Restriction map for
pF1KSDA0353
(Circular) (Six-base) MAPSORT of: pF1KSDA0353.seq Check: 7603 from: 1 to: 7834
pF1KSDA0353 7834 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1011 1558 2578 1011
Size: 547 1020 6267
Fragments arranged by size:
6267 1020 547
AccI GT'mk_AC
Cuts at: 4209 4733 4870 4209
Size: 524 137 7173
Fragments arranged by size:
7173 524 137
Acc65I G'GTAC_C
Cuts at: 3895 4848 3895
Size: 953 6881
AclI AA'CG_TT
Cuts at: 7674 7674
Size: 7834
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 3149 3303 3338 3542 5657 6089 6838 7106
Size: 1063 154 35 204 2115 432 749 268
Cuts at: 7106 2086
Size: 2814
Fragments arranged by size:
2814 2115 1063 749 432 268 204 154
35
AflIII A'CryG_T
Cuts at: 200 5184 6290 7267 200
Size: 4984 1106 977 767
Fragments arranged by size:
4984 1106 977 767
AgeI A'CCGG_T
Cuts at: 1945 6222 1945
Size: 4277 3557
AhdI GACnn_n'nnGTC
Cuts at: 4740 4740
Size: 7834
AleI CACnn'nnGTG
Cuts at: 2134 2134
Size: 7834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1395 1427 1395
Size: 32 7802
AlwNI CAG_nnn'CTG
Cuts at: 336 417 2970 3265 3474 3739 5137 6706 336
Size: 81 2553 295 209 265 1398 1569 1464
Fragments arranged by size:
2553 1569 1464 1398 295 265 209 81
ApaI G_GGCC'C
Cuts at: 154 3418 3841 4490 154
Size: 3264 423 649 3498
Fragments arranged by size:
3498 3264 649 423
ApaLI G'TGCA_C
Cuts at: 673 4153 4177 6604 673
Size: 3480 24 2427 1903
Fragments arranged by size:
3480 2427 1903 24
ApoI r'AATT_y
Cuts at: 1132 2869 2920 4836 7187 7443 1132
Size: 1737 51 1916 2351 256 1523
Fragments arranged by size:
2351 1916 1737 1523 256 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 7834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7834
AvaI C'yCGr_G
Cuts at: 154 514 850 1326 2641 3007 3335 3393 4203
Size: 360 336 476 1315 366 328 58 810
Cuts at: 4203 4852 154
Size: 649 3136
Fragments arranged by size:
3136 1315 810 649 476 366 360 336
328 58
AvrII C'CTAG_G
Cuts at: 3693 3693
Size: 7834
BamHI G'GATC_C
Cuts at: 4857 4857
Size: 7834
BanI G'GyrC_C
Cuts at: 1265 1854 1987 3895 4848 5262 5510 5545 1265
Size: 589 133 1908 953 414 248 35 3554
Fragments arranged by size:
3554 1908 953 589 414 248 133 35
BanII G_rGCy'C
Cuts at: 154 170 179 1327 2381 2960 3418 3616 3730
Size: 16 9 1148 1054 579 458 198 114
Cuts at: 3730 3841 4168 4490 4569 4846 5876 154
Size: 111 327 322 79 277 1030 2112
Fragments arranged by size:
2112 1148 1054 1030 579 458 327 322
277 198 114 111 79 16 9
BbeI G_GCGC'C
Cuts at: 5266 5514 5266
Size: 248 7586
BbsI GAAGACnn'nnnn_
Cuts at: 1033 3746 1033
Size: 2713 5121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 482 1556 1590 1893 1927 2725 2759 3710
Size: 34 1074 34 303 34 798 34 951
Cuts at: 3710 3744 448
Size: 34 4538
Fragments arranged by size:
4538 1074 951 798 303 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 908 1884 2071 5730 6493 7834 908
Size: 976 187 3659 763 1341 908
Fragments arranged by size:
3659 1341 976 908 763 187
BclI T'GATC_A
Cuts at: 5352 5352
Size: 7834
BfrBI ATG'CAT
Cuts at: 618 618
Size: 7834
BglI GCCn_nnn'nGGC
Cuts at: 313 517 3452 4074 313
Size: 204 2935 622 4073
Fragments arranged by size:
4073 2935 622 204
BglII A'GATC_T
Cuts at: 5347 7386 5347
Size: 2039 5795
BlpI GC'TnA_GC
Cuts at: 346 1106 3181 4167 4944 346
Size: 760 2075 986 777 3236
Fragments arranged by size:
3236 2075 986 777 760
Bme1580I G_kGCm'C
Cuts at: 154 677 1268 1288 1990 2651 3418 3841 4157
Size: 523 591 20 702 661 767 423 316
Cuts at: 4157 4181 4490 5457 5550 6608 154
Size: 24 309 967 93 1058 1380
Fragments arranged by size:
1380 1058 967 767 702 661 591 523
423 316 309 93 24 20
BmgBI CAC'GTC
Cuts at: 579 579
Size: 7834
BmrI ACTGGGnnnn_n'
Cuts at: 3412 3456 4132 5044 5125 5460 7606 3412
Size: 44 676 912 81 335 2146 3640
Fragments arranged by size:
3640 2146 912 676 335 81 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1836 6199 1836
Size: 4363 3471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 241 262 772 898 973 1063 2069 2612
Size: 87 21 510 126 75 90 1006 543
Cuts at: 2612 2839 3013 3193 3496 3549 3904 4614 4705
Size: 227 174 180 303 53 355 710 91
Cuts at: 4705 154
Size: 3283
Fragments arranged by size:
3283 1006 710 543 510 355 303 227
180 174 126 91 90 87 75 53
21
Bpu10I CC'TnA_GC
Cuts at: 1808 3241 4433 4468 1808
Size: 1433 1192 35 5174
Fragments arranged by size:
5174 1433 1192 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 5008 5887 6381 6679 1430
Size: 3578 879 494 298 2585
Fragments arranged by size:
3578 2585 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4655 7538 4655
Size: 2883 4951
BsaAI yAC'GTr
Cuts at: 201 2136 2568 4824 5185 201
Size: 1935 432 2256 361 2850
Fragments arranged by size:
2850 2256 1935 432 361
BsaBI GATnn'nnATC
Cuts at: 6 1096 2686 3158 5346 7385 6
Size: 1090 1590 472 2188 2039 455
Fragments arranged by size:
2188 2039 1590 1090 472 455
BsaHI Gr'CG_yC
Cuts at: 455 1918 5263 5511 6215 7711 455
Size: 1463 3345 248 704 1496 578
Fragments arranged by size:
3345 1496 1463 704 578 248
BsaWI w'CCGG_w
Cuts at: 1945 3619 5021 5542 6222 6496 6643 7054 1945
Size: 1674 1402 521 680 274 147 411 2725
Fragments arranged by size:
2725 1674 1402 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1395 1425 2067 2097 3013 3043 3995 4025 1395
Size: 30 642 30 916 30 952 30 5204
Fragments arranged by size:
5204 952 916 642 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 262 325 442 769 919 940 1738 2389
Size: 3 63 117 327 150 21 798 651
Cuts at: 2389 2425 2452 2497 2707 2937 3037 3190 4765
Size: 36 27 45 210 230 100 153 1575
Cuts at: 4765 4807 4810 4813 259
Size: 42 3 3 3280
Fragments arranged by size:
3280 1575 798 651 327 230 210 153
150 117 100 63 45 42 36 27
21 3 3 3
BseYI C'CCAG_C
Cuts at: 350 462 495 525 1352 2805 3258 3651 3744
Size: 112 33 30 827 1453 453 393 93
Cuts at: 3744 4088 4651 5257 6015 6594 7090 350
Size: 344 563 606 758 579 496 1094
Fragments arranged by size:
1453 1094 827 758 606 579 563 496
453 393 344 112 93 33 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 2755 2789 4298 4735 742
Size: 2013 34 1509 437 3841
Fragments arranged by size:
3841 2013 1509 437 34
BsiEI CG_ry'CG
Cuts at: 104 261 447 3162 3620 5374 5420 6630 104
Size: 157 186 2715 458 1754 46 1210 1308
Fragments arranged by size:
2715 1754 1308 1210 458 186 157 46
BsiHKAI G_wGCw'C
Cuts at: 170 179 677 1112 4150 4157 4181 4237 4569
Size: 9 498 435 3038 7 24 56 332
Cuts at: 4569 4846 5624 5814 6608 170
Size: 277 778 190 794 1396
Fragments arranged by size:
3038 1396 794 778 498 435 332 277
190 56 24 9 7
BsmI GAATG_Cn'
Cuts at: 3311 3311
Size: 7834
BsmBI CGTCTCn'nnnn_
Cuts at: 709 796 3144 4592 709
Size: 87 2348 1448 3951
Fragments arranged by size:
3951 2348 1448 87
Bsp1286I G_dGCh'C
Cuts at: 154 170 179 677 1112 1268 1288 1327 1990
Size: 16 9 498 435 156 20 39 663
Cuts at: 1990 2381 2651 2960 3418 3616 3730 3841 4150
Size: 391 270 309 458 198 114 111 309
Cuts at: 4150 4157 4168 4181 4237 4490 4569 4846 5457
Size: 7 11 13 56 253 79 277 611
Cuts at: 5457 5550 5624 5814 5876 6608 154
Size: 93 74 190 62 732 1380
Fragments arranged by size:
1380 732 663 611 498 458 435 391
309 309 277 270 253 198 190 156
114 111 93 79 74 62 56 39
20 16 13 11 9 7
BspEI T'CCGG_A
Cuts at: 7054 7054
Size: 7834
BspHI T'CATG_A
Cuts at: 7010 7010
Size: 7834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1011 1558 2578 4882 5096 5398 5779 1011
Size: 547 1020 2304 214 302 381 3066
Fragments arranged by size:
3066 2304 1020 547 381 302 214
BsrBI CCG'CTC
Cuts at: 50 378 930 3292 6124 6178 7664 7829 50
Size: 328 552 2362 2832 54 1486 165 55
Fragments arranged by size:
2832 2362 1486 552 328 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4373 4475 5744 4373
Size: 102 1269 6463
Fragments arranged by size:
6463 1269 102
BsrFI r'CCGG_y
Cuts at: 260 637 1030 1945 3629 5830 6011 6222 260
Size: 377 393 915 1684 2201 181 211 1872
Fragments arranged by size:
2201 1872 1684 915 393 377 211 181
BsrGI T'GTAC_A
Cuts at: 3344 4011 3344
Size: 667 7167
BssHII G'CGCG_C
Cuts at: 316 432 500 502 504 591 593 595 597
Size: 116 68 2 2 87 2 2 2
Cuts at: 597 815 867 869 316
Size: 218 52 2 7281
Fragments arranged by size:
7281 218 116 87 68 52 2 2
2 2 2 2
BssSI C'ACGA_G
Cuts at: 1638 6103 6463 1638
Size: 4465 360 3009
Fragments arranged by size:
4465 3009 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 5209 1570
Size: 3639 4195
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7834
BstEII G'GTnAC_C
Cuts at: 3592 3592
Size: 7834
BstXI CCAn_nnnn'nTGG
Cuts at: 363 2465 3100 3204 3722 4627 363
Size: 2102 635 104 518 905 3570
Fragments arranged by size:
3570 2102 905 635 518 104
BstYI r'GATC_y
Cuts at: 1 1273 2681 4321 4857 5324 5347 5682 5928
Size: 1272 1408 1640 536 467 23 335 246
Cuts at: 5928 6942 7028 7040 7386 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1640 1408 1272 1014 536 467 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 441 983 1269 3074 4104 4383 4584 441
Size: 542 286 1805 1030 279 201 3691
Fragments arranged by size:
3691 1805 1030 542 286 279 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2519 5782 5963 6126 2519
Size: 3263 181 163 4227
Fragments arranged by size:
4227 3263 181 163
BtsI GCAGTG_nn'
Cuts at: 356 688 2777 3560 5075 356
Size: 332 2089 783 1515 3115
Fragments arranged by size:
3115 2089 1515 783 332
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2874 2909 3438 3473 2874
Size: 35 529 35 7235
Fragments arranged by size:
7235 529 35 35
DraI TTT'AAA
Cuts at: 4831 4831
Size: 7834
DrdI GACnn_nn'nnGTC
Cuts at: 2125 5538 6398 2125
Size: 3413 860 3561
Fragments arranged by size:
3561 3413 860
EaeI y'GGCC_r
Cuts at: 258 444 698 771 980 1343 3425 5417 5591
Size: 186 254 73 209 363 2082 1992 174
Cuts at: 5591 5982 6009 7356 258
Size: 391 27 1347 736
Fragments arranged by size:
2082 1992 1347 736 391 363 254 209
186 174 73 27
EagI C'GGCC_G
Cuts at: 258 444 5417 258
Size: 186 4973 2675
Fragments arranged by size:
4973 2675 186
EarI CTCTTCn'nnn_
Cuts at: 794 1305 1682 1828 1881 3878 5855 6065 794
Size: 511 377 146 53 1997 1977 210 2563
Fragments arranged by size:
2563 1997 1977 511 377 210 146 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 382 808 3021 6040 6352 6498 7644 382
Size: 426 2213 3019 312 146 1146 572
Fragments arranged by size:
3019 2213 1146 572 426 312 146
EcoICRI GAG'CTC
Cuts at: 168 177 4567 4844 168
Size: 9 4390 277 3158
Fragments arranged by size:
4390 3158 277 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 241 262 772 898 973 1063 2069 2086
Size: 87 21 510 126 75 90 1006 17
Cuts at: 2086 2612 2839 3013 3149 3193 3303 3338 3496
Size: 526 227 174 136 44 110 35 158
Cuts at: 3496 3542 3549 3904 4614 4705 5657 6089 6838
Size: 46 7 355 710 91 952 432 749
Cuts at: 6838 7106 154
Size: 268 882
Fragments arranged by size:
1006 952 882 749 710 526 510 432
355 268 227 174 158 136 126 110
91 90 87 75 46 44 35 21
17 7
EcoNI CCTnn'n_nnAGG
Cuts at: 244 667 1018 3601 3935 4436 244
Size: 423 351 2583 334 501 3642
Fragments arranged by size:
3642 2583 501 423 351 334
EcoO109I rG'GnC_Cy
Cuts at: 151 276 1011 1494 2461 2826 3297 3380 3414
Size: 125 735 483 967 365 471 83 34
Cuts at: 3414 3556 3655 3697 3837 3838 3928 4345 4486
Size: 142 99 42 140 1 90 417 141
Cuts at: 4486 4971 151
Size: 485 3014
Fragments arranged by size:
3014 967 735 485 483 471 417 365
142 141 140 125 99 90 83 42
34 1
EcoRI G'AATT_C
Cuts at: 1132 2920 4836 1132
Size: 1788 1916 4130
Fragments arranged by size:
4130 1916 1788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4254 5851 4254
Size: 1597 6237
FspI TGC'GCA
Cuts at: 470 5613 470
Size: 5143 2691
HaeII r_GCGC'y
Cuts at: 417 496 523 549 763 835 850 2812 3265
Size: 79 27 26 214 72 15 1962 453
Cuts at: 3265 5266 5514 6538 7517 417
Size: 2001 248 1024 979 734
Fragments arranged by size:
2001 1962 1024 979 734 453 248 214
79 72 27 26 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2297 2329 2336 2368 2650 2682 2683 2715 3013
Size: 32 7 32 282 32 1 32 298
Cuts at: 3013 3045 3529 3561 3671 3703 4726 4758 5674
Size: 32 484 32 110 32 1023 32 916
Cuts at: 5674 5704 5706 5736 2297
Size: 30 2 30 4395
Fragments arranged by size:
4395 1023 916 484 298 282 110 32
32 32 32 32 32 32 32 30
30 7 2 1
HincII GTy'rAC
Cuts at: 2358 3135 4871 2358
Size: 777 1736 5321
Fragments arranged by size:
5321 1736 777
KasI G'GCGC_C
Cuts at: 5262 5510 5262
Size: 248 7586
KpnI G_GTAC'C
Cuts at: 3899 4852 3899
Size: 953 6881
MfeI C'AATT_G
Cuts at: 1523 1523
Size: 7834
MluI A'CGCG_T
Cuts at: 7267 7267
Size: 7834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 836 1438 6505 6689 836
Size: 602 5067 184 1981
Fragments arranged by size:
5067 1981 602 184
MscI TGG'CCA
Cuts at: 982 5593 982
Size: 4611 3223
MslI CAynn'nnrTG
Cuts at: 2134 2932 5821 5948 2134
Size: 798 2889 127 4020
Fragments arranged by size:
4020 2889 798 127
MspA1I CmG'CkG
Cuts at: 302 336 443 726 864 2396 2970 2991 4423
Size: 34 107 283 138 1532 574 21 1432
Cuts at: 4423 4943 5257 5617 6632 6877 302
Size: 520 314 360 1015 245 1259
Fragments arranged by size:
1532 1432 1259 1015 574 520 360 314
283 245 138 107 34 21
NaeI GCC'GGC
Cuts at: 262 6013 262
Size: 5751 2083
NarI GG'CG_CC
Cuts at: 5263 5511 5263
Size: 248 7586
NcoI C'CATG_G
Cuts at: 983 4104 983
Size: 3121 4713
NdeI CA'TA_TG
Cuts at: 3549 3549
Size: 7834
NgoMIV G'CCGG_C
Cuts at: 260 6011 260
Size: 5751 2083
NotI GC'GGCC_GC
Cuts at: 444 444
Size: 7834
NruI TCG'CGA
Cuts at: 6195 6195
Size: 7834
NsiI A_TGCA'T
Cuts at: 620 620
Size: 7834
NspI r_CATG'y
Cuts at: 1125 3646 4885 5385 6294 1125
Size: 2521 1239 500 909 2665
Fragments arranged by size:
2665 2521 1239 909 500
PasI CC'CwG_GG
Cuts at: 2465 3411 2465
Size: 946 6888
PciI A'CATG_T
Cuts at: 6290 6290
Size: 7834
PflMI CCAn_nnn'nTGG
Cuts at: 1420 2843 3265 3739 4110 4728 1420
Size: 1423 422 474 371 618 4526
Fragments arranged by size:
4526 1423 618 474 422 371
PfoI T'CCnGG_A
Cuts at: 170 239 4591 170
Size: 69 4352 3413
Fragments arranged by size:
4352 3413 69
PmeI GTTT'AAAC
Cuts at: 4831 4831
Size: 7834
PmlI CAC'GTG
Cuts at: 2568 2568
Size: 7834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 1427 1681 1713 1960 1992 6973 7005 1395
Size: 32 254 32 247 32 4981 32 2224
Fragments arranged by size:
4981 2224 254 247 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1011 1494 2826 3556 3655 4345 1011
Size: 483 1332 730 99 690 4500
Fragments arranged by size:
4500 1332 730 690 483 99
PshAI GACnn'nnGTC
Cuts at: 3599 3907 4392 3599
Size: 308 485 7041
Fragments arranged by size:
7041 485 308
PspOMI G'GGCC_C
Cuts at: 150 3414 3837 4486 150
Size: 3264 423 649 3498
Fragments arranged by size:
3498 3264 649 423
PspXI vC'TCGA_Gb
Cuts at: 514 514
Size: 7834
PstI C_TGCA'G
Cuts at: 146 413 488 539 1022 3567 3762 3802 3855
Size: 267 75 51 483 2545 195 40 53
Cuts at: 3855 4879 5564 146
Size: 1024 685 2416
Fragments arranged by size:
2545 2416 1024 685 483 267 195 75
53 51 40
PvuI CG_AT'CG
Cuts at: 104 3162 104
Size: 3058 4776
PvuII CAG'CTG
Cuts at: 336 726 864 2970 2991 5257 5617 336
Size: 390 138 2106 21 2266 360 2553
Fragments arranged by size:
2553 2266 2106 390 360 138 21
RsrII CG'GwC_CG
Cuts at: 6027 6027
Size: 7834
SacI G_AGCT'C
Cuts at: 170 179 4569 4846 170
Size: 9 4390 277 3158
Fragments arranged by size:
4390 3158 277 9
SacII CC_GC'GG
Cuts at: 444 444
Size: 7834
SalI G'TCGA_C
Cuts at: 4869 4869
Size: 7834
SanDI GG'GwC_CC
Cuts at: 3655 3655
Size: 7834
SapI GCTCTTCn'nnn_
Cuts at: 1881 5855 6065 1881
Size: 3974 210 3650
Fragments arranged by size:
3974 3650 210
SbfI CC_TGCA'GG
Cuts at: 1022 4879 1022
Size: 3857 3977
ScaI AGT'ACT
Cuts at: 7351 7351
Size: 7834
SfcI C'TryA_G
Cuts at: 33 142 409 484 535 1018 1171 3563 3758
Size: 109 267 75 51 483 153 2392 195
Cuts at: 3758 3798 3851 4349 4875 5560 6555 6746 33
Size: 40 53 498 526 685 995 191 1121
Fragments arranged by size:
2392 1121 995 685 526 498 483 267
195 191 153 109 75 53 51 40
SfoI GGC'GCC
Cuts at: 5264 5512 5264
Size: 248 7586
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7834
SmaI CCC'GGG
Cuts at: 2643 3337 4854 2643
Size: 694 1517 5623
Fragments arranged by size:
5623 1517 694
SmlI C'TyrA_G
Cuts at: 514 1445 4987 5902 6396 6658 514
Size: 931 3542 915 494 262 1690
Fragments arranged by size:
3542 1690 931 915 494 262
SnaBI TAC'GTA
Cuts at: 4824 4824
Size: 7834
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7834
SphI G_CATG'C
Cuts at: 4885 5385 4885
Size: 500 7334
StuI AGG'CCT
Cuts at: 554 4645 554
Size: 4091 3743
StyI C'CwwG_G
Cuts at: 977 983 2795 2951 3693 4104 4966 5320 977
Size: 6 1812 156 742 411 862 354 3491
Fragments arranged by size:
3491 1812 862 742 411 354 156 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 454 3982 4102 5415 5548 6007 6218 454
Size: 3528 120 1313 133 459 211 2070
Fragments arranged by size:
3528 2070 1313 459 211 133 120
TatI w'GTAC_w
Cuts at: 3344 4011 7349 3344
Size: 667 3338 3829
Fragments arranged by size:
3829 3338 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 3639 3671 3849 3881 7351 7383 2067
Size: 32 1540 32 178 32 3470 32 2518
Fragments arranged by size:
3470 2518 1540 178 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4343 5629 4343
Size: 1286 6548
XbaI T'CTAG_A
Cuts at: 1866 4782 4863 1866
Size: 2916 81 4837
Fragments arranged by size:
4837 2916 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3100 3567 5327 3100
Size: 467 1760 5607
Fragments arranged by size:
5607 1760 467
XhoI C'TCGA_G
Cuts at: 514 514
Size: 7834
XmaI C'CCGG_G
Cuts at: 2641 3335 4852 2641
Size: 694 1517 5623
Fragments arranged by size:
5623 1517 694
XmnI GAAnn'nnTTC
Cuts at: 1456 1696 2071 3002 1456
Size: 240 375 931 6288
Fragments arranged by size:
6288 931 375 240
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI BaeI BbvCI BmtI
BsiWI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI NheI PacI PsiI PsrI SexAI SfiI
SgrAI SrfI SspI SwaI ZraI