Restriction map for
pF1KSDA0422
(Circular) (Six-base) MAPSORT of: pF1KSDA0422.seq Check: 138 from: 1 to: 6482
pF1KSDA0422 6482 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 220 486 1124 2700 3238 220
Size: 266 638 1576 538 3464
Fragments arranged by size:
3464 1576 638 538 266
AccI GT'mk_AC
Cuts at: 863 1481 2719 3520 863
Size: 618 1238 801 3825
Fragments arranged by size:
3825 1238 801 618
Acc65I G'GTAC_C
Cuts at: 3498 3498
Size: 6482
AclI AA'CG_TT
Cuts at: 6322 6322
Size: 6482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4307 4739 5486 5754 4307
Size: 432 747 268 5035
Fragments arranged by size:
5035 747 432 268
AflIII A'CryG_T
Cuts at: 1742 2405 3834 4938 5915 1742
Size: 663 1429 1104 977 2309
Fragments arranged by size:
2309 1429 1104 977 663
AgeI A'CCGG_T
Cuts at: 4870 4870
Size: 6482
AleI CACnn'nnGTG
Cuts at: 616 1463 616
Size: 847 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1010 1323 2257 1010
Size: 313 934 5235
Fragments arranged by size:
5235 934 313
AlwNI CAG_nnn'CTG
Cuts at: 1323 1695 3787 5354 1323
Size: 372 2092 1567 2451
Fragments arranged by size:
2451 2092 1567 372
ApaI G_GGCC'C
Cuts at: 304 3238 3463 304
Size: 2934 225 3323
Fragments arranged by size:
3323 2934 225
ApaLI G'TGCA_C
Cuts at: 1308 1564 5252 1308
Size: 256 3688 2538
Fragments arranged by size:
3688 2538 256
ApoI r'AATT_y
Cuts at: 3486 5835 6091 3486
Size: 2349 256 3877
Fragments arranged by size:
3877 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6482
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6482
AvaI C'yCGr_G
Cuts at: 224 1442 1676 1841 2852 3502 224
Size: 1218 234 165 1011 650 3204
Fragments arranged by size:
3204 1218 1011 650 234 165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 395 428 1675 1708 395
Size: 33 1247 33 5169
Fragments arranged by size:
5169 1247 33 33
BamHI G'GATC_C
Cuts at: 2121 3507 2121
Size: 1386 5096
BanI G'GyrC_C
Cuts at: 212 311 444 456 482 845 994 1786 1963
Size: 99 133 12 26 363 149 792 177
Cuts at: 1963 3498 3912 4160 4195 212
Size: 1535 414 248 35 2499
Fragments arranged by size:
2499 1535 792 414 363 248 177 149
133 99 35 26 12
BanII G_rGCy'C
Cuts at: 304 707 1346 2507 3238 3264 3463 3496 4526
Size: 403 639 1161 731 26 199 33 1030
Cuts at: 4526 304
Size: 2260
Fragments arranged by size:
2260 1161 1030 731 639 403 199 33
26
BbeI G_GCGC'C
Cuts at: 216 448 486 1790 3916 4164 216
Size: 232 38 1304 2126 248 2534
Fragments arranged by size:
2534 2126 1304 248 232 38
BbsI GAAGACnn'nnnn_
Cuts at: 1199 1220 2154 2445 1199
Size: 21 934 291 5236
Fragments arranged by size:
5236 934 291 21
BbvCI CC'TCA_GC
Cuts at: 437 653 910 437
Size: 216 257 6009
Fragments arranged by size:
6009 257 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1111 1145 1822 1856 1111
Size: 34 677 34 5737
Fragments arranged by size:
5737 677 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 396 4380 5141 6482 396
Size: 3984 761 1341 396
Fragments arranged by size:
3984 1341 761 396
BclI T'GATC_A
Cuts at: 2037 4002 2037
Size: 1965 4517
BglI GCCn_nnn'nGGC
Cuts at: 667 1658 1730 1822 1931 667
Size: 991 72 92 109 5218
Fragments arranged by size:
5218 991 109 92 72
BglII A'GATC_T
Cuts at: 1232 1397 3997 6034 1232
Size: 165 2600 2037 1680
Fragments arranged by size:
2600 2037 1680 165
BlpI GC'TnA_GC
Cuts at: 2705 3594 2705
Size: 889 5593
Bme1580I G_kGCm'C
Cuts at: 304 316 461 850 1312 1568 1966 2154 2346
Size: 12 145 389 462 256 398 188 192
Cuts at: 2346 3238 3463 4107 4200 5256 304
Size: 892 225 644 93 1056 1530
Fragments arranged by size:
1530 1056 892 644 462 398 389 256
225 192 188 145 93 12
BmgBI CAC'GTC
Cuts at: 2832 2832
Size: 6482
BmrI ACTGGGnnnn_n'
Cuts at: 1299 3232 3694 3775 4110 6254 1299
Size: 1933 462 81 335 2144 1527
Fragments arranged by size:
2144 1933 1527 462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1515 4849 1515
Size: 3334 3148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 2971 3415 553
Size: 2418 444 3620
Fragments arranged by size:
3620 2418 444
Bpu10I CC'TnA_GC
Cuts at: 437 653 910 2008 437
Size: 216 257 1098 4911
Fragments arranged by size:
4911 1098 257 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 2116 3658 4537 5029 5327 1735
Size: 381 1542 879 492 298 2890
Fragments arranged by size:
2890 1542 879 492 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 1078 2596 6186 1078
Size: 1518 3590 1374
Fragments arranged by size:
3590 1518 1374
BsaAI yAC'GTr
Cuts at: 765 1510 3474 3835 765
Size: 745 1964 361 3412
Fragments arranged by size:
3412 1964 745 361
BsaBI GATnn'nnATC
Cuts at: 6 1396 3996 6033 6
Size: 1390 2600 2037 455
Fragments arranged by size:
2600 2037 1390 455
BsaHI Gr'CG_yC
Cuts at: 213 329 445 483 1574 1787 2576 3913 4161
Size: 116 116 38 1091 213 789 1337 248
Cuts at: 4161 4863 6359 213
Size: 702 1496 336
Fragments arranged by size:
1496 1337 1091 789 702 336 248 213
116 116 38
BsaWI w'CCGG_w
Cuts at: 338 2798 3671 4192 4870 5144 5291 5702 338
Size: 2460 873 521 678 274 147 411 1118
Fragments arranged by size:
2460 1118 873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 556 2126 2156 526
Size: 30 1570 30 4852
Fragments arranged by size:
4852 1570 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 1822 2785 2794 3043 871
Size: 951 963 9 249 4310
Fragments arranged by size:
4310 963 951 249 9
BseYI C'CCAG_C
Cuts at: 283 369 771 1166 1874 2529 2538 3463 3907
Size: 86 402 395 708 655 9 925 444
Cuts at: 3907 4665 5242 5738 283
Size: 758 577 496 1027
Fragments arranged by size:
1027 925 758 708 655 577 496 444
402 395 86 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 514 811 1002 1023 2443 225
Size: 289 297 191 21 1420 4264
Fragments arranged by size:
4264 1420 297 289 191 21
BsiEI CG_ry'CG
Cuts at: 104 3339 4024 4070 5278 104
Size: 3235 685 46 1208 1308
Fragments arranged by size:
3235 1308 1208 685 46
BsiHKAI G_wGCw'C
Cuts at: 1312 1346 1568 3194 3496 4274 4464 5256 1312
Size: 34 222 1626 302 778 190 792 2538
Fragments arranged by size:
2538 1626 792 778 302 222 190 34
Bsp1286I G_dGCh'C
Cuts at: 304 316 461 707 850 1312 1346 1568 1966
Size: 12 145 246 143 462 34 222 398
Cuts at: 1966 2154 2346 2507 3194 3238 3264 3463 3496
Size: 188 192 161 687 44 26 199 33
Cuts at: 3496 4107 4200 4274 4464 4526 5256 304
Size: 611 93 74 190 62 730 1530
Fragments arranged by size:
1530 730 687 611 462 398 246 222
199 192 190 188 161 145 143 93
74 62 44 34 33 26 12
BspEI T'CCGG_A
Cuts at: 338 5702 338
Size: 5364 1118
BspHI T'CATG_A
Cuts at: 1328 5658 1328
Size: 4330 2152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 220 486 783 1124 2700 3238 3532 3746 4048
Size: 266 297 341 1576 538 294 214 302
Cuts at: 4048 4429 220
Size: 381 2273
Fragments arranged by size:
2273 1576 538 381 341 302 297 294
266 214
BsrBI CCG'CTC
Cuts at: 50 1143 2336 3033 3125 4774 4828 6312 6477
Size: 1093 1193 697 92 1649 54 1484 165
Cuts at: 6477 50
Size: 55
Fragments arranged by size:
1649 1484 1193 1093 697 165 92 55
54
BsrDI GCAATG_nn'
Cuts at: 198 2396 2871 2918 4394 198
Size: 2198 475 47 1476 2286
Fragments arranged by size:
2286 2198 1476 475 47
BsrFI r'CCGG_y
Cuts at: 722 2063 4480 4661 4870 722
Size: 1341 2417 181 209 2334
Fragments arranged by size:
2417 2334 1341 209 181
BssSI C'ACGA_G
Cuts at: 4753 5111 4753
Size: 358 6124
BstAPI GCAn_nnn'nTGC
Cuts at: 3859 3859
Size: 6482
BstBI TT'CG_AA
Cuts at: 110 4843 110
Size: 4733 1749
BstEII G'GTnAC_C
Cuts at: 3348 3348
Size: 6482
BstXI CCAn_nnnn'nTGG
Cuts at: 753 1503 1638 1854 2832 753
Size: 750 135 216 978 4403
Fragments arranged by size:
4403 978 750 216 135
BstYI r'GATC_y
Cuts at: 1 1232 1397 2091 2121 2222 3507 3974 3997
Size: 1231 165 694 30 101 1285 467 23
Cuts at: 3997 4332 4578 5590 5676 5688 6034 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1285 1231 1012 694 467 449 346 335
246 165 101 86 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1387 1985 2300 3087 1387
Size: 598 315 787 4782
Fragments arranged by size:
4782 787 598 315
BtgI C'CryG_G
Cuts at: 216 963 1089 1178 1725 1853 2580 3353 216
Size: 747 126 89 547 128 727 773 3345
Fragments arranged by size:
3345 773 747 727 547 128 126 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 852 2985 4432 4613 4776 852
Size: 2133 1447 181 163 2558
Fragments arranged by size:
2558 2133 1447 181 163
BtsI GCAGTG_nn'
Cuts at: 389 794 1310 1380 1458 1566 1684 3134 3725
Size: 405 516 70 78 108 118 1450 591
Cuts at: 3725 389
Size: 3146
Fragments arranged by size:
3146 1450 591 516 405 118 108 78
70
ClaI AT'CG_AT
Cuts at: 2021 2021
Size: 6482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2532 2567 2532
Size: 35 6447
DraI TTT'AAA
Cuts at: 3481 3481
Size: 6482
DrdI GACnn_nn'nnGTC
Cuts at: 4188 5046 4188
Size: 858 5624
EaeI y'GGCC_r
Cuts at: 524 1628 1659 1823 2441 2483 4067 4241 4632
Size: 1104 31 164 618 42 1584 174 391
Cuts at: 4632 4659 6004 524
Size: 27 1345 1002
Fragments arranged by size:
1584 1345 1104 1002 618 391 174 164
42 31 27
EagI C'GGCC_G
Cuts at: 4067 4067
Size: 6482
EarI CTCTTCn'nnn_
Cuts at: 4505 4715 4505
Size: 210 6272
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 724 1912 4690 5000 5146 6292 724
Size: 1188 2778 310 146 1146 914
Fragments arranged by size:
2778 1188 1146 914 310 146
EcoICRI GAG'CTC
Cuts at: 1344 3494 1344
Size: 2150 4332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 2971 3415 4307 4739 5486 5754 553
Size: 2418 444 892 432 747 268 1281
Fragments arranged by size:
2418 1281 892 747 444 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3364 3364
Size: 6482
EcoO109I rG'GnC_Cy
Cuts at: 301 2304 2533 3330 3459 3460 3621 301
Size: 2003 229 797 129 1 161 3162
Fragments arranged by size:
3162 2003 797 229 161 129 1
EcoRI G'AATT_C
Cuts at: 3486 3486
Size: 6482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4501 4501
Size: 6482
FspI TGC'GCA
Cuts at: 1316 4263 1316
Size: 2947 3535
HaeII r_GCGC'y
Cuts at: 216 448 486 540 806 1071 1790 2692 2862
Size: 232 38 54 266 265 719 902 170
Cuts at: 2862 3916 4164 5186 6165 216
Size: 1054 248 1022 979 533
Fragments arranged by size:
1054 1022 979 902 719 533 266 265
248 232 170 54 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1626 2076 2108 3395 3427 4324 4354 4356
Size: 32 450 32 1287 32 897 30 2
Cuts at: 4356 4386 1594
Size: 30 3690
Fragments arranged by size:
3690 1287 897 450 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2720 3521 2720
Size: 801 5681
KasI G'GCGC_C
Cuts at: 212 444 482 1786 3912 4160 212
Size: 232 38 1304 2126 248 2534
Fragments arranged by size:
2534 2126 1304 248 232 38
KpnI G_GTAC'C
Cuts at: 3502 3502
Size: 6482
MluI A'CGCG_T
Cuts at: 1742 5915 1742
Size: 4173 2309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5153 5337 5153
Size: 184 6298
MscI TGG'CCA
Cuts at: 1630 1825 2443 2485 4243 1630
Size: 195 618 42 1758 3869
Fragments arranged by size:
3869 1758 618 195 42
MslI CAynn'nnrTG
Cuts at: 616 712 751 892 1183 1253 1305 1463 1540
Size: 96 39 141 291 70 52 158 77
Cuts at: 1540 2212 3076 3185 4471 4598 616
Size: 672 864 109 1286 127 2500
Fragments arranged by size:
2500 1286 864 672 291 158 141 127
109 96 77 70 52 39
MspA1I CmG'CkG
Cuts at: 424 549 616 914 1091 1556 1836 2043 2629
Size: 125 67 298 177 465 280 207 586
Cuts at: 2629 3045 3393 3593 3907 4267 5280 5525 424
Size: 416 348 200 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 586 465 416 360 348 314
298 280 245 207 200 177 125 67
NaeI GCC'GGC
Cuts at: 4663 4663
Size: 6482
NarI GG'CG_CC
Cuts at: 213 445 483 1787 3913 4161 213
Size: 232 38 1304 2126 248 2534
Fragments arranged by size:
2534 2126 1304 248 232 38
NcoI C'CATG_G
Cuts at: 1178 1853 2580 1178
Size: 675 727 5080
Fragments arranged by size:
5080 727 675
NgoMIV G'CCGG_C
Cuts at: 4661 4661
Size: 6482
NspI r_CATG'y
Cuts at: 897 1539 2409 2427 2704 3535 4035 4942 897
Size: 642 870 18 277 831 500 907 2437
Fragments arranged by size:
2437 907 870 831 642 500 277 18
PasI CC'CwG_GG
Cuts at: 390 390
Size: 6482
PciI A'CATG_T
Cuts at: 2405 4938 2405
Size: 2533 3949
PflMI CCAn_nnn'nTGG
Cuts at: 131 1726 2586 2847 131
Size: 1595 860 261 3766
Fragments arranged by size:
3766 1595 860 261
PfoI T'CCnGG_A
Cuts at: 266 1897 2110 266
Size: 1631 213 4638
Fragments arranged by size:
4638 1631 213
PmeI GTTT'AAAC
Cuts at: 3481 3481
Size: 6482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3051 5621 5653 3019
Size: 32 2570 32 3848
Fragments arranged by size:
3848 2570 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 3330 2533
Size: 797 5685
PspOMI G'GGCC_C
Cuts at: 300 3234 3459 300
Size: 2934 225 3323
Fragments arranged by size:
3323 2934 225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1690 1722 1690
Size: 32 6450
PstI C_TGCA'G
Cuts at: 1121 1691 1835 2634 3529 4214 1121
Size: 570 144 799 895 685 3389
Fragments arranged by size:
3389 895 799 685 570 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6482
PvuII CAG'CTG
Cuts at: 2043 2629 3045 3393 3907 4267 2043
Size: 586 416 348 514 360 4258
Fragments arranged by size:
4258 586 514 416 360 348
RsrII CG'GwC_CG
Cuts at: 4677 4677
Size: 6482
SacI G_AGCT'C
Cuts at: 1346 3496 1346
Size: 2150 4332
SacII CC_GC'GG
Cuts at: 1092 1092
Size: 6482
SalI G'TCGA_C
Cuts at: 2718 3519 2718
Size: 801 5681
SanDI GG'GwC_CC
Cuts at: 2533 3330 2533
Size: 797 5685
SapI GCTCTTCn'nnn_
Cuts at: 4505 4715 4505
Size: 210 6272
SbfI CC_TGCA'GG
Cuts at: 3529 3529
Size: 6482
ScaI AGT'ACT
Cuts at: 2107 5999 2107
Size: 3892 2590
SexAI A'CCwGG_T
Cuts at: 3365 3365
Size: 6482
SfcI C'TryA_G
Cuts at: 33 1117 1687 1831 2630 2749 2785 3525 4210
Size: 1084 570 144 799 119 36 740 685
Cuts at: 4210 5203 5394 33
Size: 993 191 1121
Fragments arranged by size:
1121 1084 993 799 740 685 570 191
144 119 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 1658 1822 1658
Size: 164 6318
SfoI GGC'GCC
Cuts at: 214 446 484 1788 3914 4162 214
Size: 232 38 1304 2126 248 2534
Fragments arranged by size:
2534 2126 1304 248 232 38
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6482
SmaI CCC'GGG
Cuts at: 1444 2854 3504 1444
Size: 1410 650 4422
Fragments arranged by size:
4422 1410 650
SmlI C'TyrA_G
Cuts at: 1750 2095 3637 4552 5044 5306 1750
Size: 345 1542 915 492 262 2926
Fragments arranged by size:
2926 1542 915 492 345 262
SnaBI TAC'GTA
Cuts at: 3474 3474
Size: 6482
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6482
SphI G_CATG'C
Cuts at: 897 2704 3535 4035 897
Size: 1807 831 500 3344
Fragments arranged by size:
3344 1807 831 500
SrfI GCCC'GGGC
Cuts at: 1444 1444
Size: 6482
StuI AGG'CCT
Cuts at: 1075 1918 1075
Size: 843 5639
StyI C'CwwG_G
Cuts at: 1178 1578 1853 1904 1913 2580 2825 3380 3616
Size: 400 275 51 9 667 245 555 236
Cuts at: 3616 3970 1178
Size: 354 3690
Fragments arranged by size:
3690 667 555 400 354 275 245 236
51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 741 1981 3353 4065 4198 4657 4866 297
Size: 444 1240 1372 712 133 459 209 1913
Fragments arranged by size:
1913 1372 1240 712 459 444 209 133
TatI w'GTAC_w
Cuts at: 2105 2276 2408 5997 2105
Size: 171 132 3589 2590
Fragments arranged by size:
3589 2590 171 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 733 765 1032 1064 5999 6031 733
Size: 32 267 32 4935 32 1184
Fragments arranged by size:
4935 1184 267 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1255 4279 1255
Size: 3024 3458
XbaI T'CTAG_A
Cuts at: 3513 3513
Size: 6482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3162 3217 3977 3162
Size: 55 760 5667
Fragments arranged by size:
5667 760 55
XmaI C'CCGG_G
Cuts at: 1442 2852 3502 1442
Size: 1410 650 4422
Fragments arranged by size:
4422 1410 650
XmnI GAAnn'nnTTC
Cuts at: 514 514
Size: 6482
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
SgrAI SspI SwaI XhoI ZraI