Restriction map for
pF1KSDA0355
(Circular) (Six-base) MAPSORT of: pF1KSDA0355.seq Check: 7357 from: 1 to: 6349
pF1KSDA0355 6349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3385 3385
Size: 6349
Acc65I G'GTAC_C
Cuts at: 3363 3363
Size: 6349
AclI AA'CG_TT
Cuts at: 6189 6189
Size: 6349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 809 1198 1325 1694 4172 4604 5353 5621
Size: 398 389 127 369 2478 432 749 268
Cuts at: 5621 411
Size: 1139
Fragments arranged by size:
2478 1139 749 432 398 389 369 268
127
AflII C'TTAA_G
Cuts at: 1222 1222
Size: 6349
AflIII A'CryG_T
Cuts at: 3699 4805 5782 3699
Size: 1106 977 4266
Fragments arranged by size:
4266 1106 977
AgeI A'CCGG_T
Cuts at: 4737 4737
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 1553 1553
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1579 2960 3010 1579
Size: 1381 50 4918
Fragments arranged by size:
4918 1381 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 327 2159 327
Size: 1832 4517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 2884 2916 869
Size: 32 1983 32 4302
Fragments arranged by size:
4302 1983 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1368 2920 3168 3652 5221 1368
Size: 1552 248 484 1569 2496
Fragments arranged by size:
2496 1569 1552 484 248
ApaLI G'TGCA_C
Cuts at: 5119 5119
Size: 6349
ApoI r'AATT_y
Cuts at: 579 598 1950 3351 5702 5958 579
Size: 19 1352 1401 2351 256 970
Fragments arranged by size:
2351 1401 1352 970 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 6349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6349
AvaI C'yCGr_G
Cuts at: 2085 2375 2744 2794 2862 3367 2085
Size: 290 369 50 68 505 5067
Fragments arranged by size:
5067 505 369 290 68 50
BamHI G'GATC_C
Cuts at: 3372 3372
Size: 6349
BanI G'GyrC_C
Cuts at: 1342 1487 2229 2277 2379 3363 3777 4025 4060
Size: 145 742 48 102 984 414 248 35
Cuts at: 4060 1342
Size: 3631
Fragments arranged by size:
3631 984 742 414 248 145 102 48
35
BanII G_rGCy'C
Cuts at: 1142 2119 2653 3361 4391 1142
Size: 977 534 708 1030 3100
Fragments arranged by size:
3100 1030 977 708 534
BbeI G_GCGC'C
Cuts at: 3781 4029 3781
Size: 248 6101
BbsI GAAGACnn'nnnn_
Cuts at: 384 1505 1552 1732 384
Size: 1121 47 180 5001
Fragments arranged by size:
5001 1121 180 47
BbvCI CC'TCA_GC
Cuts at: 719 827 2282 2880 719
Size: 108 1455 598 4188
Fragments arranged by size:
4188 1455 598 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 1362 3187 3221 1328
Size: 34 1825 34 4456
Fragments arranged by size:
4456 1825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4245 5008 6349 4245
Size: 763 1341 4245
Fragments arranged by size:
4245 1341 763
BclI T'GATC_A
Cuts at: 2052 3867 2052
Size: 1815 4534
BglI GCCn_nnn'nGGC
Cuts at: 2735 2735
Size: 6349
BglII A'GATC_T
Cuts at: 420 1655 3862 5901 420
Size: 1235 2207 2039 868
Fragments arranged by size:
2207 2039 1235 868
BlpI GC'TnA_GC
Cuts at: 3459 3459
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 144 2216 2232 2247 2382 3972 4065 5123 144
Size: 2072 16 15 135 1590 93 1058 1370
Fragments arranged by size:
2072 1590 1370 1058 135 93 16 15
BmgBI CAC'GTC
Cuts at: 1566 1566
Size: 6349
BmrI ACTGGGnnnn_n'
Cuts at: 3172 3324 3559 3640 3975 6121 3172
Size: 152 235 81 335 2146 3400
Fragments arranged by size:
3400 2146 335 235 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2895 4714 2895
Size: 1819 4530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 1620 1764 2323 592
Size: 1028 144 559 4618
Fragments arranged by size:
4618 1028 559 144
Bpu10I CC'TnA_GC
Cuts at: 230 719 827 923 996 2282 2843 2880 230
Size: 489 108 96 73 1286 561 37 3699
Fragments arranged by size:
3699 1286 561 489 108 96 73 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 3523 4402 4896 5194 2297
Size: 1226 879 494 298 3452
Fragments arranged by size:
3452 1226 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6053 6053
Size: 6349
BsaAI yAC'GTr
Cuts at: 3339 3700 3339
Size: 361 5988
BsaBI GATnn'nnATC
Cuts at: 6 3861 5900 6
Size: 3855 2039 455
Fragments arranged by size:
3855 2039 455
BsaHI Gr'CG_yC
Cuts at: 1184 3778 4026 4730 6226 1184
Size: 2594 248 704 1496 1307
Fragments arranged by size:
2594 1496 1307 704 248
BsaWI w'CCGG_w
Cuts at: 3536 4057 4737 5011 5158 5569 3536
Size: 521 680 274 147 411 4316
Fragments arranged by size:
4316 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2321 2351 2984 3014 2321
Size: 30 633 30 5656
Fragments arranged by size:
5656 633 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2435 2992 2435
Size: 557 5792
BseYI C'CCAG_C
Cuts at: 234 568 1091 2239 2264 2369 2408 2616 2717
Size: 334 523 1148 25 105 39 208 101
Cuts at: 2717 3088 3100 3106 3239 3772 4530 5109 5605
Size: 371 12 6 133 533 758 579 496
Cuts at: 5605 234
Size: 978
Fragments arranged by size:
1148 978 758 579 533 523 496 371
334 208 133 105 101 39 25 12
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 505 932 1177 1381 2806 3247 223
Size: 282 427 245 204 1425 441 3325
Fragments arranged by size:
3325 1425 441 427 282 245 204
BsiEI CG_ry'CG
Cuts at: 104 1205 2727 3889 3935 5145 104
Size: 1101 1522 1162 46 1210 1308
Fragments arranged by size:
1522 1308 1210 1162 1101 46
BsiHKAI G_wGCw'C
Cuts at: 246 255 929 1142 1571 2844 3361 4139 4329
Size: 9 674 213 429 1273 517 778 190
Cuts at: 4329 5123 246
Size: 794 1472
Fragments arranged by size:
1472 1273 794 778 674 517 429 213
190 9
BsmI GAATG_Cn'
Cuts at: 2618 2618
Size: 6349
BsmBI CGTCTCn'nnnn_
Cuts at: 1191 1191
Size: 6349
Bsp1286I G_dGCh'C
Cuts at: 144 246 255 929 1142 1571 2119 2216 2232
Size: 102 9 674 213 429 548 97 16
Cuts at: 2232 2247 2382 2653 2844 3361 3972 4065 4139
Size: 15 135 271 191 517 611 93 74
Cuts at: 4139 4329 4391 5123 144
Size: 190 62 732 1370
Fragments arranged by size:
1370 732 674 611 548 517 429 271
213 191 190 135 102 97 93 74
62 16 15 9
BspEI T'CCGG_A
Cuts at: 5569 5569
Size: 6349
BspHI T'CATG_A
Cuts at: 5525 5525
Size: 6349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1691 2425 3397 3611 3913 4294 1691
Size: 734 972 214 302 381 3746
Fragments arranged by size:
3746 972 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4639 4693 6179 6344 50
Size: 4589 54 1486 165 55
Fragments arranged by size:
4589 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1022 4259 1022
Size: 3237 3112
BsrFI r'CCGG_y
Cuts at: 696 1024 2729 4345 4526 4737 696
Size: 328 1705 1616 181 211 2308
Fragments arranged by size:
2308 1705 1616 328 211 181
BsrGI T'GTAC_A
Cuts at: 2489 2489
Size: 6349
BssSI C'ACGA_G
Cuts at: 4618 4978 4618
Size: 360 5989
BstAPI GCAn_nnn'nTGC
Cuts at: 201 250 1128 3168 3724 201
Size: 49 878 2040 556 2826
Fragments arranged by size:
2826 2040 878 556 49
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 375 1484 2157 2347 2599 375
Size: 1109 673 190 252 4125
Fragments arranged by size:
4125 1109 673 252 190
BstYI r'GATC_y
Cuts at: 1 420 1637 1655 3372 3839 3862 4197 4443
Size: 419 1217 18 1717 467 23 335 246
Cuts at: 4443 5457 5543 5555 5901 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1717 1217 1014 467 449 419 346 335
246 86 23 18 12
BtgI C'CryG_G
Cuts at: 266 2156 2224 3008 266
Size: 1890 68 784 3607
Fragments arranged by size:
3607 1890 784 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 645 2710 4297 4478 4641 645
Size: 2065 1587 181 163 2353
Fragments arranged by size:
2353 2065 1587 181 163
BtsI GCAGTG_nn'
Cuts at: 144 275 759 1138 2203 2651 2967 3210 3590
Size: 131 484 379 1065 448 316 243 380
Cuts at: 3590 144
Size: 2903
Fragments arranged by size:
2903 1065 484 448 380 379 316 243
131
DraI TTT'AAA
Cuts at: 3346 3346
Size: 6349
DraIII CAC_nnn'GTG
Cuts at: 250 1487 250
Size: 1237 5112
DrdI GACnn_nn'nnGTC
Cuts at: 4053 4913 4053
Size: 860 5489
EaeI y'GGCC_r
Cuts at: 269 524 1202 1374 1967 2731 2755 3049 3932
Size: 255 678 172 593 764 24 294 883
Cuts at: 3932 4106 4497 4524 5871 269
Size: 174 391 27 1347 747
Fragments arranged by size:
1347 883 764 747 678 593 391 294
255 174 172 27 24
EagI C'GGCC_G
Cuts at: 1202 3932 1202
Size: 2730 3619
EarI CTCTTCn'nnn_
Cuts at: 430 1844 4370 4580 430
Size: 1414 2526 210 2199
Fragments arranged by size:
2526 2199 1414 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1099 1119 4555 4867 5013 6159 1099
Size: 20 3436 312 146 1146 1289
Fragments arranged by size:
3436 1289 1146 312 146 20
EcoICRI GAG'CTC
Cuts at: 1140 3359 1140
Size: 2219 4130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 592 809 1198 1325 1620 1694 1764 2323
Size: 181 217 389 127 295 74 70 559
Cuts at: 2323 4172 4604 5353 5621 411
Size: 1849 432 749 268 1139
Fragments arranged by size:
1849 1139 749 559 432 389 295 268
217 181 127 74 70
EcoO109I rG'GnC_Cy
Cuts at: 2560 2917 3486 2560
Size: 357 569 5423
Fragments arranged by size:
5423 569 357
EcoRI G'AATT_C
Cuts at: 598 1950 3351 598
Size: 1352 1401 3596
Fragments arranged by size:
3596 1401 1352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 4366 189
Size: 4177 2172
FspI TGC'GCA
Cuts at: 4128 4128
Size: 6349
HaeII r_GCGC'y
Cuts at: 174 3781 4029 5053 6032 174
Size: 3607 248 1024 979 491
Fragments arranged by size:
3607 1024 979 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 1539 1571 2068 2100 2860 2892 4189
Size: 32 687 32 497 32 760 32 1297
Cuts at: 4189 4219 4221 4251 820
Size: 30 2 30 2918
Fragments arranged by size:
2918 1297 760 687 497 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1398 3386 1398
Size: 1988 4361
HindIII A'AGCT_T
Cuts at: 993 1619 1763 993
Size: 626 144 5579
Fragments arranged by size:
5579 626 144
KasI G'GCGC_C
Cuts at: 3777 4025 3777
Size: 248 6101
KpnI G_GTAC'C
Cuts at: 3367 3367
Size: 6349
MluI A'CGCG_T
Cuts at: 5782 5782
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2409 5020 5204 2409
Size: 2611 184 3554
Fragments arranged by size:
3554 2611 184
MscI TGG'CCA
Cuts at: 1376 1969 3051 4108 1376
Size: 593 1082 1057 3617
Fragments arranged by size:
3617 1082 1057 593
MslI CAynn'nnrTG
Cuts at: 619 1482 1579 2012 2155 2960 3010 3163 4336
Size: 863 97 433 143 805 50 153 1173
Cuts at: 4336 4463 619
Size: 127 2505
Fragments arranged by size:
2505 1173 863 805 433 153 143 127
97 50
MspA1I CmG'CkG
Cuts at: 301 311 749 1245 1365 1579 1598 1644 3211
Size: 10 438 496 120 214 19 46 1567
Cuts at: 3211 3458 3772 4132 5147 5392 301
Size: 247 314 360 1015 245 1258
Fragments arranged by size:
1567 1258 1015 496 438 360 314 247
245 214 120 46 19 10
NaeI GCC'GGC
Cuts at: 1026 2731 4528 1026
Size: 1705 1797 2847
Fragments arranged by size:
2847 1797 1705
NarI GG'CG_CC
Cuts at: 3778 4026 3778
Size: 248 6101
NcoI C'CATG_G
Cuts at: 2156 2156
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 1024 2729 4526 1024
Size: 1705 1797 2847
Fragments arranged by size:
2847 1797 1705
NruI TCG'CGA
Cuts at: 4710 4710
Size: 6349
NspI r_CATG'y
Cuts at: 496 3400 3900 4809 496
Size: 2904 500 909 2036
Fragments arranged by size:
2904 2036 909 500
PasI CC'CwG_GG
Cuts at: 285 1551 3295 285
Size: 1266 1744 3339
Fragments arranged by size:
3339 1744 1266
PciI A'CATG_T
Cuts at: 4805 4805
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 699 3047 699
Size: 2348 4001
PfoI T'CCnGG_A
Cuts at: 608 608
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3346 3346
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5488 5520 5488
Size: 32 6317
PpuMI rG'GwC_Cy
Cuts at: 2560 2560
Size: 6349
PspXI vC'TCGA_Gb
Cuts at: 2862 2862
Size: 6349
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 436 468 436
Size: 32 6317
PstI C_TGCA'G
Cuts at: 139 306 1078 1526 1724 2646 3173 3205 3394
Size: 167 772 448 198 922 527 32 189
Cuts at: 3394 4079 139
Size: 685 2409
Fragments arranged by size:
2409 922 772 685 527 448 198 189
167 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6349
PvuII CAG'CTG
Cuts at: 311 1245 1365 1579 1644 3772 4132 311
Size: 934 120 214 65 2128 360 2528
Fragments arranged by size:
2528 2128 934 360 214 120 65
RsrII CG'GwC_CG
Cuts at: 4542 4542
Size: 6349
SacI G_AGCT'C
Cuts at: 1142 3361 1142
Size: 2219 4130
SalI G'TCGA_C
Cuts at: 3384 3384
Size: 6349
SapI GCTCTTCn'nnn_
Cuts at: 4370 4580 4370
Size: 210 6139
SbfI CC_TGCA'GG
Cuts at: 3394 3394
Size: 6349
ScaI AGT'ACT
Cuts at: 408 3334 5866 408
Size: 2926 2532 891
Fragments arranged by size:
2926 2532 891
SexAI A'CCwGG_T
Cuts at: 1316 1316
Size: 6349
SfcI C'TryA_G
Cuts at: 33 135 302 1074 1522 1720 1925 2642 3169
Size: 102 167 772 448 198 205 717 527
Cuts at: 3169 3201 3390 4075 5070 5261 33
Size: 32 189 685 995 191 1121
Fragments arranged by size:
1121 995 772 717 685 527 448 205
198 191 189 167 102 32
SfoI GGC'GCC
Cuts at: 3779 4027 3779
Size: 248 6101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6349
SmaI CCC'GGG
Cuts at: 2746 3369 2746
Size: 623 5726
SmlI C'TyrA_G
Cuts at: 1222 2312 2862 3502 4417 4911 5173 1222
Size: 1090 550 640 915 494 262 2398
Fragments arranged by size:
2398 1090 915 640 550 494 262
SnaBI TAC'GTA
Cuts at: 3339 3339
Size: 6349
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6349
SphI G_CATG'C
Cuts at: 3400 3900 3400
Size: 500 5849
SspI AAT'ATT
Cuts at: 1875 1875
Size: 6349
StuI AGG'CCT
Cuts at: 3289 3289
Size: 6349
StyI C'CwwG_G
Cuts at: 2156 3275 3481 3835 2156
Size: 1119 206 354 4670
Fragments arranged by size:
4670 1119 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3069 3288 3930 4063 4522 4733 3069
Size: 219 642 133 459 211 4685
Fragments arranged by size:
4685 642 459 219 211 133
TatI w'GTAC_w
Cuts at: 406 2489 3332 5864 406
Size: 2083 843 2532 891
Fragments arranged by size:
2532 2083 891 843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1376 1408 2984 3016 5866 5898 1376
Size: 32 1576 32 2850 32 1827
Fragments arranged by size:
2850 1827 1576 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4144 4144
Size: 6349
XbaI T'CTAG_A
Cuts at: 838 1456 3378 838
Size: 618 1922 3809
Fragments arranged by size:
3809 1922 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 552 1962 3842 282
Size: 270 1410 1880 2789
Fragments arranged by size:
2789 1880 1410 270
XhoI C'TCGA_G
Cuts at: 2862 2862
Size: 6349
XmaI C'CCGG_G
Cuts at: 2744 3367 2744
Size: 623 5726
XmnI GAAnn'nnTTC
Cuts at: 391 598 1729 391
Size: 207 1131 5011
Fragments arranged by size:
5011 1131 207
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NsiI PacI PmlI PshAI PsiI PspOMI SacII
SanDI SfiI SgrAI SrfI SwaI ZraI