Restriction map for
pF1KSDA0398
(Circular) (Six-base) MAPSORT of: pF1KSDA0398.seq Check: 9081 from: 1 to: 4567
pF1KSDA0398 4567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 284 1295 1603 284
Size: 1011 308 3248
Fragments arranged by size:
3248 1011 308
Acc65I G'GTAC_C
Cuts at: 1581 1581
Size: 4567
AclI AA'CG_TT
Cuts at: 4407 4407
Size: 4567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 577 1100 2390 2822 3571 3839 440
Size: 137 523 1290 432 749 268 1168
Fragments arranged by size:
1290 1168 749 523 432 268 137
AflII C'TTAA_G
Cuts at: 1491 1491
Size: 4567
AflIII A'CryG_T
Cuts at: 1917 3023 4000 1917
Size: 1106 977 2484
Fragments arranged by size:
2484 1106 977
AgeI A'CCGG_T
Cuts at: 2955 2955
Size: 4567
AleI CACnn'nnGTG
Cuts at: 951 951
Size: 4567
AlwNI CAG_nnn'CTG
Cuts at: 241 574 1870 3439 241
Size: 333 1296 1569 1369
Fragments arranged by size:
1569 1369 1296 333
ApaLI G'TGCA_C
Cuts at: 3337 3337
Size: 4567
ApoI r'AATT_y
Cuts at: 132 192 682 892 934 1156 1234 1315 1569
Size: 60 490 210 42 222 78 81 254
Cuts at: 1569 3920 4176 132
Size: 2351 256 523
Fragments arranged by size:
2351 523 490 256 254 222 210 81
78 60 42
AseI AT'TA_AT
Cuts at: 20 221 20
Size: 201 4366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4567
AvaI C'yCGr_G
Cuts at: 1585 1585
Size: 4567
BamHI G'GATC_C
Cuts at: 1590 1590
Size: 4567
BanI G'GyrC_C
Cuts at: 1581 1995 2243 2278 1581
Size: 414 248 35 3870
Fragments arranged by size:
3870 414 248 35
BanII G_rGCy'C
Cuts at: 1579 2609 1579
Size: 1030 3537
BbeI G_GCGC'C
Cuts at: 1999 2247 1999
Size: 248 4319
BbsI GAAGACnn'nnnn_
Cuts at: 200 200
Size: 4567
BciVI GTATCCnnnnn_n'
Cuts at: 2463 3226 4567 2463
Size: 763 1341 2463
Fragments arranged by size:
2463 1341 763
BclI T'GATC_A
Cuts at: 2085 2085
Size: 4567
BglI GCCn_nnn'nGGC
Cuts at: 1058 1058
Size: 4567
BglII A'GATC_T
Cuts at: 2080 4119 2080
Size: 2039 2528
BlpI GC'TnA_GC
Cuts at: 1677 1677
Size: 4567
Bme1580I G_kGCm'C
Cuts at: 2190 2283 3341 2190
Size: 93 1058 3416
Fragments arranged by size:
3416 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 257 1777 1858 2193 4339 257
Size: 1520 81 335 2146 485
Fragments arranged by size:
2146 1520 485 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 4567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 503
Size: 4567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1741 2620 3114 3412 538
Size: 1203 879 494 298 1693
Fragments arranged by size:
1693 1203 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4271 4271
Size: 4567
BsaAI yAC'GTr
Cuts at: 1557 1918 1557
Size: 361 4206
BsaBI GATnn'nnATC
Cuts at: 6 2079 4118 6
Size: 2073 2039 455
Fragments arranged by size:
2073 2039 455
BsaHI Gr'CG_yC
Cuts at: 1104 1996 2244 2948 4444 1104
Size: 892 248 704 1496 1227
Fragments arranged by size:
1496 1227 892 704 248
BsaWI w'CCGG_w
Cuts at: 1754 2275 2955 3229 3376 3787 1754
Size: 521 680 274 147 411 2534
Fragments arranged by size:
2534 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1990 2748 3327 3823 1990
Size: 758 579 496 2734
Fragments arranged by size:
2734 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 908
Size: 4567
BsiEI CG_ry'CG
Cuts at: 104 2107 2153 3363 104
Size: 2003 46 1210 1308
Fragments arranged by size:
2003 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1579 2357 2547 3341 1579
Size: 778 190 794 2805
Fragments arranged by size:
2805 794 778 190
BsmI GAATG_Cn'
Cuts at: 1373 1373
Size: 4567
Bsp1286I G_dGCh'C
Cuts at: 1579 2190 2283 2357 2547 2609 3341 1579
Size: 611 93 74 190 62 732 2805
Fragments arranged by size:
2805 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3787 3787
Size: 4567
BspHI T'CATG_A
Cuts at: 3743 3743
Size: 4567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1615 1829 2131 2512 1615
Size: 214 302 381 3670
Fragments arranged by size:
3670 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2857 2911 4397 4562 50
Size: 2807 54 1486 165 55
Fragments arranged by size:
2807 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1252 2477 1252
Size: 1225 3342
BsrFI r'CCGG_y
Cuts at: 2563 2744 2955 2563
Size: 181 211 4175
Fragments arranged by size:
4175 211 181
BssSI C'ACGA_G
Cuts at: 2836 3196 2836
Size: 360 4207
BstAPI GCAn_nnn'nTGC
Cuts at: 136 1942 136
Size: 1806 2761
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4567
BstEII G'GTnAC_C
Cuts at: 1475 1475
Size: 4567
BstYI r'GATC_y
Cuts at: 1 1590 2057 2080 2415 2661 3675 3761 3773
Size: 1589 467 23 335 246 1014 86 12
Cuts at: 3773 4119 1
Size: 346 449
Fragments arranged by size:
1589 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2515 2696 2859 2515
Size: 181 163 4223
Fragments arranged by size:
4223 181 163
BtsI GCAGTG_nn'
Cuts at: 1808 1808
Size: 4567
DraI TTT'AAA
Cuts at: 1564 1564
Size: 4567
DrdI GACnn_nn'nnGTC
Cuts at: 2271 3131 2271
Size: 860 3707
EaeI y'GGCC_r
Cuts at: 2150 2324 2715 2742 4089 2150
Size: 174 391 27 1347 2628
Fragments arranged by size:
2628 1347 391 174 27
EagI C'GGCC_G
Cuts at: 2150 2150
Size: 4567
EarI CTCTTCn'nnn_
Cuts at: 361 2588 2798 361
Size: 2227 210 2130
Fragments arranged by size:
2227 2130 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2773 3085 3231 4377 2773
Size: 312 146 1146 2963
Fragments arranged by size:
2963 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1577 1577
Size: 4567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 503 577 1100 2390 2822 3571 3839 440
Size: 63 74 523 1290 432 749 268 1168
Fragments arranged by size:
1290 1168 749 523 432 268 74 63
EcoO109I rG'GnC_Cy
Cuts at: 1704 1704
Size: 4567
EcoRI G'AATT_C
Cuts at: 192 1234 1315 1569 192
Size: 1042 81 254 3190
Fragments arranged by size:
3190 1042 254 81
EcoRV GAT'ATC
Cuts at: 720 720
Size: 4567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 337 1496 2584 337
Size: 1159 1088 2320
Fragments arranged by size:
2320 1159 1088
FspI TGC'GCA
Cuts at: 2346 2346
Size: 4567
HaeII r_GCGC'y
Cuts at: 1999 2247 3271 4250 1999
Size: 248 1024 979 2316
Fragments arranged by size:
2316 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 2407 2437 2439 2469 343
Size: 32 2032 30 2 30 2441
Fragments arranged by size:
2441 2032 32 30 30 2
HincII GTy'rAC
Cuts at: 1604 1604
Size: 4567
HindIII A'AGCT_T
Cuts at: 1112 1112
Size: 4567
KasI G'GCGC_C
Cuts at: 1995 2243 1995
Size: 248 4319
KpnI G_GTAC'C
Cuts at: 1585 1585
Size: 4567
MluI A'CGCG_T
Cuts at: 4000 4000
Size: 4567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3238 3422 3238
Size: 184 4383
MscI TGG'CCA
Cuts at: 2326 2326
Size: 4567
MslI CAynn'nnrTG
Cuts at: 584 951 2554 2681 584
Size: 367 1603 127 2470
Fragments arranged by size:
2470 1603 367 127
MspA1I CmG'CkG
Cuts at: 840 1676 1990 2350 3365 3610 840
Size: 836 314 360 1015 245 1797
Fragments arranged by size:
1797 1015 836 360 314 245
NaeI GCC'GGC
Cuts at: 2746 2746
Size: 4567
NarI GG'CG_CC
Cuts at: 1996 2244 1996
Size: 248 4319
NcoI C'CATG_G
Cuts at: 125 125
Size: 4567
NgoMIV G'CCGG_C
Cuts at: 2744 2744
Size: 4567
NruI TCG'CGA
Cuts at: 2928 2928
Size: 4567
NspI r_CATG'y
Cuts at: 1444 1618 2118 3027 1444
Size: 174 500 909 2984
Fragments arranged by size:
2984 909 500 174
PasI CC'CwG_GG
Cuts at: 1054 1054
Size: 4567
PciI A'CATG_T
Cuts at: 3023 3023
Size: 4567
PmeI GTTT'AAAC
Cuts at: 1564 1564
Size: 4567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3706 3738 3706
Size: 32 4535
PshAI GACnn'nnGTC
Cuts at: 268 268
Size: 4567
PsiI TTA'TAA
Cuts at: 898 898
Size: 4567
PstI C_TGCA'G
Cuts at: 970 1612 2297 970
Size: 642 685 3240
Fragments arranged by size:
3240 685 642
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4567
PvuII CAG'CTG
Cuts at: 1990 2350 1990
Size: 360 4207
RsrII CG'GwC_CG
Cuts at: 2760 2760
Size: 4567
SacI G_AGCT'C
Cuts at: 1579 1579
Size: 4567
SalI G'TCGA_C
Cuts at: 1602 1602
Size: 4567
SapI GCTCTTCn'nnn_
Cuts at: 2588 2798 2588
Size: 210 4357
SbfI CC_TGCA'GG
Cuts at: 1612 1612
Size: 4567
ScaI AGT'ACT
Cuts at: 4084 4084
Size: 4567
SfcI C'TryA_G
Cuts at: 33 273 966 1608 2293 3288 3479 33
Size: 240 693 642 685 995 191 1121
Fragments arranged by size:
1121 995 693 685 642 240 191
SfoI GGC'GCC
Cuts at: 1997 2245 1997
Size: 248 4319
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4567
SmaI CCC'GGG
Cuts at: 1587 1587
Size: 4567
SmlI C'TyrA_G
Cuts at: 517 1491 1720 2635 3129 3391 517
Size: 974 229 915 494 262 1693
Fragments arranged by size:
1693 974 915 494 262 229
SnaBI TAC'GTA
Cuts at: 1557 1557
Size: 4567
SpeI A'CTAG_T
Cuts at: 67 1290 67
Size: 1223 3344
SphI G_CATG'C
Cuts at: 1618 2118 1618
Size: 500 4067
SspI AAT'ATT
Cuts at: 219 219
Size: 4567
StuI AGG'CCT
Cuts at: 1375 1375
Size: 4567
StyI C'CwwG_G
Cuts at: 125 1376 1699 2053 125
Size: 1251 323 354 2639
Fragments arranged by size:
2639 1251 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2148 2281 2740 2951 2148
Size: 133 459 211 3764
Fragments arranged by size:
3764 459 211 133
TatI w'GTAC_w
Cuts at: 799 1277 1451 4082 799
Size: 478 174 2631 1284
Fragments arranged by size:
2631 1284 478 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 651 683 4084 4116 651
Size: 32 3401 32 1102
Fragments arranged by size:
3401 1102 32 32
Tth111I GACn'n_nGTC
Cuts at: 2362 2362
Size: 4567
XbaI T'CTAG_A
Cuts at: 1596 1596
Size: 4567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2060 2060
Size: 4567
XmaI C'CCGG_G
Cuts at: 1585 1585
Size: 4567
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NsiI PacI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI XmnI ZraI