Restriction map for
pF1KSDA0387
(Circular) (Six-base) MAPSORT of: pF1KSDA0387.seq Check: 9106 from: 1 to: 6253
pF1KSDA0387 6253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 665 1797 665
Size: 1132 5121
AatII G_ACGT'C
Cuts at: 1793 1793
Size: 6253
AccI GT'mk_AC
Cuts at: 3289 3289
Size: 6253
Acc65I G'GTAC_C
Cuts at: 3267 3267
Size: 6253
AclI AA'CG_TT
Cuts at: 6093 6093
Size: 6253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 1147 1487 2155 2431 2581 2890 4076 4508
Size: 572 340 668 276 150 309 1186 432
Cuts at: 4508 5257 5525 575
Size: 749 268 1303
Fragments arranged by size:
1303 1186 749 668 572 432 340 309
276 268 150
AfeI AGC'GCT
Cuts at: 2447 2447
Size: 6253
AflIII A'CryG_T
Cuts at: 788 2252 3603 4709 5686 788
Size: 1464 1351 1106 977 1355
Fragments arranged by size:
1464 1355 1351 1106 977
AgeI A'CCGG_T
Cuts at: 4641 4641
Size: 6253
AhdI GACnn_n'nnGTC
Cuts at: 1413 1413
Size: 6253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2806 2838 2806
Size: 32 6221
AlwNI CAG_nnn'CTG
Cuts at: 288 438 2199 3556 5125 288
Size: 150 1761 1357 1569 1416
Fragments arranged by size:
1761 1569 1416 1357 150
ApaI G_GGCC'C
Cuts at: 818 1634 2248 818
Size: 816 614 4823
Fragments arranged by size:
4823 816 614
ApaLI G'TGCA_C
Cuts at: 1004 5023 1004
Size: 4019 2234
ApoI r'AATT_y
Cuts at: 1958 3255 5606 5862 1958
Size: 1297 2351 256 2349
Fragments arranged by size:
2351 2349 1297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6253
AvaI C'yCGr_G
Cuts at: 316 811 967 1238 1390 1588 1762 2148 2549
Size: 495 156 271 152 198 174 386 401
Cuts at: 2549 2625 3271 316
Size: 76 646 3298
Fragments arranged by size:
3298 646 495 401 386 271 198 174
156 152 76
BamHI G'GATC_C
Cuts at: 3276 3276
Size: 6253
BanI G'GyrC_C
Cuts at: 374 1035 1809 3052 3267 3681 3929 3964 374
Size: 661 774 1243 215 414 248 35 2663
Fragments arranged by size:
2663 1243 774 661 414 248 215 35
BanII G_rGCy'C
Cuts at: 818 881 1437 1589 1613 1634 2248 2471 3265
Size: 63 556 152 24 21 614 223 794
Cuts at: 3265 4295 818
Size: 1030 2776
Fragments arranged by size:
2776 1030 794 614 556 223 152 63
24 21
BbeI G_GCGC'C
Cuts at: 3685 3933 3685
Size: 248 6005
BbsI GAAGACnn'nnnn_
Cuts at: 1088 1484 1493 2030 1088
Size: 396 9 537 5311
Fragments arranged by size:
5311 537 396 9
BbvCI CC'TCA_GC
Cuts at: 740 1151 1678 3203 740
Size: 411 527 1525 3790
Fragments arranged by size:
3790 1525 527 411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1806 1840 2152 2186 1806
Size: 34 312 34 5873
Fragments arranged by size:
5873 312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4149 4912 6253 4149
Size: 763 1341 4149
Fragments arranged by size:
4149 1341 763
BclI T'GATC_A
Cuts at: 3771 3771
Size: 6253
BglI GCCn_nnn'nGGC
Cuts at: 1169 2461 2674 3050 1169
Size: 1292 213 376 4372
Fragments arranged by size:
4372 1292 376 213
BglII A'GATC_T
Cuts at: 1557 3766 5805 1557
Size: 2209 2039 2005
Fragments arranged by size:
2209 2039 2005
BlpI GC'TnA_GC
Cuts at: 880 1193 3363 880
Size: 313 2170 3770
Fragments arranged by size:
3770 2170 313
Bme1580I G_kGCm'C
Cuts at: 818 1008 1634 1814 2248 2510 3876 3969 5027
Size: 190 626 180 434 262 1366 93 1058
Cuts at: 5027 818
Size: 2044
Fragments arranged by size:
2044 1366 1058 626 434 262 190 180
93
BmgBI CAC'GTC
Cuts at: 699 791 1833 2255 699
Size: 92 1042 422 4697
Fragments arranged by size:
4697 1042 422 92
BmrI ACTGGGnnnn_n'
Cuts at: 2274 3231 3463 3544 3879 6025 2274
Size: 957 232 81 335 2146 2502
Fragments arranged by size:
2502 2146 957 335 232 81
BmtI G_CTAG'C
Cuts at: 2606 2606
Size: 6253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1962 2163 4618 1962
Size: 201 2455 3597
Fragments arranged by size:
3597 2455 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 850 1003 1195 1295 1451 1957 2449 3148
Size: 162 153 192 100 156 506 492 699
Cuts at: 3148 688
Size: 3793
Fragments arranged by size:
3793 699 506 492 192 162 156 153
100
Bpu10I CC'TnA_GC
Cuts at: 740 1151 1667 1678 3203 740
Size: 411 516 11 1525 3790
Fragments arranged by size:
3790 1525 516 411 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 1978 2937 3156 3208 3427 4306 4800 5098
Size: 982 959 219 52 219 879 494 298
Cuts at: 5098 996
Size: 2151
Fragments arranged by size:
2151 982 959 879 494 298 219 219
52
BsaI GGTCTCn'nnnn_
Cuts at: 1421 1742 2061 2826 3141 5957 1421
Size: 321 319 765 315 2816 1717
Fragments arranged by size:
2816 1717 765 321 319 315
BsaAI yAC'GTr
Cuts at: 479 3243 3604 479
Size: 2764 361 3128
Fragments arranged by size:
3128 2764 361
BsaBI GATnn'nnATC
Cuts at: 6 3112 3765 5804 6
Size: 3106 653 2039 455
Fragments arranged by size:
3106 2039 653 455
BsaHI Gr'CG_yC
Cuts at: 549 651 1790 2078 2750 3682 3930 4634 6130
Size: 102 1139 288 672 932 248 704 1496
Cuts at: 6130 549
Size: 672
Fragments arranged by size:
1496 1139 932 704 672 672 288 248
102
BsaWI w'CCGG_w
Cuts at: 1969 3440 3961 4641 4915 5062 5473 1969
Size: 1471 521 680 274 147 411 2749
Fragments arranged by size:
2749 1471 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 597 627 661 691 742 772 792 822 976
Size: 30 34 30 51 30 20 30 154
Cuts at: 976 1006 2422 2452 2806 2836 597
Size: 30 1416 30 354 30 4014
Fragments arranged by size:
4014 1416 354 154 51 34 30 30
30 30 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 618 1525 1657 1997 2356 2512 2871 2940
Size: 284 907 132 340 359 156 359 69
Cuts at: 2940 2951 3220 334
Size: 11 269 3367
Fragments arranged by size:
3367 907 359 359 340 284 269 156
132 69 11
BseYI C'CCAG_C
Cuts at: 567 687 824 957 1563 1813 2302 3676 4434
Size: 120 137 133 606 250 489 1374 758
Cuts at: 4434 5013 5509 567
Size: 579 496 1311
Fragments arranged by size:
1374 1311 758 606 579 496 489 250
137 133 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1357 2192 2371 3059 313
Size: 1044 835 179 688 3507
Fragments arranged by size:
3507 1044 835 688 179
BsiEI CG_ry'CG
Cuts at: 104 3793 3839 5049 104
Size: 3689 46 1210 1308
Fragments arranged by size:
3689 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 704 881 1008 1110 1484 1613 1668 2831 3134
Size: 177 127 102 374 129 55 1163 303
Cuts at: 3134 3265 4043 4233 5027 704
Size: 131 778 190 794 1930
Fragments arranged by size:
1930 1163 794 778 374 303 190 177
131 129 127 102 55
BsmI GAATG_Cn'
Cuts at: 1022 1578 1022
Size: 556 5697
BsmBI CGTCTCn'nnnn_
Cuts at: 2277 2884 2277
Size: 607 5646
Bsp1286I G_dGCh'C
Cuts at: 704 818 881 1008 1110 1437 1484 1589 1613
Size: 114 63 127 102 327 47 105 24
Cuts at: 1613 1634 1668 1814 2248 2471 2510 2831 3134
Size: 21 34 146 434 223 39 321 303
Cuts at: 3134 3265 3876 3969 4043 4233 4295 5027 704
Size: 131 611 93 74 190 62 732 1930
Fragments arranged by size:
1930 732 611 434 327 321 303 223
190 146 131 127 114 105 102 93
74 63 62 47 39 34 24 21
BspEI T'CCGG_A
Cuts at: 5473 5473
Size: 6253
BspHI T'CATG_A
Cuts at: 5429 5429
Size: 6253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 665 1142 1797 2885 3301 3515 3817 4198 665
Size: 477 655 1088 416 214 302 381 2720
Fragments arranged by size:
2720 1088 655 477 416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1258 2519 2705 3050 4543 4597 6083 6248
Size: 1208 1261 186 345 1493 54 1486 165
Cuts at: 6248 50
Size: 55
Fragments arranged by size:
1493 1486 1261 1208 345 186 165 55
54
BsrDI GCAATG_nn'
Cuts at: 398 3024 4163 398
Size: 2626 1139 2488
Fragments arranged by size:
2626 2488 1139
BsrFI r'CCGG_y
Cuts at: 1146 2374 2423 4249 4430 4641 1146
Size: 1228 49 1826 181 211 2758
Fragments arranged by size:
2758 1826 1228 211 181 49
BssSI C'ACGA_G
Cuts at: 832 4522 4882 832
Size: 3690 360 2203
Fragments arranged by size:
3690 2203 360
BstAPI GCAn_nnn'nTGC
Cuts at: 438 3628 438
Size: 3190 3063
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6253
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1627 132
Size: 1495 4758
BstYI r'GATC_y
Cuts at: 1 1557 3276 3743 3766 4101 4347 5361 5447
Size: 1556 1719 467 23 335 246 1014 86
Cuts at: 5447 5459 5805 1
Size: 12 346 449
Fragments arranged by size:
1719 1556 1014 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 544 887 2009 2240 544
Size: 343 1122 231 4557
Fragments arranged by size:
4557 1122 343 231
BtgI C'CryG_G
Cuts at: 125 422 847 1227 1308 1410 2672 3197 125
Size: 297 425 380 81 102 1262 525 3181
Fragments arranged by size:
3181 1262 525 425 380 297 102 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1115 2025 2628 4201 4382 4545 1115
Size: 910 603 1573 181 163 2823
Fragments arranged by size:
2823 1573 910 603 181 163
BtsI GCAGTG_nn'
Cuts at: 148 289 1859 2180 2764 3036 3494 148
Size: 141 1570 321 584 272 458 2907
Fragments arranged by size:
2907 1570 584 458 321 272 141
DraI TTT'AAA
Cuts at: 3250 3250
Size: 6253
DrdI GACnn_nn'nnGTC
Cuts at: 2938 3957 4817 2938
Size: 1019 860 4374
Fragments arranged by size:
4374 1019 860
EaeI y'GGCC_r
Cuts at: 635 858 2216 2224 2376 2773 3089 3836 4010
Size: 223 1358 8 152 397 316 747 174
Cuts at: 4010 4401 4428 5775 635
Size: 391 27 1347 1113
Fragments arranged by size:
1358 1347 1113 747 397 391 316 223
174 152 27 8
EagI C'GGCC_G
Cuts at: 3836 3836
Size: 6253
EarI CTCTTCn'nnn_
Cuts at: 1502 1712 4274 4484 1502
Size: 210 2562 210 3271
Fragments arranged by size:
3271 2562 210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1300 2029 2101 2479 2581 4459 4771 4917 6063
Size: 729 72 378 102 1878 312 146 1146
Cuts at: 6063 1300
Size: 1490
Fragments arranged by size:
1878 1490 1146 729 378 312 146 102
72
EcoICRI GAG'CTC
Cuts at: 879 1611 3263 879
Size: 732 1652 3869
Fragments arranged by size:
3869 1652 732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 688 850 1003 1147 1195 1295 1451 1487
Size: 113 162 153 144 48 100 156 36
Cuts at: 1487 1957 2155 2431 2449 2581 2890 3148 4076
Size: 470 198 276 18 132 309 258 928
Cuts at: 4076 4508 5257 5525 575
Size: 432 749 268 1303
Fragments arranged by size:
1303 928 749 470 432 309 276 268
258 198 162 156 153 144 132 113
100 48 36 18
EcoNI CCTnn'n_nnAGG
Cuts at: 210 316 543 679 1126 1402 1446 1804 2560
Size: 106 227 136 447 276 44 358 756
Cuts at: 2560 2952 210
Size: 392 3511
Fragments arranged by size:
3511 756 447 392 358 276 227 136
106 44
EcoO109I rG'GnC_Cy
Cuts at: 324 575 672 684 710 815 837 953 1131
Size: 251 97 12 26 105 22 116 178
Cuts at: 1131 1161 1395 1451 1631 1674 2244 2553 2957
Size: 30 234 56 180 43 570 309 404
Cuts at: 2957 3228 3390 324
Size: 271 162 3187
Fragments arranged by size:
3187 570 404 309 271 251 234 180
178 162 116 105 97 56 43 30
26 22 12
EcoRI G'AATT_C
Cuts at: 3255 3255
Size: 6253
EcoRV GAT'ATC
Cuts at: 3114 3114
Size: 6253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4270 4270
Size: 6253
FspI TGC'GCA
Cuts at: 4032 4032
Size: 6253
HaeII r_GCGC'y
Cuts at: 438 1874 2327 2449 3685 3933 4957 5936 438
Size: 1436 453 122 1236 248 1024 979 755
Fragments arranged by size:
1436 1236 1024 979 755 453 248 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 597 629 661 693 742 774 792 824 1399
Size: 32 32 32 49 32 18 32 575
Cuts at: 1399 1431 2770 2802 2850 2882 4093 4123 4125
Size: 32 1339 32 48 32 1211 30 2
Cuts at: 4125 4155 597
Size: 30 2695
Fragments arranged by size:
2695 1339 1211 575 49 48 32 32
32 32 32 32 32 32 30 30
18 2
HincII GTy'rAC
Cuts at: 1920 3290 1920
Size: 1370 4883
HindIII A'AGCT_T
Cuts at: 457 457
Size: 6253
KasI G'GCGC_C
Cuts at: 3681 3929 3681
Size: 248 6005
KpnI G_GTAC'C
Cuts at: 3271 3271
Size: 6253
MfeI C'AATT_G
Cuts at: 1693 1693
Size: 6253
MluI A'CGCG_T
Cuts at: 5686 5686
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 672 2381 4924 5108 672
Size: 1709 2543 184 1817
Fragments arranged by size:
2543 1817 1709 184
MscI TGG'CCA
Cuts at: 637 2218 3091 4012 637
Size: 1581 873 921 2878
Fragments arranged by size:
2878 1581 921 873
MslI CAynn'nnrTG
Cuts at: 130 2804 3029 4240 4367 130
Size: 2674 225 1211 127 2016
Fragments arranged by size:
2674 2016 1211 225 127
MspA1I CmG'CkG
Cuts at: 739 1597 1660 2661 3362 3676 4036 5051 5296
Size: 858 63 1001 701 314 360 1015 245
Cuts at: 5296 739
Size: 1696
Fragments arranged by size:
1696 1015 1001 858 701 360 314 245
63
NaeI GCC'GGC
Cuts at: 1148 4432 1148
Size: 3284 2969
NarI GG'CG_CC
Cuts at: 3682 3930 3682
Size: 248 6005
NcoI C'CATG_G
Cuts at: 125 1227 125
Size: 1102 5151
NgoMIV G'CCGG_C
Cuts at: 1146 4430 1146
Size: 3284 2969
NheI G'CTAG_C
Cuts at: 2602 2602
Size: 6253
NruI TCG'CGA
Cuts at: 4614 4614
Size: 6253
NspI r_CATG'y
Cuts at: 3304 3804 4713 3304
Size: 500 909 4844
Fragments arranged by size:
4844 909 500
PasI CC'CwG_GG
Cuts at: 212 1249 1263 2652 212
Size: 1037 14 1389 3813
Fragments arranged by size:
3813 1389 1037 14
PciI A'CATG_T
Cuts at: 4709 4709
Size: 6253
PflMI CCAn_nnn'nTGG
Cuts at: 921 2214 921
Size: 1293 4960
PfoI T'CCnGG_A
Cuts at: 665 2963 665
Size: 2298 3955
PmeI GTTT'AAAC
Cuts at: 3250 3250
Size: 6253
PmlI CAC'GTG
Cuts at: 479 479
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2838 5392 5424 2806
Size: 32 2554 32 3635
Fragments arranged by size:
3635 2554 32 32
PpuMI rG'GwC_Cy
Cuts at: 710 837 1131 2553 2957 710
Size: 127 294 1422 404 4006
Fragments arranged by size:
4006 1422 404 294 127
PspOMI G'GGCC_C
Cuts at: 814 1630 2244 814
Size: 816 614 4823
Fragments arranged by size:
4823 816 614
PspXI vC'TCGA_Gb
Cuts at: 316 1238 316
Size: 922 5331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2623 2655 2623
Size: 32 6221
PstI C_TGCA'G
Cuts at: 434 1139 1406 1808 2882 3298 3983 434
Size: 705 267 402 1074 416 685 2704
Fragments arranged by size:
2704 1074 705 685 416 402 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6253
PvuII CAG'CTG
Cuts at: 3676 4036 3676
Size: 360 5893
RsrII CG'GwC_CG
Cuts at: 4446 4446
Size: 6253
SacI G_AGCT'C
Cuts at: 881 1613 3265 881
Size: 732 1652 3869
Fragments arranged by size:
3869 1652 732
SalI G'TCGA_C
Cuts at: 3288 3288
Size: 6253
SapI GCTCTTCn'nnn_
Cuts at: 4274 4484 4274
Size: 210 6043
SbfI CC_TGCA'GG
Cuts at: 1406 1808 3298 1406
Size: 402 1490 4361
Fragments arranged by size:
4361 1490 402
ScaI AGT'ACT
Cuts at: 5770 5770
Size: 6253
SexAI A'CCwGG_T
Cuts at: 2816 2816
Size: 6253
SfcI C'TryA_G
Cuts at: 33 430 1135 1402 1804 2878 2994 3294 3979
Size: 397 705 267 402 1074 116 300 685
Cuts at: 3979 4974 5165 33
Size: 995 191 1121
Fragments arranged by size:
1121 1074 995 705 685 402 397 300
267 191 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 1169 1169
Size: 6253
SfoI GGC'GCC
Cuts at: 3683 3931 3683
Size: 248 6005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6253
SmaI CCC'GGG
Cuts at: 813 969 1590 2551 3273 813
Size: 156 621 961 722 3793
Fragments arranged by size:
3793 961 722 621 156
SmlI C'TyrA_G
Cuts at: 316 1011 1238 1993 2952 3135 3223 3406 4321
Size: 695 227 755 959 183 88 183 915
Cuts at: 4321 4815 5077 316
Size: 494 262 1492
Fragments arranged by size:
1492 959 915 755 695 494 262 227
183 183 88
SnaBI TAC'GTA
Cuts at: 3243 3243
Size: 6253
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6253
SphI G_CATG'C
Cuts at: 3304 3804 3304
Size: 500 5753
SspI AAT'ATT
Cuts at: 145 145
Size: 6253
StuI AGG'CCT
Cuts at: 346 1026 346
Size: 680 5573
StyI C'CwwG_G
Cuts at: 125 1227 1295 3385 3739 125
Size: 1102 68 2090 354 2639
Fragments arranged by size:
2639 2090 1102 354 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2249 2665 2948 3834 3967 4426 4637 2249
Size: 416 283 886 133 459 211 3865
Fragments arranged by size:
3865 886 459 416 283 211 133
TatI w'GTAC_w
Cuts at: 5768 5768
Size: 6253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 1838 1870 2422 2434 2454 2466 5770
Size: 32 1544 32 552 12 20 12 3304
Cuts at: 5770 5802 262
Size: 32 713
Fragments arranged by size:
3304 1544 713 552 32 32 32 20
12 12
Tth111I GACn'n_nGTC
Cuts at: 4048 4048
Size: 6253
XbaI T'CTAG_A
Cuts at: 3282 3282
Size: 6253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1029 1560 1899 3746 1029
Size: 531 339 1847 3536
Fragments arranged by size:
3536 1847 531 339
XhoI C'TCGA_G
Cuts at: 316 1238 316
Size: 922 5331
XmaI C'CCGG_G
Cuts at: 811 967 1588 2549 3271 811
Size: 156 621 961 722 3793
Fragments arranged by size:
3793 961 722 621 156
XmnI GAAnn'nnTTC
Cuts at: 187 187
Size: 6253
ZraI GAC'GTC
Cuts at: 1791 1791
Size: 6253
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AlfI AscI AvrII BaeI BfrBI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
FspAI HpaI NdeI NotI NsiI PacI PshAI PsiI
SacII SanDI SgrAI SrfI SwaI