Restriction map for
pF1KSDA0341
(Circular) (Six-base) MAPSORT of: pF1KSDA0341.seq Check: 9328 from: 1 to: 4771
pF1KSDA0341 4771 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 966 966
Size: 4771
AccI GT'mk_AC
Cuts at: 1807 1807
Size: 4771
Acc65I G'GTAC_C
Cuts at: 1785 1785
Size: 4771
AclI AA'CG_TT
Cuts at: 4611 4611
Size: 4771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 399 732 1036 1305 1348 2594 3026 3775
Size: 175 333 304 269 43 1246 432 749
Cuts at: 3775 4043 224
Size: 268 952
Fragments arranged by size:
1246 952 749 432 333 304 269 268
175 43
AflIII A'CryG_T
Cuts at: 1664 2121 3227 4204 1664
Size: 457 1106 977 2231
Fragments arranged by size:
2231 1106 977 457
AgeI A'CCGG_T
Cuts at: 3159 3159
Size: 4771
AleI CACnn'nnGTG
Cuts at: 976 976
Size: 4771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1150 1495 1150
Size: 345 4426
AlwNI CAG_nnn'CTG
Cuts at: 145 549 639 981 999 1152 1192 1275 1308
Size: 404 90 342 18 153 40 83 33
Cuts at: 1308 1575 1686 1698 2074 3643 145
Size: 267 111 12 376 1569 1273
Fragments arranged by size:
1569 1273 404 376 342 267 153 111
90 83 40 33 18 12
ApaI G_GGCC'C
Cuts at: 351 611 1173 351
Size: 260 562 3949
Fragments arranged by size:
3949 562 260
ApaLI G'TGCA_C
Cuts at: 1202 3541 1202
Size: 2339 2432
ApoI r'AATT_y
Cuts at: 1773 4124 4380 1773
Size: 2351 256 2164
Fragments arranged by size:
2351 2164 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4771
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4771
AvaI C'yCGr_G
Cuts at: 233 604 611 626 676 778 850 913 1166
Size: 371 7 15 50 102 72 63 253
Cuts at: 1166 1427 1741 1789 233
Size: 261 314 48 3215
Fragments arranged by size:
3215 371 314 261 253 102 72 63
50 48 15 7
BamHI G'GATC_C
Cuts at: 1794 1794
Size: 4771
BanI G'GyrC_C
Cuts at: 1118 1177 1785 2199 2447 2482 1118
Size: 59 608 414 248 35 3407
Fragments arranged by size:
3407 608 414 248 59 35
BanII G_rGCy'C
Cuts at: 241 260 351 604 611 677 683 787 932
Size: 19 91 253 7 66 6 104 145
Cuts at: 932 1143 1173 1718 1783 2813 241
Size: 211 30 545 65 1030 2199
Fragments arranged by size:
2199 1030 545 253 211 145 104 91
66 65 30 19 7 6
BbeI G_GCGC'C
Cuts at: 1122 2203 2451 1122
Size: 1081 248 3442
Fragments arranged by size:
3442 1081 248
BbsI GAAGACnn'nnnn_
Cuts at: 1249 1336 1249
Size: 87 4684
BbvCI CC'TCA_GC
Cuts at: 292 649 292
Size: 357 4414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1408 1442 1408
Size: 34 4737
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3430 4771 2667
Size: 763 1341 2667
Fragments arranged by size:
2667 1341 763
BclI T'GATC_A
Cuts at: 2289 2289
Size: 4771
BglI GCCn_nnn'nGGC
Cuts at: 149 255 622 149
Size: 106 367 4298
Fragments arranged by size:
4298 367 106
BglII A'GATC_T
Cuts at: 1316 1754 2284 4323 1316
Size: 438 530 2039 1764
Fragments arranged by size:
2039 1764 530 438
BlpI GC'TnA_GC
Cuts at: 838 1010 1061 1103 1142 1520 1535 1881 838
Size: 172 51 42 39 378 15 346 3728
Fragments arranged by size:
3728 378 346 172 51 42 39 15
Bme1580I G_kGCm'C
Cuts at: 351 611 633 1173 1206 1356 1707 2394 2487
Size: 260 22 540 33 150 351 687 93
Cuts at: 2487 3545 351
Size: 1058 1577
Fragments arranged by size:
1577 1058 687 540 351 260 150 93
33 22
BmrI ACTGGGnnnn_n'
Cuts at: 1193 1981 2062 2397 4543 1193
Size: 788 81 335 2146 1421
Fragments arranged by size:
2146 1421 788 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 975 3136 975
Size: 2161 2610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1003 1066 1708 1749 684
Size: 319 63 642 41 3706
Fragments arranged by size:
3706 642 319 63 41
Bpu10I CC'TnA_GC
Cuts at: 292 598 649 671 1367 292
Size: 306 51 22 696 3696
Fragments arranged by size:
3696 696 306 51 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 916 1945 2824 3318 3616 788
Size: 128 1029 879 494 298 1943
Fragments arranged by size:
1943 1029 879 494 298 128
BsaI GGTCTCn'nnnn_
Cuts at: 236 910 4475 236
Size: 674 3565 532
Fragments arranged by size:
3565 674 532
BsaAI yAC'GTr
Cuts at: 1761 2122 1761
Size: 361 4410
BsaBI GATnn'nnATC
Cuts at: 6 2283 4322 6
Size: 2277 2039 455
Fragments arranged by size:
2277 2039 455
BsaHI Gr'CG_yC
Cuts at: 529 1119 2200 2448 3152 4648 529
Size: 590 1081 248 704 1496 652
Fragments arranged by size:
1496 1081 704 652 590 248
BsaWI w'CCGG_w
Cuts at: 263 1158 1958 2479 3159 3433 3580 3991 263
Size: 895 800 521 680 274 147 411 1043
Fragments arranged by size:
1043 895 800 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 859 889 859
Size: 30 4741
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 687 744 873 940 1000 1228 1624 250
Size: 437 57 129 67 60 228 396 3397
Fragments arranged by size:
3397 437 396 228 129 67 60 57
BseYI C'CCAG_C
Cuts at: 682 820 2194 2952 3531 4027 682
Size: 138 1374 758 579 496 1426
Fragments arranged by size:
1426 1374 758 579 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 556 625 1024 286
Size: 270 69 399 4033
Fragments arranged by size:
4033 399 270 69
BsiEI CG_ry'CG
Cuts at: 104 2311 2357 3567 104
Size: 2207 46 1210 1308
Fragments arranged by size:
2207 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 331 932 1206 1783 2561 2751 3545 331
Size: 601 274 577 778 190 794 1557
Fragments arranged by size:
1557 794 778 601 577 274 190
BsmI GAATG_Cn'
Cuts at: 461 461
Size: 4771
Bsp1286I G_dGCh'C
Cuts at: 241 260 331 351 604 611 633 677 683
Size: 19 71 20 253 7 22 44 6
Cuts at: 683 787 932 1143 1173 1206 1356 1707 1718
Size: 104 145 211 30 33 150 351 11
Cuts at: 1718 1783 2394 2487 2561 2751 2813 3545 241
Size: 65 611 93 74 190 62 732 1467
Fragments arranged by size:
1467 732 611 351 253 211 190 150
145 104 93 74 71 65 62 44
33 30 22 20 19 11 7 6
BspEI T'CCGG_A
Cuts at: 263 1158 3991 263
Size: 895 2833 1043
Fragments arranged by size:
2833 1043 895
BspHI T'CATG_A
Cuts at: 3947 3947
Size: 4771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 966 1819 2033 2335 2716 308
Size: 658 853 214 302 381 2363
Fragments arranged by size:
2363 853 658 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1035 1593 3061 3115 4601 4766 50
Size: 985 558 1468 54 1486 165 55
Fragments arranged by size:
1486 1468 985 558 165 55 54
BsrDI GCAATG_nn'
Cuts at: 152 567 2681 152
Size: 415 2114 2242
Fragments arranged by size:
2242 2114 415
BsrFI r'CCGG_y
Cuts at: 2767 2948 3159 2767
Size: 181 211 4379
Fragments arranged by size:
4379 211 181
BssSI C'ACGA_G
Cuts at: 1042 3040 3400 1042
Size: 1998 360 2413
Fragments arranged by size:
2413 1998 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1275 1575 1698 2146 1275
Size: 300 123 448 3900
Fragments arranged by size:
3900 448 300 123
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4771
BstEII G'GTnAC_C
Cuts at: 1047 1047
Size: 4771
BstXI CCAn_nnnn'nTGG
Cuts at: 253 253
Size: 4771
BstYI r'GATC_y
Cuts at: 1 1316 1643 1754 1794 2261 2284 2619 2865
Size: 1315 327 111 40 467 23 335 246
Cuts at: 2865 3879 3965 3977 4323 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1315 1014 467 449 346 335 327 246
111 86 40 23 12
BtgI C'CryG_G
Cuts at: 125 158 211 125
Size: 33 53 4685
Fragments arranged by size:
4685 53 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 962 2719 2900 3063 962
Size: 1757 181 163 2670
Fragments arranged by size:
2670 1757 181 163
BtsI GCAGTG_nn'
Cuts at: 1705 2012 1705
Size: 307 4464
DraI TTT'AAA
Cuts at: 1768 1768
Size: 4771
DraIII CAC_nnn'GTG
Cuts at: 1047 1047
Size: 4771
DrdI GACnn_nn'nnGTC
Cuts at: 2475 3335 2475
Size: 860 3911
EaeI y'GGCC_r
Cuts at: 128 762 2354 2528 2919 2946 4293 128
Size: 634 1592 174 391 27 1347 606
Fragments arranged by size:
1592 1347 634 606 391 174 27
EagI C'GGCC_G
Cuts at: 2354 2354
Size: 4771
EarI CTCTTCn'nnn_
Cuts at: 200 2792 3002 200
Size: 2592 210 1969
Fragments arranged by size:
2592 1969 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1306 1327 1611 2977 3289 3435 4581 1306
Size: 21 284 1366 312 146 1146 1496
Fragments arranged by size:
1496 1366 1146 312 284 146 21
EcoICRI GAG'CTC
Cuts at: 930 1781 930
Size: 851 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 399 684 732 1003 1036 1066 1305 1348
Size: 175 285 48 271 33 30 239 43
Cuts at: 1348 1708 1749 2594 3026 3775 4043 224
Size: 360 41 845 432 749 268 952
Fragments arranged by size:
952 845 749 432 360 285 271 268
239 175 48 43 41 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 265 658 1126 1186 265
Size: 393 468 60 3850
Fragments arranged by size:
3850 468 393 60
EcoO109I rG'GnC_Cy
Cuts at: 142 348 608 808 939 1149 1170 1422 1476
Size: 206 260 200 131 210 21 252 54
Cuts at: 1476 1908 142
Size: 432 3005
Fragments arranged by size:
3005 432 260 252 210 206 200 131
54 21
EcoRI G'AATT_C
Cuts at: 1773 1773
Size: 4771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 4771
FspI TGC'GCA
Cuts at: 1126 1405 2550 1126
Size: 279 1145 3347
Fragments arranged by size:
3347 1145 279
HaeII r_GCGC'y
Cuts at: 993 1023 1122 1576 2203 2451 3475 4454 993
Size: 30 99 454 627 248 1024 979 1310
Fragments arranged by size:
1310 1024 979 627 454 248 99 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 784 816 1308 1340 2611 2641 2643 2673 784
Size: 32 492 32 1271 30 2 30 2882
Fragments arranged by size:
2882 1271 492 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1808 1808
Size: 4771
KasI G'GCGC_C
Cuts at: 1118 2199 2447 1118
Size: 1081 248 3442
Fragments arranged by size:
3442 1081 248
KpnI G_GTAC'C
Cuts at: 1789 1789
Size: 4771
MluI A'CGCG_T
Cuts at: 4204 4204
Size: 4771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 716 986 3442 3626 161
Size: 555 270 2456 184 1306
Fragments arranged by size:
2456 1306 555 270 184
MscI TGG'CCA
Cuts at: 130 764 2530 130
Size: 634 1766 2371
Fragments arranged by size:
2371 1766 634
MslI CAynn'nnrTG
Cuts at: 157 976 1663 2758 2885 157
Size: 819 687 1095 127 2043
Fragments arranged by size:
2043 1095 819 687 127
MspA1I CmG'CkG
Cuts at: 213 636 832 996 1086 1092 1116 1275 1448
Size: 423 196 164 90 6 24 159 173
Cuts at: 1448 1527 1638 1880 2194 2554 3569 3814 213
Size: 79 111 242 314 360 1015 245 1170
Fragments arranged by size:
1170 1015 423 360 314 245 242 196
173 164 159 111 90 79 24 6
NaeI GCC'GGC
Cuts at: 2950 2950
Size: 4771
NarI GG'CG_CC
Cuts at: 1119 2200 2448 1119
Size: 1081 248 3442
Fragments arranged by size:
3442 1081 248
NcoI C'CATG_G
Cuts at: 125 158 125
Size: 33 4738
NgoMIV G'CCGG_C
Cuts at: 2948 2948
Size: 4771
NruI TCG'CGA
Cuts at: 3132 3132
Size: 4771
NspI r_CATG'y
Cuts at: 1668 1822 2322 3231 1668
Size: 154 500 909 3208
Fragments arranged by size:
3208 909 500 154
PciI A'CATG_T
Cuts at: 1664 3227 1664
Size: 1563 3208
PflMI CCAn_nnn'nTGG
Cuts at: 1308 1686 1727 1308
Size: 378 41 4352
Fragments arranged by size:
4352 378 41
PmeI GTTT'AAAC
Cuts at: 1768 1768
Size: 4771
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3910 3942 3910
Size: 32 4739
PpuMI rG'GwC_Cy
Cuts at: 808 808
Size: 4771
PspOMI G'GGCC_C
Cuts at: 347 607 1169 347
Size: 260 562 3949
Fragments arranged by size:
3949 562 260
PspXI vC'TCGA_Gb
Cuts at: 1741 1741
Size: 4771
PstI C_TGCA'G
Cuts at: 1091 1415 1547 1816 2501 1091
Size: 324 132 269 685 3361
Fragments arranged by size:
3361 685 324 269 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4771
PvuII CAG'CTG
Cuts at: 636 832 1086 1092 1275 1448 1527 2194 2554
Size: 196 254 6 183 173 79 667 360
Cuts at: 2554 636
Size: 2853
Fragments arranged by size:
2853 667 360 254 196 183 173 79
6
RsrII CG'GwC_CG
Cuts at: 2964 2964
Size: 4771
SacI G_AGCT'C
Cuts at: 932 1783 932
Size: 851 3920
SacII CC_GC'GG
Cuts at: 214 214
Size: 4771
SalI G'TCGA_C
Cuts at: 1806 1806
Size: 4771
SapI GCTCTTCn'nnn_
Cuts at: 200 2792 3002 200
Size: 2592 210 1969
Fragments arranged by size:
2592 1969 210
SbfI CC_TGCA'GG
Cuts at: 1415 1816 1415
Size: 401 4370
ScaI AGT'ACT
Cuts at: 4288 4288
Size: 4771
SfcI C'TryA_G
Cuts at: 33 1087 1411 1543 1812 2497 3492 3683 33
Size: 1054 324 132 269 685 995 191 1121
Fragments arranged by size:
1121 1054 995 685 324 269 191 132
SfiI GGCCn_nnn'nGGCC
Cuts at: 622 622
Size: 4771
SfoI GGC'GCC
Cuts at: 1120 2201 2449 1120
Size: 1081 248 3442
Fragments arranged by size:
3442 1081 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4771
SmaI CCC'GGG
Cuts at: 606 613 628 1791 606
Size: 7 15 1163 3586
Fragments arranged by size:
3586 1163 15 7
SmlI C'TyrA_G
Cuts at: 803 931 1741 1924 2839 3333 3595 803
Size: 128 810 183 915 494 262 1979
Fragments arranged by size:
1979 915 810 494 262 183 128
SnaBI TAC'GTA
Cuts at: 1761 1761
Size: 4771
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4771
SphI G_CATG'C
Cuts at: 1822 2322 1822
Size: 500 4271
SrfI GCCC'GGGC
Cuts at: 628 628
Size: 4771
StyI C'CwwG_G
Cuts at: 125 158 759 812 942 1903 2257 125
Size: 33 601 53 130 961 354 2639
Fragments arranged by size:
2639 961 601 354 130 53 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2352 2485 2944 3155 2352
Size: 133 459 211 3968
Fragments arranged by size:
3968 459 211 133
TatI w'GTAC_w
Cuts at: 4286 4286
Size: 4771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4288 4320 4288
Size: 32 4739
Tth111I GACn'n_nGTC
Cuts at: 2566 2566
Size: 4771
XbaI T'CTAG_A
Cuts at: 1800 1800
Size: 4771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 522 2264 165
Size: 357 1742 2672
Fragments arranged by size:
2672 1742 357
XhoI C'TCGA_G
Cuts at: 1741 1741
Size: 4771
XmaI C'CCGG_G
Cuts at: 604 611 626 1789 604
Size: 7 15 1163 3586
Fragments arranged by size:
3586 1163 15 7
XmnI GAAnn'nnTTC
Cuts at: 505 505
Size: 4771
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PmeI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PfoI
PmlI PshAI PsiI PsrI SanDI SexAI SgrAI SspI
StuI SwaI ZraI