Restriction map for
pF1KSDA0360
(Circular) (Six-base) MAPSORT of: pF1KSDA0360.seq Check: 7445 from: 1 to: 6158
pF1KSDA0360 6158 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 732 732
Size: 6158
AccI GT'mk_AC
Cuts at: 3196 3196
Size: 6158
Acc65I G'GTAC_C
Cuts at: 3174 3174
Size: 6158
AclI AA'CG_TT
Cuts at: 5998 5998
Size: 6158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 834 902 1189 2324 3983 4415 5162 5430
Size: 361 68 287 1135 1659 432 747 268
Cuts at: 5430 473
Size: 1201
Fragments arranged by size:
1659 1201 1135 747 432 361 287 268
68
AflIII A'CryG_T
Cuts at: 1410 3510 4614 5591 1410
Size: 2100 1104 977 1977
Fragments arranged by size:
2100 1977 1104 977
AgeI A'CCGG_T
Cuts at: 4546 4546
Size: 6158
AleI CACnn'nnGTG
Cuts at: 1313 2785 1313
Size: 1472 4686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 760 1489 760
Size: 729 5429
AlwNI CAG_nnn'CTG
Cuts at: 430 526 747 817 1908 3463 5030 430
Size: 96 221 70 1091 1555 1567 1558
Fragments arranged by size:
1567 1558 1555 1091 221 96 70
ApaI G_GGCC'C
Cuts at: 466 2061 466
Size: 1595 4563
ApaLI G'TGCA_C
Cuts at: 2150 4928 2150
Size: 2778 3380
ApoI r'AATT_y
Cuts at: 268 2196 3162 5511 5767 268
Size: 1928 966 2349 256 659
Fragments arranged by size:
2349 1928 966 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
AvaI C'yCGr_G
Cuts at: 1133 2054 2186 3178 1133
Size: 921 132 992 4113
Fragments arranged by size:
4113 992 921 132
BamHI G'GATC_C
Cuts at: 450 3183 450
Size: 2733 3425
BanI G'GyrC_C
Cuts at: 778 3174 3588 3836 3871 778
Size: 2396 414 248 35 3065
Fragments arranged by size:
3065 2396 414 248 35
BanII G_rGCy'C
Cuts at: 466 1166 1726 1868 1968 2061 3110 3172 4202
Size: 700 560 142 100 93 1049 62 1030
Cuts at: 4202 466
Size: 2422
Fragments arranged by size:
2422 1049 1030 700 560 142 100 93
62
BbeI G_GCGC'C
Cuts at: 3592 3840 3592
Size: 248 5910
BbsI GAAGACnn'nnnn_
Cuts at: 479 2621 2923 479
Size: 2142 302 3714
Fragments arranged by size:
3714 2142 302
BbvCI CC'TCA_GC
Cuts at: 1950 1998 2672 1950
Size: 48 674 5436
Fragments arranged by size:
5436 674 48
BciVI GTATCCnnnnn_n'
Cuts at: 4056 4817 6158 4056
Size: 761 1341 4056
Fragments arranged by size:
4056 1341 761
BclI T'GATC_A
Cuts at: 3678 3678
Size: 6158
BglII A'GATC_T
Cuts at: 3673 5710 3673
Size: 2037 4121
BlpI GC'TnA_GC
Cuts at: 1195 1725 2044 2806 3270 1195
Size: 530 319 762 464 4083
Fragments arranged by size:
4083 762 530 464 319
Bme1580I G_kGCm'C
Cuts at: 446 466 783 1287 1809 2061 2154 2193 3783
Size: 20 317 504 522 252 93 39 1590
Cuts at: 3783 3876 4932 446
Size: 93 1056 1672
Fragments arranged by size:
1672 1590 1056 522 504 317 252 93
93 39 20
BmrI ACTGGGnnnn_n'
Cuts at: 976 1426 3370 3451 3786 5930 976
Size: 450 1944 81 335 2144 1204
Fragments arranged by size:
2144 1944 1204 450 335 81
BmtI G_CTAG'C
Cuts at: 209 209
Size: 6158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1613 2349 2757 4525 1613
Size: 736 408 1768 3246
Fragments arranged by size:
3246 1768 736 408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 1013 1511 1616 2923 569
Size: 444 498 105 1307 3804
Fragments arranged by size:
3804 1307 498 444 105
Bpu10I CC'TnA_GC
Cuts at: 1950 1998 2321 2672 1950
Size: 48 323 351 5436
Fragments arranged by size:
5436 351 323 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2727 2974 2995 3334 4213 4705 5003 1162
Size: 1565 247 21 339 879 492 298 2317
Fragments arranged by size:
2317 1565 879 492 339 298 247 21
BsaI GGTCTCn'nnnn_
Cuts at: 5862 5862
Size: 6158
BsaAI yAC'GTr
Cuts at: 3150 3511 3150
Size: 361 5797
BsaBI GATnn'nnATC
Cuts at: 6 3672 5709 6
Size: 3666 2037 455
Fragments arranged by size:
3666 2037 455
BsaHI Gr'CG_yC
Cuts at: 3589 3837 4539 6035 3589
Size: 248 702 1496 3712
Fragments arranged by size:
3712 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2255 2397 2730 3347 3868 4546 4820 4967 5378
Size: 142 333 617 521 678 274 147 411
Cuts at: 5378 2255
Size: 3035
Fragments arranged by size:
3035 678 617 521 411 333 274 147
142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 288 318 288
Size: 30 6128
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 348 486 491 496 599 602 888 891
Size: 122 138 5 5 103 3 286 3
Cuts at: 891 894 897 1525 1528 2327 2419 2431 2434
Size: 3 3 628 3 799 92 12 3
Cuts at: 2434 2443 2566 2569 2815 2920 226
Size: 9 123 3 246 105 3464
Fragments arranged by size:
3464 799 628 286 246 138 123 122
105 103 92 12 9 5 5 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 181 526 564 1035 1802 2372 2384 2587 3583
Size: 345 38 471 767 570 12 203 996
Cuts at: 3583 4341 4918 5414 181
Size: 758 577 496 925
Fragments arranged by size:
996 925 767 758 577 570 496 471
345 203 38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1435 1634 1970 2279 584
Size: 851 199 336 309 4463
Fragments arranged by size:
4463 851 336 309 199
BsiEI CG_ry'CG
Cuts at: 104 3700 3746 4954 104
Size: 3596 46 1208 1308
Fragments arranged by size:
3596 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1445 1664 2154 3172 3950 4140 4932 1445
Size: 219 490 1018 778 190 792 2671
Fragments arranged by size:
2671 1018 792 778 490 219 190
BsmI GAATG_Cn'
Cuts at: 1084 1084
Size: 6158
Bsp1286I G_dGCh'C
Cuts at: 446 466 783 1166 1287 1445 1664 1726 1809
Size: 20 317 383 121 158 219 62 83
Cuts at: 1809 1868 1968 2061 2154 2193 3110 3172 3783
Size: 59 100 93 93 39 917 62 611
Cuts at: 3783 3876 3950 4140 4202 4932 446
Size: 93 74 190 62 730 1672
Fragments arranged by size:
1672 917 730 611 383 317 219 190
158 121 100 93 93 93 83 74
62 62 62 59 39 20
BspEI T'CCGG_A
Cuts at: 2255 5378 2255
Size: 3123 3035
BspHI T'CATG_A
Cuts at: 1664 5334 1664
Size: 3670 2488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 732 3208 3422 3724 4105 732
Size: 2476 214 302 381 2785
Fragments arranged by size:
2785 2476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2940 2979 4450 4504 5988 6153 50
Size: 2890 39 1471 54 1484 165 55
Fragments arranged by size:
2890 1484 1471 165 55 54 39
BsrDI GCAATG_nn'
Cuts at: 1341 2828 4070 1341
Size: 1487 1242 3429
Fragments arranged by size:
3429 1487 1242
BsrFI r'CCGG_y
Cuts at: 1388 1925 4156 4337 4546 1388
Size: 537 2231 181 209 3000
Fragments arranged by size:
3000 2231 537 209 181
BssSI C'ACGA_G
Cuts at: 4429 4787 4429
Size: 358 5800
BstAPI GCAn_nnn'nTGC
Cuts at: 1579 3535 1579
Size: 1956 4202
BstBI TT'CG_AA
Cuts at: 110 4519 110
Size: 4409 1749
BstEII G'GTnAC_C
Cuts at: 1944 1944
Size: 6158
BstYI r'GATC_y
Cuts at: 1 450 704 1292 2276 3183 3650 3673 4008
Size: 449 254 588 984 907 467 23 335
Cuts at: 4008 4254 5266 5352 5364 5710 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 984 907 588 467 449 449 346
335 254 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 374 392 762 1959 374
Size: 18 370 1197 4573
Fragments arranged by size:
4573 1197 370 18
BtgI C'CryG_G
Cuts at: 446 1097 1362 1625 3039 446
Size: 651 265 263 1414 3565
Fragments arranged by size:
3565 1414 651 265 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4108 4289 4452 4108
Size: 181 163 5814
Fragments arranged by size:
5814 181 163
BtsI GCAGTG_nn'
Cuts at: 651 1148 1281 1473 1679 1807 2700 3401 651
Size: 497 133 192 206 128 893 701 3408
Fragments arranged by size:
3408 893 701 497 206 192 133 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1323 1358 2097 2132 1323
Size: 35 739 35 5349
Fragments arranged by size:
5349 739 35 35
DraI TTT'AAA
Cuts at: 3157 3157
Size: 6158
DraIII CAC_nnn'GTG
Cuts at: 1579 1579
Size: 6158
DrdI GACnn_nn'nnGTC
Cuts at: 3864 4722 3864
Size: 858 5300
EaeI y'GGCC_r
Cuts at: 551 648 2076 2874 3743 3917 4308 4335 5680
Size: 97 1428 798 869 174 391 27 1345
Cuts at: 5680 551
Size: 1029
Fragments arranged by size:
1428 1345 1029 869 798 391 174 97
27
EagI C'GGCC_G
Cuts at: 3743 3743
Size: 6158
EarI CTCTTCn'nnn_
Cuts at: 354 671 828 853 915 921 2010 2396 2420
Size: 317 157 25 62 6 1089 386 24
Cuts at: 2420 2438 2528 2711 2947 4181 4391 354
Size: 18 90 183 236 1234 210 2121
Fragments arranged by size:
2121 1234 1089 386 317 236 210 183
157 90 62 25 24 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 617 4366 4676 4822 5968 617
Size: 3749 310 146 1146 807
Fragments arranged by size:
3749 1146 807 310 146
EcoICRI GAG'CTC
Cuts at: 3170 3170
Size: 6158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 569 834 902 1013 1189 1511 1616 2324
Size: 96 265 68 111 176 322 105 708
Cuts at: 2324 2923 3983 4415 5162 5430 473
Size: 599 1060 432 747 268 1201
Fragments arranged by size:
1201 1060 747 708 599 432 322 268
265 176 111 105 96 68
EcoNI CCTnn'n_nnAGG
Cuts at: 809 2815 809
Size: 2006 4152
EcoO109I rG'GnC_Cy
Cuts at: 178 434 462 540 1216 1322 1874 2058 2096
Size: 256 28 78 676 106 552 184 38
Cuts at: 2096 3297 178
Size: 1201 3039
Fragments arranged by size:
3039 1201 676 552 256 184 106 78
38 28
EcoRI G'AATT_C
Cuts at: 268 2196 3162 268
Size: 1928 966 3264
Fragments arranged by size:
3264 1928 966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 4177 960
Size: 3217 2941
FspI TGC'GCA
Cuts at: 246 3939 246
Size: 3693 2465
HaeII r_GCGC'y
Cuts at: 3592 3840 4862 5841 3592
Size: 248 1022 979 3909
Fragments arranged by size:
3909 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 2395 2427 4000 4030 4032 4062 418
Size: 32 1945 32 1573 30 2 30 2514
Fragments arranged by size:
2514 1945 1573 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3197 3197
Size: 6158
KasI G'GCGC_C
Cuts at: 3588 3836 3588
Size: 248 5910
KpnI G_GTAC'C
Cuts at: 3178 3178
Size: 6158
MluI A'CGCG_T
Cuts at: 5591 5591
Size: 6158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4829 5013 4829
Size: 184 5974
MscI TGG'CCA
Cuts at: 553 650 2078 2876 3919 553
Size: 97 1428 798 1043 2792
Fragments arranged by size:
2792 1428 1043 798 97
MslI CAynn'nnrTG
Cuts at: 1313 2087 2785 4147 4274 1313
Size: 774 698 1362 127 3197
Fragments arranged by size:
3197 1362 774 698 127
MspA1I CmG'CkG
Cuts at: 144 523 699 1062 1954 2182 2587 3269 3583
Size: 379 176 363 892 228 405 682 314
Cuts at: 3583 3943 4956 5201 144
Size: 360 1013 245 1101
Fragments arranged by size:
1101 1013 892 682 405 379 363 360
314 245 228 176
NaeI GCC'GGC
Cuts at: 4339 4339
Size: 6158
NarI GG'CG_CC
Cuts at: 3589 3837 3589
Size: 248 5910
NcoI C'CATG_G
Cuts at: 3039 3039
Size: 6158
NdeI CA'TA_TG
Cuts at: 2999 2999
Size: 6158
NgoMIV G'CCGG_C
Cuts at: 4337 4337
Size: 6158
NheI G'CTAG_C
Cuts at: 205 205
Size: 6158
NspI r_CATG'y
Cuts at: 293 1414 1770 2254 3211 3711 4618 293
Size: 1121 356 484 957 500 907 1833
Fragments arranged by size:
1833 1121 957 907 500 484 356
PasI CC'CwG_GG
Cuts at: 634 1159 1711 634
Size: 525 552 5081
Fragments arranged by size:
5081 552 525
PciI A'CATG_T
Cuts at: 1410 4614 1410
Size: 3204 2954
PflMI CCAn_nnn'nTGG
Cuts at: 430 774 966 1180 430
Size: 344 192 214 5408
Fragments arranged by size:
5408 344 214 192
PfoI T'CCnGG_A
Cuts at: 1632 1632
Size: 6158
PmeI GTTT'AAAC
Cuts at: 3157 3157
Size: 6158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 5297 5329 114
Size: 32 5151 32 943
Fragments arranged by size:
5151 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 178 434 1216 178
Size: 256 782 5120
Fragments arranged by size:
5120 782 256
PspOMI G'GGCC_C
Cuts at: 462 2057 462
Size: 1595 4563
PstI C_TGCA'G
Cuts at: 695 739 1292 1904 2246 2816 2962 3205 3890
Size: 44 553 612 342 570 146 243 685
Cuts at: 3890 695
Size: 2963
Fragments arranged by size:
2963 685 612 570 553 342 243 146
44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6158
PvuII CAG'CTG
Cuts at: 1062 1954 2182 2587 3583 3943 1062
Size: 892 228 405 996 360 3277
Fragments arranged by size:
3277 996 892 405 360 228
RsrII CG'GwC_CG
Cuts at: 4353 4353
Size: 6158
SacI G_AGCT'C
Cuts at: 3172 3172
Size: 6158
SalI G'TCGA_C
Cuts at: 3195 3195
Size: 6158
SanDI GG'GwC_CC
Cuts at: 1216 1216
Size: 6158
SapI GCTCTTCn'nnn_
Cuts at: 671 2947 4181 4391 671
Size: 2276 1234 210 2438
Fragments arranged by size:
2438 2276 1234 210
SbfI CC_TGCA'GG
Cuts at: 2816 3205 2816
Size: 389 5769
ScaI AGT'ACT
Cuts at: 5675 5675
Size: 6158
SexAI A'CCwGG_T
Cuts at: 3017 3017
Size: 6158
SfcI C'TryA_G
Cuts at: 33 691 735 1288 1900 2242 2345 2812 2958
Size: 658 44 553 612 342 103 467 146
Cuts at: 2958 3201 3886 4879 5070 33
Size: 243 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 612 553 467 342
243 191 146 103 44
SfoI GGC'GCC
Cuts at: 3590 3838 3590
Size: 248 5910
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6158
SmaI CCC'GGG
Cuts at: 2188 3180 2188
Size: 992 5166
SmlI C'TyrA_G
Cuts at: 1141 2742 2974 2989 3313 4228 4720 4982 1141
Size: 1601 232 15 324 915 492 262 2317
Fragments arranged by size:
2317 1601 915 492 324 262 232 15
SnaBI TAC'GTA
Cuts at: 3150 3150
Size: 6158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6158
SphI G_CATG'C
Cuts at: 1770 3211 3711 1770
Size: 1441 500 4217
Fragments arranged by size:
4217 1441 500
SrfI GCCC'GGGC
Cuts at: 2188 2188
Size: 6158
SspI AAT'ATT
Cuts at: 3054 3054
Size: 6158
StuI AGG'CCT
Cuts at: 943 1139 2860 2881 943
Size: 196 1721 21 4220
Fragments arranged by size:
4220 1721 196 21
StyI C'CwwG_G
Cuts at: 1220 1868 2792 2927 3039 3292 3646 1220
Size: 648 924 135 112 253 354 3732
Fragments arranged by size:
3732 924 648 354 253 135 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 766 977 1445 3741 3874 4333 4542 766
Size: 211 468 2296 133 459 209 2382
Fragments arranged by size:
2382 2296 468 459 211 209 133
TatI w'GTAC_w
Cuts at: 5673 5673
Size: 6158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1288 1320 1813 1845 5675 5707 1288
Size: 32 493 32 3830 32 1739
Fragments arranged by size:
3830 1739 493 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1453 3955 1453
Size: 2502 3656
XbaI T'CTAG_A
Cuts at: 3189 3189
Size: 6158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 882 1104 1737 2790 3653 561
Size: 321 222 633 1053 863 3066
Fragments arranged by size:
3066 1053 863 633 321 222
XmaI C'CCGG_G
Cuts at: 2186 3178 2186
Size: 992 5166
XmnI GAAnn'nnTTC
Cuts at: 2221 2221
Size: 6158
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SfiI SgrAI SwaI XhoI ZraI