Restriction map for
pF1KSDA0385
(Circular) (Six-base) MAPSORT of: pF1KSDA0385.seq Check: 1202 from: 1 to: 7249
pF1KSDA0385 7249 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 203 1823 3158 3202 3731 4285 203
Size: 1620 1335 44 529 554 3167
Fragments arranged by size:
3167 1620 1335 554 529 44
Acc65I G'GTAC_C
Cuts at: 495 4263 495
Size: 3768 3481
AclI AA'CG_TT
Cuts at: 7089 7089
Size: 7249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1902 2902 5072 5504 6253 6521 689
Size: 1213 1000 2170 432 749 268 1417
Fragments arranged by size:
2170 1417 1213 1000 749 432 268
AflIII A'CryG_T
Cuts at: 1603 1605 1662 4599 5705 6682 1603
Size: 2 57 2937 1106 977 2170
Fragments arranged by size:
2937 2170 1106 977 57 2
AgeI A'CCGG_T
Cuts at: 5637 5637
Size: 7249
AhdI GACnn_n'nnGTC
Cuts at: 1514 1514
Size: 7249
AleI CACnn'nnGTG
Cuts at: 1606 2330 3829 3877 1606
Size: 724 1499 48 4978
Fragments arranged by size:
4978 1499 724 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1129 2272 1129
Size: 1143 6106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1942 1974 1942
Size: 32 7217
AlwNI CAG_nnn'CTG
Cuts at: 514 1769 1847 2219 2950 3451 4552 6121 514
Size: 1255 78 372 731 501 1101 1569 1642
Fragments arranged by size:
1642 1569 1255 1101 731 501 372 78
ApaI G_GGCC'C
Cuts at: 264 2423 264
Size: 2159 5090
ApaLI G'TGCA_C
Cuts at: 1058 6019 1058
Size: 4961 2288
ApoI r'AATT_y
Cuts at: 211 522 1441 2526 4027 4251 6602 6858 211
Size: 311 919 1085 1501 224 2351 256 602
Fragments arranged by size:
2351 1501 1085 919 602 311 256 224
AseI AT'TA_AT
Cuts at: 20 3146 20
Size: 3126 4123
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7249
AvaI C'yCGr_G
Cuts at: 251 285 1167 2422 3047 3272 3686 3863 4267
Size: 34 882 1255 625 225 414 177 404
Cuts at: 4267 251
Size: 3233
Fragments arranged by size:
3233 1255 882 625 414 404 225 177
34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1594 1627 1594
Size: 33 7216
BamHI G'GATC_C
Cuts at: 1329 4272 1329
Size: 2943 4306
BanI G'GyrC_C
Cuts at: 495 1042 2282 2787 2811 3868 4263 4677 4925
Size: 547 1240 505 24 1057 395 414 248
Cuts at: 4925 4960 495
Size: 35 2784
Fragments arranged by size:
2784 1240 1057 547 505 414 395 248
35 24
BanII G_rGCy'C
Cuts at: 264 294 640 685 763 1532 1742 2423 3311
Size: 30 346 45 78 769 210 681 888
Cuts at: 3311 3458 4261 5291 264
Size: 147 803 1030 2222
Fragments arranged by size:
2222 1030 888 803 769 681 346 210
147 78 45 30
BbeI G_GCGC'C
Cuts at: 4681 4929 4681
Size: 248 7001
BbsI GAAGACnn'nnnn_
Cuts at: 1372 2359 2554 3029 4232 1372
Size: 987 195 475 1203 4389
Fragments arranged by size:
4389 1203 987 475 195
BbvCI CC'TCA_GC
Cuts at: 1118 2951 3800 1118
Size: 1833 849 4567
Fragments arranged by size:
4567 1833 849
BciVI GTATCCnnnnn_n'
Cuts at: 2400 5145 5908 7249 2400
Size: 2745 763 1341 2400
Fragments arranged by size:
2745 2400 1341 763
BclI T'GATC_A
Cuts at: 4767 4767
Size: 7249
BfrBI ATG'CAT
Cuts at: 2763 2763
Size: 7249
BglI GCCn_nnn'nGGC
Cuts at: 972 972
Size: 7249
BglII A'GATC_T
Cuts at: 1202 4762 6801 1202
Size: 3560 2039 1650
Fragments arranged by size:
3560 2039 1650
BlpI GC'TnA_GC
Cuts at: 3444 3452 4359 3444
Size: 8 907 6334
Fragments arranged by size:
6334 907 8
Bme1580I G_kGCm'C
Cuts at: 264 1045 1062 2423 2741 2822 3871 4872 4965
Size: 781 17 1361 318 81 1049 1001 93
Cuts at: 4965 6023 264
Size: 1058 1490
Fragments arranged by size:
1490 1361 1058 1049 1001 781 318 93
81 17
BmrI ACTGGGnnnn_n'
Cuts at: 128 140 1314 2013 2728 3146 3468 3733 3898
Size: 12 1174 699 715 418 322 265 165
Cuts at: 3898 4216 4459 4540 4875 7021 128
Size: 318 243 81 335 2146 356
Fragments arranged by size:
2146 1174 715 699 418 356 335 322
318 265 243 165 81 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 630 5614 630
Size: 4984 2265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 356 545 1265 1391 2335 209
Size: 147 189 720 126 944 5123
Fragments arranged by size:
5123 944 720 189 147 126
Bpu10I CC'TnA_GC
Cuts at: 1118 1526 1891 2951 3800 1118
Size: 408 365 1060 849 4567
Fragments arranged by size:
4567 1060 849 408 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 990 4423 5302 5796 6094 990
Size: 3433 879 494 298 2145
Fragments arranged by size:
3433 2145 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 185 601 2311 2860 6953 185
Size: 416 1710 549 4093 481
Fragments arranged by size:
4093 1710 549 481 416
BsaAI yAC'GTr
Cuts at: 1606 4239 4600 1606
Size: 2633 361 4255
Fragments arranged by size:
4255 2633 361
BsaBI GATnn'nnATC
Cuts at: 6 2803 4761 6800 6
Size: 2797 1958 2039 455
Fragments arranged by size:
2797 2039 1958 455
BsaHI Gr'CG_yC
Cuts at: 2654 4678 4926 5630 7126 2654
Size: 2024 248 704 1496 2777
Fragments arranged by size:
2777 2024 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3575 4058 4436 4957 5637 5911 6058 6469 3575
Size: 483 378 521 680 274 147 411 4355
Fragments arranged by size:
4355 680 521 483 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1734 1764 2662 2692 1734
Size: 30 898 30 6291
Fragments arranged by size:
6291 898 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 619 667 670 977 1522 2890 2968 3652
Size: 3 48 3 307 545 1368 78 684
Cuts at: 3652 3709 3712 3739 616
Size: 57 3 27 4126
Fragments arranged by size:
4126 1368 684 545 307 78 57 48
27 3 3 3
BseYI C'CCAG_C
Cuts at: 363 369 514 1298 1840 2139 2595 3344 4672
Size: 6 145 784 542 299 456 749 1328
Cuts at: 4672 5430 6009 6505 363
Size: 758 579 496 1107
Fragments arranged by size:
1328 1107 784 758 749 579 542 496
456 299 145 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1251 1366 3026 3370 758
Size: 493 115 1660 344 4637
Fragments arranged by size:
4637 1660 493 344 115
BsiEI CG_ry'CG
Cuts at: 104 4789 4835 6045 104
Size: 4685 46 1210 1308
Fragments arranged by size:
4685 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1062 1532 2030 2851 3458 3704 4261 5039 5229
Size: 470 498 821 607 246 557 778 190
Cuts at: 5229 6023 1062
Size: 794 2288
Fragments arranged by size:
2288 821 794 778 607 557 498 470
246 190
BsmI GAATG_Cn'
Cuts at: 902 1883 902
Size: 981 6268
BsmBI CGTCTCn'nnnn_
Cuts at: 2661 2661
Size: 7249
Bsp1286I G_dGCh'C
Cuts at: 264 294 640 685 763 1045 1062 1532 1742
Size: 30 346 45 78 282 17 470 210
Cuts at: 1742 2030 2423 2741 2822 2851 3311 3458 3704
Size: 288 393 318 81 29 460 147 246
Cuts at: 3704 3871 4261 4872 4965 5039 5229 5291 6023
Size: 167 390 611 93 74 190 62 732
Cuts at: 6023 264
Size: 1490
Fragments arranged by size:
1490 732 611 470 460 393 390 346
318 288 282 246 210 190 167 147
93 81 78 74 62 45 30 29
17
BspEI T'CCGG_A
Cuts at: 4058 6469 4058
Size: 2411 4838
BspHI T'CATG_A
Cuts at: 6425 6425
Size: 7249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 1582 2188 2264 4091 4297 4511 4813 5194
Size: 1274 606 76 1827 206 214 302 381
Cuts at: 5194 308
Size: 2363
Fragments arranged by size:
2363 1827 1274 606 381 302 214 206
76
BsrBI CCG'CTC
Cuts at: 50 5539 5593 7079 7244 50
Size: 5489 54 1486 165 55
Fragments arranged by size:
5489 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1062 1401 5159 1062
Size: 339 3758 3152
Fragments arranged by size:
3758 3152 339
BsrFI r'CCGG_y
Cuts at: 1412 5245 5426 5637 1412
Size: 3833 181 211 3024
Fragments arranged by size:
3833 3024 211 181
BsrGI T'GTAC_A
Cuts at: 2114 2114
Size: 7249
BssSI C'ACGA_G
Cuts at: 1384 1540 5518 5878 1384
Size: 156 3978 360 2755
Fragments arranged by size:
3978 2755 360 156
BstAPI GCAn_nnn'nTGC
Cuts at: 442 1358 1769 3561 4624 442
Size: 916 411 1792 1063 3067
Fragments arranged by size:
3067 1792 1063 916 411
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7249
BstXI CCAn_nnnn'nTGG
Cuts at: 1369 1622 2332 2841 2907 3090 3879 1369
Size: 253 710 509 66 183 789 4739
Fragments arranged by size:
4739 789 710 509 253 183 66
BstYI r'GATC_y
Cuts at: 1 222 1202 1329 2339 2537 2891 4272 4739
Size: 221 980 127 1010 198 354 1381 467
Cuts at: 4739 4762 5097 5343 6357 6443 6455 6801 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1381 1014 1010 980 467 449 354 346
335 246 221 198 127 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 422 488 536 878 1874 1931 3191 422
Size: 66 48 342 996 57 1260 4480
Fragments arranged by size:
4480 1260 996 342 66 57 48
BtgI C'CryG_G
Cuts at: 125 414 932 3065 4139 125
Size: 289 518 2133 1074 3235
Fragments arranged by size:
3235 2133 1074 518 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4089 5197 5378 5541 4089
Size: 1108 181 163 5797
Fragments arranged by size:
5797 1108 181 163
BtsI GCAGTG_nn'
Cuts at: 577 1043 1075 2238 2368 2564 4490 577
Size: 466 32 1163 130 196 1926 3336
Fragments arranged by size:
3336 1926 1163 466 196 130 32
DraI TTT'AAA
Cuts at: 4246 4246
Size: 7249
DrdI GACnn_nn'nnGTC
Cuts at: 337 1819 4953 5813 337
Size: 1482 3134 860 1773
Fragments arranged by size:
3134 1773 1482 860
EaeI y'GGCC_r
Cuts at: 3062 3080 4832 5006 5397 5424 6771 3062
Size: 18 1752 174 391 27 1347 3540
Fragments arranged by size:
3540 1752 1347 391 174 27 18
EagI C'GGCC_G
Cuts at: 4832 4832
Size: 7249
EarI CTCTTCn'nnn_
Cuts at: 593 832 1129 1540 1921 2693 3943 5270 5480
Size: 239 297 411 381 772 1250 1327 210
Cuts at: 5480 593
Size: 2362
Fragments arranged by size:
2362 1327 1250 772 411 381 297 239
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3898 5455 5767 5913 7059 3898
Size: 1557 312 146 1146 4088
Fragments arranged by size:
4088 1557 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1530 3456 4259 1530
Size: 1926 803 4520
Fragments arranged by size:
4520 1926 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 356 545 689 1265 1391 1902 2335 2902
Size: 147 189 144 576 126 511 433 567
Cuts at: 2902 5072 5504 6253 6521 209
Size: 2170 432 749 268 937
Fragments arranged by size:
2170 937 749 576 567 511 433 432
268 189 147 144 126
EcoNI CCTnn'n_nnAGG
Cuts at: 470 506 1291 1384 470
Size: 36 785 93 6335
Fragments arranged by size:
6335 785 93 36
EcoO109I rG'GnC_Cy
Cuts at: 261 1377 2507 3187 3934 4386 261
Size: 1116 1130 680 747 452 3124
Fragments arranged by size:
3124 1130 1116 747 680 452
EcoRI G'AATT_C
Cuts at: 4027 4251 4027
Size: 224 7025
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1704 2160 5266 1704
Size: 456 3106 3687
Fragments arranged by size:
3687 3106 456
FspI TGC'GCA
Cuts at: 5028 5028
Size: 7249
HaeII r_GCGC'y
Cuts at: 984 1032 1311 2301 4681 4929 5953 6932 984
Size: 48 279 990 2380 248 1024 979 1301
Fragments arranged by size:
2380 1301 1024 990 979 279 248 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1083 1115 1500 1532 1642 1674 2457 2489 2631
Size: 32 385 32 110 32 783 32 142
Cuts at: 2631 2662 2663 2694 2953 2985 3041 3073 3184
Size: 31 1 31 259 32 56 32 111
Cuts at: 3184 3216 3222 3254 5089 5119 5121 5151 1083
Size: 32 6 32 1835 30 2 30 3181
Fragments arranged by size:
3181 1835 783 385 259 142 111 110
56 32 32 32 32 32 32 32
32 31 31 30 30 6 2 1
HincII GTy'rAC
Cuts at: 1812 4286 1812
Size: 2474 4775
HindIII A'AGCT_T
Cuts at: 2085 2085
Size: 7249
KasI G'GCGC_C
Cuts at: 4677 4925 4677
Size: 248 7001
KpnI G_GTAC'C
Cuts at: 499 4267 499
Size: 3768 3481
MluI A'CGCG_T
Cuts at: 6682 6682
Size: 7249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 4136 5920 6104 2924
Size: 1212 1784 184 4069
Fragments arranged by size:
4069 1784 1212 184
MscI TGG'CCA
Cuts at: 3064 3082 5008 3064
Size: 18 1926 5305
Fragments arranged by size:
5305 1926 18
MslI CAynn'nnrTG
Cuts at: 907 1048 1606 2330 2734 2746 3760 3829 3877
Size: 141 558 724 404 12 1014 69 48
Cuts at: 3877 5236 5363 907
Size: 1359 127 2793
Fragments arranged by size:
2793 1359 1014 724 558 404 141 127
69 48 12
MspA1I CmG'CkG
Cuts at: 1844 2072 3448 3799 4007 4358 4672 5032 6047
Size: 228 1376 351 208 351 314 360 1015
Cuts at: 6047 6292 1844
Size: 245 2801
Fragments arranged by size:
2801 1376 1015 360 351 351 314 245
228 208
NaeI GCC'GGC
Cuts at: 5428 5428
Size: 7249
NarI GG'CG_CC
Cuts at: 4678 4926 4678
Size: 248 7001
NcoI C'CATG_G
Cuts at: 125 932 3065 4139 125
Size: 807 2133 1074 3235
Fragments arranged by size:
3235 2133 1074 807
NdeI CA'TA_TG
Cuts at: 1357 3321 1357
Size: 1964 5285
NgoMIV G'CCGG_C
Cuts at: 5426 5426
Size: 7249
NruI TCG'CGA
Cuts at: 5610 5610
Size: 7249
NsiI A_TGCA'T
Cuts at: 2765 2765
Size: 7249
NspI r_CATG'y
Cuts at: 996 1666 3813 4155 4167 4300 4800 5709 996
Size: 670 2147 342 12 133 500 909 2536
Fragments arranged by size:
2536 2147 909 670 500 342 133 12
PasI CC'CwG_GG
Cuts at: 643 643
Size: 7249
PciI A'CATG_T
Cuts at: 1662 5705 1662
Size: 4043 3206
PflMI CCAn_nnn'nTGG
Cuts at: 514 796 1847 2327 514
Size: 282 1051 480 5436
Fragments arranged by size:
5436 1051 480 282
PfoI T'CCnGG_A
Cuts at: 341 3017 3936 341
Size: 2676 919 3654
Fragments arranged by size:
3654 2676 919
PmeI GTTT'AAAC
Cuts at: 4246 4246
Size: 7249
PmlI CAC'GTG
Cuts at: 1606 1606
Size: 7249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6388 6420 6388
Size: 32 7217
PpuMI rG'GwC_Cy
Cuts at: 1377 2507 3934 1377
Size: 1130 1427 4692
Fragments arranged by size:
4692 1427 1130
PshAI GACnn'nnGTC
Cuts at: 416 3932 416
Size: 3516 3733
PspOMI G'GGCC_C
Cuts at: 260 2419 260
Size: 2159 5090
PspXI vC'TCGA_Gb
Cuts at: 251 251
Size: 7249
PstI C_TGCA'G
Cuts at: 584 1038 1082 1354 1423 1765 1837 2095 2233
Size: 454 44 272 69 342 72 258 138
Cuts at: 2233 3794 4294 4979 584
Size: 1561 500 685 2854
Fragments arranged by size:
2854 1561 685 500 454 342 272 258
138 72 69 44
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7249
PvuII CAG'CTG
Cuts at: 1844 3448 3799 4672 5032 1844
Size: 1604 351 873 360 4061
Fragments arranged by size:
4061 1604 873 360 351
RsrII CG'GwC_CG
Cuts at: 5442 5442
Size: 7249
SacI G_AGCT'C
Cuts at: 1532 3458 4261 1532
Size: 1926 803 4520
Fragments arranged by size:
4520 1926 803
SalI G'TCGA_C
Cuts at: 4284 4284
Size: 7249
SapI GCTCTTCn'nnn_
Cuts at: 1129 5270 5480 1129
Size: 4141 210 2898
Fragments arranged by size:
4141 2898 210
SbfI CC_TGCA'GG
Cuts at: 4294 4294
Size: 7249
ScaI AGT'ACT
Cuts at: 3559 6766 3559
Size: 3207 4042
SfcI C'TryA_G
Cuts at: 33 580 1034 1078 1350 1419 1761 1833 2091
Size: 547 454 44 272 69 342 72 258
Cuts at: 2091 2229 2379 3790 4290 4975 5970 6161 33
Size: 138 150 1411 500 685 995 191 1121
Fragments arranged by size:
1411 1121 995 685 547 500 454 342
272 258 191 150 138 72 69 44
SfoI GGC'GCC
Cuts at: 4679 4927 4679
Size: 248 7001
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7249
SmaI CCC'GGG
Cuts at: 3274 4269 3274
Size: 995 6254
SmlI C'TyrA_G
Cuts at: 251 1005 3047 3686 4402 5317 5811 6073 251
Size: 754 2042 639 716 915 494 262 1427
Fragments arranged by size:
2042 1427 915 754 716 639 494 262
SnaBI TAC'GTA
Cuts at: 4239 4239
Size: 7249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7249
SphI G_CATG'C
Cuts at: 4167 4300 4800 4167
Size: 133 500 6616
Fragments arranged by size:
6616 500 133
StuI AGG'CCT
Cuts at: 2968 2968
Size: 7249
StyI C'CwwG_G
Cuts at: 125 666 932 1214 2840 2906 3065 3875 4139
Size: 541 266 282 1626 66 159 810 264
Cuts at: 4139 4381 4735 125
Size: 242 354 2639
Fragments arranged by size:
2639 1626 810 541 354 282 266 264
242 159 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3336 4830 4963 5422 5633 3336
Size: 1494 133 459 211 4952
Fragments arranged by size:
4952 1494 459 211 133
TatI w'GTAC_w
Cuts at: 2114 2161 2768 3557 6764 2114
Size: 47 607 789 3207 2599
Fragments arranged by size:
3207 2599 789 607 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1734 1766 6766 6798 1734
Size: 32 5000 32 2185
Fragments arranged by size:
5000 2185 32 32
Tth111I GACn'n_nGTC
Cuts at: 5044 5044
Size: 7249
XbaI T'CTAG_A
Cuts at: 4278 4278
Size: 7249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 254 360 4742 254
Size: 106 4382 2761
Fragments arranged by size:
4382 2761 106
XhoI C'TCGA_G
Cuts at: 251 3047 3686 251
Size: 2796 639 3814
Fragments arranged by size:
3814 2796 639
XmaI C'CCGG_G
Cuts at: 3272 4267 3272
Size: 995 6254
XmnI GAAnn'nnTTC
Cuts at: 2086 3589 2086
Size: 1503 5746
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AscI AvrII BcgI BmgBI
BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NheI NotI PacI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI ZraI