Restriction map for
pF1KSDA0378
(Circular) (Six-base) MAPSORT of: pF1KSDA0378.seq Check: 9648 from: 1 to: 6010
pF1KSDA0378 6010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3046 3046
Size: 6010
Acc65I G'GTAC_C
Cuts at: 3024 3024
Size: 6010
AclI AA'CG_TT
Cuts at: 5850 5850
Size: 6010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1207 1462 1573 1741 3833 4265 5014 5282
Size: 648 255 111 168 2092 432 749 268
Cuts at: 5282 559
Size: 1287
Fragments arranged by size:
2092 1287 749 648 432 268 255 168
111
AfeI AGC'GCT
Cuts at: 1921 1921
Size: 6010
AflII C'TTAA_G
Cuts at: 694 694
Size: 6010
AflIII A'CryG_T
Cuts at: 3360 4466 5443 3360
Size: 1106 977 3927
Fragments arranged by size:
3927 1106 977
AgeI A'CCGG_T
Cuts at: 2306 4398 2306
Size: 2092 3918
AlwNI CAG_nnn'CTG
Cuts at: 1129 1867 2571 2613 3313 4882 1129
Size: 738 704 42 700 1569 2257
Fragments arranged by size:
2257 1569 738 704 700 42
ApaLI G'TGCA_C
Cuts at: 4780 4780
Size: 6010
ApoI r'AATT_y
Cuts at: 1515 1703 3012 5363 5619 1515
Size: 188 1309 2351 256 1906
Fragments arranged by size:
2351 1906 1309 256 188
AseI AT'TA_AT
Cuts at: 20 20
Size: 6010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6010
AvaI C'yCGr_G
Cuts at: 1025 3028 1025
Size: 2003 4007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 295 328 295
Size: 33 5977
BamHI G'GATC_C
Cuts at: 3033 3033
Size: 6010
BanI G'GyrC_C
Cuts at: 3024 3438 3686 3721 3024
Size: 414 248 35 5313
Fragments arranged by size:
5313 414 248 35
BanII G_rGCy'C
Cuts at: 3022 4052 3022
Size: 1030 4980
BbeI G_GCGC'C
Cuts at: 3442 3690 3442
Size: 248 5762
BbsI GAAGACnn'nnnn_
Cuts at: 976 1429 2501 2563 976
Size: 453 1072 62 4423
Fragments arranged by size:
4423 1072 453 62
BbvCI CC'TCA_GC
Cuts at: 1504 1504
Size: 6010
BciVI GTATCCnnnnn_n'
Cuts at: 3906 4669 6010 3906
Size: 763 1341 3906
Fragments arranged by size:
3906 1341 763
BclI T'GATC_A
Cuts at: 321 1434 3528 321
Size: 1113 2094 2803
Fragments arranged by size:
2803 2094 1113
BfrBI ATG'CAT
Cuts at: 2074 2074
Size: 6010
BglII A'GATC_T
Cuts at: 570 3523 5562 570
Size: 2953 2039 1018
Fragments arranged by size:
2953 2039 1018
BlpI GC'TnA_GC
Cuts at: 3120 3120
Size: 6010
Bme1580I G_kGCm'C
Cuts at: 361 3633 3726 4784 361
Size: 3272 93 1058 1587
Fragments arranged by size:
3272 1587 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 3220 3301 3636 5782 3220
Size: 81 335 2146 3448
Fragments arranged by size:
3448 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2402 4375 2402
Size: 1973 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 835 1111 1237 2193 2335 2395 2572 2689
Size: 133 276 126 956 142 60 177 117
Cuts at: 2689 2701 702
Size: 12 4011
Fragments arranged by size:
4011 956 276 177 142 133 126 117
60 12
Bpu10I CC'TnA_GC
Cuts at: 689 947 1504 689
Size: 258 557 5195
Fragments arranged by size:
5195 557 258
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 732 1072 1336 2308 2413 2779 3184 4063
Size: 2 340 264 972 105 366 405 879
Cuts at: 4063 4557 4855 730
Size: 494 298 1885
Fragments arranged by size:
1885 972 879 494 405 366 340 298
264 105 2
BsaI GGTCTCn'nnnn_
Cuts at: 200 827 5714 200
Size: 627 4887 496
Fragments arranged by size:
4887 627 496
BsaAI yAC'GTr
Cuts at: 3000 3361 3000
Size: 361 5649
BsaBI GATnn'nnATC
Cuts at: 6 2882 3522 5561 6
Size: 2876 640 2039 455
Fragments arranged by size:
2876 2039 640 455
BsaHI Gr'CG_yC
Cuts at: 2260 3439 3687 4391 5887 2260
Size: 1179 248 704 1496 2383
Fragments arranged by size:
2383 1496 1179 704 248
BsaWI w'CCGG_w
Cuts at: 617 2306 2645 2964 3197 3718 4398 4672 4819
Size: 1689 339 319 233 521 680 274 147
Cuts at: 4819 5230 617
Size: 411 1397
Fragments arranged by size:
1689 1397 680 521 411 339 319 274
233 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 392 422 392
Size: 30 5980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 188 604 838 887 919 2670 2686 2995 188
Size: 416 234 49 32 1751 16 309 3203
Fragments arranged by size:
3203 1751 416 309 234 49 32 16
BseYI C'CCAG_C
Cuts at: 581 3433 4191 4770 5266 581
Size: 2852 758 579 496 1325
Fragments arranged by size:
2852 1325 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1853 1853
Size: 6010
BsiEI CG_ry'CG
Cuts at: 104 3550 3596 4806 104
Size: 3446 46 1210 1308
Fragments arranged by size:
3446 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 884 1547 3022 3800 3990 4784 884
Size: 663 1475 778 190 794 2110
Fragments arranged by size:
2110 1475 794 778 663 190
BsmI GAATG_Cn'
Cuts at: 2550 2550
Size: 6010
Bsp1286I G_dGCh'C
Cuts at: 361 884 1547 3022 3633 3726 3800 3990 4052
Size: 523 663 1475 611 93 74 190 62
Cuts at: 4052 4784 361
Size: 732 1587
Fragments arranged by size:
1587 1475 732 663 611 523 190 93
74 62
BspEI T'CCGG_A
Cuts at: 617 2645 2964 5230 617
Size: 2028 319 2266 1397
Fragments arranged by size:
2266 2028 1397 319
BspHI T'CATG_A
Cuts at: 324 5186 324
Size: 4862 1148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 3058 3272 3574 3955 537
Size: 2521 214 302 381 2592
Fragments arranged by size:
2592 2521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 877 4300 4354 5840 6005 50
Size: 827 3423 54 1486 165 55
Fragments arranged by size:
3423 1486 827 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2732 3920 2732
Size: 1188 4822
BsrFI r'CCGG_y
Cuts at: 1696 2306 4006 4187 4398 1696
Size: 610 1700 181 211 3308
Fragments arranged by size:
3308 1700 610 211 181
BssSI C'ACGA_G
Cuts at: 4279 4639 4279
Size: 360 5650
BstAPI GCAn_nnn'nTGC
Cuts at: 292 2704 2734 3385 292
Size: 2412 30 651 2917
Fragments arranged by size:
2917 2412 651 30
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 1089 1089
Size: 6010
BstYI r'GATC_y
Cuts at: 1 175 570 652 1311 1325 2318 2675 3033
Size: 174 395 82 659 14 993 357 358
Cuts at: 3033 3500 3523 3858 4104 5118 5204 5216 5562
Size: 467 23 335 246 1014 86 12 346
Cuts at: 5562 1
Size: 449
Fragments arranged by size:
1014 993 659 467 449 395 358 357
346 335 246 174 86 82 23 14
12
BtgI C'CryG_G
Cuts at: 416 416
Size: 6010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3958 4139 4302 3958
Size: 181 163 5666
Fragments arranged by size:
5666 181 163
BtsI GCAGTG_nn'
Cuts at: 3251 3251
Size: 6010
ClaI AT'CG_AT
Cuts at: 2922 2922
Size: 6010
DraI TTT'AAA
Cuts at: 3007 3007
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 3714 4574 3714
Size: 860 5150
EaeI y'GGCC_r
Cuts at: 1694 2420 2615 2636 3593 3767 4158 4185 5532
Size: 726 195 21 957 174 391 27 1347
Cuts at: 5532 1694
Size: 2172
Fragments arranged by size:
2172 1347 957 726 391 195 174 27
21
EagI C'GGCC_G
Cuts at: 3593 3593
Size: 6010
EarI CTCTTCn'nnn_
Cuts at: 716 1370 1673 1958 4031 4241 716
Size: 654 303 285 2073 210 2485
Fragments arranged by size:
2485 2073 654 303 285 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1131 4216 4528 4674 5820 1131
Size: 3085 312 146 1146 1321
Fragments arranged by size:
3085 1321 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3020 3020
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 702 835 1111 1207 1237 1462 1573 1741
Size: 143 133 276 96 30 225 111 168
Cuts at: 1741 2193 2335 2395 2572 2689 2701 3833 4265
Size: 452 142 60 177 117 12 1132 432
Cuts at: 4265 5014 5282 559
Size: 749 268 1287
Fragments arranged by size:
1287 1132 749 452 432 276 268 225
177 168 143 142 133 117 111 96
60 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2326 2326
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 528 804 1668 2957 3147 528
Size: 276 864 1289 190 3391
Fragments arranged by size:
3391 1289 864 276 190
EcoRI G'AATT_C
Cuts at: 3012 3012
Size: 6010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1573 1804 2719 4027 1573
Size: 231 915 1308 3556
Fragments arranged by size:
3556 1308 915 231
FspI TGC'GCA
Cuts at: 2485 3789 2485
Size: 1304 4706
HaeII r_GCGC'y
Cuts at: 1923 3442 3690 4714 5693 1923
Size: 1519 248 1024 979 2240
Fragments arranged by size:
2240 1519 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 392 424 1117 1149 1181 1233 1265 1957 1989
Size: 32 693 32 32 52 32 692 32
Cuts at: 1989 2029 2061 3850 3880 3882 3912 392
Size: 40 32 1789 30 2 30 2490
Fragments arranged by size:
2490 1789 693 692 52 40 32 32
32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 794 3047 794
Size: 2253 3757
HindIII A'AGCT_T
Cuts at: 1492 1492
Size: 6010
KasI G'GCGC_C
Cuts at: 3438 3686 3438
Size: 248 5762
KpnI G_GTAC'C
Cuts at: 3028 3028
Size: 6010
MluI A'CGCG_T
Cuts at: 5443 5443
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1448 2426 4681 4865 1448
Size: 978 2255 184 2593
Fragments arranged by size:
2593 2255 978 184
MscI TGG'CCA
Cuts at: 2422 2638 3769 2422
Size: 216 1131 4663
Fragments arranged by size:
4663 1131 216
MslI CAynn'nnrTG
Cuts at: 415 461 1267 1540 3997 4124 415
Size: 46 806 273 2457 127 2301
Fragments arranged by size:
2457 2301 806 273 127 46
MspA1I CmG'CkG
Cuts at: 585 1440 2667 3119 3433 3793 4808 5053 585
Size: 855 1227 452 314 360 1015 245 1542
Fragments arranged by size:
1542 1227 1015 855 452 360 314 245
NaeI GCC'GGC
Cuts at: 4189 4189
Size: 6010
NarI GG'CG_CC
Cuts at: 3439 3687 3439
Size: 248 5762
NcoI C'CATG_G
Cuts at: 416 416
Size: 6010
NdeI CA'TA_TG
Cuts at: 2396 2989 2396
Size: 593 5417
NgoMIV G'CCGG_C
Cuts at: 4187 4187
Size: 6010
NruI TCG'CGA
Cuts at: 4371 4371
Size: 6010
NsiI A_TGCA'T
Cuts at: 2076 2076
Size: 6010
NspI r_CATG'y
Cuts at: 2074 3061 3561 4470 2074
Size: 987 500 909 3614
Fragments arranged by size:
3614 987 909 500
PciI A'CATG_T
Cuts at: 4466 4466
Size: 6010
PfoI T'CCnGG_A
Cuts at: 2209 2678 2209
Size: 469 5541
PmeI GTTT'AAAC
Cuts at: 3007 3007
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5149 5181 5149
Size: 32 5978
PpuMI rG'GwC_Cy
Cuts at: 528 1668 528
Size: 1140 4870
PstI C_TGCA'G
Cuts at: 599 3055 3740 599
Size: 2456 685 2869
Fragments arranged by size:
2869 2456 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6010
PvuII CAG'CTG
Cuts at: 585 1440 2667 3433 3793 585
Size: 855 1227 766 360 2802
Fragments arranged by size:
2802 1227 855 766 360
RsrII CG'GwC_CG
Cuts at: 4203 4203
Size: 6010
SacI G_AGCT'C
Cuts at: 3022 3022
Size: 6010
SalI G'TCGA_C
Cuts at: 3045 3045
Size: 6010
SapI GCTCTTCn'nnn_
Cuts at: 4031 4241 4031
Size: 210 5800
SbfI CC_TGCA'GG
Cuts at: 3055 3055
Size: 6010
ScaI AGT'ACT
Cuts at: 5527 5527
Size: 6010
SexAI A'CCwGG_T
Cuts at: 524 524
Size: 6010
SfcI C'TryA_G
Cuts at: 33 595 787 1663 3051 3736 4731 4922 33
Size: 562 192 876 1388 685 995 191 1121
Fragments arranged by size:
1388 1121 995 876 685 562 192 191
SfoI GGC'GCC
Cuts at: 3440 3688 3440
Size: 248 5762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6010
SmaI CCC'GGG
Cuts at: 3030 3030
Size: 6010
SmlI C'TyrA_G
Cuts at: 694 711 745 1051 1315 2323 2428 2794 3163
Size: 17 34 306 264 1008 105 366 369
Cuts at: 3163 4078 4572 4834 694
Size: 915 494 262 1870
Fragments arranged by size:
1870 1008 915 494 369 366 306 264
262 105 34 17
SnaBI TAC'GTA
Cuts at: 3000 3000
Size: 6010
SpeI A'CTAG_T
Cuts at: 67 2814 67
Size: 2747 3263
SphI G_CATG'C
Cuts at: 3061 3561 3061
Size: 500 5510
SspI AAT'ATT
Cuts at: 435 2196 435
Size: 1761 4249
StyI C'CwwG_G
Cuts at: 190 416 3142 3496 190
Size: 226 2726 354 2704
Fragments arranged by size:
2726 2704 354 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 916 2825 3591 3724 4183 4394 916
Size: 1909 766 133 459 211 2532
Fragments arranged by size:
2532 1909 766 459 211 133
TatI w'GTAC_w
Cuts at: 2817 5525 2817
Size: 2708 3302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5527 5559 5527
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 255 3805 255
Size: 3550 2460
XbaI T'CTAG_A
Cuts at: 2124 3039 2124
Size: 915 5095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 859 2568 2926 3503 859
Size: 1709 358 577 3366
Fragments arranged by size:
3366 1709 577 358
XmaI C'CCGG_G
Cuts at: 3028 3028
Size: 6010
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BcgI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NheI NotI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI StuI SwaI XhoI XmnI ZraI