Restriction map for
pF1KSDA0414
(Circular) (Six-base) MAPSORT of: pF1KSDA0414.seq Check: 4315 from: 1 to: 4538
pF1KSDA0414 4538 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1056 1056
Size: 4538
AatII G_ACGT'C
Cuts at: 230 402 230
Size: 172 4366
AccI GT'mk_AC
Cuts at: 473 1576 473
Size: 1103 3435
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 2363 2795 3542 3810 506
Size: 1857 432 747 268 1234
Fragments arranged by size:
1857 1234 747 432 268
AfeI AGC'GCT
Cuts at: 874 874
Size: 4538
AflIII A'CryG_T
Cuts at: 1890 2994 3971 1890
Size: 1104 977 2457
Fragments arranged by size:
2457 1104 977
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AhdI GACnn_n'nnGTC
Cuts at: 546 678 546
Size: 132 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 919
Size: 4538
AlwNI CAG_nnn'CTG
Cuts at: 192 1231 1843 3410 192
Size: 1039 612 1567 1320
Fragments arranged by size:
1567 1320 1039 612
ApaLI G'TGCA_C
Cuts at: 904 3308 904
Size: 2404 2134
ApoI r'AATT_y
Cuts at: 157 1348 1385 1542 3891 4147 157
Size: 1191 37 157 2349 256 548
Fragments arranged by size:
2349 1191 548 256 157 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 868 1558 868
Size: 690 3848
BamHI G'GATC_C
Cuts at: 854 1563 854
Size: 709 3829
BanI G'GyrC_C
Cuts at: 778 1554 1968 2216 2251 778
Size: 776 414 248 35 3065
Fragments arranged by size:
3065 776 414 248 35
BanII G_rGCy'C
Cuts at: 601 1552 2582 601
Size: 951 1030 2557
Fragments arranged by size:
2557 1030 951
BbeI G_GCGC'C
Cuts at: 782 1972 2220 782
Size: 1190 248 3100
Fragments arranged by size:
3100 1190 248
BbsI GAAGACnn'nnnn_
Cuts at: 1306 1306
Size: 4538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1290 1324 1290
Size: 34 4504
BciVI GTATCCnnnnn_n'
Cuts at: 1253 2436 3197 4538 1253
Size: 1183 761 1341 1253
Fragments arranged by size:
1341 1253 1183 761
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1502 1650 1502
Size: 148 4390
Bme1580I G_kGCm'C
Cuts at: 264 908 1136 2163 2256 3312 264
Size: 644 228 1027 93 1056 1490
Fragments arranged by size:
1490 1056 1027 644 228 93
BmrI ACTGGGnnnn_n'
Cuts at: 1750 1831 2166 4310 1750
Size: 81 335 2144 1978
Fragments arranged by size:
2144 1978 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 615 2905 615
Size: 2290 2248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 439 401
Size: 38 4500
Bpu10I CC'TnA_GC
Cuts at: 742 742
Size: 4538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1714 2593 3085 3383 1714
Size: 879 492 298 2869
Fragments arranged by size:
2869 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 861 1530 1891 861
Size: 669 361 3508
Fragments arranged by size:
3508 669 361
BsaBI GATnn'nnATC
Cuts at: 6 1121 2052 4089 6
Size: 1115 931 2037 455
Fragments arranged by size:
2037 1115 931 455
BsaHI Gr'CG_yC
Cuts at: 227 399 779 1969 2217 2919 4415 227
Size: 172 380 1190 248 702 1496 350
Fragments arranged by size:
1496 1190 702 380 350 248 172
BsaWI w'CCGG_w
Cuts at: 1102 1727 2248 2926 3200 3347 3758 1102
Size: 625 521 678 274 147 411 1882
Fragments arranged by size:
1882 678 625 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1434 1464 1434
Size: 30 4508
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 787 1003 673
Size: 114 216 4208
Fragments arranged by size:
4208 216 114
BseYI C'CCAG_C
Cuts at: 594 686 712 829 1963 2721 3298 3794 594
Size: 92 26 117 1134 758 577 496 1338
Fragments arranged by size:
1338 1134 758 577 496 117 92 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 991 973
Size: 18 4520
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 667 701 748 908 926 965 1552 2330 2520
Size: 34 47 160 18 39 587 778 190
Cuts at: 2520 3312 667
Size: 792 1893
Fragments arranged by size:
1893 792 778 587 190 160 47 39
34 18
BsmBI CGTCTCn'nnnn_
Cuts at: 234 234
Size: 4538
Bsp1286I G_dGCh'C
Cuts at: 264 601 667 701 748 908 926 965 1136
Size: 337 66 34 47 160 18 39 171
Cuts at: 1136 1552 2163 2256 2330 2520 2582 3312 264
Size: 416 611 93 74 190 62 730 1490
Fragments arranged by size:
1490 730 611 416 337 190 171 160
93 74 66 62 47 39 34 18
BspEI T'CCGG_A
Cuts at: 1102 3758 1102
Size: 2656 1882
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 716 1056 1150 1588 1802 2104 2485 716
Size: 340 94 438 214 302 381 2769
Fragments arranged by size:
2769 438 381 340 302 214 94
BsrBI CCG'CTC
Cuts at: 50 415 2830 2884 4368 4533 50
Size: 365 2415 54 1484 165 55
Fragments arranged by size:
2415 1484 365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2450 2450
Size: 4538
BsrFI r'CCGG_y
Cuts at: 1457 2536 2717 2926 1457
Size: 1079 181 209 3069
Fragments arranged by size:
3069 1079 209 181
BsrGI T'GTAC_A
Cuts at: 818 818
Size: 4538
BssSI C'ACGA_G
Cuts at: 1369 2809 3167 1369
Size: 1440 358 2740
Fragments arranged by size:
2740 1440 358
BstAPI GCAn_nnn'nTGC
Cuts at: 969 1915 969
Size: 946 3592
BstBI TT'CG_AA
Cuts at: 110 2899 110
Size: 2789 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 837 837
Size: 4538
BstYI r'GATC_y
Cuts at: 1 854 1563 2030 2053 2388 2634 3646 3732
Size: 853 709 467 23 335 246 1012 86
Cuts at: 3732 3744 4090 1
Size: 12 346 449
Fragments arranged by size:
1012 853 709 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1088 125
Size: 963 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 543 1781 543
Size: 1238 3300
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DraIII CAC_nnn'GTG
Cuts at: 969 969
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 2244 3102 2244
Size: 858 3680
EaeI y'GGCC_r
Cuts at: 2123 2297 2688 2715 4060 2123
Size: 174 391 27 1345 2601
Fragments arranged by size:
2601 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 650 1088 1089 2561 2771 650
Size: 438 1 1472 210 2417
Fragments arranged by size:
2417 1472 438 210 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3056 3202 4348 2746
Size: 310 146 1146 2936
Fragments arranged by size:
2936 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1550 1550
Size: 4538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 439 506 2363 2795 3542 3810 401
Size: 38 67 1857 432 747 268 1129
Fragments arranged by size:
1857 1129 747 432 268 67 38
EcoO109I rG'GnC_Cy
Cuts at: 1677 1677
Size: 4538
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 292 2557 292
Size: 2265 2273
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 207 782 794 876 1972 2220 3242 4221 207
Size: 575 12 82 1096 248 1022 979 524
Fragments arranged by size:
1096 1022 979 575 524 248 82 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 664 667 696 699 2380 2410 2412
Size: 32 100 3 29 3 1681 30 2
Cuts at: 2412 2442 532
Size: 30 2628
Fragments arranged by size:
2628 1681 100 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 1577 1577
Size: 4538
HindIII A'AGCT_T
Cuts at: 447 1205 447
Size: 758 3780
KasI G'GCGC_C
Cuts at: 778 1968 2216 778
Size: 1190 248 3100
Fragments arranged by size:
3100 1190 248
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3393 3209
Size: 184 4354
MscI TGG'CCA
Cuts at: 2299 2299
Size: 4538
MslI CAynn'nnrTG
Cuts at: 536 751 901 1300 1460 2527 2654 536
Size: 215 150 399 160 1067 127 2420
Fragments arranged by size:
2420 1067 399 215 160 150 127
MspA1I CmG'CkG
Cuts at: 442 1649 1963 2323 3336 3581 442
Size: 1207 314 360 1013 245 1399
Fragments arranged by size:
1399 1207 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 779 1969 2217 779
Size: 1190 248 3100
Fragments arranged by size:
3100 1190 248
NcoI C'CATG_G
Cuts at: 125 1088 125
Size: 963 3575
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 469 1305 1465 1591 2091 2998 469
Size: 836 160 126 500 907 2009
Fragments arranged by size:
2009 907 836 500 160 126
PciI A'CATG_T
Cuts at: 2994 2994
Size: 4538
PflMI CCAn_nnn'nTGG
Cuts at: 1371 1371
Size: 4538
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PmlI CAC'GTG
Cuts at: 861 861
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PsiI TTA'TAA
Cuts at: 569 569
Size: 4538
PstI C_TGCA'G
Cuts at: 1585 2270 1585
Size: 685 3853
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 1963 2323 1963
Size: 360 4178
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 1552 1552
Size: 4538
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 1089 2561 2771 1089
Size: 1472 210 2856
Fragments arranged by size:
2856 1472 210
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SexAI A'CCwGG_T
Cuts at: 305 926 305
Size: 621 3917
SfcI C'TryA_G
Cuts at: 33 184 506 1581 2266 3259 3450 33
Size: 151 322 1075 685 993 191 1121
Fragments arranged by size:
1121 1075 993 685 322 191 151
SfoI GGC'GCC
Cuts at: 780 1970 2218 780
Size: 1190 248 3100
Fragments arranged by size:
3100 1190 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 1693 2608 3100 3362 1693
Size: 915 492 262 2869
Fragments arranged by size:
2869 915 492 262
SnaBI TAC'GTA
Cuts at: 1530 1530
Size: 4538
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1305 1591 2091 1305
Size: 286 500 3752
Fragments arranged by size:
3752 500 286
SspI AAT'ATT
Cuts at: 1176 1176
Size: 4538
StyI C'CwwG_G
Cuts at: 125 243 1088 1672 2026 125
Size: 118 845 584 354 2637
Fragments arranged by size:
2637 845 584 354 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1300 2121 2254 2713 2922 1300
Size: 821 133 459 209 2916
Fragments arranged by size:
2916 821 459 209 133
TatI w'GTAC_w
Cuts at: 818 1474 4053 818
Size: 656 2579 1303
Fragments arranged by size:
2579 1303 656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4506
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2033 2033
Size: 4538
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
XmnI GAAnn'nnTTC
Cuts at: 1264 1264
Size: 4538
ZraI GAC'GTC
Cuts at: 228 400 228
Size: 172 4366
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AleI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BglI BmgBI BmtI BsiWI BsmI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI StuI SwaI XhoI