Restriction map for
pF1KSDA0390
(Circular) (Six-base) MAPSORT of: pF1KSDA0390.seq Check: 7719 from: 1 to: 7037
pF1KSDA0390 7037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 662 662
Size: 7037
AccI GT'mk_AC
Cuts at: 4075 4075
Size: 7037
Acc65I G'GTAC_C
Cuts at: 1412 4053 1412
Size: 2641 4396
AclI AA'CG_TT
Cuts at: 6877 6877
Size: 7037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 2658 3569 4862 5294 6041 6309 817
Size: 1841 911 1293 432 747 268 1545
Fragments arranged by size:
1841 1545 1293 911 747 432 268
AfeI AGC'GCT
Cuts at: 538 1520 2342 538
Size: 982 822 5233
Fragments arranged by size:
5233 982 822
AflII C'TTAA_G
Cuts at: 2422 2422
Size: 7037
AflIII A'CryG_T
Cuts at: 1823 4389 5493 6470 1823
Size: 2566 1104 977 2390
Fragments arranged by size:
2566 2390 1104 977
AgeI A'CCGG_T
Cuts at: 5425 5425
Size: 7037
AhdI GACnn_n'nnGTC
Cuts at: 2160 2160
Size: 7037
AleI CACnn'nnGTG
Cuts at: 188 1885 2075 2447 188
Size: 1697 190 372 4778
Fragments arranged by size:
4778 1697 372 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 793 1667 793
Size: 874 6163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1267 1299 1267
Size: 32 7005
AlwNI CAG_nnn'CTG
Cuts at: 231 456 3512 3786 3897 4342 5909 231
Size: 225 3056 274 111 445 1567 1359
Fragments arranged by size:
3056 1567 1359 445 274 225 111
ApaI G_GGCC'C
Cuts at: 1126 3890 1126
Size: 2764 4273
ApaLI G'TGCA_C
Cuts at: 1029 2068 2428 5807 1029
Size: 1039 360 3379 2259
Fragments arranged by size:
3379 2259 1039 360
ApoI r'AATT_y
Cuts at: 2862 3987 4041 6390 6646 2862
Size: 1125 54 2349 256 3253
Fragments arranged by size:
3253 2349 1125 256 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 7037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
AvaI C'yCGr_G
Cuts at: 268 292 1351 1972 2145 2733 3087 3260 4057
Size: 24 1059 621 173 588 354 173 797
Cuts at: 4057 268
Size: 3248
Fragments arranged by size:
3248 1059 797 621 588 354 173 173
24
BamHI G'GATC_C
Cuts at: 4062 4062
Size: 7037
BanI G'GyrC_C
Cuts at: 888 1356 1412 3763 4053 4467 4715 4750 888
Size: 468 56 2351 290 414 248 35 3175
Fragments arranged by size:
3175 2351 468 414 290 248 56 35
BanII G_rGCy'C
Cuts at: 182 275 1070 1126 1280 1472 2197 2368 3094
Size: 93 795 56 154 192 725 171 726
Cuts at: 3094 3170 3890 4051 5081 182
Size: 76 720 161 1030 2138
Fragments arranged by size:
2138 1030 795 726 725 720 192 171
161 154 93 76 56
BbeI G_GCGC'C
Cuts at: 4471 4719 4471
Size: 248 6789
BbsI GAAGACnn'nnnn_
Cuts at: 149 1778 2747 3829 149
Size: 1629 969 1082 3357
Fragments arranged by size:
3357 1629 1082 969
BbvCI CC'TCA_GC
Cuts at: 3715 3715
Size: 7037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3897 3931 3897
Size: 34 7003
BciVI GTATCCnnnnn_n'
Cuts at: 2432 4935 5696 7037 2432
Size: 2503 761 1341 2432
Fragments arranged by size:
2503 2432 1341 761
BclI T'GATC_A
Cuts at: 3329 4557 3329
Size: 1228 5809
BglI GCCn_nnn'nGGC
Cuts at: 820 829 853 937 1108 1130 3265 820
Size: 9 24 84 171 22 2135 4592
Fragments arranged by size:
4592 2135 171 84 24 22 9
BglII A'GATC_T
Cuts at: 1253 2317 4552 6589 1253
Size: 1064 2235 2037 1701
Fragments arranged by size:
2235 2037 1701 1064
BlpI GC'TnA_GC
Cuts at: 176 253 3951 4149 176
Size: 77 3698 198 3064
Fragments arranged by size:
3698 3064 198 77
Bme1580I G_kGCm'C
Cuts at: 338 523 607 893 1033 1126 1367 2026 2072
Size: 185 84 286 140 93 241 659 46
Cuts at: 2072 2256 2432 3890 4662 4755 5811 338
Size: 184 176 1458 772 93 1056 1564
Fragments arranged by size:
1564 1458 1056 772 659 286 241 185
184 176 140 93 93 84 46
BmgBI CAC'GTC
Cuts at: 876 876
Size: 7037
BmrI ACTGGGnnnn_n'
Cuts at: 2160 2996 4249 4330 4665 6809 2160
Size: 836 1253 81 335 2144 2388
Fragments arranged by size:
2388 2144 1253 836 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2784 5404 2251
Size: 533 2620 3884
Fragments arranged by size:
3884 2620 533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 751 1392 1530 2744 3169 3489 337
Size: 414 641 138 1214 425 320 3885
Fragments arranged by size:
3885 1214 641 425 414 320 138
Bpu10I CC'TnA_GC
Cuts at: 1462 3495 3715 1462
Size: 2033 220 4784
Fragments arranged by size:
4784 2033 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1189 1273 3361 4213 5092 5584 5882 628
Size: 561 84 2088 852 879 492 298 1783
Fragments arranged by size:
2088 1783 879 852 561 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 1895 2662 3795 6741 1895
Size: 767 1133 2946 2191
Fragments arranged by size:
2946 2191 1133 767
BsaAI yAC'GTr
Cuts at: 333 1080 1824 2121 3423 4029 4390 333
Size: 747 744 297 1302 606 361 2980
Fragments arranged by size:
2980 1302 747 744 606 361 297
BsaBI GATnn'nnATC
Cuts at: 6 4551 6588 6
Size: 4545 2037 455
Fragments arranged by size:
4545 2037 455
BsaHI Gr'CG_yC
Cuts at: 494 1562 2327 4468 4716 5418 6914 494
Size: 1068 765 2141 248 702 1496 617
Fragments arranged by size:
2141 1496 1068 765 702 617 248
BsaWI w'CCGG_w
Cuts at: 2405 3684 3810 4226 4747 5425 5699 5846 6257
Size: 1279 126 416 521 678 274 147 411
Cuts at: 6257 2405
Size: 3185
Fragments arranged by size:
3185 1279 678 521 416 411 274 147
126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1216 1246 1267 1297 1390 1420 1567 1597 1810
Size: 30 21 30 93 30 147 30 213
Cuts at: 1810 1840 2152 2182 3604 3622 3634 3652 3805
Size: 30 312 30 1422 18 12 18 153
Cuts at: 3805 3835 1216
Size: 30 4418
Fragments arranged by size:
4418 1422 312 213 153 147 93 30
30 30 30 30 30 30 21 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 334 721 748 1000 1075 1078 2119 2187
Size: 24 387 27 252 75 3 1041 68
Cuts at: 2187 2227 2268 2280 2320 2693 2709 3007 3084
Size: 40 41 12 40 373 16 298 77
Cuts at: 3084 3612 3630 310
Size: 528 18 3717
Fragments arranged by size:
3717 1041 528 387 373 298 252 77
75 68 41 40 40 27 24 18
16 12 3
BseYI C'CCAG_C
Cuts at: 248 387 669 815 1342 1471 1521 2311 2551
Size: 139 282 146 527 129 50 790 240
Cuts at: 2551 3770 4462 5220 5797 6293 248
Size: 1219 692 758 577 496 992
Fragments arranged by size:
1219 992 790 758 692 577 527 496
282 240 146 139 129 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 550 706 822 934 2101 3127 217
Size: 333 156 116 112 1167 1026 4127
Fragments arranged by size:
4127 1167 1026 333 156 116 112
BsiEI CG_ry'CG
Cuts at: 104 790 1103 1263 2037 4579 4625 5833 104
Size: 686 313 160 774 2542 46 1208 1308
Fragments arranged by size:
2542 1308 1208 774 686 313 160 46
BsiHKAI G_wGCw'C
Cuts at: 275 1033 1070 1244 2072 2432 3170 4051 4829
Size: 758 37 174 828 360 738 881 778
Cuts at: 4829 5019 5811 275
Size: 190 792 1501
Fragments arranged by size:
1501 881 828 792 778 758 738 360
190 174 37
Bsp1286I G_dGCh'C
Cuts at: 182 275 338 523 607 893 1033 1070 1126
Size: 93 63 185 84 286 140 37 56
Cuts at: 1126 1244 1280 1367 1472 2026 2072 2197 2256
Size: 118 36 87 105 554 46 125 59
Cuts at: 2256 2368 2432 3094 3170 3890 4051 4662 4755
Size: 112 64 662 76 720 161 611 93
Cuts at: 4755 4829 5019 5081 5811 182
Size: 74 190 62 730 1408
Fragments arranged by size:
1408 730 720 662 611 554 286 190
185 161 140 125 118 112 105 93
93 87 84 76 74 64 63 62
59 56 46 37 36
BspEI T'CCGG_A
Cuts at: 2405 6257 2405
Size: 3852 3185
BspHI T'CATG_A
Cuts at: 1439 6213 1439
Size: 4774 2263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 1913 1991 4087 4301 4603 4984 662
Size: 1251 78 2096 214 302 381 2715
Fragments arranged by size:
2715 2096 1251 381 302 214 78
BsrBI CCG'CTC
Cuts at: 50 344 1638 2136 2180 2219 2538 3404 3882
Size: 294 1294 498 44 39 319 866 478
Cuts at: 3882 5329 5383 6867 7032 50
Size: 1447 54 1484 165 55
Fragments arranged by size:
1484 1447 1294 866 498 478 319 294
165 55 54 44 39
BsrDI GCAATG_nn'
Cuts at: 3116 3614 4949 3116
Size: 498 1335 5204
Fragments arranged by size:
5204 1335 498
BsrFI r'CCGG_y
Cuts at: 1262 1456 2323 2485 3415 5035 5216 5425 1262
Size: 194 867 162 930 1620 181 209 2874
Fragments arranged by size:
2874 1620 930 867 209 194 181 162
BssHII G'CGCG_C
Cuts at: 738 738
Size: 7037
BssSI C'ACGA_G
Cuts at: 724 1606 3582 5308 5666 724
Size: 882 1976 1726 358 2095
Fragments arranged by size:
2095 1976 1726 882 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1037 1755 1857 4414 1037
Size: 718 102 2557 3660
Fragments arranged by size:
3660 2557 718 102
BstBI TT'CG_AA
Cuts at: 110 5398 110
Size: 5288 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 190 387 1483 1716 3198 190
Size: 197 1096 233 1482 4029
Fragments arranged by size:
4029 1482 1096 233 197
BstYI r'GATC_y
Cuts at: 1 1253 2317 4062 4529 4552 4887 5133 6145
Size: 1252 1064 1745 467 23 335 246 1012
Cuts at: 6145 6231 6243 6589 1
Size: 86 12 346 449
Fragments arranged by size:
1745 1252 1064 1012 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2434 2638 3928 2434
Size: 204 1290 5543
Fragments arranged by size:
5543 1290 204
BtgI C'CryG_G
Cuts at: 125 353 1367 1557 1721 2744 2973 3161 125
Size: 228 1014 190 164 1023 229 188 4001
Fragments arranged by size:
4001 1023 1014 229 228 190 188 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3749 4987 5168 5331 3749
Size: 1238 181 163 5455
Fragments arranged by size:
5455 1238 181 163
BtsI GCAGTG_nn'
Cuts at: 1242 2157 2704 3826 4280 1242
Size: 915 547 1122 454 3999
Fragments arranged by size:
3999 1122 915 547 454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 672 707 1116 1151 672
Size: 35 409 35 6558
Fragments arranged by size:
6558 409 35 35
DraI TTT'AAA
Cuts at: 4036 4036
Size: 7037
DraIII CAC_nnn'GTG
Cuts at: 888 1037 2021 2061 2979 888
Size: 149 984 40 918 4946
Fragments arranged by size:
4946 984 918 149 40
DrdI GACnn_nn'nnGTC
Cuts at: 2899 4743 5601 2899
Size: 1844 858 4335
Fragments arranged by size:
4335 1844 858
EaeI y'GGCC_r
Cuts at: 207 345 377 787 830 1100 1148 1260 1706
Size: 138 32 410 43 270 48 112 446
Cuts at: 1706 1718 2034 2369 3067 3417 4622 4796 5187
Size: 12 316 335 698 350 1205 174 391
Cuts at: 5187 5214 6559 207
Size: 27 1345 685
Fragments arranged by size:
1345 1205 698 685 446 410 391 350
335 316 270 174 138 112 48 43
32 27 12
EagI C'GGCC_G
Cuts at: 787 1100 1260 2034 4622 787
Size: 313 160 774 2588 3202
Fragments arranged by size:
3202 2588 774 313 160
EarI CTCTTCn'nnn_
Cuts at: 2823 3394 3545 3659 5060 5270 2823
Size: 571 151 114 1401 210 4590
Fragments arranged by size:
4590 1401 571 210 151 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 5245 5555 5701 6847 178
Size: 5067 310 146 1146 368
Fragments arranged by size:
5067 1146 368 310 146
EcoICRI GAG'CTC
Cuts at: 273 1068 3168 4049 273
Size: 795 2100 881 3261
Fragments arranged by size:
3261 2100 881 795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 751 817 1392 1530 2658 2744 3169 3489
Size: 414 66 575 138 1128 86 425 320
Cuts at: 3489 3569 4862 5294 6041 6309 337
Size: 80 1293 432 747 268 1065
Fragments arranged by size:
1293 1128 1065 747 575 432 425 414
320 268 138 86 80 66
EcoNI CCTnn'n_nnAGG
Cuts at: 682 3018 682
Size: 2336 4701
EcoO109I rG'GnC_Cy
Cuts at: 822 1122 1123 1990 2645 2683 2763 2873 3500
Size: 300 1 867 655 38 80 110 627
Cuts at: 3500 3852 3886 3887 3932 4176 822
Size: 352 34 1 45 244 3683
Fragments arranged by size:
3683 867 655 627 352 300 244 110
80 45 38 34 1 1
EcoRI G'AATT_C
Cuts at: 4041 4041
Size: 7037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2627 5056 2627
Size: 2429 4608
FspI TGC'GCA
Cuts at: 574 1219 4818 574
Size: 645 3599 2793
Fragments arranged by size:
3599 2793 645
FspAI rTGC'GCAy
Cuts at: 574 574
Size: 7037
HaeII r_GCGC'y
Cuts at: 373 540 1269 1522 2344 4471 4719 5741 6720
Size: 167 729 253 822 2127 248 1022 979
Cuts at: 6720 373
Size: 690
Fragments arranged by size:
2127 1022 979 822 729 690 253 248
167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1599 1933 1965 2146 2152 2178 2184 2400
Size: 32 334 32 181 6 26 6 216
Cuts at: 2400 2432 2584 2616 3064 3096 3321 3353 3592
Size: 32 152 32 448 32 225 32 239
Cuts at: 3592 3604 3622 3624 3636 3654 4879 4909 4911
Size: 12 18 2 12 18 1225 30 2
Cuts at: 4911 4941 1567
Size: 30 3663
Fragments arranged by size:
3663 1225 448 334 239 225 216 181
152 32 32 32 32 32 32 30
30 26 18 18 12 12 6 6
2 2
HincII GTy'rAC
Cuts at: 3243 4076 3243
Size: 833 6204
HindIII A'AGCT_T
Cuts at: 2609 2882 2609
Size: 273 6764
KasI G'GCGC_C
Cuts at: 4467 4715 4467
Size: 248 6789
KpnI G_GTAC'C
Cuts at: 1416 4057 1416
Size: 2641 4396
MluI A'CGCG_T
Cuts at: 6470 6470
Size: 7037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2205 5708 5892 2205
Size: 3503 184 3350
Fragments arranged by size:
3503 3350 184
MscI TGG'CCA
Cuts at: 379 1708 1720 4798 379
Size: 1329 12 3078 2618
Fragments arranged by size:
3078 2618 1329 12
MslI CAynn'nnrTG
Cuts at: 188 1720 1885 2075 2447 2591 2749 5026 5153
Size: 1532 165 190 372 144 158 2277 127
Cuts at: 5153 188
Size: 2072
Fragments arranged by size:
2277 2072 1532 372 190 165 158 144
127
MspA1I CmG'CkG
Cuts at: 387 762 828 950 1163 1617 1628 1755 2559
Size: 375 66 122 213 454 11 127 804
Cuts at: 2559 4148 4462 4822 5835 6080 387
Size: 1589 314 360 1013 245 1344
Fragments arranged by size:
1589 1344 1013 804 454 375 360 314
245 213 127 122 66 11
NaeI GCC'GGC
Cuts at: 1264 1458 2325 2487 5218 1264
Size: 194 867 162 2731 3083
Fragments arranged by size:
3083 2731 867 194 162
NarI GG'CG_CC
Cuts at: 4468 4716 4468
Size: 248 6789
NcoI C'CATG_G
Cuts at: 125 353 1367 1721 3161 125
Size: 228 1014 354 1440 4001
Fragments arranged by size:
4001 1440 1014 354 228
NgoMIV G'CCGG_C
Cuts at: 1262 1456 2323 2485 5216 1262
Size: 194 867 162 2731 3083
Fragments arranged by size:
3083 2731 867 194 162
NruI TCG'CGA
Cuts at: 2351 2351
Size: 7037
NspI r_CATG'y
Cuts at: 573 1218 1380 2754 3918 4090 4590 5497 573
Size: 645 162 1374 1164 172 500 907 2113
Fragments arranged by size:
2113 1374 1164 907 645 500 172 162
PasI CC'CwG_GG
Cuts at: 1113 1113
Size: 7037
PciI A'CATG_T
Cuts at: 5493 5493
Size: 7037
PflMI CCAn_nnn'nTGG
Cuts at: 354 669 2061 2979 3786 3897 354
Size: 315 1392 918 807 111 3494
Fragments arranged by size:
3494 1392 918 807 315 111
PfoI T'CCnGG_A
Cuts at: 1565 1565
Size: 7037
PmeI GTTT'AAAC
Cuts at: 4036 4036
Size: 7037
PmlI CAC'GTG
Cuts at: 333 1080 2121 3423 333
Size: 747 1041 1302 3947
Fragments arranged by size:
3947 1302 1041 747
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1267 1299 6176 6208 1267
Size: 32 4877 32 2096
Fragments arranged by size:
4877 2096 32 32
PpuMI rG'GwC_Cy
Cuts at: 1990 2763 2873 3852 1990
Size: 773 110 979 5175
Fragments arranged by size:
5175 979 773 110
PspOMI G'GGCC_C
Cuts at: 1122 3886 1122
Size: 2764 4273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 496 528 496
Size: 32 7005
PstI C_TGCA'G
Cuts at: 782 854 1430 1646 1760 1988 3755 4084 4769
Size: 72 576 216 114 228 1767 329 685
Cuts at: 4769 782
Size: 3050
Fragments arranged by size:
3050 1767 685 576 329 228 216 114
72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7037
PvuII CAG'CTG
Cuts at: 387 762 1628 4462 4822 387
Size: 375 866 2834 360 2602
Fragments arranged by size:
2834 2602 866 375 360
RsrII CG'GwC_CG
Cuts at: 5232 5232
Size: 7037
SacI G_AGCT'C
Cuts at: 275 1070 3170 4051 275
Size: 795 2100 881 3261
Fragments arranged by size:
3261 2100 881 795
SalI G'TCGA_C
Cuts at: 4074 4074
Size: 7037
SapI GCTCTTCn'nnn_
Cuts at: 2823 3394 5060 5270 2823
Size: 571 1666 210 4590
Fragments arranged by size:
4590 1666 571 210
SbfI CC_TGCA'GG
Cuts at: 854 1988 4084 854
Size: 1134 2096 3807
Fragments arranged by size:
3807 2096 1134
ScaI AGT'ACT
Cuts at: 6554 6554
Size: 7037
SexAI A'CCwGG_T
Cuts at: 2057 2057
Size: 7037
SfcI C'TryA_G
Cuts at: 33 778 850 1426 1642 1756 1984 3751 3936
Size: 745 72 576 216 114 228 1767 185
Cuts at: 3936 4080 4765 5758 5949 33
Size: 144 685 993 191 1121
Fragments arranged by size:
1767 1121 993 745 685 576 228 216
191 185 144 114 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 829 1108 829
Size: 279 6758
SfoI GGC'GCC
Cuts at: 4469 4717 4469
Size: 248 6789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7037
SmaI CCC'GGG
Cuts at: 3089 3262 4059 3089
Size: 173 797 6067
Fragments arranged by size:
6067 797 173
SmlI C'TyrA_G
Cuts at: 643 1204 1288 1972 2422 3376 4192 5107 5599
Size: 561 84 684 450 954 816 915 492
Cuts at: 5599 5861 643
Size: 262 1819
Fragments arranged by size:
1819 954 915 816 684 561 492 450
262 84
SnaBI TAC'GTA
Cuts at: 4029 4029
Size: 7037
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7037
SphI G_CATG'C
Cuts at: 4090 4590 4090
Size: 500 6537
StyI C'CwwG_G
Cuts at: 125 147 353 1367 1655 1721 2868 3161 3366
Size: 22 206 1014 288 66 1147 293 205
Cuts at: 3366 3644 3857 4171 4525 125
Size: 278 213 314 354 2637
Fragments arranged by size:
2637 1147 1014 354 314 293 288 278
213 206 205 66 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2824 3912 4620 4753 5212 5421 2824
Size: 1088 708 133 459 209 4440
Fragments arranged by size:
4440 1088 708 459 209 133
TatI w'GTAC_w
Cuts at: 1403 6552 1403
Size: 5149 1888
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1216 1248 2778 2810 3805 3837 6554 6586 1216
Size: 32 1530 32 995 32 2717 32 1667
Fragments arranged by size:
2717 1667 1530 995 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4834 4834
Size: 7037
XbaI T'CTAG_A
Cuts at: 3339 4068 3339
Size: 729 6308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1548 2980 3168 4532 1548
Size: 1432 188 1364 4053
Fragments arranged by size:
4053 1432 1364 188
XhoI C'TCGA_G
Cuts at: 1972 1972
Size: 7037
XmaI C'CCGG_G
Cuts at: 3087 3260 4057 3087
Size: 173 797 6067
Fragments arranged by size:
6067 797 173
XmnI GAAnn'nnTTC
Cuts at: 595 2401 595
Size: 1806 5231
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI AvrII BaeI BfrBI BmtI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I ClaI EcoRV FseI HpaI
MfeI NdeI NheI NotI NsiI PacI PshAI PsiI
PspXI SacII SanDI SgrAI SrfI SspI StuI SwaI
ZraI