Restriction map for
pF1KSDA0383
(Circular) (Six-base) MAPSORT of: pF1KSDA0383.seq Check: 7075 from: 1 to: 8482
pF1KSDA0383 8482 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2364 2364
Size: 8482
AccI GT'mk_AC
Cuts at: 3686 4238 5518 3686
Size: 552 1280 6650
Fragments arranged by size:
6650 1280 552
Acc65I G'GTAC_C
Cuts at: 1403 5496 1403
Size: 4093 4389
AclI AA'CG_TT
Cuts at: 1696 8322 1696
Size: 6626 1856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 523 1337 2264 3119 3758 4677 6305 6737
Size: 293 814 927 855 639 919 1628 432
Cuts at: 6737 7486 7754 230
Size: 749 268 958
Fragments arranged by size:
1628 958 927 919 855 814 749 639
432 293 268
AflII C'TTAA_G
Cuts at: 909 909
Size: 8482
AflIII A'CryG_T
Cuts at: 3235 5832 6938 7915 3235
Size: 2597 1106 977 3802
Fragments arranged by size:
3802 2597 1106 977
AgeI A'CCGG_T
Cuts at: 6870 6870
Size: 8482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2372 4468 5129 2372
Size: 2096 661 5725
Fragments arranged by size:
5725 2096 661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 4345 4377 2306
Size: 32 2007 32 6411
Fragments arranged by size:
6411 2007 32 32
AlwNI CAG_nnn'CTG
Cuts at: 268 579 947 2242 2433 4331 4545 5056 5785
Size: 311 368 1295 191 1898 214 511 729
Cuts at: 5785 7354 268
Size: 1569 1396
Fragments arranged by size:
1898 1569 1396 1295 729 511 368 311
214 191
ApaLI G'TGCA_C
Cuts at: 7252 7252
Size: 8482
ApoI r'AATT_y
Cuts at: 874 1420 1504 1589 1643 3063 5484 7835 8091
Size: 546 84 85 54 1420 2421 2351 256
Cuts at: 8091 874
Size: 1265
Fragments arranged by size:
2421 2351 1420 1265 546 256 85 84
54
AseI AT'TA_AT
Cuts at: 20 20
Size: 8482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8482
AvaI C'yCGr_G
Cuts at: 4603 5500 4603
Size: 897 7585
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 667 700 667
Size: 33 8449
BamHI G'GATC_C
Cuts at: 1207 5505 1207
Size: 4298 4184
BanI G'GyrC_C
Cuts at: 675 1403 5335 5496 5910 6158 6193 675
Size: 728 3932 161 414 248 35 2964
Fragments arranged by size:
3932 2964 728 414 248 161 35
BanII G_rGCy'C
Cuts at: 2726 3716 5494 6524 2726
Size: 990 1778 1030 4684
Fragments arranged by size:
4684 1778 1030 990
BbeI G_GCGC'C
Cuts at: 5914 6162 5914
Size: 248 8234
BbsI GAAGACnn'nnnn_
Cuts at: 265 1397 3052 3455 265
Size: 1132 1655 403 5292
Fragments arranged by size:
5292 1655 1132 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3798 3832 3798
Size: 34 8448
BciVI GTATCCnnnnn_n'
Cuts at: 695 1765 6378 7141 8482 695
Size: 1070 4613 763 1341 695
Fragments arranged by size:
4613 1341 1070 763 695
BclI T'GATC_A
Cuts at: 1826 3468 4734 6000 1826
Size: 1642 1266 1266 4308
Fragments arranged by size:
4308 1642 1266 1266
BfrBI ATG'CAT
Cuts at: 1219 1219
Size: 8482
BglII A'GATC_T
Cuts at: 5995 8034 5995
Size: 2039 6443
BlpI GC'TnA_GC
Cuts at: 2273 5592 2273
Size: 3319 5163
Bme1580I G_kGCm'C
Cuts at: 678 2053 3243 5338 6105 6198 7256 678
Size: 1375 1190 2095 767 93 1058 1904
Fragments arranged by size:
2095 1904 1375 1190 1058 767 93
BmrI ACTGGGnnnn_n'
Cuts at: 595 683 3149 3200 4039 5692 5773 6108 8254
Size: 88 2466 51 839 1653 81 335 2146
Cuts at: 8254 595
Size: 823
Fragments arranged by size:
2466 2146 1653 839 823 335 88 81
51
BmtI G_CTAG'C
Cuts at: 2297 2297
Size: 8482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6847 6847
Size: 8482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1562 2455 2785 4365 1562
Size: 893 330 1580 5679
Fragments arranged by size:
5679 1580 893 330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 3194 3929 4487 5656 6535 7029 7327 499
Size: 2695 735 558 1169 879 494 298 1654
Fragments arranged by size:
2695 1654 1169 879 735 558 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1919 2683 8186 1919
Size: 764 5503 2215
Fragments arranged by size:
5503 2215 764
BsaAI yAC'GTr
Cuts at: 5472 5833 5472
Size: 361 8121
BsaBI GATnn'nnATC
Cuts at: 6 5994 8033 6
Size: 5988 2039 455
Fragments arranged by size:
5988 2039 455
BsaHI Gr'CG_yC
Cuts at: 3683 5911 6159 6863 8359 3683
Size: 2228 248 704 1496 3806
Fragments arranged by size:
3806 2228 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3049 5669 6190 6870 7144 7291 7702 3049
Size: 2620 521 680 274 147 411 3829
Fragments arranged by size:
3829 2620 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3365 3395 4345 4363 4375 4393 4893 4923 3365
Size: 30 950 18 12 18 500 30 6924
Fragments arranged by size:
6924 950 500 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 486 496 817 1443 2482 2485 2488 2497 2500
Size: 10 321 626 1039 3 3 9 3
Cuts at: 2500 2512 2539 3187 3190 3328 3331 3334 3337
Size: 12 27 648 3 138 3 3 3
Cuts at: 3337 3386 3493 3496 3775 4490 4895 4898 486
Size: 49 107 3 279 715 405 3 4070
Fragments arranged by size:
4070 1039 715 648 626 405 321 279
138 107 49 27 12 10 9 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 561 1835 2300 3727 3843 4415 4663 5243 5339
Size: 1274 465 1427 116 572 248 580 96
Cuts at: 5339 5351 5905 6663 7242 7738 561
Size: 12 554 758 579 496 1305
Fragments arranged by size:
1427 1305 1274 758 580 579 572 554
496 465 248 116 96 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1103 1298 3440 3661 4001 4575 5099 857
Size: 246 195 2142 221 340 574 524 4240
Fragments arranged by size:
4240 2142 574 524 340 246 221 195
BsiEI CG_ry'CG
Cuts at: 104 1159 6022 6068 7278 104
Size: 1055 4863 46 1210 1308
Fragments arranged by size:
4863 1308 1210 1055 46
BsiHKAI G_wGCw'C
Cuts at: 4276 5494 6272 6462 7256 4276
Size: 1218 778 190 794 5502
Fragments arranged by size:
5502 1218 794 778 190
BsiWI C'GTAC_G
Cuts at: 4080 4080
Size: 8482
BsmI GAATG_Cn'
Cuts at: 694 934 1024 1049 1219 1221 2388 2824 2901
Size: 240 90 25 170 2 1167 436 77
Cuts at: 2901 694
Size: 6275
Fragments arranged by size:
6275 1167 436 240 170 90 77 25
2
BsmBI CGTCTCn'nnnn_
Cuts at: 1951 2695 4600 5045 1951
Size: 744 1905 445 5388
Fragments arranged by size:
5388 1905 744 445
Bsp1286I G_dGCh'C
Cuts at: 678 2053 2726 3243 3716 4276 5338 5494 6105
Size: 1375 673 517 473 560 1062 156 611
Cuts at: 6105 6198 6272 6462 6524 7256 678
Size: 93 74 190 62 732 1904
Fragments arranged by size:
1904 1375 1062 732 673 611 560 517
473 190 156 93 74 62
BspEI T'CCGG_A
Cuts at: 7702 7702
Size: 8482
BspHI T'CATG_A
Cuts at: 1107 7658 1107
Size: 6551 1931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2364 3096 4492 5530 5744 6046 6427 2364
Size: 732 1396 1038 214 302 381 4419
Fragments arranged by size:
4419 1396 1038 732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2277 6772 6826 8312 8477 50
Size: 2227 4495 54 1486 165 55
Fragments arranged by size:
4495 2227 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 441 2060 2883 4741 6392 441
Size: 1619 823 1858 1651 2531
Fragments arranged by size:
2531 1858 1651 1619 823
BsrFI r'CCGG_y
Cuts at: 1400 2402 6478 6659 6870 1400
Size: 1002 4076 181 211 3012
Fragments arranged by size:
4076 3012 1002 211 181
BsrGI T'GTAC_A
Cuts at: 3068 5390 3068
Size: 2322 6160
BssSI C'ACGA_G
Cuts at: 6751 7111 6751
Size: 360 8122
BstAPI GCAn_nnn'nTGC
Cuts at: 579 929 938 4786 5361 5857 579
Size: 350 9 3848 575 496 3204
Fragments arranged by size:
3848 3204 575 496 350 9
BstBI TT'CG_AA
Cuts at: 110 110
Size: 8482
BstEII G'GTnAC_C
Cuts at: 1235 1235
Size: 8482
BstXI CCAn_nnnn'nTGG
Cuts at: 4590 4590
Size: 8482
BstYI r'GATC_y
Cuts at: 1 1207 3390 5505 5972 5995 6330 6576 7590
Size: 1206 2183 2115 467 23 335 246 1014
Cuts at: 7590 7676 7688 8034 1
Size: 86 12 346 449
Fragments arranged by size:
2183 2115 1206 1014 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3299 3299
Size: 8482
BtgI C'CryG_G
Cuts at: 125 243 3089 3437 3851 5376 125
Size: 118 2846 348 414 1525 3231
Fragments arranged by size:
3231 2846 1525 414 348 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6430 6611 6774 6430
Size: 181 163 8138
Fragments arranged by size:
8138 181 163
BtsI GCAGTG_nn'
Cuts at: 925 948 2243 2460 2682 5723 925
Size: 23 1295 217 222 3041 3684
Fragments arranged by size:
3684 3041 1295 222 217 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1542 1577 1542
Size: 35 8447
DraI TTT'AAA
Cuts at: 3057 5479 3057
Size: 2422 6060
DraIII CAC_nnn'GTG
Cuts at: 3268 3268
Size: 8482
DrdI GACnn_nn'nnGTC
Cuts at: 3682 6186 7046 3682
Size: 2504 860 5118
Fragments arranged by size:
5118 2504 860
EaeI y'GGCC_r
Cuts at: 1398 2404 2992 4071 6065 6239 6630 6657 8004
Size: 1006 588 1079 1994 174 391 27 1347
Cuts at: 8004 1398
Size: 1876
Fragments arranged by size:
1994 1876 1347 1079 1006 588 391 174
27
EagI C'GGCC_G
Cuts at: 6065 6065
Size: 8482
EarI CTCTTCn'nnn_
Cuts at: 206 1313 1684 1890 1963 2243 2459 2489 2501
Size: 1107 371 206 73 280 216 30 12
Cuts at: 2501 2507 2516 2528 2738 2858 3299 3305 3323
Size: 6 9 12 210 120 441 6 18
Cuts at: 3323 3332 3341 3470 3482 3491 3569 4350 6503
Size: 9 9 129 12 9 78 781 2153
Cuts at: 6503 6713 206
Size: 210 1975
Fragments arranged by size:
2153 1975 1107 781 441 371 280 216
210 210 206 129 120 78 73 30
18 12 12 12 9 9 9 9
6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6688 7000 7146 8292 6688
Size: 312 146 1146 6878
Fragments arranged by size:
6878 1146 312 146
EcoICRI GAG'CTC
Cuts at: 5492 5492
Size: 8482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 523 1337 1562 2264 2455 2785 3119 3758
Size: 293 814 225 702 191 330 334 639
Cuts at: 3758 4365 4677 6305 6737 7486 7754 230
Size: 607 312 1628 432 749 268 958
Fragments arranged by size:
1628 958 814 749 702 639 607 432
334 330 312 293 268 225 191
EcoNI CCTnn'n_nnAGG
Cuts at: 1780 1780
Size: 8482
EcoO109I rG'GnC_Cy
Cuts at: 203 342 3732 4861 5110 5619 203
Size: 139 3390 1129 249 509 3066
Fragments arranged by size:
3390 3066 1129 509 249 139
EcoRI G'AATT_C
Cuts at: 1504 5484 1504
Size: 3980 4502
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6499 6499
Size: 8482
FspI TGC'GCA
Cuts at: 6261 6261
Size: 8482
HaeII r_GCGC'y
Cuts at: 363 5088 5173 5914 6162 7186 8165 363
Size: 4725 85 741 248 1024 979 680
Fragments arranged by size:
4725 1024 979 741 680 248 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1192 1224 1850 1882 2102 2134 4192 4224 4313
Size: 32 626 32 220 32 2058 32 89
Cuts at: 4313 4345 4893 4925 6322 6352 6354 6384 1192
Size: 32 548 32 1397 30 2 30 3290
Fragments arranged by size:
3290 2058 1397 626 548 220 89 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2330 3687 4092 4718 5187 5519 2330
Size: 1357 405 626 469 332 5293
Fragments arranged by size:
5293 1357 626 469 405 332
HindIII A'AGCT_T
Cuts at: 1489 1789 3591 1489
Size: 300 1802 6380
Fragments arranged by size:
6380 1802 300
HpaI GTT'AAC
Cuts at: 4718 4718
Size: 8482
KasI G'GCGC_C
Cuts at: 5910 6158 5910
Size: 248 8234
KpnI G_GTAC'C
Cuts at: 1407 5500 1407
Size: 4093 4389
MfeI C'AATT_G
Cuts at: 467 467
Size: 8482
MluI A'CGCG_T
Cuts at: 7915 7915
Size: 8482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 1749 4010 4451 4504 7153 7337 230
Size: 1519 2261 441 53 2649 184 1375
Fragments arranged by size:
2649 2261 1519 1375 441 184 53
MscI TGG'CCA
Cuts at: 2994 4073 6241 2994
Size: 1079 2168 5235
Fragments arranged by size:
5235 2168 1079
MslI CAynn'nnrTG
Cuts at: 2000 4159 5020 5146 5152 5203 5428 6469 6596
Size: 2159 861 126 6 51 225 1041 127
Cuts at: 6596 2000
Size: 3886
Fragments arranged by size:
3886 2159 1041 861 225 127 126 51
6
MspA1I CmG'CkG
Cuts at: 276 576 2430 2763 4358 4403 4436 4545 4590
Size: 300 1854 333 1595 45 33 109 45
Cuts at: 4590 4762 5056 5591 5905 6265 7280 7525 276
Size: 172 294 535 314 360 1015 245 1233
Fragments arranged by size:
1854 1595 1233 1015 535 360 333 314
300 294 245 172 109 45 45 33
NaeI GCC'GGC
Cuts at: 2404 6661 2404
Size: 4257 4225
NarI GG'CG_CC
Cuts at: 5911 6159 5911
Size: 248 8234
NcoI C'CATG_G
Cuts at: 125 243 3089 3851 125
Size: 118 2846 762 4756
Fragments arranged by size:
4756 2846 762 118
NdeI CA'TA_TG
Cuts at: 1013 5346 1013
Size: 4333 4149
NgoMIV G'CCGG_C
Cuts at: 2402 6659 2402
Size: 4257 4225
NheI G'CTAG_C
Cuts at: 2293 2293
Size: 8482
NruI TCG'CGA
Cuts at: 6843 6843
Size: 8482
NsiI A_TGCA'T
Cuts at: 1221 1221
Size: 8482
NspI r_CATG'y
Cuts at: 941 2049 3239 4452 5241 5433 5533 6033 6942
Size: 1108 1190 1213 789 192 100 500 909
Cuts at: 6942 941
Size: 2481
Fragments arranged by size:
2481 1213 1190 1108 909 789 500 192
100
PasI CC'CwG_GG
Cuts at: 355 4855 355
Size: 4500 3982
PciI A'CATG_T
Cuts at: 3235 6938 3235
Size: 3703 4779
PflMI CCAn_nnn'nTGG
Cuts at: 249 4545 249
Size: 4296 4186
PfoI T'CCnGG_A
Cuts at: 1682 1682
Size: 8482
PmeI GTTT'AAAC
Cuts at: 5479 5479
Size: 8482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4345 4377 7621 7653 4345
Size: 32 3244 32 5174
Fragments arranged by size:
5174 3244 32 32
PpuMI rG'GwC_Cy
Cuts at: 342 3732 4861 5110 342
Size: 3390 1129 249 3714
Fragments arranged by size:
3714 3390 1129 249
PshAI GACnn'nnGTC
Cuts at: 3989 3989
Size: 8482
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5248 5280 5248
Size: 32 8450
PstI C_TGCA'G
Cuts at: 1078 2078 2238 4175 4447 4457 4489 4712 5527
Size: 1000 160 1937 272 10 32 223 815
Cuts at: 5527 6212 1078
Size: 685 3348
Fragments arranged by size:
3348 1937 1000 815 685 272 223 160
32 10
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8482
PvuII CAG'CTG
Cuts at: 276 2430 2763 4358 4403 4545 4590 5905 6265
Size: 2154 333 1595 45 142 45 1315 360
Cuts at: 6265 276
Size: 2493
Fragments arranged by size:
2493 2154 1595 1315 360 333 142 45
45
RsrII CG'GwC_CG
Cuts at: 631 6675 631
Size: 6044 2438
SacI G_AGCT'C
Cuts at: 5494 5494
Size: 8482
SalI G'TCGA_C
Cuts at: 3685 5517 3685
Size: 1832 6650
SanDI GG'GwC_CC
Cuts at: 3732 4861 3732
Size: 1129 7353
SapI GCTCTTCn'nnn_
Cuts at: 1684 1963 2243 3491 3569 4350 6503 6713 1684
Size: 279 280 1248 78 781 2153 210 3453
Fragments arranged by size:
3453 2153 1248 781 280 279 210 78
SbfI CC_TGCA'GG
Cuts at: 5527 5527
Size: 8482
ScaI AGT'ACT
Cuts at: 237 2714 3605 7999 237
Size: 2477 891 4394 720
Fragments arranged by size:
4394 2477 891 720
SexAI A'CCwGG_T
Cuts at: 1444 3264 1444
Size: 1820 6662
SfcI C'TryA_G
Cuts at: 33 948 1074 2074 2234 3954 3964 4171 4365
Size: 915 126 1000 160 1720 10 207 194
Cuts at: 4365 4443 4453 4485 4708 5096 5523 6208 7203
Size: 78 10 32 223 388 427 685 995
Cuts at: 7203 7394 33
Size: 191 1121
Fragments arranged by size:
1720 1121 1000 995 915 685 427 388
223 207 194 191 160 126 78 32
10 10
SfoI GGC'GCC
Cuts at: 5912 6160 5912
Size: 248 8234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8482
SmaI CCC'GGG
Cuts at: 5502 5502
Size: 8482
SmlI C'TyrA_G
Cuts at: 478 909 3209 3944 4502 5635 6550 7044 7306
Size: 431 2300 735 558 1133 915 494 262
Cuts at: 7306 478
Size: 1654
Fragments arranged by size:
2300 1654 1133 915 735 558 494 431
262
SnaBI TAC'GTA
Cuts at: 5472 5472
Size: 8482
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8482
SphI G_CATG'C
Cuts at: 4452 5533 6033 4452
Size: 1081 500 6901
Fragments arranged by size:
6901 1081 500
SspI AAT'ATT
Cuts at: 1536 2383 2565 4962 1536
Size: 847 182 2397 5056
Fragments arranged by size:
5056 2397 847 182
StuI AGG'CCT
Cuts at: 326 907 2625 3952 4524 326
Size: 581 1718 1327 572 4284
Fragments arranged by size:
4284 1718 1327 581 572
StyI C'CwwG_G
Cuts at: 125 243 401 957 3089 3716 3851 5614 5968
Size: 118 158 556 2132 627 135 1763 354
Cuts at: 5968 125
Size: 2639
Fragments arranged by size:
2639 2132 1763 627 556 354 158 135
118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 875 1173 3102 4429 4596 5077 5353 6063 6196
Size: 298 1929 1327 167 481 276 710 133
Cuts at: 6196 6655 6866 875
Size: 459 211 2491
Fragments arranged by size:
2491 1929 1327 710 481 459 298 276
211 167 133
TatI w'GTAC_w
Cuts at: 235 638 2364 2631 2712 3068 3603 5390 7997
Size: 403 1726 267 81 356 535 1787 2607
Cuts at: 7997 235
Size: 720
Fragments arranged by size:
2607 1787 1726 720 535 403 356 267
81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4835 4867 7999 8031 4835
Size: 32 3132 32 5286
Fragments arranged by size:
5286 3132 32 32
Tth111I GACn'n_nGTC
Cuts at: 6277 6277
Size: 8482
XbaI T'CTAG_A
Cuts at: 409 1891 5511 409
Size: 1482 3620 3380
Fragments arranged by size:
3620 3380 1482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1055 1680 2836 4066 4194 5275 5975 1055
Size: 625 1156 1230 128 1081 700 3562
Fragments arranged by size:
3562 1230 1156 1081 700 625 128
XmaI C'CCGG_G
Cuts at: 5500 5500
Size: 8482
XmnI GAAnn'nnTTC
Cuts at: 1219 2564 1219
Size: 1345 7137
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI ApaI AscI AvrII BbvCI
BglI BmgBI Bpu10I BssHII BstZ17I ClaI EcoRV FseI
FspAI NotI PacI PmlI PsiI PspOMI PspXI SacII
SfiI SgrAI SrfI SwaI XhoI ZraI