Restriction map for
pF1KSDA0410
(Circular) (Six-base) MAPSORT of: pF1KSDA0410.seq Check: 1261 from: 1 to: 4594
pF1KSDA0410 4594 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1630 1630
Size: 4594
Acc65I G'GTAC_C
Cuts at: 1608 1608
Size: 4594
AclI AA'CG_TT
Cuts at: 4434 4434
Size: 4594
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 871 1015 1146 1180 1318 2417 2849 3598
Size: 439 144 131 34 138 1099 432 749
Cuts at: 3598 3866 432
Size: 268 1160
Fragments arranged by size:
1160 1099 749 439 432 268 144 138
131 34
AflII C'TTAA_G
Cuts at: 967 967
Size: 4594
AflIII A'CryG_T
Cuts at: 1944 3050 4027 1944
Size: 1106 977 2511
Fragments arranged by size:
2511 1106 977
AgeI A'CCGG_T
Cuts at: 2982 2982
Size: 4594
AleI CACnn'nnGTG
Cuts at: 172 172
Size: 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1568 1568
Size: 4594
AlwNI CAG_nnn'CTG
Cuts at: 478 1005 1150 1897 3466 478
Size: 527 145 747 1569 1606
Fragments arranged by size:
1606 1569 747 527 145
ApaLI G'TGCA_C
Cuts at: 3364 3364
Size: 4594
ApoI r'AATT_y
Cuts at: 904 1298 1596 3947 4203 904
Size: 394 298 2351 256 1295
Fragments arranged by size:
2351 1295 394 298 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4594
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4594
AvaI C'yCGr_G
Cuts at: 1612 1612
Size: 4594
BamHI G'GATC_C
Cuts at: 1617 1617
Size: 4594
BanI G'GyrC_C
Cuts at: 1608 2022 2270 2305 1608
Size: 414 248 35 3897
Fragments arranged by size:
3897 414 248 35
BanII G_rGCy'C
Cuts at: 166 251 326 1606 2636 166
Size: 85 75 1280 1030 2124
Fragments arranged by size:
2124 1280 1030 85 75
BbeI G_GCGC'C
Cuts at: 2026 2274 2026
Size: 248 4346
BbsI GAAGACnn'nnnn_
Cuts at: 944 1120 944
Size: 176 4418
BbvCI CC'TCA_GC
Cuts at: 513 513
Size: 4594
BciVI GTATCCnnnnn_n'
Cuts at: 2490 3253 4594 2490
Size: 763 1341 2490
Fragments arranged by size:
2490 1341 763
BclI T'GATC_A
Cuts at: 2112 2112
Size: 4594
BglII A'GATC_T
Cuts at: 2107 4146 2107
Size: 2039 2555
BlpI GC'TnA_GC
Cuts at: 1704 1704
Size: 4594
Bme1580I G_kGCm'C
Cuts at: 2217 2310 3368 2217
Size: 93 1058 3443
Fragments arranged by size:
3443 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 356 1804 1885 2220 4366 356
Size: 1448 81 335 2146 584
Fragments arranged by size:
2146 1448 584 335 81
BmtI G_CTAG'C
Cuts at: 521 521
Size: 4594
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1055 2959 1055
Size: 1904 2690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 539 883 266
Size: 273 344 3977
Fragments arranged by size:
3977 344 273
Bpu10I CC'TnA_GC
Cuts at: 513 648 1073 513
Size: 135 425 4034
Fragments arranged by size:
4034 425 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1256 1768 2647 3141 3439 1229
Size: 27 512 879 494 298 2384
Fragments arranged by size:
2384 879 512 494 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 4298 4298
Size: 4594
BsaAI yAC'GTr
Cuts at: 1584 1945 1584
Size: 361 4233
BsaBI GATnn'nnATC
Cuts at: 6 860 2106 4145 6
Size: 854 1246 2039 455
Fragments arranged by size:
2039 1246 854 455
BsaHI Gr'CG_yC
Cuts at: 2023 2271 2975 4471 2023
Size: 248 704 1496 2146
Fragments arranged by size:
2146 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1781 2302 2982 3256 3403 3814 1781
Size: 521 680 274 147 411 2561
Fragments arranged by size:
2561 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 264 282 294 312 537 567 1394 1424 264
Size: 18 12 18 225 30 827 30 3434
Fragments arranged by size:
3434 827 225 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 786 786
Size: 4594
BseYI C'CCAG_C
Cuts at: 2017 2775 3354 3850 2017
Size: 758 579 496 2761
Fragments arranged by size:
2761 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2134 2180 3390 104
Size: 2030 46 1210 1308
Fragments arranged by size:
2030 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1606 2384 2574 3368 1606
Size: 778 190 794 2832
Fragments arranged by size:
2832 794 778 190
BsmI GAATG_Cn'
Cuts at: 717 1092 1570 717
Size: 375 478 3741
Fragments arranged by size:
3741 478 375
Bsp1286I G_dGCh'C
Cuts at: 166 251 326 1606 2217 2310 2384 2574 2636
Size: 85 75 1280 611 93 74 190 62
Cuts at: 2636 3368 166
Size: 732 1392
Fragments arranged by size:
1392 1280 732 611 190 93 85 75
74 62
BspEI T'CCGG_A
Cuts at: 3814 3814
Size: 4594
BspHI T'CATG_A
Cuts at: 1344 3770 1344
Size: 2426 2168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 348 477 1642 1856 2158 2539 348
Size: 129 1165 214 302 381 2403
Fragments arranged by size:
2403 1165 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 2884 2938 4424 4589 50
Size: 2834 54 1486 165 55
Fragments arranged by size:
2834 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 768 968 2504 768
Size: 200 1536 2858
Fragments arranged by size:
2858 1536 200
BsrFI r'CCGG_y
Cuts at: 181 2590 2771 2982 181
Size: 2409 181 211 1793
Fragments arranged by size:
2409 1793 211 181
BssSI C'ACGA_G
Cuts at: 2863 3223 2863
Size: 360 4234
BstAPI GCAn_nnn'nTGC
Cuts at: 478 1150 1516 1969 478
Size: 672 366 453 3103
Fragments arranged by size:
3103 672 453 366
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4594
BstXI CCAn_nnnn'nTGG
Cuts at: 174 174
Size: 4594
BstYI r'GATC_y
Cuts at: 1 331 1617 2084 2107 2442 2688 3702 3788
Size: 330 1286 467 23 335 246 1014 86
Cuts at: 3788 3800 4146 1
Size: 12 346 449
Fragments arranged by size:
1286 1014 467 449 346 335 330 246
86 23 12
BtgI C'CryG_G
Cuts at: 173 1520 173
Size: 1347 3247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2542 2723 2886 2542
Size: 181 163 4250
Fragments arranged by size:
4250 181 163
BtsI GCAGTG_nn'
Cuts at: 1835 1835
Size: 4594
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 471 506 471
Size: 35 4559
DraI TTT'AAA
Cuts at: 1591 1591
Size: 4594
DrdI GACnn_nn'nnGTC
Cuts at: 534 2298 3158 534
Size: 1764 860 1970
Fragments arranged by size:
1970 1764 860
EaeI y'GGCC_r
Cuts at: 176 2177 2351 2742 2769 4116 176
Size: 2001 174 391 27 1347 654
Fragments arranged by size:
2001 1347 654 391 174 27
EagI C'GGCC_G
Cuts at: 2177 2177
Size: 4594
EarI CTCTTCn'nnn_
Cuts at: 2615 2825 2615
Size: 210 4384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2800 3112 3258 4404 2800
Size: 312 146 1146 2990
Fragments arranged by size:
2990 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1604 1604
Size: 4594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 432 539 871 883 1015 1146 1180 1318
Size: 166 107 332 12 132 131 34 138
Cuts at: 1318 2417 2849 3598 3866 266
Size: 1099 432 749 268 994
Fragments arranged by size:
1099 994 749 432 332 268 166 138
132 131 107 34 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1556 1556
Size: 4594
EcoO109I rG'GnC_Cy
Cuts at: 1731 1731
Size: 4594
EcoRI G'AATT_C
Cuts at: 1596 1596
Size: 4594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 982 2611 982
Size: 1629 2965
FspI TGC'GCA
Cuts at: 2373 2373
Size: 4594
HaeII r_GCGC'y
Cuts at: 2026 2274 3298 4277 2026
Size: 248 1024 979 2343
Fragments arranged by size:
2343 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 537 569 2434 2464 2466 2496 537
Size: 32 1865 30 2 30 2635
Fragments arranged by size:
2635 1865 32 30 30 2
HincII GTy'rAC
Cuts at: 725 1631 725
Size: 906 3688
KasI G'GCGC_C
Cuts at: 2022 2270 2022
Size: 248 4346
KpnI G_GTAC'C
Cuts at: 1612 1612
Size: 4594
MluI A'CGCG_T
Cuts at: 4027 4027
Size: 4594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3265 3449 3265
Size: 184 4410
MscI TGG'CCA
Cuts at: 2353 2353
Size: 4594
MslI CAynn'nnrTG
Cuts at: 172 1139 1349 2581 2708 172
Size: 967 210 1232 127 2058
Fragments arranged by size:
2058 1232 967 210 127
MspA1I CmG'CkG
Cuts at: 1703 2017 2377 3392 3637 1703
Size: 314 360 1015 245 2660
Fragments arranged by size:
2660 1015 360 314 245
NaeI GCC'GGC
Cuts at: 183 2773 183
Size: 2590 2004
NarI GG'CG_CC
Cuts at: 2023 2271 2023
Size: 248 4346
NcoI C'CATG_G
Cuts at: 1520 1520
Size: 4594
NgoMIV G'CCGG_C
Cuts at: 181 2771 181
Size: 2590 2004
NheI G'CTAG_C
Cuts at: 517 517
Size: 4594
NruI TCG'CGA
Cuts at: 2955 2955
Size: 4594
NspI r_CATG'y
Cuts at: 1645 2145 3054 1645
Size: 500 909 3185
Fragments arranged by size:
3185 909 500
PciI A'CATG_T
Cuts at: 3050 3050
Size: 4594
PflMI CCAn_nnn'nTGG
Cuts at: 1448 1448
Size: 4594
PfoI T'CCnGG_A
Cuts at: 151 151
Size: 4594
PmeI GTTT'AAAC
Cuts at: 1591 1591
Size: 4594
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3733 3765 3733
Size: 32 4562
PstI C_TGCA'G
Cuts at: 435 474 759 1639 2324 435
Size: 39 285 880 685 2705
Fragments arranged by size:
2705 880 685 285 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4594
PvuII CAG'CTG
Cuts at: 2017 2377 2017
Size: 360 4234
RsrII CG'GwC_CG
Cuts at: 2787 2787
Size: 4594
SacI G_AGCT'C
Cuts at: 1606 1606
Size: 4594
SalI G'TCGA_C
Cuts at: 1629 1629
Size: 4594
SapI GCTCTTCn'nnn_
Cuts at: 2615 2825 2615
Size: 210 4384
SbfI CC_TGCA'GG
Cuts at: 1639 1639
Size: 4594
ScaI AGT'ACT
Cuts at: 273 4111 273
Size: 3838 756
SfcI C'TryA_G
Cuts at: 33 395 431 470 737 755 1383 1556 1635
Size: 362 36 39 267 18 628 173 79
Cuts at: 1635 2320 3315 3506 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 995 685 628 362 267 191 173
79 39 36 18
SfoI GGC'GCC
Cuts at: 2024 2272 2024
Size: 248 4346
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4594
SgrAI Cr'CCGG_yG
Cuts at: 181 181
Size: 4594
SmaI CCC'GGG
Cuts at: 1614 1614
Size: 4594
SmlI C'TyrA_G
Cuts at: 967 1244 1271 1747 2662 3156 3418 967
Size: 277 27 476 915 494 262 2143
Fragments arranged by size:
2143 915 494 476 277 262 27
SnaBI TAC'GTA
Cuts at: 1584 1584
Size: 4594
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4594
SphI G_CATG'C
Cuts at: 1645 2145 1645
Size: 500 4094
StuI AGG'CCT
Cuts at: 629 770 629
Size: 141 4453
StyI C'CwwG_G
Cuts at: 771 1377 1520 1726 2080 771
Size: 606 143 206 354 3285
Fragments arranged by size:
3285 606 354 206 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1476 2175 2308 2767 2978 580
Size: 896 699 133 459 211 2196
Fragments arranged by size:
2196 896 699 459 211 133
TatI w'GTAC_w
Cuts at: 271 679 1202 4109 271
Size: 408 523 2907 756
Fragments arranged by size:
2907 756 523 408
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4111 4143 4111
Size: 32 4562
Tth111I GACn'n_nGTC
Cuts at: 2389 2389
Size: 4594
XbaI T'CTAG_A
Cuts at: 1623 1623
Size: 4594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2087 2087
Size: 4594
XmaI C'CCGG_G
Cuts at: 1612 1612
Size: 4594
XmnI GAAnn'nnTTC
Cuts at: 273 951 1393 1570 273
Size: 678 442 177 3297
Fragments arranged by size:
3297 678 442 177
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SrfI SspI SwaI
XhoI ZraI