Restriction map for
pF1KSDA0380
(Circular) (Six-base) MAPSORT of: pF1KSDA0380.seq Check: 5869 from: 1 to: 7705
pF1KSDA0380 7705 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 2381
Size: 7705
AatII G_ACGT'C
Cuts at: 3316 3316
Size: 7705
AccI GT'mk_AC
Cuts at: 4741 4741
Size: 7705
Acc65I G'GTAC_C
Cuts at: 4719 4719
Size: 7705
AclI AA'CG_TT
Cuts at: 7545 7545
Size: 7705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 2381 3160 3174 4070 4190 5528 5960 6709
Size: 1285 779 14 896 120 1338 432 749
Cuts at: 6709 6977 1096
Size: 268 1824
Fragments arranged by size:
1824 1338 1285 896 779 749 432 268
120 14
AflIII A'CryG_T
Cuts at: 3479 5055 6161 7138 3479
Size: 1576 1106 977 4046
Fragments arranged by size:
4046 1576 1106 977
AgeI A'CCGG_T
Cuts at: 958 6093 958
Size: 5135 2570
AhdI GACnn_n'nnGTC
Cuts at: 248 248
Size: 7705
AleI CACnn'nnGTG
Cuts at: 412 562 4513 412
Size: 150 3951 3604
Fragments arranged by size:
3951 3604 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1505 1877 2627 1505
Size: 372 750 6583
Fragments arranged by size:
6583 750 372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 7673
AlwNI CAG_nnn'CTG
Cuts at: 865 1065 1873 3561 4159 5008 6577 865
Size: 200 808 1688 598 849 1569 1993
Fragments arranged by size:
1993 1688 1569 849 808 598 200
ApaI G_GGCC'C
Cuts at: 2853 3283 3445 4622 2853
Size: 430 162 1177 5936
Fragments arranged by size:
5936 1177 430 162
ApaLI G'TGCA_C
Cuts at: 6475 6475
Size: 7705
ApoI r'AATT_y
Cuts at: 4005 4707 7058 7314 4005
Size: 702 2351 256 4396
Fragments arranged by size:
4396 2351 702 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7705
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7705
AvaI C'yCGr_G
Cuts at: 1023 2438 2588 2852 3422 3814 4723 1023
Size: 1415 150 264 570 392 909 4005
Fragments arranged by size:
4005 1415 909 570 392 264 150
BamHI G'GATC_C
Cuts at: 636 1577 4728 636
Size: 941 3151 3613
Fragments arranged by size:
3613 3151 941
BanI G'GyrC_C
Cuts at: 406 460 2821 3374 4260 4504 4719 5133 5381
Size: 54 2361 553 886 244 215 414 248
Cuts at: 5381 5416 406
Size: 35 2695
Fragments arranged by size:
2695 2361 886 553 414 248 244 215
54 35
BanII G_rGCy'C
Cuts at: 490 521 549 937 2853 3169 3283 3344 3445
Size: 31 28 388 1916 316 114 61 101
Cuts at: 3445 3716 3815 4273 4583 4622 4717 5747 490
Size: 271 99 458 310 39 95 1030 2448
Fragments arranged by size:
2448 1916 1030 458 388 316 310 271
114 101 99 95 61 39 31 28
BbeI G_GCGC'C
Cuts at: 464 5137 5385 464
Size: 4673 248 2784
Fragments arranged by size:
4673 2784 248
BbsI GAAGACnn'nnnn_
Cuts at: 164 1019 1529 1576 3965 4661 164
Size: 855 510 47 2389 696 3208
Fragments arranged by size:
3208 2389 855 696 510 47
BbvCI CC'TCA_GC
Cuts at: 682 4103 4277 4313 4319 682
Size: 3421 174 36 6 4068
Fragments arranged by size:
4068 3421 174 36 6
BciVI GTATCCnnnnn_n'
Cuts at: 5601 6364 7705 5601
Size: 763 1341 5601
Fragments arranged by size:
5601 1341 763
BclI T'GATC_A
Cuts at: 449 896 5223 449
Size: 447 4327 2931
Fragments arranged by size:
4327 2931 447
BglI GCCn_nnn'nGGC
Cuts at: 364 910 1973 364
Size: 546 1063 6096
Fragments arranged by size:
6096 1063 546
BglII A'GATC_T
Cuts at: 3290 5218 7257 3290
Size: 1928 2039 3738
Fragments arranged by size:
3738 2039 1928
BlpI GC'TnA_GC
Cuts at: 4815 4815
Size: 7705
Bme1580I G_kGCm'C
Cuts at: 2018 2853 3283 3445 4622 5328 5421 6479 2018
Size: 835 430 162 1177 706 93 1058 3244
Fragments arranged by size:
3244 1177 1058 835 706 430 162 93
BmgBI CAC'GTC
Cuts at: 560 3480 4389 560
Size: 2920 909 3876
Fragments arranged by size:
3876 2920 909
BmrI ACTGGGnnnn_n'
Cuts at: 1130 1408 1617 2168 3533 3616 4624 4915 4996
Size: 278 209 551 1365 83 1008 291 81
Cuts at: 4996 5331 7477 1130
Size: 335 2146 1358
Fragments arranged by size:
2146 1365 1358 1008 551 335 291 278
209 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2732 4292 4489 4653 6070 2614
Size: 118 1560 197 164 1417 4249
Fragments arranged by size:
4249 1560 1417 197 164 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 505 1087 1252 1939 2079 2266 2584 2812
Size: 137 582 165 687 140 187 318 228
Cuts at: 2812 2980 3066 3112 3536 3955 4010 4581 368
Size: 168 86 46 424 419 55 571 3492
Fragments arranged by size:
3492 687 582 571 424 419 318 228
187 168 165 140 137 86 55 46
Bpu10I CC'TnA_GC
Cuts at: 682 3575 4103 4277 4313 4319 4574 682
Size: 2893 528 174 36 6 255 3813
Fragments arranged by size:
3813 2893 528 255 174 36 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 1954 2137 2860 3187 4224 4411 4426 4459
Size: 632 183 723 327 1037 187 15 33
Cuts at: 4459 4879 5758 6252 6550 1322
Size: 420 879 494 298 2477
Fragments arranged by size:
2477 1037 879 723 632 494 420 327
298 187 183 33 15
BsaI GGTCTCn'nnnn_
Cuts at: 256 735 2000 2117 2735 3973 7409 256
Size: 479 1265 117 618 1238 3436 552
Fragments arranged by size:
3436 1265 1238 618 552 479 117
BsaAI yAC'GTr
Cuts at: 3081 4695 5056 3081
Size: 1614 361 5730
Fragments arranged by size:
5730 1614 361
BsaBI GATnn'nnATC
Cuts at: 6 5217 7256 6
Size: 5211 2039 455
Fragments arranged by size:
5211 2039 455
BsaHI Gr'CG_yC
Cuts at: 461 3313 4350 4643 5134 5382 6086 7582 461
Size: 2852 1037 293 491 248 704 1496 584
Fragments arranged by size:
2852 1496 1037 704 584 491 293 248
BsaWI w'CCGG_w
Cuts at: 386 958 1894 4892 5413 6093 6367 6514 6925
Size: 572 936 2998 521 680 274 147 411
Cuts at: 6925 386
Size: 1166
Fragments arranged by size:
2998 1166 936 680 572 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 464 494 3862 3892 464
Size: 30 3368 30 4277
Fragments arranged by size:
4277 3368 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 575 2458 3159 3520 4355 575
Size: 1883 701 361 835 3925
Fragments arranged by size:
3925 1883 835 701 361
BseYI C'CCAG_C
Cuts at: 537 914 1404 1573 2302 2312 3276 3434 3451
Size: 377 490 169 729 10 964 158 17
Cuts at: 3451 3530 3920 4354 5128 5886 6465 6961 537
Size: 79 390 434 774 758 579 496 1281
Fragments arranged by size:
1281 964 774 758 729 579 496 490
434 390 377 169 158 79 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 352 374 1181 2053 2172 2597 3249 3611
Size: 179 22 807 872 119 425 652 362
Cuts at: 3611 4115 173
Size: 504 3763
Fragments arranged by size:
3763 872 807 652 504 425 362 179
119 22
BsiEI CG_ry'CG
Cuts at: 104 387 1895 5245 5291 6501 104
Size: 283 1508 3350 46 1210 1308
Fragments arranged by size:
3350 1508 1308 1210 283 46
BsiHKAI G_wGCw'C
Cuts at: 549 3203 3221 3344 4717 5495 5685 6479 549
Size: 2654 18 123 1373 778 190 794 1775
Fragments arranged by size:
2654 1775 1373 794 778 190 123 18
BsmI GAATG_Cn'
Cuts at: 3369 3369
Size: 7705
Bsp1286I G_dGCh'C
Cuts at: 490 521 549 937 2018 2853 3169 3203 3221
Size: 31 28 388 1081 835 316 34 18
Cuts at: 3221 3283 3344 3445 3716 3815 4273 4583 4622
Size: 62 61 101 271 99 458 310 39
Cuts at: 4622 4717 5328 5421 5495 5685 5747 6479 490
Size: 95 611 93 74 190 62 732 1716
Fragments arranged by size:
1716 1081 835 732 611 458 388 316
310 271 190 101 99 95 93 74
62 62 61 39 34 31 28 18
BspEI T'CCGG_A
Cuts at: 6925 6925
Size: 7705
BspHI T'CATG_A
Cuts at: 6881 6881
Size: 7705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1439 1922 2381 2477 3755 4753 4967 5269 5650
Size: 483 459 96 1278 998 214 302 381
Cuts at: 5650 1439
Size: 3494
Fragments arranged by size:
3494 1278 998 483 459 381 302 214
96
BsrBI CCG'CTC
Cuts at: 50 1958 2021 3227 3727 4361 5995 6049 7535
Size: 1908 63 1206 500 634 1634 54 1486
Cuts at: 7535 7700 50
Size: 165 55
Fragments arranged by size:
1908 1634 1486 1206 634 500 165 63
55 54
BsrDI GCAATG_nn'
Cuts at: 1821 5615 1821
Size: 3794 3911
BsrFI r'CCGG_y
Cuts at: 367 755 958 2327 5701 5882 6093 367
Size: 388 203 1369 3374 181 211 1979
Fragments arranged by size:
3374 1979 1369 388 211 203 181
BssSI C'ACGA_G
Cuts at: 5974 6334 5974
Size: 360 7345
BstAPI GCAn_nnn'nTGC
Cuts at: 339 5080 339
Size: 4741 2964
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7705
BstEII G'GTnAC_C
Cuts at: 137 414 961 137
Size: 277 547 6881
Fragments arranged by size:
6881 547 277
BstXI CCAn_nnnn'nTGG
Cuts at: 2958 3636 3681 4515 2958
Size: 678 45 834 6148
Fragments arranged by size:
6148 834 678 45
BstYI r'GATC_y
Cuts at: 1 636 665 1062 1259 1358 1370 1577 3003
Size: 635 29 397 197 99 12 207 1426
Cuts at: 3003 3290 4728 5195 5218 5553 5799 6813 6899
Size: 287 1438 467 23 335 246 1014 86
Cuts at: 6899 6911 7257 1
Size: 12 346 449
Fragments arranged by size:
1438 1426 1014 635 467 449 397 346
335 287 246 207 197 99 86 29
23 12 12
Bsu36I CC'TnA_GG
Cuts at: 670 1907 3503 4381 4628 670
Size: 1237 1596 878 247 3747
Fragments arranged by size:
3747 1596 1237 878 247
BtgI C'CryG_G
Cuts at: 410 503 1317 1538 3486 3788 410
Size: 93 814 221 1948 302 4327
Fragments arranged by size:
4327 1948 814 302 221 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 202 5653 5834 5997 202
Size: 5451 181 163 1910
Fragments arranged by size:
5451 1910 181 163
BtsI GCAGTG_nn'
Cuts at: 1141 2034 3594 4946 1141
Size: 893 1560 1352 3900
Fragments arranged by size:
3900 1560 1352 893
DraI TTT'AAA
Cuts at: 4702 4702
Size: 7705
DrdI GACnn_nn'nnGTC
Cuts at: 5409 6269 5409
Size: 860 6845
EaeI y'GGCC_r
Cuts at: 902 1084 3233 3946 4146 5288 5462 5853 5880
Size: 182 2149 713 200 1142 174 391 27
Cuts at: 5880 7227 902
Size: 1347 1380
Fragments arranged by size:
2149 1380 1347 1142 713 391 200 182
174 27
EagI C'GGCC_G
Cuts at: 5288 5288
Size: 7705
EarI CTCTTCn'nnn_
Cuts at: 495 1669 1767 1976 2464 2695 3344 3593 5726
Size: 1174 98 209 488 231 649 249 2133
Cuts at: 5726 5936 495
Size: 210 2264
Fragments arranged by size:
2264 2133 1174 649 488 249 231 210
209 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1144 4196 5911 6223 6369 7515 1144
Size: 3052 1715 312 146 1146 1334
Fragments arranged by size:
3052 1715 1334 1146 312 146
EcoICRI GAG'CTC
Cuts at: 547 3342 4715 547
Size: 2795 1373 3537
Fragments arranged by size:
3537 2795 1373
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 505 1087 1096 1252 1939 2079 2266 2381
Size: 137 582 9 156 687 140 187 115
Cuts at: 2381 2584 2812 2980 3066 3112 3160 3174 3536
Size: 203 228 168 86 46 48 14 362
Cuts at: 3536 3955 4010 4070 4190 4581 5528 5960 6709
Size: 419 55 60 120 391 947 432 749
Cuts at: 6709 6977 368
Size: 268 1096
Fragments arranged by size:
1096 947 749 687 582 432 419 391
362 268 228 203 187 168 156 140
137 120 115 86 60 55 48 46
14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2215 3565 2215
Size: 1350 6355
EcoO109I rG'GnC_Cy
Cuts at: 528 619 1449 1911 2388 2533 3034 3096 3418
Size: 91 830 462 477 145 501 62 322
Cuts at: 3418 3441 3442 3527 3558 3570 3694 3870 4228
Size: 23 1 85 31 12 124 176 358
Cuts at: 4228 4618 4619 4842 528
Size: 390 1 223 3391
Fragments arranged by size:
3391 830 501 477 462 390 358 322
223 176 145 124 91 85 62 31
23 12 1 1
EcoRI G'AATT_C
Cuts at: 4707 4707
Size: 7705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1722 3204 5722 1722
Size: 1482 2518 3705
Fragments arranged by size:
3705 2518 1482
FspI TGC'GCA
Cuts at: 2377 5484 2377
Size: 3107 4598
HaeII r_GCGC'y
Cuts at: 464 1467 1535 5137 5385 6409 7388 464
Size: 1003 68 3602 248 1024 979 781
Fragments arranged by size:
3602 1024 1003 979 781 248 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 234 266 1244 1276 1492 1524 2672
Size: 32 15 32 978 32 216 32 1148
Cuts at: 2672 2704 2732 2764 2988 3020 3743 3775 3862
Size: 32 28 32 224 32 723 32 87
Cuts at: 3862 3894 4296 4328 5545 5575 5577 5607 187
Size: 32 402 32 1217 30 2 30 2285
Fragments arranged by size:
2285 1217 1148 978 723 402 224 216
87 32 32 32 32 32 32 32
32 32 32 30 30 28 15 2
HincII GTy'rAC
Cuts at: 402 2037 4742 402
Size: 1635 2705 3365
Fragments arranged by size:
3365 2705 1635
HindIII A'AGCT_T
Cuts at: 1200 1899 2363 1200
Size: 699 464 6542
Fragments arranged by size:
6542 699 464
KasI G'GCGC_C
Cuts at: 460 5133 5381 460
Size: 4673 248 2784
Fragments arranged by size:
4673 2784 248
KpnI G_GTAC'C
Cuts at: 4723 4723
Size: 7705
MluI A'CGCG_T
Cuts at: 7138 7138
Size: 7705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1628 6376 6560 1628
Size: 4748 184 2773
Fragments arranged by size:
4748 2773 184
MscI TGG'CCA
Cuts at: 904 3235 4148 5464 904
Size: 2331 913 1316 3145
Fragments arranged by size:
3145 2331 1316 913
MslI CAynn'nnrTG
Cuts at: 412 562 3334 4513 5692 5819 412
Size: 150 2772 1179 1179 127 2298
Fragments arranged by size:
2772 2298 1179 1179 150 127
MspA1I CmG'CkG
Cuts at: 1485 1612 2235 2316 2736 2766 2784 4323 4814
Size: 127 623 81 420 30 18 1539 491
Cuts at: 4814 5128 5488 6503 6748 1485
Size: 314 360 1015 245 2442
Fragments arranged by size:
2442 1539 1015 623 491 420 360 314
245 127 81 30 18
NaeI GCC'GGC
Cuts at: 757 5884 757
Size: 5127 2578
NarI GG'CG_CC
Cuts at: 461 5134 5382 461
Size: 4673 248 2784
Fragments arranged by size:
4673 2784 248
NcoI C'CATG_G
Cuts at: 410 503 1538 3486 3788 410
Size: 93 1035 1948 302 4327
Fragments arranged by size:
4327 1948 1035 302 93
NgoMIV G'CCGG_C
Cuts at: 755 5882 755
Size: 5127 2578
NruI TCG'CGA
Cuts at: 6066 6066
Size: 7705
NspI r_CATG'y
Cuts at: 658 4221 4756 5256 6165 658
Size: 3563 535 500 909 2198
Fragments arranged by size:
3563 2198 909 535 500
PasI CC'CwG_GG
Cuts at: 865 3866 3883 3898 865
Size: 3001 17 15 4672
Fragments arranged by size:
4672 3001 17 15
PciI A'CATG_T
Cuts at: 6161 6161
Size: 7705
PflMI CCAn_nnn'nTGG
Cuts at: 724 1065 1873 2607 4144 4614 724
Size: 341 808 734 1537 470 3815
Fragments arranged by size:
3815 1537 808 734 470 341
PfoI T'CCnGG_A
Cuts at: 1058 1229 2390 2522 4597 1058
Size: 171 1161 132 2075 4166
Fragments arranged by size:
4166 2075 1161 171 132
PmeI GTTT'AAAC
Cuts at: 4702 4702
Size: 7705
PmlI CAC'GTG
Cuts at: 3081 3081
Size: 7705
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1914 1946 6844 6876 1914
Size: 32 4898 32 2743
Fragments arranged by size:
4898 2743 32 32
PpuMI rG'GwC_Cy
Cuts at: 528 619 1449 1911 2388 3034 3096 3418 3527
Size: 91 830 462 477 646 62 322 109
Cuts at: 3527 3558 3570 3870 4228 528
Size: 31 12 300 358 4005
Fragments arranged by size:
4005 830 646 477 462 358 322 300
109 91 62 31 12
PshAI GACnn'nnGTC
Cuts at: 1136 3965 1136
Size: 2829 4876
PspOMI G'GGCC_C
Cuts at: 2849 3279 3441 4618 2849
Size: 430 162 1177 5936
Fragments arranged by size:
5936 1177 430 162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4176 4208 4176
Size: 32 7673
PstI C_TGCA'G
Cuts at: 635 2735 4299 4328 4750 5435 635
Size: 2100 1564 29 422 685 2905
Fragments arranged by size:
2905 2100 1564 685 422 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7705
PvuII CAG'CTG
Cuts at: 1485 1612 2235 2736 4323 5128 5488 1485
Size: 127 623 501 1587 805 360 3702
Fragments arranged by size:
3702 1587 805 623 501 360 127
RsrII CG'GwC_CG
Cuts at: 5898 5898
Size: 7705
SacI G_AGCT'C
Cuts at: 549 3344 4717 549
Size: 2795 1373 3537
Fragments arranged by size:
3537 2795 1373
SalI G'TCGA_C
Cuts at: 4740 4740
Size: 7705
SanDI GG'GwC_CC
Cuts at: 1449 3034 3527 3870 1449
Size: 1585 493 343 5284
Fragments arranged by size:
5284 1585 493 343
SapI GCTCTTCn'nnn_
Cuts at: 495 1669 1767 2464 2695 5726 5936 495
Size: 1174 98 697 231 3031 210 2264
Fragments arranged by size:
3031 2264 1174 697 231 210 98
SbfI CC_TGCA'GG
Cuts at: 4299 4750 4299
Size: 451 7254
ScaI AGT'ACT
Cuts at: 7222 7222
Size: 7705
SexAI A'CCwGG_T
Cuts at: 2107 3554 2107
Size: 1447 6258
SfcI C'TryA_G
Cuts at: 33 631 1273 1380 2731 3112 4295 4324 4746
Size: 598 642 107 1351 381 1183 29 422
Cuts at: 4746 5431 6426 6617 33
Size: 685 995 191 1121
Fragments arranged by size:
1351 1183 1121 995 685 642 598 422
381 191 107 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 910 910
Size: 7705
SfoI GGC'GCC
Cuts at: 462 5135 5383 462
Size: 4673 248 2784
Fragments arranged by size:
4673 2784 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7705
SmaI CCC'GGG
Cuts at: 1025 2440 2854 3424 4725 1025
Size: 1415 414 570 1301 4005
Fragments arranged by size:
4005 1415 1301 570 414
SmlI C'TyrA_G
Cuts at: 1337 1969 2116 2875 3202 4239 4426 4441 4474
Size: 632 147 759 327 1037 187 15 33
Cuts at: 4474 4858 5773 6267 6529 1337
Size: 384 915 494 262 2513
Fragments arranged by size:
2513 1037 915 759 632 494 384 327
262 187 147 33 15
SnaBI TAC'GTA
Cuts at: 4695 4695
Size: 7705
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7705
SphI G_CATG'C
Cuts at: 4221 4756 5256 4221
Size: 535 500 6670
Fragments arranged by size:
6670 535 500
SspI AAT'ATT
Cuts at: 1224 1224
Size: 7705
StyI C'CwwG_G
Cuts at: 410 503 1187 1494 1538 1706 3068 3486 3543
Size: 93 684 307 44 168 1362 418 57
Cuts at: 3543 3788 3994 4482 4837 5191 410
Size: 245 206 488 355 354 2924
Fragments arranged by size:
2924 1362 684 488 418 355 354 307
245 206 168 93 57 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 3465 3598 3846 3934 4278 5286 5419 5878
Size: 2789 133 248 88 344 1008 133 459
Cuts at: 5878 6089 676
Size: 211 2292
Fragments arranged by size:
2789 2292 1008 459 344 248 211 133
133 88
TatI w'GTAC_w
Cuts at: 7220 7220
Size: 7705
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 464 496 2372 2404 7222 7254 464
Size: 32 1876 32 4818 32 915
Fragments arranged by size:
4818 1876 915 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5500 5500
Size: 7705
XbaI T'CTAG_A
Cuts at: 4734 4734
Size: 7705
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2976 4156 5198 2976
Size: 1180 1042 5483
Fragments arranged by size:
5483 1180 1042
XmaI C'CCGG_G
Cuts at: 1023 2438 2852 3422 4723 1023
Size: 1415 414 570 1301 4005
Fragments arranged by size:
4005 1415 1301 570 414
XmnI GAAnn'nnTTC
Cuts at: 812 1225 812
Size: 413 7292
ZraI GAC'GTC
Cuts at: 3314 3314
Size: 7705
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BcgI BfrBI BmtI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NsiI PacI PsiI PspXI SacII SgrAI SrfI StuI
SwaI XhoI