Restriction map for
pF1KSDA0334
(Circular) (Six-base) MAPSORT of: pF1KSDA0334.seq Check: 6213 from: 1 to: 5677
pF1KSDA0334 5677 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2713 2713
Size: 5677
Acc65I G'GTAC_C
Cuts at: 2298 2691 2298
Size: 393 5284
AclI AA'CG_TT
Cuts at: 5517 5517
Size: 5677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1162 1774 2414 3500 3932 4681 4949 791
Size: 371 612 640 1086 432 749 268 1519
Fragments arranged by size:
1519 1086 749 640 612 432 371 268
AflIII A'CryG_T
Cuts at: 3027 4133 5110 3027
Size: 1106 977 3594
Fragments arranged by size:
3594 1106 977
AgeI A'CCGG_T
Cuts at: 4065 4065
Size: 5677
AleI CACnn'nnGTG
Cuts at: 2119 2119
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 1324 1812 2980 4549 1324
Size: 488 1168 1569 2452
Fragments arranged by size:
2452 1569 1168 488
ApaLI G'TGCA_C
Cuts at: 924 4447 924
Size: 3523 2154
ApoI r'AATT_y
Cuts at: 410 700 766 777 1083 1212 1581 1635 1775
Size: 290 66 11 306 129 369 54 140
Cuts at: 1775 1848 1878 2679 5030 5286 410
Size: 73 30 801 2351 256 801
Fragments arranged by size:
2351 801 801 369 306 290 256 140
129 73 66 54 30 11
AseI AT'TA_AT
Cuts at: 20 463 20
Size: 443 5234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5677
AvaI C'yCGr_G
Cuts at: 2695 2695
Size: 5677
BamHI G'GATC_C
Cuts at: 298 2700 298
Size: 2402 3275
BanI G'GyrC_C
Cuts at: 1002 2298 2691 3105 3353 3388 1002
Size: 1296 393 414 248 35 3291
Fragments arranged by size:
3291 1296 414 393 248 35
BanII G_rGCy'C
Cuts at: 160 2410 2689 3719 160
Size: 2250 279 1030 2118
Fragments arranged by size:
2250 2118 1030 279
BbeI G_GCGC'C
Cuts at: 3109 3357 3109
Size: 248 5429
BciVI GTATCCnnnnn_n'
Cuts at: 810 1491 3573 4336 5677 810
Size: 681 2082 763 1341 810
Fragments arranged by size:
2082 1341 810 763 681
BclI T'GATC_A
Cuts at: 1398 3195 1398
Size: 1797 3880
BglII A'GATC_T
Cuts at: 3190 5229 3190
Size: 2039 3638
BlpI GC'TnA_GC
Cuts at: 2453 2787 2453
Size: 334 5343
Bme1580I G_kGCm'C
Cuts at: 928 1005 2054 3300 3393 4451 928
Size: 77 1049 1246 93 1058 2154
Fragments arranged by size:
2154 1246 1058 1049 93 77
BmrI ACTGGGnnnn_n'
Cuts at: 304 1581 1656 2069 2887 2968 3303 5449 304
Size: 1277 75 413 818 81 335 2146 532
Fragments arranged by size:
2146 1277 818 532 413 335 81 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4042 4042
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 1610 2222 2851 3730 4224 4522 1360
Size: 250 612 629 879 494 298 2515
Fragments arranged by size:
2515 879 629 612 494 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 5381 5381
Size: 5677
BsaAI yAC'GTr
Cuts at: 2667 3028 2667
Size: 361 5316
BsaBI GATnn'nnATC
Cuts at: 6 1061 3189 5228 6
Size: 1055 2128 2039 455
Fragments arranged by size:
2128 2039 1055 455
BsaHI Gr'CG_yC
Cuts at: 3106 3354 4058 5554 3106
Size: 248 704 1496 3229
Fragments arranged by size:
3229 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2864 3385 4065 4339 4486 4897 2864
Size: 521 680 274 147 411 3644
Fragments arranged by size:
3644 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1491 1521 1491
Size: 30 5647
BseYI C'CCAG_C
Cuts at: 2462 3100 3858 4437 4933 2462
Size: 638 758 579 496 3206
Fragments arranged by size:
3206 758 638 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 374
Size: 5677
BsiEI CG_ry'CG
Cuts at: 104 1457 3217 3263 4473 104
Size: 1353 1760 46 1210 1308
Fragments arranged by size:
1760 1353 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 160 928 1106 1507 2092 2410 2560 2689 3467
Size: 768 178 401 585 318 150 129 778
Cuts at: 3467 3657 4451 160
Size: 190 794 1386
Fragments arranged by size:
1386 794 778 768 585 401 318 190
178 150 129
BsmBI CGTCTCn'nnnn_
Cuts at: 1464 1464
Size: 5677
Bsp1286I G_dGCh'C
Cuts at: 160 928 1005 1106 1507 2054 2092 2410 2560
Size: 768 77 101 401 547 38 318 150
Cuts at: 2560 2689 3300 3393 3467 3657 3719 4451 160
Size: 129 611 93 74 190 62 732 1386
Fragments arranged by size:
1386 768 732 611 547 401 318 190
150 129 101 93 77 74 62 38
BspEI T'CCGG_A
Cuts at: 4897 4897
Size: 5677
BspHI T'CATG_A
Cuts at: 500 1098 1533 4853 500
Size: 598 435 3320 1324
Fragments arranged by size:
3320 1324 598 435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2725 2939 3241 3622 2725
Size: 214 302 381 4780
Fragments arranged by size:
4780 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3967 4021 5507 5672 50
Size: 3917 54 1486 165 55
Fragments arranged by size:
3917 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3587 3587
Size: 5677
BsrFI r'CCGG_y
Cuts at: 2615 3673 3854 4065 2615
Size: 1058 181 211 4227
Fragments arranged by size:
4227 1058 211 181
BssSI C'ACGA_G
Cuts at: 3946 4306 3946
Size: 360 5317
BstAPI GCAn_nnn'nTGC
Cuts at: 1110 3052 1110
Size: 1942 3735
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5677
BstXI CCAn_nnnn'nTGG
Cuts at: 1342 1594 1342
Size: 252 5425
BstYI r'GATC_y
Cuts at: 1 298 1487 2700 3167 3190 3525 3771 4785
Size: 297 1189 1213 467 23 335 246 1014
Cuts at: 4785 4871 4883 5229 1
Size: 86 12 346 449
Fragments arranged by size:
1213 1189 1014 467 449 346 335 297
246 86 23 12
BtgI C'CryG_G
Cuts at: 1800 2502 1800
Size: 702 4975
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3625 3806 3969 3625
Size: 181 163 5333
Fragments arranged by size:
5333 181 163
BtsI GCAGTG_nn'
Cuts at: 386 2918 386
Size: 2532 3145
DraI TTT'AAA
Cuts at: 680 791 2674 680
Size: 111 1883 3683
Fragments arranged by size:
3683 1883 111
DrdI GACnn_nn'nnGTC
Cuts at: 3381 4241 3381
Size: 860 4817
EaeI y'GGCC_r
Cuts at: 1454 3260 3434 3825 3852 5199 1454
Size: 1806 174 391 27 1347 1932
Fragments arranged by size:
1932 1806 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1454 3260 1454
Size: 1806 3871
EarI CTCTTCn'nnn_
Cuts at: 443 810 941 1295 1313 1428 3698 3908 443
Size: 367 131 354 18 115 2270 210 2212
Fragments arranged by size:
2270 2212 367 354 210 131 115 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3883 4195 4341 5487 3883
Size: 312 146 1146 4073
Fragments arranged by size:
4073 1146 312 146
EcoICRI GAG'CTC
Cuts at: 158 2408 2687 158
Size: 2250 279 3148
Fragments arranged by size:
3148 2250 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1162 1774 2414 3500 3932 4681 4949 791
Size: 371 612 640 1086 432 749 268 1519
Fragments arranged by size:
1519 1086 749 640 612 432 371 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1153 1153
Size: 5677
EcoO109I rG'GnC_Cy
Cuts at: 2814 2814
Size: 5677
EcoRI G'AATT_C
Cuts at: 700 1212 2679 700
Size: 512 1467 3698
Fragments arranged by size:
3698 1467 512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1324 1804 3694 1324
Size: 480 1890 3307
Fragments arranged by size:
3307 1890 480
FspI TGC'GCA
Cuts at: 3456 3456
Size: 5677
HaeII r_GCGC'y
Cuts at: 3109 3357 4381 5360 3109
Size: 248 1024 979 3426
Fragments arranged by size:
3426 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 1330 1362 1456 1488 2322 2354 3517
Size: 32 358 32 94 32 834 32 1163
Cuts at: 3517 3547 3549 3579 940
Size: 30 2 30 3038
Fragments arranged by size:
3038 1163 834 358 94 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 168 2032 2245 2714 168
Size: 1864 213 469 3131
Fragments arranged by size:
3131 1864 469 213
KasI G'GCGC_C
Cuts at: 3105 3353 3105
Size: 248 5429
KpnI G_GTAC'C
Cuts at: 2302 2695 2302
Size: 393 5284
MfeI C'AATT_G
Cuts at: 1711 1711
Size: 5677
MluI A'CGCG_T
Cuts at: 5110 5110
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4348 4532 4348
Size: 184 5493
MscI TGG'CCA
Cuts at: 3436 3436
Size: 5677
MslI CAynn'nnrTG
Cuts at: 1070 2119 3664 3791 1070
Size: 1049 1545 127 2956
Fragments arranged by size:
2956 1545 1049 127
MspA1I CmG'CkG
Cuts at: 1321 2457 2786 3100 3460 4475 4720 1321
Size: 1136 329 314 360 1015 245 2278
Fragments arranged by size:
2278 1136 1015 360 329 314 245
NaeI GCC'GGC
Cuts at: 2617 3856 2617
Size: 1239 4438
NarI GG'CG_CC
Cuts at: 3106 3354 3106
Size: 248 5429
NcoI C'CATG_G
Cuts at: 1800 2502 1800
Size: 702 4975
NgoMIV G'CCGG_C
Cuts at: 2615 3854 2615
Size: 1239 4438
NruI TCG'CGA
Cuts at: 4038 4038
Size: 5677
NspI r_CATG'y
Cuts at: 717 1659 2728 3228 4137 717
Size: 942 1069 500 909 2257
Fragments arranged by size:
2257 1069 942 909 500
PciI A'CATG_T
Cuts at: 4133 4133
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2674 2674
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4816 4848 4816
Size: 32 5645
PsiI TTA'TAA
Cuts at: 626 626
Size: 5677
PstI C_TGCA'G
Cuts at: 350 1817 2139 2199 2722 3407 350
Size: 1467 322 60 523 685 2620
Fragments arranged by size:
2620 1467 685 523 322 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5677
PvuII CAG'CTG
Cuts at: 1321 2457 3100 3460 1321
Size: 1136 643 360 3538
Fragments arranged by size:
3538 1136 643 360
RsrII CG'GwC_CG
Cuts at: 3870 3870
Size: 5677
SacI G_AGCT'C
Cuts at: 160 2410 2689 160
Size: 2250 279 3148
Fragments arranged by size:
3148 2250 279
SalI G'TCGA_C
Cuts at: 2712 2712
Size: 5677
SapI GCTCTTCn'nnn_
Cuts at: 3698 3908 3698
Size: 210 5467
SbfI CC_TGCA'GG
Cuts at: 2722 2722
Size: 5677
ScaI AGT'ACT
Cuts at: 2307 5194 2307
Size: 2887 2790
SfcI C'TryA_G
Cuts at: 33 144 346 1247 1813 2135 2195 2276 2718
Size: 111 202 901 566 322 60 81 442
Cuts at: 2718 3403 4398 4589 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 901 685 566 442 322 202
191 111 81 60
SfoI GGC'GCC
Cuts at: 3107 3355 3107
Size: 248 5429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5677
SmaI CCC'GGG
Cuts at: 2697 2697
Size: 5677
SmlI C'TyrA_G
Cuts at: 1375 1625 2237 2830 3745 4239 4501 1375
Size: 250 612 593 915 494 262 2551
Fragments arranged by size:
2551 915 612 593 494 262 250
SnaBI TAC'GTA
Cuts at: 2667 2667
Size: 5677
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5677
SphI G_CATG'C
Cuts at: 2728 3228 2728
Size: 500 5177
SspI AAT'ATT
Cuts at: 676 1746 676
Size: 1070 4607
StyI C'CwwG_G
Cuts at: 1800 2502 2648 2809 3163 1800
Size: 702 146 161 354 4314
Fragments arranged by size:
4314 702 354 161 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1023 1527 2200 3258 3391 3850 4061 1023
Size: 504 673 1058 133 459 211 2639
Fragments arranged by size:
2639 1058 673 504 459 211 133
TatI w'GTAC_w
Cuts at: 1237 2014 2040 2305 5192 1237
Size: 777 26 265 2887 1722
Fragments arranged by size:
2887 1722 777 265 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5194 5226 5194
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 2376 3472 2376
Size: 1096 4581
XbaI T'CTAG_A
Cuts at: 238 958 990 2706 238
Size: 720 32 1716 3209
Fragments arranged by size:
3209 1716 720 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1948 3170 1948
Size: 1222 4455
XmaI C'CCGG_G
Cuts at: 2695 2695
Size: 5677
XmnI GAAnn'nnTTC
Cuts at: 1787 1787
Size: 5677
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI