Restriction map for
pF1KSDA0426
(Circular) (Six-base) MAPSORT of: pF1KSDA0426.seq Check: 5350 from: 1 to: 4949
pF1KSDA0426 4949 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 579 1987 579
Size: 1408 3541
Acc65I G'GTAC_C
Cuts at: 1965 1965
Size: 4949
AclI AA'CG_TT
Cuts at: 4789 4789
Size: 4949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 746 833 1461 1794 2774 3206 3953 4221
Size: 491 87 628 333 980 432 747 268
Cuts at: 4221 255
Size: 983
Fragments arranged by size:
983 980 747 628 491 432 333 268
87
AflIII A'CryG_T
Cuts at: 2301 3405 4382 2301
Size: 1104 977 2868
Fragments arranged by size:
2868 1104 977
AgeI A'CCGG_T
Cuts at: 3337 3337
Size: 4949
AhdI GACnn_n'nnGTC
Cuts at: 299 575 299
Size: 276 4673
AlwNI CAG_nnn'CTG
Cuts at: 348 381 408 2254 3821 348
Size: 33 27 1846 1567 1476
Fragments arranged by size:
1846 1567 1476 33 27
ApaLI G'TGCA_C
Cuts at: 1287 3719 1287
Size: 2432 2517
ApoI r'AATT_y
Cuts at: 705 760 1508 1592 1953 4302 4558 705
Size: 55 748 84 361 2349 256 1096
Fragments arranged by size:
2349 1096 748 361 256 84 55
AseI AT'TA_AT
Cuts at: 20 20
Size: 4949
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 1969 1969
Size: 4949
BamHI G'GATC_C
Cuts at: 1007 1974 1007
Size: 967 3982
BanI G'GyrC_C
Cuts at: 1354 1965 2379 2627 2662 1354
Size: 611 414 248 35 3641
Fragments arranged by size:
3641 611 414 248 35
BanII G_rGCy'C
Cuts at: 431 833 1897 1963 2993 431
Size: 402 1064 66 1030 2387
Fragments arranged by size:
2387 1064 1030 402 66
BbeI G_GCGC'C
Cuts at: 1358 2383 2631 1358
Size: 1025 248 3676
Fragments arranged by size:
3676 1025 248
BbsI GAAGACnn'nnnn_
Cuts at: 249 754 1831 249
Size: 505 1077 3367
Fragments arranged by size:
3367 1077 505
BbvCI CC'TCA_GC
Cuts at: 349 349
Size: 4949
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 317 351 317
Size: 34 4915
BciVI GTATCCnnnnn_n'
Cuts at: 2847 3608 4949 2847
Size: 761 1341 2847
Fragments arranged by size:
2847 1341 761
BclI T'GATC_A
Cuts at: 2469 2469
Size: 4949
BglII A'GATC_T
Cuts at: 1932 2464 4501 1932
Size: 532 2037 2380
Fragments arranged by size:
2380 2037 532
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 1291 2574 2667 3723 1291
Size: 1283 93 1056 2517
Fragments arranged by size:
2517 1283 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 551 593 1526 1778 2161 2242 2577 4721 551
Size: 42 933 252 383 81 335 2144 779
Fragments arranged by size:
2144 933 779 383 335 252 81 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 546 3316 546
Size: 2770 2179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 415 418 508 595 629 828 1037 1121
Size: 12 3 90 87 34 199 209 84
Cuts at: 1121 1622 1706 1895 403
Size: 501 84 189 3457
Fragments arranged by size:
3457 501 209 199 189 90 87 84
84 34 12 3
Bpu10I CC'TnA_GC
Cuts at: 349 1891 349
Size: 1542 3407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2125 3004 3496 3794 2125
Size: 879 492 298 3280
Fragments arranged by size:
3280 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 352 534 4653 352
Size: 182 4119 648
Fragments arranged by size:
4119 648 182
BsaAI yAC'GTr
Cuts at: 1941 2302 1941
Size: 361 4588
BsaBI GATnn'nnATC
Cuts at: 6 2463 4500 6
Size: 2457 2037 455
Fragments arranged by size:
2457 2037 455
BsaHI Gr'CG_yC
Cuts at: 1355 2380 2628 3330 4826 1355
Size: 1025 248 702 1496 1478
Fragments arranged by size:
1496 1478 1025 702 248
BsaWI w'CCGG_w
Cuts at: 2138 2659 3337 3611 3758 4169 2138
Size: 521 678 274 147 411 2918
Fragments arranged by size:
2918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 512 542 512
Size: 30 4919
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 484 439
Size: 45 4904
BseYI C'CCAG_C
Cuts at: 626 2374 3132 3709 4205 626
Size: 1748 758 577 496 1370
Fragments arranged by size:
1748 1370 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 463
Size: 4949
BsiEI CG_ry'CG
Cuts at: 104 2491 2537 3745 104
Size: 2387 46 1208 1308
Fragments arranged by size:
2387 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 1291 1897 1963 2741 2931 3723 431
Size: 860 606 66 778 190 792 1657
Fragments arranged by size:
1657 860 792 778 606 190 66
BsmI GAATG_Cn'
Cuts at: 1725 1725
Size: 4949
BsmBI CGTCTCn'nnnn_
Cuts at: 562 562
Size: 4949
Bsp1286I G_dGCh'C
Cuts at: 431 833 1291 1897 1963 2574 2667 2741 2931
Size: 402 458 606 66 611 93 74 190
Cuts at: 2931 2993 3723 431
Size: 62 730 1657
Fragments arranged by size:
1657 730 611 606 458 402 190 93
74 66 62
BspEI T'CCGG_A
Cuts at: 4169 4169
Size: 4949
BspHI T'CATG_A
Cuts at: 4125 4125
Size: 4949
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 516 1999 2213 2515 2896 233
Size: 283 1483 214 302 381 2286
Fragments arranged by size:
2286 1483 381 302 283 214
BsrBI CCG'CTC
Cuts at: 50 3241 3295 4779 4944 50
Size: 3191 54 1484 165 55
Fragments arranged by size:
3191 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2861 2861
Size: 4949
BsrFI r'CCGG_y
Cuts at: 1145 1351 2947 3128 3337 1145
Size: 206 1596 181 209 2757
Fragments arranged by size:
2757 1596 209 206 181
BssSI C'ACGA_G
Cuts at: 803 3220 3578 803
Size: 2417 358 2174
Fragments arranged by size:
2417 2174 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2326 2326
Size: 4949
BstBI TT'CG_AA
Cuts at: 110 3310 110
Size: 3200 1749
BstYI r'GATC_y
Cuts at: 1 1007 1259 1932 1974 2441 2464 2799 3045
Size: 1006 252 673 42 467 23 335 246
Cuts at: 3045 4057 4143 4155 4501 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 1006 673 467 449 346 335 252
246 86 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 422 422
Size: 4949
BtgI C'CryG_G
Cuts at: 125 1059 125
Size: 934 4015
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2899 3080 3243 2899
Size: 181 163 4605
Fragments arranged by size:
4605 181 163
BtsI GCAGTG_nn'
Cuts at: 2192 2192
Size: 4949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 1362
Size: 35 4914
DraI TTT'AAA
Cuts at: 710 1948 710
Size: 1238 3711
DraIII CAC_nnn'GTG
Cuts at: 1295 1295
Size: 4949
DrdI GACnn_nn'nnGTC
Cuts at: 2655 3513 2655
Size: 858 4091
EaeI y'GGCC_r
Cuts at: 1143 2534 2708 3099 3126 4471 1143
Size: 1391 174 391 27 1345 1621
Fragments arranged by size:
1621 1391 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2534 2534
Size: 4949
EarI CTCTTCn'nnn_
Cuts at: 188 821 2972 3182 188
Size: 633 2151 210 1955
Fragments arranged by size:
2151 1955 633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3157 3467 3613 4759 3157
Size: 310 146 1146 3347
Fragments arranged by size:
3347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 429 1895 1961 429
Size: 1466 66 3417
Fragments arranged by size:
3417 1466 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 403 415 418 508 595 629 746 828
Size: 148 12 3 90 87 34 117 82
Cuts at: 828 833 1037 1121 1461 1622 1706 1794 1895
Size: 5 204 84 340 161 84 88 101
Cuts at: 1895 2774 3206 3953 4221 255
Size: 879 432 747 268 983
Fragments arranged by size:
983 879 747 432 340 268 204 161
148 117 101 90 88 87 84 84
82 34 12 5 3
EcoNI CCTnn'n_nnAGG
Cuts at: 421 421
Size: 4949
EcoO109I rG'GnC_Cy
Cuts at: 222 1694 2088 222
Size: 1472 394 3083
Fragments arranged by size:
3083 1472 394
EcoRI G'AATT_C
Cuts at: 1508 1592 1953 1508
Size: 84 361 4504
Fragments arranged by size:
4504 361 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4949
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 1358 2383 2631 3653 4632 1358
Size: 1025 248 1022 979 1675
Fragments arranged by size:
1675 1025 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 512 544 561 593 912 944 1643
Size: 32 195 32 17 32 319 32 699
Cuts at: 1643 1675 2791 2821 2823 2853 285
Size: 32 1116 30 2 30 2381
Fragments arranged by size:
2381 1116 699 319 195 32 32 32
32 32 30 30 17 2
HincII GTy'rAC
Cuts at: 1988 1988
Size: 4949
HindIII A'AGCT_T
Cuts at: 968 1052 968
Size: 84 4865
KasI G'GCGC_C
Cuts at: 1354 2379 2627 1354
Size: 1025 248 3676
Fragments arranged by size:
3676 1025 248
KpnI G_GTAC'C
Cuts at: 1969 1969
Size: 4949
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3620 3804 3620
Size: 184 4765
MscI TGG'CCA
Cuts at: 2710 2710
Size: 4949
MslI CAynn'nnrTG
Cuts at: 1205 2938 3065 1205
Size: 1733 127 3089
Fragments arranged by size:
3089 1733 127
MspA1I CmG'CkG
Cuts at: 2060 2374 2734 3747 3992 2060
Size: 314 360 1013 245 3017
Fragments arranged by size:
3017 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3130 3130
Size: 4949
NarI GG'CG_CC
Cuts at: 1355 2380 2628 1355
Size: 1025 248 3676
Fragments arranged by size:
3676 1025 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4949
NgoMIV G'CCGG_C
Cuts at: 3128 3128
Size: 4949
NspI r_CATG'y
Cuts at: 2002 2502 3409 2002
Size: 500 907 3542
Fragments arranged by size:
3542 907 500
PciI A'CATG_T
Cuts at: 3405 3405
Size: 4949
PflMI CCAn_nnn'nTGG
Cuts at: 463 463
Size: 4949
PfoI T'CCnGG_A
Cuts at: 930 930
Size: 4949
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 4088
Size: 32 4917
PpuMI rG'GwC_Cy
Cuts at: 222 222
Size: 4949
PsiI TTA'TAA
Cuts at: 1400 1400
Size: 4949
PstI C_TGCA'G
Cuts at: 1996 2681 1996
Size: 685 4264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 2374 2734 2374
Size: 360 4589
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 431 1897 1963 431
Size: 1466 66 3417
Fragments arranged by size:
3417 1466 66
SalI G'TCGA_C
Cuts at: 1986 1986
Size: 4949
SapI GCTCTTCn'nnn_
Cuts at: 821 2972 3182 821
Size: 2151 210 2588
Fragments arranged by size:
2588 2151 210
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 268 4466 268
Size: 4198 751
SfcI C'TryA_G
Cuts at: 33 1992 2677 3670 3861 33
Size: 1959 685 993 191 1121
Fragments arranged by size:
1959 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1356 2381 2629 1356
Size: 1025 248 3676
Fragments arranged by size:
3676 1025 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1971 1971
Size: 4949
SmlI C'TyrA_G
Cuts at: 2104 3019 3511 3773 2104
Size: 915 492 262 3280
Fragments arranged by size:
3280 915 492 262
SnaBI TAC'GTA
Cuts at: 1941 1941
Size: 4949
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 2002 2502 2002
Size: 500 4449
StuI AGG'CCT
Cuts at: 436 1241 436
Size: 805 4144
StyI C'CwwG_G
Cuts at: 125 2083 2437 125
Size: 1958 354 2637
Fragments arranged by size:
2637 1958 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 429 2532 2665 3124 3333 256
Size: 173 2103 133 459 209 1872
Fragments arranged by size:
2103 1872 459 209 173 133
TatI w'GTAC_w
Cuts at: 266 4464 266
Size: 4198 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 4917
Tth111I GACn'n_nGTC
Cuts at: 2746 2746
Size: 4949
XbaI T'CTAG_A
Cuts at: 1980 1980
Size: 4949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 295 2444 295
Size: 2149 2800
XmaI C'CCGG_G
Cuts at: 1969 1969
Size: 4949
XmnI GAAnn'nnTTC
Cuts at: 377 1474 1723 1844 377
Size: 1097 249 121 3482
Fragments arranged by size:
3482 1097 249 121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I ClaI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI