Restriction map for
pF1KSDA0352
(Circular) (Six-base) MAPSORT of: pF1KSDA0352.seq Check: 2027 from: 1 to: 5273
pF1KSDA0352 5273 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 221 221
Size: 5273
AccI GT'mk_AC
Cuts at: 386 926 2311 386
Size: 540 1385 3348
Fragments arranged by size:
3348 1385 540
Acc65I G'GTAC_C
Cuts at: 553 2289 553
Size: 1736 3537
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 377 873 3098 3530 4277 4545 196
Size: 181 496 2225 432 747 268 924
Fragments arranged by size:
2225 924 747 496 432 268 181
AflIII A'CryG_T
Cuts at: 1334 2160 2625 3729 4706 1334
Size: 826 465 1104 977 1901
Fragments arranged by size:
1901 1104 977 826 465
AgeI A'CCGG_T
Cuts at: 3661 3661
Size: 5273
AleI CACnn'nnGTG
Cuts at: 226 226
Size: 5273
AlwNI CAG_nnn'CTG
Cuts at: 580 1577 1777 2578 4145 580
Size: 997 200 801 1567 1708
Fragments arranged by size:
1708 1567 997 801 200
ApaI G_GGCC'C
Cuts at: 2100 2100
Size: 5273
ApaLI G'TGCA_C
Cuts at: 1711 2170 4043 1711
Size: 459 1873 2941
Fragments arranged by size:
2941 1873 459
ApoI r'AATT_y
Cuts at: 1981 2277 4626 4882 1981
Size: 296 2349 256 2372
Fragments arranged by size:
2372 2349 296 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5273
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 1012 1214 1283 2091 2293 1012
Size: 202 69 808 202 3992
Fragments arranged by size:
3992 808 202 202 69
AvrII C'CTAG_G
Cuts at: 813 813
Size: 5273
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 537 570 537
Size: 33 5240
BamHI G'GATC_C
Cuts at: 2000 2298 2000
Size: 298 4975
BanI G'GyrC_C
Cuts at: 553 2289 2703 2951 2986 553
Size: 1736 414 248 35 2840
Fragments arranged by size:
2840 1736 414 248 35
BanII G_rGCy'C
Cuts at: 853 1007 1187 2100 2287 3317 853
Size: 154 180 913 187 1030 2809
Fragments arranged by size:
2809 1030 913 187 180 154
BbeI G_GCGC'C
Cuts at: 2707 2955 2707
Size: 248 5025
BbsI GAAGACnn'nnnn_
Cuts at: 761 1052 1638 1733 761
Size: 291 586 95 4301
Fragments arranged by size:
4301 586 291 95
BbvCI CC'TCA_GC
Cuts at: 1055 1055
Size: 5273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1195 1229 1195
Size: 34 5239
BciVI GTATCCnnnnn_n'
Cuts at: 3171 3932 5273 3171
Size: 761 1341 3171
Fragments arranged by size:
3171 1341 761
BclI T'GATC_A
Cuts at: 413 2793 413
Size: 2380 2893
BglI GCCn_nnn'nGGC
Cuts at: 259 259
Size: 5273
BglII A'GATC_T
Cuts at: 2788 4825 2788
Size: 2037 3236
BlpI GC'TnA_GC
Cuts at: 443 2385 443
Size: 1942 3331
Bme1580I G_kGCm'C
Cuts at: 1715 2100 2129 2174 2898 2991 4047 1715
Size: 385 29 45 724 93 1056 2941
Fragments arranged by size:
2941 1056 724 385 93 45 29
BmgBI CAC'GTC
Cuts at: 782 1794 782
Size: 1012 4261
BmrI ACTGGGnnnn_n'
Cuts at: 320 644 947 1353 2485 2566 2901 5045 320
Size: 324 303 406 1132 81 335 2144 548
Fragments arranged by size:
2144 1132 548 406 335 324 303 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 564 3640 564
Size: 3076 2197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 520 680 991 1846 454
Size: 66 160 311 855 3881
Fragments arranged by size:
3881 855 311 160 66
Bpu10I CC'TnA_GC
Cuts at: 1055 1055
Size: 5273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3328 3820 4118 2449
Size: 879 492 298 3604
Fragments arranged by size:
3604 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 199 1252 1453 4977 199
Size: 1053 201 3524 495
Fragments arranged by size:
3524 1053 495 201
BsaAI yAC'GTr
Cuts at: 2265 2626 2265
Size: 361 4912
BsaBI GATnn'nnATC
Cuts at: 6 2787 4824 6
Size: 2781 2037 455
Fragments arranged by size:
2781 2037 455
BsaHI Gr'CG_yC
Cuts at: 218 1730 2704 2952 3654 5150 218
Size: 1512 974 248 702 1496 341
Fragments arranged by size:
1512 1496 974 702 341 248
BsaWI w'CCGG_w
Cuts at: 2462 2983 3661 3935 4082 4493 2462
Size: 521 678 274 147 411 3242
Fragments arranged by size:
3242 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 756 786 1264 1294 756
Size: 30 478 30 4735
Fragments arranged by size:
4735 478 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 1090 1900 457
Size: 633 810 3830
Fragments arranged by size:
3830 810 633
BseYI C'CCAG_C
Cuts at: 803 1443 1854 2698 3456 4033 4529 803
Size: 640 411 844 758 577 496 1547
Fragments arranged by size:
1547 844 758 640 577 496 411
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 1773 2097 296
Size: 1477 324 3472
Fragments arranged by size:
3472 1477 324
BsiEI CG_ry'CG
Cuts at: 104 2815 2861 4069 104
Size: 2711 46 1208 1308
Fragments arranged by size:
2711 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 508 853 971 1007 1715 2174 2287 3065 3255
Size: 345 118 36 708 459 113 778 190
Cuts at: 3255 4047 508
Size: 792 1734
Fragments arranged by size:
1734 792 778 708 459 345 190 118
113 36
Bsp1286I G_dGCh'C
Cuts at: 508 853 971 1007 1187 1715 2100 2129 2174
Size: 345 118 36 180 528 385 29 45
Cuts at: 2174 2287 2898 2991 3065 3255 3317 4047 508
Size: 113 611 93 74 190 62 730 1734
Fragments arranged by size:
1734 730 611 528 385 345 190 180
118 113 93 74 62 45 36 29
BspEI T'CCGG_A
Cuts at: 4493 4493
Size: 5273
BspHI T'CATG_A
Cuts at: 2150 4449 2150
Size: 2299 2974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 588 1238 2323 2537 2839 3220 179
Size: 409 650 1085 214 302 381 2232
Fragments arranged by size:
2232 1085 650 409 381 302 214
BsrBI CCG'CTC
Cuts at: 50 242 2060 3565 3619 5103 5268 50
Size: 192 1818 1505 54 1484 165 55
Fragments arranged by size:
1818 1505 1484 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 885 3185 885
Size: 2300 2973
BsrFI r'CCGG_y
Cuts at: 194 1994 3271 3452 3661 194
Size: 1800 1277 181 209 1806
Fragments arranged by size:
1806 1800 1277 209 181
BsrGI T'GTAC_A
Cuts at: 2219 2219
Size: 5273
BssSI C'ACGA_G
Cuts at: 846 3544 3902 846
Size: 2698 358 2217
Fragments arranged by size:
2698 2217 358
BstAPI GCAn_nnn'nTGC
Cuts at: 580 1253 1577 2650 580
Size: 673 324 1073 3203
Fragments arranged by size:
3203 1073 673 324
BstBI TT'CG_AA
Cuts at: 110 3634 110
Size: 3524 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 231 592 231
Size: 361 4912
BstYI r'GATC_y
Cuts at: 1 978 2000 2298 2765 2788 3123 3369 4381
Size: 977 1022 298 467 23 335 246 1012
Cuts at: 4381 4467 4479 4825 1
Size: 86 12 346 449
Fragments arranged by size:
1022 1012 977 467 449 346 335 298
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3223 3404 3567 3223
Size: 181 163 4929
Fragments arranged by size:
4929 181 163
BtsI GCAGTG_nn'
Cuts at: 506 1578 1740 2516 506
Size: 1072 162 776 3263
Fragments arranged by size:
3263 1072 776 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1935 1970 1935
Size: 35 5238
DraI TTT'AAA
Cuts at: 2272 2272
Size: 5273
DrdI GACnn_nn'nnGTC
Cuts at: 2979 3837 2979
Size: 858 4415
EaeI y'GGCC_r
Cuts at: 1505 2858 3032 3423 3450 4795 1505
Size: 1353 174 391 27 1345 1983
Fragments arranged by size:
1983 1353 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2858 2858
Size: 5273
EarI CTCTTCn'nnn_
Cuts at: 304 406 674 1741 1895 3296 3506 304
Size: 102 268 1067 154 1401 210 2071
Fragments arranged by size:
2071 1401 1067 268 210 154 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2013 2257 3481 3791 3937 5083 2013
Size: 244 1224 310 146 1146 2203
Fragments arranged by size:
2203 1224 1146 310 244 146
EcoICRI GAG'CTC
Cuts at: 851 1005 2285 851
Size: 154 1280 3839
Fragments arranged by size:
3839 1280 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 377 454 520 680 873 991 1846 3098
Size: 181 77 66 160 193 118 855 1252
Cuts at: 3098 3530 4277 4545 196
Size: 432 747 268 924
Fragments arranged by size:
1252 924 855 747 432 268 193 181
160 118 77 66
EcoNI CCTnn'n_nnAGG
Cuts at: 175 469 175
Size: 294 4979
EcoO109I rG'GnC_Cy
Cuts at: 329 462 1017 2096 2097 2412 329
Size: 133 555 1079 1 315 3190
Fragments arranged by size:
3190 1079 555 315 133 1
EcoRI G'AATT_C
Cuts at: 1981 2277 1981
Size: 296 4977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 5273
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 1912 2707 2955 3977 4956 1912
Size: 795 248 1022 979 2229
Fragments arranged by size:
2229 1022 979 795 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1264 1296 3115 3145 3147 3177 1264
Size: 32 1819 30 2 30 3360
Fragments arranged by size:
3360 1819 32 30 30 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5273
KasI G'GCGC_C
Cuts at: 2703 2951 2703
Size: 248 5025
KpnI G_GTAC'C
Cuts at: 557 2293 557
Size: 1736 3537
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3944 4128 3944
Size: 184 5089
MscI TGG'CCA
Cuts at: 1507 3034 1507
Size: 1527 3746
MslI CAynn'nnrTG
Cuts at: 130 226 229 1708 2025 2165 3262 3389 130
Size: 96 3 1479 317 140 1097 127 2014
Fragments arranged by size:
2014 1479 1097 317 140 127 96 3
MspA1I CmG'CkG
Cuts at: 280 1368 2111 2384 2698 3058 4071 4316 280
Size: 1088 743 273 314 360 1013 245 1237
Fragments arranged by size:
1237 1088 1013 743 360 314 273 245
NaeI GCC'GGC
Cuts at: 196 3454 196
Size: 3258 2015
NarI GG'CG_CC
Cuts at: 2704 2952 2704
Size: 248 5025
NcoI C'CATG_G
Cuts at: 125 125
Size: 5273
NgoMIV G'CCGG_C
Cuts at: 194 3452 194
Size: 3258 2015
NspI r_CATG'y
Cuts at: 1213 1338 2164 2326 2826 3733 1213
Size: 125 826 162 500 907 2753
Fragments arranged by size:
2753 907 826 500 162 125
PciI A'CATG_T
Cuts at: 1334 2160 3729 1334
Size: 826 1569 2878
Fragments arranged by size:
2878 1569 826
PflMI CCAn_nnn'nTGG
Cuts at: 1722 1722
Size: 5273
PfoI T'CCnGG_A
Cuts at: 1268 1268
Size: 5273
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 4412
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 329 462 1017 329
Size: 133 555 4585
Fragments arranged by size:
4585 555 133
PshAI GACnn'nnGTC
Cuts at: 1586 1586
Size: 5273
PspOMI G'GGCC_C
Cuts at: 2096 2096
Size: 5273
PspXI vC'TCGA_Gb
Cuts at: 1012 1012
Size: 5273
PstI C_TGCA'G
Cuts at: 1573 1606 1850 1916 2320 3005 1573
Size: 33 244 66 404 685 3841
Fragments arranged by size:
3841 685 404 244 66 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 280 1368 2698 3058 280
Size: 1088 1330 360 2495
Fragments arranged by size:
2495 1330 1088 360
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 853 1007 2287 853
Size: 154 1280 3839
Fragments arranged by size:
3839 1280 154
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 1895 3296 3506 1895
Size: 1401 210 3662
Fragments arranged by size:
3662 1401 210
SbfI CC_TGCA'GG
Cuts at: 2320 2320
Size: 5273
ScaI AGT'ACT
Cuts at: 4790 4790
Size: 5273
SfcI C'TryA_G
Cuts at: 33 1569 1602 1846 1912 2130 2316 3001 3994
Size: 1536 33 244 66 218 186 685 993
Cuts at: 3994 4185 33
Size: 191 1121
Fragments arranged by size:
1536 1121 993 685 244 218 191 186
66 33
SfoI GGC'GCC
Cuts at: 2705 2953 2705
Size: 248 5025
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SgrAI Cr'CCGG_yG
Cuts at: 1994 1994
Size: 5273
SmaI CCC'GGG
Cuts at: 1285 2295 1285
Size: 1010 4263
SmlI C'TyrA_G
Cuts at: 1012 2428 3343 3835 4097 1012
Size: 1416 915 492 262 2188
Fragments arranged by size:
2188 1416 915 492 262
SnaBI TAC'GTA
Cuts at: 2265 2265
Size: 5273
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 1213 2326 2826 1213
Size: 1113 500 3660
Fragments arranged by size:
3660 1113 500
SspI AAT'ATT
Cuts at: 1394 1394
Size: 5273
StuI AGG'CCT
Cuts at: 475 1567 475
Size: 1092 4181
StyI C'CwwG_G
Cuts at: 125 591 621 813 2407 2761 125
Size: 466 30 192 1594 354 2637
Fragments arranged by size:
2637 1594 466 354 192 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1289 1868 1885 2856 2989 3448 3657 1289
Size: 579 17 971 133 459 209 2905
Fragments arranged by size:
2905 971 579 459 209 133 17
TatI w'GTAC_w
Cuts at: 2219 4788 2219
Size: 2569 2704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 756 771 788 803 1609 1641 2119 2151 4790
Size: 15 17 15 806 32 478 32 2639
Cuts at: 4790 4822 756
Size: 32 1207
Fragments arranged by size:
2639 1207 806 478 32 32 32 17
15 15
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5273
XbaI T'CTAG_A
Cuts at: 1179 1692 2304 1179
Size: 513 612 4148
Fragments arranged by size:
4148 612 513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 2768 333
Size: 2435 2838
XhoI C'TCGA_G
Cuts at: 1012 1012
Size: 5273
XmaI C'CCGG_G
Cuts at: 1283 2293 1283
Size: 1010 4263
XmnI GAAnn'nnTTC
Cuts at: 298 298
Size: 5273
ZraI GAC'GTC
Cuts at: 219 219
Size: 5273
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI BfrBI
BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PsiI PsrI SacII SanDI SexAI SfiI SrfI SwaI