Restriction map for
pF1KSDA0379
(Circular) (Six-base) MAPSORT of: pF1KSDA0379.seq Check: 4601 from: 1 to: 6298
pF1KSDA0379 6298 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1350 3334 1350
Size: 1984 4314
Acc65I G'GTAC_C
Cuts at: 504 3312 504
Size: 2808 3490
AclI AA'CG_TT
Cuts at: 6138 6138
Size: 6298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 737 1289 1672 4121 4553 5302 5570 515
Size: 222 552 383 2449 432 749 268 1243
Fragments arranged by size:
2449 1243 749 552 432 383 268 222
AflII C'TTAA_G
Cuts at: 1266 1266
Size: 6298
AflIII A'CryG_T
Cuts at: 2434 3648 4754 5731 2434
Size: 1214 1106 977 3001
Fragments arranged by size:
3001 1214 1106 977
AgeI A'CCGG_T
Cuts at: 4686 4686
Size: 6298
AleI CACnn'nnGTG
Cuts at: 2321 3247 2321
Size: 926 5372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 977 1475 1564 2939 977
Size: 498 89 1375 4336
Fragments arranged by size:
4336 1375 498 89
AlwNI CAG_nnn'CTG
Cuts at: 1485 2649 3601 5170 1485
Size: 1164 952 1569 2613
Fragments arranged by size:
2613 1569 1164 952
ApaLI G'TGCA_C
Cuts at: 5068 5068
Size: 6298
ApoI r'AATT_y
Cuts at: 1374 1432 3300 5651 5907 1374
Size: 58 1868 2351 256 1765
Fragments arranged by size:
2351 1868 1765 256 58
AseI AT'TA_AT
Cuts at: 20 623 1934 2032 20
Size: 603 1311 98 4286
Fragments arranged by size:
4286 1311 603 98
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6298
AvaI C'yCGr_G
Cuts at: 431 3316 431
Size: 2885 3413
BamHI G'GATC_C
Cuts at: 1622 3321 1622
Size: 1699 4599
BanI G'GyrC_C
Cuts at: 504 613 2560 3312 3726 3974 4009 504
Size: 109 1947 752 414 248 35 2793
Fragments arranged by size:
2793 1947 752 414 248 109 35
BanII G_rGCy'C
Cuts at: 2337 3260 3310 4340 2337
Size: 923 50 1030 4295
Fragments arranged by size:
4295 1030 923 50
BbeI G_GCGC'C
Cuts at: 3730 3978 3730
Size: 248 6050
BbsI GAAGACnn'nnnn_
Cuts at: 1678 1678
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2515 2549 2515
Size: 34 6264
BciVI GTATCCnnnnn_n'
Cuts at: 1633 4194 4957 6298 1633
Size: 2561 763 1341 1633
Fragments arranged by size:
2561 1633 1341 763
BclI T'GATC_A
Cuts at: 779 1187 3816 779
Size: 408 2629 3261
Fragments arranged by size:
3261 2629 408
BfrBI ATG'CAT
Cuts at: 2236 2236
Size: 6298
BglII A'GATC_T
Cuts at: 804 1441 1848 1887 3811 5850 804
Size: 637 407 39 1924 2039 1252
Fragments arranged by size:
2039 1924 1252 637 407 39
BlpI GC'TnA_GC
Cuts at: 2653 3408 2653
Size: 755 5543
Bme1580I G_kGCm'C
Cuts at: 2120 3921 4014 5072 2120
Size: 1801 93 1058 3346
Fragments arranged by size:
3346 1801 1058 93
BmgBI CAC'GTC
Cuts at: 3249 3249
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 2418 3508 3589 3924 6070 2418
Size: 1090 81 335 2146 2646
Fragments arranged by size:
2646 2146 1090 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4663 4663
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1388 335
Size: 1053 5245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2651 3472 4351 4845 5143 2651
Size: 821 879 494 298 3806
Fragments arranged by size:
3806 879 821 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3271 6002 3271
Size: 2731 3567
BsaAI yAC'GTr
Cuts at: 3288 3649 3288
Size: 361 5937
BsaBI GATnn'nnATC
Cuts at: 6 1613 2366 3810 5849 6
Size: 1607 753 1444 2039 455
Fragments arranged by size:
2039 1607 1444 753 455
BsaHI Gr'CG_yC
Cuts at: 2089 2287 3727 3975 4679 6175 2089
Size: 198 1440 248 704 1496 2212
Fragments arranged by size:
2212 1496 1440 704 248 198
BsaWI w'CCGG_w
Cuts at: 3485 4006 4686 4960 5107 5518 3485
Size: 521 680 274 147 411 4265
Fragments arranged by size:
4265 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 308 338 704 734 1499 1529 2144 2174 2963
Size: 30 366 30 765 30 615 30 789
Cuts at: 2963 2993 308
Size: 30 3613
Fragments arranged by size:
3613 789 765 615 366 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1700 2591 1700
Size: 891 5407
BseYI C'CCAG_C
Cuts at: 3009 3721 4479 5058 5554 3009
Size: 712 758 579 496 3753
Fragments arranged by size:
3753 758 712 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1435 1524 1534 2786 1430
Size: 5 89 10 1252 4942
Fragments arranged by size:
4942 1252 89 10 5
BsiEI CG_ry'CG
Cuts at: 104 3838 3884 5094 104
Size: 3734 46 1210 1308
Fragments arranged by size:
3734 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 204 3027 3260 3310 4088 4278 5072 204
Size: 2823 233 50 778 190 794 1430
Fragments arranged by size:
2823 1430 794 778 233 190 50
BsmI GAATG_Cn'
Cuts at: 411 2023 2061 411
Size: 1612 38 4648
Fragments arranged by size:
4648 1612 38
Bsp1286I G_dGCh'C
Cuts at: 204 2120 2337 3027 3260 3310 3921 4014 4088
Size: 1916 217 690 233 50 611 93 74
Cuts at: 4088 4278 4340 5072 204
Size: 190 62 732 1430
Fragments arranged by size:
1916 1430 732 690 611 233 217 190
93 74 62 50
BspEI T'CCGG_A
Cuts at: 5518 5518
Size: 6298
BspHI T'CATG_A
Cuts at: 2829 3176 5474 2829
Size: 347 2298 3653
Fragments arranged by size:
3653 2298 347
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3346 3560 3862 4243 3346
Size: 214 302 381 5401
Fragments arranged by size:
5401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4588 4642 6128 6293 50
Size: 4538 54 1486 165 55
Fragments arranged by size:
4538 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 256 1014 2137 2497 4208 256
Size: 758 1123 360 1711 2346
Fragments arranged by size:
2346 1711 1123 758 360
BsrFI r'CCGG_y
Cuts at: 4294 4475 4686 4294
Size: 181 211 5906
Fragments arranged by size:
5906 211 181
BssSI C'ACGA_G
Cuts at: 2428 4567 4927 2428
Size: 2139 360 3799
Fragments arranged by size:
3799 2139 360
BstAPI GCAn_nnn'nTGC
Cuts at: 3673 3673
Size: 6298
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1672 1807 1672
Size: 135 6163
BstYI r'GATC_y
Cuts at: 1 183 804 1441 1622 1848 1887 3321 3788
Size: 182 621 637 181 226 39 1434 467
Cuts at: 3788 3811 4146 4392 5406 5492 5504 5850 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1434 1014 637 621 467 449 346 335
246 226 182 181 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 1734 1734
Size: 6298
BtgI C'CryG_G
Cuts at: 125 1071 125
Size: 946 5352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4246 4427 4590 4246
Size: 181 163 5954
Fragments arranged by size:
5954 181 163
BtsI GCAGTG_nn'
Cuts at: 1217 1447 2066 2763 3539 1217
Size: 230 619 697 776 3976
Fragments arranged by size:
3976 776 697 619 230
DraI TTT'AAA
Cuts at: 3295 3295
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 4002 4862 4002
Size: 860 5438
EaeI y'GGCC_r
Cuts at: 1173 3065 3881 4055 4446 4473 5820 1173
Size: 1892 816 174 391 27 1347 1651
Fragments arranged by size:
1892 1651 1347 816 391 174 27
EagI C'GGCC_G
Cuts at: 3881 3881
Size: 6298
EarI CTCTTCn'nnn_
Cuts at: 515 758 857 1024 1969 4319 4529 515
Size: 243 99 167 945 2350 210 2284
Fragments arranged by size:
2350 2284 945 243 210 167 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4504 4816 4962 6108 4504
Size: 312 146 1146 4694
Fragments arranged by size:
4694 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3258 3308 3258
Size: 50 6248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 515 737 1289 1388 1672 4121 4553 5302
Size: 180 222 552 99 284 2449 432 749
Cuts at: 5302 5570 335
Size: 268 1063
Fragments arranged by size:
2449 1063 749 552 432 284 268 222
180 99
EcoO109I rG'GnC_Cy
Cuts at: 3435 3435
Size: 6298
EcoRI G'AATT_C
Cuts at: 3300 3300
Size: 6298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3113 4315 3113
Size: 1202 5096
FspI TGC'GCA
Cuts at: 4077 4077
Size: 6298
HaeII r_GCGC'y
Cuts at: 3730 3978 5002 5981 3730
Size: 248 1024 979 4047
Fragments arranged by size:
4047 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 724 756 1103 1135 1179 1211 1934
Size: 32 553 32 347 32 44 32 723
Cuts at: 1934 1966 4138 4168 4170 4200 139
Size: 32 2172 30 2 30 2237
Fragments arranged by size:
2237 2172 723 553 347 44 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 228 350 3335 228
Size: 122 2985 3191
Fragments arranged by size:
3191 2985 122
HindIII A'AGCT_T
Cuts at: 497 3164 497
Size: 2667 3631
HpaI GTT'AAC
Cuts at: 228 228
Size: 6298
KasI G'GCGC_C
Cuts at: 3726 3974 3726
Size: 248 6050
KpnI G_GTAC'C
Cuts at: 508 3316 508
Size: 2808 3490
MluI A'CGCG_T
Cuts at: 5731 5731
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4969 5153 4969
Size: 184 6114
MscI TGG'CCA
Cuts at: 1175 3067 4057 1175
Size: 1892 990 3416
Fragments arranged by size:
3416 1892 990
MslI CAynn'nnrTG
Cuts at: 2321 3073 3247 4285 4412 2321
Size: 752 174 1038 127 4207
Fragments arranged by size:
4207 1038 752 174 127
MspA1I CmG'CkG
Cuts at: 1360 1366 1663 1692 2646 3407 3721 4081 5096
Size: 6 297 29 954 761 314 360 1015
Cuts at: 5096 5341 1360
Size: 245 2317
Fragments arranged by size:
2317 1015 954 761 360 314 297 245
29 6
NaeI GCC'GGC
Cuts at: 4477 4477
Size: 6298
NarI GG'CG_CC
Cuts at: 3727 3975 3727
Size: 248 6050
NcoI C'CATG_G
Cuts at: 125 125
Size: 6298
NgoMIV G'CCGG_C
Cuts at: 4475 4475
Size: 6298
NruI TCG'CGA
Cuts at: 4659 4659
Size: 6298
NsiI A_TGCA'T
Cuts at: 2238 2238
Size: 6298
NspI r_CATG'y
Cuts at: 728 1357 1749 2438 2920 3349 3849 4758 728
Size: 629 392 689 482 429 500 909 2268
Fragments arranged by size:
2268 909 689 629 500 482 429 392
PciI A'CATG_T
Cuts at: 2434 4754 2434
Size: 2320 3978
PmeI GTTT'AAAC
Cuts at: 3295 3295
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5437 5469 5437
Size: 32 6266
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2762 2794 2762
Size: 32 6266
PstI C_TGCA'G
Cuts at: 420 1291 1365 2718 3343 4028 420
Size: 871 74 1353 625 685 2690
Fragments arranged by size:
2690 1353 871 685 625 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6298
PvuII CAG'CTG
Cuts at: 1360 1366 1663 1692 2646 3721 4081 1360
Size: 6 297 29 954 1075 360 3577
Fragments arranged by size:
3577 1075 954 360 297 29 6
RsrII CG'GwC_CG
Cuts at: 4491 4491
Size: 6298
SacI G_AGCT'C
Cuts at: 3260 3310 3260
Size: 50 6248
SalI G'TCGA_C
Cuts at: 3333 3333
Size: 6298
SapI GCTCTTCn'nnn_
Cuts at: 4319 4529 4319
Size: 210 6088
SbfI CC_TGCA'GG
Cuts at: 3343 3343
Size: 6298
ScaI AGT'ACT
Cuts at: 3235 5815 3235
Size: 2580 3718
SfcI C'TryA_G
Cuts at: 33 416 1287 1361 2687 2714 3339 4024 5019
Size: 383 871 74 1326 27 625 685 995
Cuts at: 5019 5210 33
Size: 191 1121
Fragments arranged by size:
1326 1121 995 871 685 625 383 191
74 27
SfoI GGC'GCC
Cuts at: 3728 3976 3728
Size: 248 6050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6298
SmaI CCC'GGG
Cuts at: 3318 3318
Size: 6298
SmlI C'TyrA_G
Cuts at: 431 1266 2666 3451 4366 4860 5122 431
Size: 835 1400 785 915 494 262 1607
Fragments arranged by size:
1607 1400 915 835 785 494 262
SnaBI TAC'GTA
Cuts at: 3288 3288
Size: 6298
SpeI A'CTAG_T
Cuts at: 67 2836 67
Size: 2769 3529
SphI G_CATG'C
Cuts at: 3349 3849 3349
Size: 500 5798
SspI AAT'ATT
Cuts at: 209 621 209
Size: 412 5886
StyI C'CwwG_G
Cuts at: 125 276 3430 3784 125
Size: 151 3154 354 2639
Fragments arranged by size:
3154 2639 354 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3879 4012 4471 4682 3879
Size: 133 459 211 5495
Fragments arranged by size:
5495 459 211 133
TatI w'GTAC_w
Cuts at: 1926 3233 5813 1926
Size: 1307 2580 2411
Fragments arranged by size:
2580 2411 1307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 1499 1531 2963 2995 5815 5847 704
Size: 32 763 32 1432 32 2820 32 1155
Fragments arranged by size:
2820 1432 1155 763 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4093 4093
Size: 6298
XbaI T'CTAG_A
Cuts at: 607 801 1713 3327 607
Size: 194 912 1614 3578
Fragments arranged by size:
3578 1614 912 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3036 3791 3036
Size: 755 5543
XhoI C'TCGA_G
Cuts at: 431 431
Size: 6298
XmaI C'CCGG_G
Cuts at: 3316 3316
Size: 6298
XmnI GAAnn'nnTTC
Cuts at: 392 392
Size: 6298
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PciI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI
ZraI