Restriction map for
pF1KSDA0359
(Circular) (Six-base) MAPSORT of: pF1KSDA0359.seq Check: 9589 from: 1 to: 5380
pF1KSDA0359 5380 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 234 234
Size: 5380
AccI GT'mk_AC
Cuts at: 2416 2416
Size: 5380
Acc65I G'GTAC_C
Cuts at: 2394 2394
Size: 5380
AclI AA'CG_TT
Cuts at: 5220 5220
Size: 5380
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 1753 3203 3635 4384 4652 1474
Size: 279 1450 432 749 268 2202
Fragments arranged by size:
2202 1450 749 432 279 268
AflIII A'CryG_T
Cuts at: 2730 3836 4813 2730
Size: 1106 977 3297
Fragments arranged by size:
3297 1106 977
AgeI A'CCGG_T
Cuts at: 3768 3768
Size: 5380
AleI CACnn'nnGTG
Cuts at: 643 833 643
Size: 190 5190
AlwNI CAG_nnn'CTG
Cuts at: 2683 4252 2683
Size: 1569 3811
ApaLI G'TGCA_C
Cuts at: 4150 4150
Size: 5380
ApoI r'AATT_y
Cuts at: 1195 2382 4733 4989 1195
Size: 1187 2351 256 1586
Fragments arranged by size:
2351 1586 1187 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5380
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5380
AvaI C'yCGr_G
Cuts at: 2348 2398 2348
Size: 50 5330
BamHI G'GATC_C
Cuts at: 2296 2403 2296
Size: 107 5273
BanI G'GyrC_C
Cuts at: 1516 2394 2808 3056 3091 1516
Size: 878 414 248 35 3805
Fragments arranged by size:
3805 878 414 248 35
BanII G_rGCy'C
Cuts at: 623 1853 2392 3422 623
Size: 1230 539 1030 2581
Fragments arranged by size:
2581 1230 1030 539
BbeI G_GCGC'C
Cuts at: 1520 2812 3060 1520
Size: 1292 248 3840
Fragments arranged by size:
3840 1292 248
BbsI GAAGACnn'nnnn_
Cuts at: 656 656
Size: 5380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1618 1652 1618
Size: 34 5346
BciVI GTATCCnnnnn_n'
Cuts at: 3276 4039 5380 3276
Size: 763 1341 3276
Fragments arranged by size:
3276 1341 763
BclI T'GATC_A
Cuts at: 2898 2898
Size: 5380
BfrBI ATG'CAT
Cuts at: 2230 2230
Size: 5380
BglII A'GATC_T
Cuts at: 563 858 2893 4932 563
Size: 295 2035 2039 1011
Fragments arranged by size:
2039 2035 1011 295
BlpI GC'TnA_GC
Cuts at: 2490 2490
Size: 5380
Bme1580I G_kGCm'C
Cuts at: 3003 3096 4154 3003
Size: 93 1058 4229
Fragments arranged by size:
4229 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 428 2590 2671 3006 5152 428
Size: 2162 81 335 2146 656
Fragments arranged by size:
2162 2146 656 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 607 777 3745 607
Size: 170 2968 2242
Fragments arranged by size:
2968 2242 170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 1621 2005 1408
Size: 213 384 4783
Fragments arranged by size:
4783 384 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 637 1204 1855 2554 3433 3927 4225 637
Size: 567 651 699 879 494 298 1792
Fragments arranged by size:
1792 879 699 651 567 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1675 2028 5084 1675
Size: 353 3056 1971
Fragments arranged by size:
3056 1971 353
BsaAI yAC'GTr
Cuts at: 2370 2731 2370
Size: 361 5019
BsaBI GATnn'nnATC
Cuts at: 6 2892 4931 6
Size: 2886 2039 455
Fragments arranged by size:
2886 2039 455
BsaHI Gr'CG_yC
Cuts at: 1517 2809 3057 3761 5257 1517
Size: 1292 248 704 1496 1640
Fragments arranged by size:
1640 1496 1292 704 248
BsaWI w'CCGG_w
Cuts at: 2567 3088 3768 4042 4189 4600 2567
Size: 521 680 274 147 411 3347
Fragments arranged by size:
3347 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 635 665 1266 1296 1528 1558 635
Size: 30 601 30 232 30 4457
Fragments arranged by size:
4457 601 232 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 488 589 1015 1324 1327 1330 1333 1351 1450
Size: 101 426 309 3 3 3 18 99
Cuts at: 1450 1693 2292 2295 488
Size: 243 599 3 3573
Fragments arranged by size:
3573 599 426 309 243 101 99 18
3 3 3 3
BseYI C'CCAG_C
Cuts at: 237 1226 1512 2128 2803 3561 4140 4636 237
Size: 989 286 616 675 758 579 496 981
Fragments arranged by size:
989 981 758 675 616 579 496 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 819
Size: 5380
BsiEI CG_ry'CG
Cuts at: 104 2920 2966 4176 104
Size: 2816 46 1210 1308
Fragments arranged by size:
2816 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 623 758 1853 2392 3170 3360 4154 623
Size: 135 1095 539 778 190 794 1849
Fragments arranged by size:
1849 1095 794 778 539 190 135
BsmI GAATG_Cn'
Cuts at: 327 327
Size: 5380
BsmBI CGTCTCn'nnnn_
Cuts at: 343 343
Size: 5380
Bsp1286I G_dGCh'C
Cuts at: 623 758 1853 2392 3003 3096 3170 3360 3422
Size: 135 1095 539 611 93 74 190 62
Cuts at: 3422 4154 623
Size: 732 1849
Fragments arranged by size:
1849 1095 732 611 539 190 135 93
74 62
BspEI T'CCGG_A
Cuts at: 4600 4600
Size: 5380
BspHI T'CATG_A
Cuts at: 4556 4556
Size: 5380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 234 1490 2428 2642 2944 3325 234
Size: 1256 938 214 302 381 2289
Fragments arranged by size:
2289 1256 938 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3670 3724 5210 5375 50
Size: 3620 54 1486 165 55
Fragments arranged by size:
3620 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1053 1711 2162 3290 1053
Size: 658 451 1128 3143
Fragments arranged by size:
3143 1128 658 451
BsrFI r'CCGG_y
Cuts at: 889 3376 3557 3768 889
Size: 2487 181 211 2501
Fragments arranged by size:
2501 2487 211 181
BssSI C'ACGA_G
Cuts at: 3649 4009 3649
Size: 360 5020
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2755 2191
Size: 564 4816
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5380
BstEII G'GTnAC_C
Cuts at: 457 457
Size: 5380
BstYI r'GATC_y
Cuts at: 1 563 578 858 1787 2296 2403 2870 2893
Size: 562 15 280 929 509 107 467 23
Cuts at: 2893 3228 3474 4488 4574 4586 4932 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 929 562 509 467 449 346 335
280 246 107 86 23 15 12
Bsu36I CC'TnA_GG
Cuts at: 2313 2313
Size: 5380
BtgI C'CryG_G
Cuts at: 440 453 1532 2021 440
Size: 13 1079 489 3799
Fragments arranged by size:
3799 1079 489 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3328 3509 3672 3328
Size: 181 163 5036
Fragments arranged by size:
5036 181 163
BtsI GCAGTG_nn'
Cuts at: 1296 1781 2246 2621 1296
Size: 485 465 375 4055
Fragments arranged by size:
4055 485 465 375
DraI TTT'AAA
Cuts at: 2377 2377
Size: 5380
DrdI GACnn_nn'nnGTC
Cuts at: 3084 3944 3084
Size: 860 4520
EaeI y'GGCC_r
Cuts at: 1067 2008 2963 3137 3528 3555 4902 1067
Size: 941 955 174 391 27 1347 1545
Fragments arranged by size:
1545 1347 955 941 391 174 27
EagI C'GGCC_G
Cuts at: 2963 2963
Size: 5380
EarI CTCTTCn'nnn_
Cuts at: 1094 1199 1252 1301 1322 1762 1940 2313 3401
Size: 105 53 49 21 440 178 373 1088
Cuts at: 3401 3611 1094
Size: 210 2863
Fragments arranged by size:
2863 1088 440 373 210 178 105 53
49 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3586 3898 4044 5190 3586
Size: 312 146 1146 3776
Fragments arranged by size:
3776 1146 312 146
EcoICRI GAG'CTC
Cuts at: 621 1851 2390 621
Size: 1230 539 3611
Fragments arranged by size:
3611 1230 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 1474 1621 1753 2005 3203 3635 4384 4652
Size: 66 147 132 252 1198 432 749 268
Cuts at: 4652 1408
Size: 2136
Fragments arranged by size:
2136 1198 749 432 268 252 147 132
66
EcoNI CCTnn'n_nnAGG
Cuts at: 638 638
Size: 5380
EcoO109I rG'GnC_Cy
Cuts at: 1078 1170 1479 2517 1078
Size: 92 309 1038 3941
Fragments arranged by size:
3941 1038 309 92
EcoRI G'AATT_C
Cuts at: 1195 2382 1195
Size: 1187 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1054 3397 1054
Size: 2343 3037
FspI TGC'GCA
Cuts at: 3159 3159
Size: 5380
HaeII r_GCGC'y
Cuts at: 1520 1824 2812 3060 4084 5063 1520
Size: 304 988 248 1024 979 1837
Fragments arranged by size:
1837 1024 988 979 304 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 1653 1685 2058 2090 2218 2250 3220
Size: 32 1044 32 373 32 128 32 970
Cuts at: 3220 3250 3252 3282 577
Size: 30 2 30 2675
Fragments arranged by size:
2675 1044 970 373 128 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 369 2417 369
Size: 2048 3332
HindIII A'AGCT_T
Cuts at: 1012 1765 1012
Size: 753 4627
KasI G'GCGC_C
Cuts at: 1516 2808 3056 1516
Size: 1292 248 3840
Fragments arranged by size:
3840 1292 248
KpnI G_GTAC'C
Cuts at: 2398 2398
Size: 5380
MluI A'CGCG_T
Cuts at: 4813 4813
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 381 1533 4051 4235 381
Size: 1152 2518 184 1526
Fragments arranged by size:
2518 1526 1152 184
MscI TGG'CCA
Cuts at: 1069 3139 1069
Size: 2070 3310
MslI CAynn'nnrTG
Cuts at: 643 833 1580 3367 3494 643
Size: 190 747 1787 127 2529
Fragments arranged by size:
2529 1787 747 190 127
MspA1I CmG'CkG
Cuts at: 1230 2489 2803 3163 4178 4423 1230
Size: 1259 314 360 1015 245 2187
Fragments arranged by size:
2187 1259 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3559 3559
Size: 5380
NarI GG'CG_CC
Cuts at: 1517 2809 3057 1517
Size: 1292 248 3840
Fragments arranged by size:
3840 1292 248
NcoI C'CATG_G
Cuts at: 440 1532 440
Size: 1092 4288
NgoMIV G'CCGG_C
Cuts at: 3557 3557
Size: 5380
NruI TCG'CGA
Cuts at: 3741 3741
Size: 5380
NsiI A_TGCA'T
Cuts at: 2232 2232
Size: 5380
NspI r_CATG'y
Cuts at: 703 2127 2431 2931 3840 703
Size: 1424 304 500 909 2243
Fragments arranged by size:
2243 1424 909 500 304
PciI A'CATG_T
Cuts at: 3836 3836
Size: 5380
PfoI T'CCnGG_A
Cuts at: 1199 1598 1199
Size: 399 4981
PmeI GTTT'AAAC
Cuts at: 2377 2377
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4519 4551 4519
Size: 32 5348
PpuMI rG'GwC_Cy
Cuts at: 1170 1479 1170
Size: 309 5071
PstI C_TGCA'G
Cuts at: 2204 2425 3110 2204
Size: 221 685 4474
Fragments arranged by size:
4474 685 221
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5380
PvuII CAG'CTG
Cuts at: 1230 2803 3163 1230
Size: 1573 360 3447
Fragments arranged by size:
3447 1573 360
RsrII CG'GwC_CG
Cuts at: 3573 3573
Size: 5380
SacI G_AGCT'C
Cuts at: 623 1853 2392 623
Size: 1230 539 3611
Fragments arranged by size:
3611 1230 539
SalI G'TCGA_C
Cuts at: 2415 2415
Size: 5380
SapI GCTCTTCn'nnn_
Cuts at: 1762 3401 3611 1762
Size: 1639 210 3531
Fragments arranged by size:
3531 1639 210
SbfI CC_TGCA'GG
Cuts at: 2425 2425
Size: 5380
ScaI AGT'ACT
Cuts at: 4897 4897
Size: 5380
SexAI A'CCwGG_T
Cuts at: 539 539
Size: 5380
SfcI C'TryA_G
Cuts at: 33 2200 2421 3106 4101 4292 33
Size: 2167 221 685 995 191 1121
Fragments arranged by size:
2167 1121 995 685 221 191
SfoI GGC'GCC
Cuts at: 1518 2810 3058 1518
Size: 1292 248 3840
Fragments arranged by size:
3840 1292 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5380
SmaI CCC'GGG
Cuts at: 2400 2400
Size: 5380
SmlI C'TyrA_G
Cuts at: 616 1219 1870 2533 3448 3942 4204 616
Size: 603 651 663 915 494 262 1792
Fragments arranged by size:
1792 915 663 651 603 494 262
SnaBI TAC'GTA
Cuts at: 2370 2370
Size: 5380
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5380
SphI G_CATG'C
Cuts at: 2431 2931 2431
Size: 500 4880
StuI AGG'CCT
Cuts at: 633 2274 633
Size: 1641 3739
StyI C'CwwG_G
Cuts at: 440 1038 1156 1175 1532 1685 2171 2264 2512
Size: 598 118 19 357 153 486 93 248
Cuts at: 2512 2866 440
Size: 354 2954
Fragments arranged by size:
2954 598 486 357 354 248 153 118
93 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 2961 3094 3553 3764 523
Size: 2438 133 459 211 2139
Fragments arranged by size:
2438 2139 459 211 133
TatI w'GTAC_w
Cuts at: 4895 4895
Size: 5380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 635 667 978 1010 1266 1298 4897 4929 635
Size: 32 311 32 256 32 3599 32 1086
Fragments arranged by size:
3599 1086 311 256 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 373 3175 373
Size: 2802 2578
XbaI T'CTAG_A
Cuts at: 2409 2409
Size: 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1036 1059 2873 1036
Size: 23 1814 3543
Fragments arranged by size:
3543 1814 23
XmaI C'CCGG_G
Cuts at: 2398 2398
Size: 5380
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
XmnI ZraI