Restriction map for
pF1KSDA0396
(Circular) (Six-base) MAPSORT of: pF1KSDA0396.seq Check: 8282 from: 1 to: 5018
pF1KSDA0396 5018 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 601 601
Size: 5018
AatII G_ACGT'C
Cuts at: 1643 1643
Size: 5018
AccI GT'mk_AC
Cuts at: 149 2056 149
Size: 1907 3111
Acc65I G'GTAC_C
Cuts at: 2034 2034
Size: 5018
AclI AA'CG_TT
Cuts at: 4858 4858
Size: 5018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 2843 3275 4022 4290 731
Size: 2112 432 747 268 1459
Fragments arranged by size:
2112 1459 747 432 268
AfeI AGC'GCT
Cuts at: 262 262
Size: 5018
AflIII A'CryG_T
Cuts at: 2370 3474 4451 2370
Size: 1104 977 2937
Fragments arranged by size:
2937 1104 977
AgeI A'CCGG_T
Cuts at: 203 3406 203
Size: 3203 1815
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 5018
AlwNI CAG_nnn'CTG
Cuts at: 1065 1417 2323 3890 1065
Size: 352 906 1567 2193
Fragments arranged by size:
2193 1567 906 352
ApaLI G'TGCA_C
Cuts at: 3788 3788
Size: 5018
ApoI r'AATT_y
Cuts at: 1201 2022 4371 4627 1201
Size: 821 2349 256 1592
Fragments arranged by size:
2349 1592 821 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5018
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
AvaI C'yCGr_G
Cuts at: 1931 2038 1931
Size: 107 4911
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1859 1892 1859
Size: 33 4985
BamHI G'GATC_C
Cuts at: 2043 2043
Size: 5018
BanI G'GyrC_C
Cuts at: 355 388 895 2034 2448 2696 2731 355
Size: 33 507 1139 414 248 35 2642
Fragments arranged by size:
2642 1139 507 414 248 35 33
BanII G_rGCy'C
Cuts at: 1818 2032 3062 1818
Size: 214 1030 3774
Fragments arranged by size:
3774 1030 214
BbeI G_GCGC'C
Cuts at: 2452 2700 2452
Size: 248 4770
BbvCI CC'TCA_GC
Cuts at: 1694 1694
Size: 5018
BciVI GTATCCnnnnn_n'
Cuts at: 2916 3677 5018 2916
Size: 761 1341 2916
Fragments arranged by size:
2916 1341 761
BclI T'GATC_A
Cuts at: 2538 2538
Size: 5018
BglI GCCn_nnn'nGGC
Cuts at: 140 140
Size: 5018
BglII A'GATC_T
Cuts at: 1535 2533 4570 1535
Size: 998 2037 1983
Fragments arranged by size:
2037 1983 998
BlpI GC'TnA_GC
Cuts at: 2130 2130
Size: 5018
Bme1580I G_kGCm'C
Cuts at: 722 1731 2643 2736 3792 722
Size: 1009 912 93 1056 1948
Fragments arranged by size:
1948 1056 1009 912 93
BmrI ACTGGGnnnn_n'
Cuts at: 1880 2230 2311 2646 4790 1880
Size: 350 81 335 2144 2108
Fragments arranged by size:
2144 2108 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 5018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1087 1598 434
Size: 653 511 3854
Fragments arranged by size:
3854 653 511
Bpu10I CC'TnA_GC
Cuts at: 1694 1694
Size: 5018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1277 1900 2194 3073 3565 3863 1277
Size: 623 294 879 492 298 2432
Fragments arranged by size:
2432 879 623 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 4722 4722
Size: 5018
BsaAI yAC'GTr
Cuts at: 2010 2371 2010
Size: 361 4657
BsaBI GATnn'nnATC
Cuts at: 6 2532 4569 6
Size: 2526 2037 455
Fragments arranged by size:
2526 2037 455
BsaHI Gr'CG_yC
Cuts at: 787 1640 2449 2697 3399 4895 787
Size: 853 809 248 702 1496 910
Fragments arranged by size:
1496 910 853 809 702 248
BsaWI w'CCGG_w
Cuts at: 203 2207 2728 3406 3680 3827 4238 203
Size: 2004 521 678 274 147 411 983
Fragments arranged by size:
2004 983 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 449 479 449
Size: 30 4988
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1190 1190
Size: 5018
BseYI C'CCAG_C
Cuts at: 715 2443 3201 3778 4274 715
Size: 1728 758 577 496 1459
Fragments arranged by size:
1728 1459 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 428 1222 1521 358
Size: 70 794 299 3855
Fragments arranged by size:
3855 794 299 70
BsiEI CG_ry'CG
Cuts at: 104 861 2560 2606 3814 104
Size: 757 1699 46 1208 1308
Fragments arranged by size:
1699 1308 1208 757 46
BsiHKAI G_wGCw'C
Cuts at: 1818 2032 2810 3000 3792 1818
Size: 214 778 190 792 3044
Fragments arranged by size:
3044 792 778 214 190
BsmI GAATG_Cn'
Cuts at: 1707 1707
Size: 5018
Bsp1286I G_dGCh'C
Cuts at: 722 1731 1818 2032 2643 2736 2810 3000 3062
Size: 1009 87 214 611 93 74 190 62
Cuts at: 3062 3792 722
Size: 730 1948
Fragments arranged by size:
1948 1009 730 611 214 190 93 87
74 62
BspEI T'CCGG_A
Cuts at: 4238 4238
Size: 5018
BspHI T'CATG_A
Cuts at: 4194 4194
Size: 5018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 601 2068 2282 2584 2965 601
Size: 1467 214 302 381 2654
Fragments arranged by size:
2654 1467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3310 3364 4848 5013 50
Size: 3260 54 1484 165 55
Fragments arranged by size:
3260 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 569 694 2930 297
Size: 272 125 2236 2385
Fragments arranged by size:
2385 2236 272 125
BsrFI r'CCGG_y
Cuts at: 203 3016 3197 3406 203
Size: 2813 181 209 1815
Fragments arranged by size:
2813 1815 209 181
BssSI C'ACGA_G
Cuts at: 1568 3289 3647 1568
Size: 1721 358 2939
Fragments arranged by size:
2939 1721 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2395 2395
Size: 5018
BstBI TT'CG_AA
Cuts at: 110 3379 110
Size: 3269 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1030 1030
Size: 5018
BstYI r'GATC_y
Cuts at: 1 1275 1535 1970 2043 2510 2533 2868 3114
Size: 1274 260 435 73 467 23 335 246
Cuts at: 3114 4126 4212 4224 4570 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1274 1012 467 449 435 346 335 260
246 86 73 23 12
BstZ17I GTA'TAC
Cuts at: 150 150
Size: 5018
Bsu36I CC'TnA_GG
Cuts at: 1058 1058
Size: 5018
BtgI C'CryG_G
Cuts at: 125 453 777 1706 125
Size: 328 324 929 3437
Fragments arranged by size:
3437 929 328 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 2968 3149 3312 264
Size: 2704 181 163 1970
Fragments arranged by size:
2704 1970 181 163
BtsI GCAGTG_nn'
Cuts at: 392 1216 1371 2261 392
Size: 824 155 890 3149
Fragments arranged by size:
3149 890 824 155
DraI TTT'AAA
Cuts at: 2017 2017
Size: 5018
DraIII CAC_nnn'GTG
Cuts at: 682 682
Size: 5018
DrdI GACnn_nn'nnGTC
Cuts at: 376 2724 3582 376
Size: 2348 858 1812
Fragments arranged by size:
2348 1812 858
EaeI y'GGCC_r
Cuts at: 158 185 774 2603 2777 3168 3195 4540 158
Size: 27 589 1829 174 391 27 1345 636
Fragments arranged by size:
1829 1345 636 589 391 174 27 27
EagI C'GGCC_G
Cuts at: 2603 2603
Size: 5018
EarI CTCTTCn'nnn_
Cuts at: 1100 1982 3041 3251 1100
Size: 882 1059 210 2867
Fragments arranged by size:
2867 1059 882 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3226 3536 3682 4828 3226
Size: 310 146 1146 3416
Fragments arranged by size:
3416 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1816 2030 1816
Size: 214 4804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 731 1087 1598 2843 3275 4022 4290 434
Size: 297 356 511 1245 432 747 268 1162
Fragments arranged by size:
1245 1162 747 511 432 356 297 268
EcoO109I rG'GnC_Cy
Cuts at: 132 1335 2157 132
Size: 1203 822 2993
Fragments arranged by size:
2993 1203 822
EcoRI G'AATT_C
Cuts at: 2022 2022
Size: 5018
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1027 3037 1027
Size: 2010 3008
FspI TGC'GCA
Cuts at: 2799 2799
Size: 5018
HaeII r_GCGC'y
Cuts at: 264 2452 2700 3722 4701 264
Size: 2188 248 1022 979 581
Fragments arranged by size:
2188 1022 979 581 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2860 2890 2892 2922 2860
Size: 30 2 30 4956
Fragments arranged by size:
4956 30 30 2
HincII GTy'rAC
Cuts at: 1602 2057 1602
Size: 455 4563
HindIII A'AGCT_T
Cuts at: 878 878
Size: 5018
KasI G'GCGC_C
Cuts at: 2448 2696 2448
Size: 248 4770
KpnI G_GTAC'C
Cuts at: 2038 2038
Size: 5018
MluI A'CGCG_T
Cuts at: 4451 4451
Size: 5018
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 850 1977 3689 3873 850
Size: 1127 1712 184 1995
Fragments arranged by size:
1995 1712 1127 184
MscI TGG'CCA
Cuts at: 776 2779 776
Size: 2003 3015
MslI CAynn'nnrTG
Cuts at: 626 628 1028 1319 1634 1825 3007 3134 626
Size: 2 400 291 315 191 1182 127 2510
Fragments arranged by size:
2510 1182 400 315 291 191 127 2
MspA1I CmG'CkG
Cuts at: 412 709 2129 2443 2803 3816 4061 412
Size: 297 1420 314 360 1013 245 1369
Fragments arranged by size:
1420 1369 1013 360 314 297 245
NaeI GCC'GGC
Cuts at: 3199 3199
Size: 5018
NarI GG'CG_CC
Cuts at: 2449 2697 2449
Size: 248 4770
NcoI C'CATG_G
Cuts at: 125 453 777 125
Size: 328 324 4366
Fragments arranged by size:
4366 328 324
NdeI CA'TA_TG
Cuts at: 1100 1100
Size: 5018
NgoMIV G'CCGG_C
Cuts at: 3197 3197
Size: 5018
NspI r_CATG'y
Cuts at: 512 2071 2571 3478 512
Size: 1559 500 907 2052
Fragments arranged by size:
2052 1559 907 500
PciI A'CATG_T
Cuts at: 3474 3474
Size: 5018
PmeI GTTT'AAAC
Cuts at: 2017 2017
Size: 5018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 824 856 4157 4189 824
Size: 32 3301 32 1653
Fragments arranged by size:
3301 1653 32 32
PstI C_TGCA'G
Cuts at: 1648 2065 2750 1648
Size: 417 685 3916
Fragments arranged by size:
3916 685 417
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5018
PvuII CAG'CTG
Cuts at: 412 709 2443 2803 412
Size: 297 1734 360 2627
Fragments arranged by size:
2627 1734 360 297
RsrII CG'GwC_CG
Cuts at: 3213 3213
Size: 5018
SacI G_AGCT'C
Cuts at: 1818 2032 1818
Size: 214 4804
SalI G'TCGA_C
Cuts at: 2055 2055
Size: 5018
SapI GCTCTTCn'nnn_
Cuts at: 1100 3041 3251 1100
Size: 1941 210 2867
Fragments arranged by size:
2867 1941 210
SbfI CC_TGCA'GG
Cuts at: 2065 2065
Size: 5018
ScaI AGT'ACT
Cuts at: 4535 4535
Size: 5018
SfcI C'TryA_G
Cuts at: 33 761 1644 2061 2746 3739 3930 33
Size: 728 883 417 685 993 191 1121
Fragments arranged by size:
1121 993 883 728 685 417 191
SfoI GGC'GCC
Cuts at: 2450 2698 2450
Size: 248 4770
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5018
SmaI CCC'GGG
Cuts at: 2040 2040
Size: 5018
SmlI C'TyrA_G
Cuts at: 1292 1915 2173 3088 3580 3842 1292
Size: 623 258 915 492 262 2468
Fragments arranged by size:
2468 915 623 492 262 258
SnaBI TAC'GTA
Cuts at: 2010 2010
Size: 5018
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5018
SphI G_CATG'C
Cuts at: 512 2071 2571 512
Size: 1559 500 2959
Fragments arranged by size:
2959 1559 500
SspI AAT'ATT
Cuts at: 1146 1407 1146
Size: 261 4757
StuI AGG'CCT
Cuts at: 1109 1109
Size: 5018
StyI C'CwwG_G
Cuts at: 125 453 777 2152 2506 125
Size: 328 324 1375 354 2637
Fragments arranged by size:
2637 1375 354 328 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 875 883 1282 2601 2734 3193 3402 875
Size: 8 399 1319 133 459 209 2491
Fragments arranged by size:
2491 1319 459 399 209 133 8
TatI w'GTAC_w
Cuts at: 1748 1864 4533 1748
Size: 116 2669 2233
Fragments arranged by size:
2669 2233 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 449 481 1119 1151 4535 4567 449
Size: 32 638 32 3384 32 900
Fragments arranged by size:
3384 900 638 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2815 2815
Size: 5018
XbaI T'CTAG_A
Cuts at: 2049 2049
Size: 5018
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 2513 132
Size: 2381 2637
XmaI C'CCGG_G
Cuts at: 2038 2038
Size: 5018
ZraI GAC'GTC
Cuts at: 1641 1641
Size: 5018
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AloI ApaI AscI AvrII BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI XhoI
XmnI