949
Size: 313 5913
Acc65I G'GTAC_C
Cuts at: 3240 3240
Size: 6226
AclI AA'CG_TT
Cuts at: 6066 6066
Size: 6226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 1847 1856 1925 2070 2378 2579 4049 4481
Size: 1659 9 69 145 308 201 1470 432
Cuts at: 4481 5230 5498 188
Size: 749 268 916
Fragments arranged by size:
1659 1470 916 749 432 308 268 201
145 69 9
AflII C'TTAA_G
Cuts at: 1726 1726
Size: 6226
AflIII A'CryG_T
Cuts at: 3576 4682 5659 3576
Size: 1106 977 4143
Fragments arranged by size:
4143 1106 977
AgeI A'CCGG_T
Cuts at: 4614 4614
Size: 6226
AlwNI CAG_nnn'CTG
Cuts at: 466 838 3529 5098 466
Size: 372 2691 1569 1594
Fragments arranged by size:
2691 1594 1569 372
ApaLI G'TGCA_C
Cuts at: 2240 4996 2240
Size: 2756 3470
ApoI r'AATT_y
Cuts at: 764 1003 1134 1401 1975 2264 3228 5579 5835
Size: 239 131 267 574 289 964 2351 256
Cuts at: 5835 764
Size: 1155
Fragments arranged by size:
2351 1155 964 574 289 267 256 239
131
AseI AT'TA_AT
Cuts at: 20 968 20
Size: 948 5278
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6226
AvaI C'yCGr_G
Cuts at: 3244 3244
Size: 6226
BamHI G'GATC_C
Cuts at: 3249 3249
Size: 6226
BanI G'GyrC_C
Cuts at: 2212 3240 3654 3902 3937 2212
Size: 1028 414 248 35 4501
Fragments arranged by size:
4501 1028 414 248 35
BanII G_rGCy'C
Cuts at: 274 2672 3019 3238 4268 274
Size: 2398 347 219 1030 2232
Fragments arranged by size:
2398 2232 1030 347 219
BbeI G_GCGC'C
Cuts at: 3658 3906 3658
Size: 248 5978
BbsI GAAGACnn'nnnn_
Cuts at: 638 1360 2366 638
Size: 722 1006 4498
Fragments arranged by size:
4498 1006 722
BciVI GTATCCnnnnn_n'
Cuts at: 4122 4885 6226 4122
Size: 763 1341 4122
Fragments arranged by size:
4122 1341 763
BclI T'GATC_A
Cuts at: 2129 3744 2129
Size: 1615 4611
BfrBI ATG'CAT
Cuts at: 223 223
Size: 6226
BglII A'GATC_T
Cuts at: 3001 3127 3169 3739 5778 3001
Size: 126 42 570 2039 3449
Fragments arranged by size:
3449 2039 570 126 42
BlpI GC'TnA_GC
Cuts at: 401 3336 401
Size: 2935 3291
Bme1580I G_kGCm'C
Cuts at: 306 2217 2244 3849 3942 5000 306
Size: 1911 27 1605 93 1058 1532
Fragments arranged by size:
1911 1605 1532 1058 93 27
BmrI ACTGGGnnnn_n'
Cuts at: 3156 3436 3517 3852 5998 3156
Size: 280 81 335 2146 3384
Fragments arranged by size:
3384 2146 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4591 4591
Size: 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 3400 4279 4773 5071 327
Size: 3073 879 494 298 1482
Fragments arranged by size:
3073 1482 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3188 5930 3188
Size: 2742 3484
BsaAI yAC'GTr
Cuts at: 1444 3216 3577 1444
Size: 1772 361 4093
Fragments arranged by size:
4093 1772 361
BsaBI GATnn'nnATC
Cuts at: 6 2327 3081 3738 5777 6
Size: 2321 754 657 2039 455
Fragments arranged by size:
2321 2039 754 657 455
BsaHI Gr'CG_yC
Cuts at: 3655 3903 4607 6103 3655
Size: 248 704 1496 3778
Fragments arranged by size:
3778 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1261 3413 3934 4614 4888 5035 5446 1261
Size: 2152 521 680 274 147 411 2041
Fragments arranged by size:
2152 2041 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 317 493 815 317
Size: 176 322 5728
Fragments arranged by size:
5728 322 176
BseYI C'CCAG_C
Cuts at: 2923 3649 4407 4986 5482 2923
Size: 726 758 579 496 3667
Fragments arranged by size:
3667 758 726 579 496
BsiEI CG_ry'CG
Cuts at: 104 3766 3812 5022 104
Size: 3662 46 1210 1308
Fragments arranged by size:
3662 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1725 2244 2672 3019 3238 4016 4206 5000 1725
Size: 519 428 347 219 778 190 794 2951
Fragments arranged by size:
2951 794 778 519 428 347 219 190
BsmI GAATG_Cn'
Cuts at: 373 373
Size: 6226
Bsp1286I G_dGCh'C
Cuts at: 274 306 1725 2217 2244 2672 3019 3238 3849
Size: 32 1419 492 27 428 347 219 611
Cuts at: 3849 3942 4016 4206 4268 5000 274
Size: 93 74 190 62 732 1500
Fragments arranged by size:
1500 1419 732 611 492 428 347 219
190 93 74 62 32 27
BspEI T'CCGG_A
Cuts at: 1261 5446 1261
Size: 4185 2041
BspHI T'CATG_A
Cuts at: 5402 5402
Size: 6226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3274 3488 3790 4171 3274
Size: 214 302 381 5329
Fragments arranged by size:
5329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1293 4516 4570 6056 6221 50
Size: 1243 3223 54 1486 165 55
Fragments arranged by size:
3223 1486 1243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 4136 1808
Size: 2328 3898
BsrFI r'CCGG_y
Cuts at: 4222 4403 4614 4222
Size: 181 211 5834
Fragments arranged by size:
5834 211 181
BsrGI T'GTAC_A
Cuts at: 1931 1931
Size: 6226
BssSI C'ACGA_G
Cuts at: 4495 4855 4495
Size: 360 5866
BstAPI GCAn_nnn'nTGC
Cuts at: 3601 3601
Size: 6226
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6226
BstXI CCAn_nnnn'nTGG
Cuts at: 1006 1589 2405 1006
Size: 583 816 4827
Fragments arranged by size:
4827 816 583
BstYI r'GATC_y
Cuts at: 1 148 199 933 1624 3001 3127 3157 3169
Size: 147 51 734 691 1377 126 30 12
Cuts at: 3169 3249 3716 3739 4074 4320 5334 5420 5432
Size: 80 467 23 335 246 1014 86 12
Cuts at: 5432 5778 1
Size: 346 449
Fragments arranged by size:
1377 1014 734 691 467 449 346 335
246 147 126 86 80 51 30 23
12 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 6226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4174 4355 4518 4174
Size: 181 163 5882
Fragments arranged by size:
5882 181 163
BtsI GCAGTG_nn'
Cuts at: 2949 3467 2949
Size: 518 5708
ClaI AT'CG_AT
Cuts at: 2288 2288
Size: 6226
DraI TTT'AAA
Cuts at: 1130 2388 3223 1130
Size: 1258 835 4133
Fragments arranged by size:
4133 1258 835
DrdI GACnn_nn'nnGTC
Cuts at: 3930 4790 3930
Size: 860 5366
EaeI y'GGCC_r
Cuts at: 3809 3983 4374 4401 5748 3809
Size: 174 391 27 1347 4287
Fragments arranged by size:
4287 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3809 3809
Size: 6226
EarI CTCTTCn'nnn_
Cuts at: 356 674 748 1104 1670 1944 2222 2896 4247
Size: 318 74 356 566 274 278 674 1351
Cuts at: 4247 4457 356
Size: 210 2125
Fragments arranged by size:
2125 1351 674 566 356 318 278 274
210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 161 1211 1608 4432 4744 4890 6036 145
Size: 16 1050 397 2824 312 146 1146 335
Fragments arranged by size:
2824 1146 1050 397 335 312 146 16
EcoICRI GAG'CTC
Cuts at: 2670 3017 3236 2670
Size: 347 219 5660
Fragments arranged by size:
5660 347 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 1847 1856 1925 2070 2378 2579 4049 4481
Size: 1659 9 69 145 308 201 1470 432
Cuts at: 4481 5230 5498 188
Size: 749 268 916
Fragments arranged by size:
1659 1470 916 749 432 308 268 201
145 69 9
EcoO109I rG'GnC_Cy
Cuts at: 3363 3363
Size: 6226
EcoRI G'AATT_C
Cuts at: 2264 3228 2264
Size: 964 5262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4243 4243
Size: 6226
FspI TGC'GCA
Cuts at: 1280 4005 1280
Size: 2725 3501
HaeII r_GCGC'y
Cuts at: 3658 3906 4930 5909 3658
Size: 248 1024 979 3975
Fragments arranged by size:
3975 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 578 610 1852 1884 4066 4096 4098 4128 578
Size: 32 1242 32 2182 30 2 30 2676
Fragments arranged by size:
2676 2182 1242 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3263 3263
Size: 6226
HindIII A'AGCT_T
Cuts at: 1552 1691 1552
Size: 139 6087
KasI G'GCGC_C
Cuts at: 3654 3902 3654
Size: 248 5978
KpnI G_GTAC'C
Cuts at: 3244 3244
Size: 6226
MluI A'CGCG_T
Cuts at: 5659 5659
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 235 1606 2908 4897 5081 235
Size: 1371 1302 1989 184 1380
Fragments arranged by size:
1989 1380 1371 1302 184
MscI TGG'CCA
Cuts at: 3985 3985
Size: 6226
MslI CAynn'nnrTG
Cuts at: 218 2403 4213 4340 218
Size: 2185 1810 127 2104
Fragments arranged by size:
2185 2104 1810 127
MspA1I CmG'CkG
Cuts at: 463 3335 3649 4009 5024 5269 463
Size: 2872 314 360 1015 245 1420
Fragments arranged by size:
2872 1420 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4405 4405
Size: 6226
NarI GG'CG_CC
Cuts at: 3655 3903 3655
Size: 248 5978
NcoI C'CATG_G
Cuts at: 125 125
Size: 6226
NdeI CA'TA_TG
Cuts at: 215 215
Size: 6226
NgoMIV G'CCGG_C
Cuts at: 4403 4403
Size: 6226
NruI TCG'CGA
Cuts at: 4587 4587
Size: 6226
NsiI A_TGCA'T
Cuts at: 225 225
Size: 6226
NspI r_CATG'y
Cuts at: 3277 3777 4686 3277
Size: 500 909 4817
Fragments arranged by size:
4817 909 500
PciI A'CATG_T
Cuts at: 4682 4682
Size: 6226
PmeI GTTT'AAAC
Cuts at: 3223 3223
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2628 5365 5397 2596
Size: 32 2737 32 3425
Fragments arranged by size:
3425 2737 32 32
PstI C_TGCA'G
Cuts at: 1571 3271 3956 1571
Size: 1700 685 3841
Fragments arranged by size:
3841 1700 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6226
PvuII CAG'CTG
Cuts at: 463 3649 4009 463
Size: 3186 360 2680
Fragments arranged by size:
3186 2680 360
RsrII CG'GwC_CG
Cuts at: 4419 4419
Size: 6226
SacI G_AGCT'C
Cuts at: 2672 3019 3238 2672
Size: 347 219 5660
Fragments arranged by size:
5660 347 219
SalI G'TCGA_C
Cuts at: 3261 3261
Size: 6226
SapI GCTCTTCn'nnn_
Cuts at: 1670 2222 4247 4457 1670
Size: 552 2025 210 3439
Fragments arranged by size:
3439 2025 552 210
SbfI CC_TGCA'GG
Cuts at: 3271 3271
Size: 6226
ScaI AGT'ACT
Cuts at: 2623 5743 2623
Size: 3120 3106
SfcI C'TryA_G
Cuts at: 33 1542 1567 2757 3267 3952 4947 5138 33
Size: 1509 25 1190 510 685 995 191 1121
Fragments arranged by size:
1509 1190 1121 995 685 510 191 25
SfoI GGC'GCC
Cuts at: 3656 3904 3656
Size: 248 5978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6226
SmaI CCC'GGG
Cuts at: 3246 3246
Size: 6226
SmlI C'TyrA_G
Cuts at: 342 1726 3379 4294 4788 5050 342
Size: 1384 1653 915 494 262 1518
Fragments arranged by size:
1653 1518 1384 915 494 262
SnaBI TAC'GTA
Cuts at: 3216 3216
Size: 6226
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6226
SphI G_CATG'C
Cuts at: 3277 3777 3277
Size: 500 5726
SspI AAT'ATT
Cuts at: 2145 2166 2145
Size: 21 6205
StyI C'CwwG_G
Cuts at: 125 957 1777 3358 3712 125
Size: 832 820 1581 354 2639
Fragments arranged by size:
2639 1581 832 820 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3807 3940 4399 4610 3807
Size: 133 459 211 5423
Fragments arranged by size:
5423 459 211 133
TatI w'GTAC_w
Cuts at: 394 1931 2573 2621 2878 2914 5741 394
Size: 1537 642 48 257 36 2827 879
Fragments arranged by size:
2827 1537 879 642 257 48 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 285 317 2902 2934 5743 5775 285
Size: 32 2585 32 2809 32 736
Fragments arranged by size:
2809 2585 736 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1359 2604 4021 1359
Size: 1245 1417 3564
Fragments arranged by size:
3564 1417 1245
XbaI T'CTAG_A
Cuts at: 3124 3255 3124
Size: 131 6095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1377 1911 3719 1377
Size: 534 1808 3884
Fragments arranged by size:
3884 1808 534
XmaI C'CCGG_G
Cuts at: 3244 3244
Size: 6226
XmnI GAAnn'nnTTC
Cuts at: 1604 1604
Size: 6226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
ZraI