Restriction map for
pF1KSDA0438
(Circular) (Six-base) MAPSORT of: pF1KSDA0438.seq Check: 5500 from: 1 to: 5263
pF1KSDA0438 5263 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2172
Size: 5263
AccI GT'mk_AC
Cuts at: 2299 2299
Size: 5263
Acc65I G'GTAC_C
Cuts at: 2277 2277
Size: 5263
AclI AA'CG_TT
Cuts at: 5103 5103
Size: 5263
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1570 1580 1835 3086 3518 4267 4535 995
Size: 575 10 255 1251 432 749 268 1723
Fragments arranged by size:
1723 1251 749 575 432 268 255 10
AflIII A'CryG_T
Cuts at: 2613 3719 4696 2613
Size: 1106 977 3180
Fragments arranged by size:
3180 1106 977
AgeI A'CCGG_T
Cuts at: 3651 3651
Size: 5263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1825 1857 1825
Size: 32 5231
AlwNI CAG_nnn'CTG
Cuts at: 2566 4135 2566
Size: 1569 3694
ApaLI G'TGCA_C
Cuts at: 4033 4033
Size: 5263
ApoI r'AATT_y
Cuts at: 348 428 890 1622 1690 2265 4616 4872 348
Size: 80 462 732 68 575 2351 256 739
Fragments arranged by size:
2351 739 732 575 462 256 80 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 5263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5263
AvaI C'yCGr_G
Cuts at: 2281 2281
Size: 5263
BamHI G'GATC_C
Cuts at: 1254 1833 2286 1254
Size: 579 453 4231
Fragments arranged by size:
4231 579 453
BanI G'GyrC_C
Cuts at: 2277 2691 2939 2974 2277
Size: 414 248 35 4566
Fragments arranged by size:
4566 414 248 35
BanII G_rGCy'C
Cuts at: 2275 3305 2275
Size: 1030 4233
BbeI G_GCGC'C
Cuts at: 2695 2943 2695
Size: 248 5015
BbsI GAAGACnn'nnnn_
Cuts at: 246 893 1763 1963 246
Size: 647 870 200 3546
Fragments arranged by size:
3546 870 647 200
BciVI GTATCCnnnnn_n'
Cuts at: 507 3159 3922 5263 507
Size: 2652 763 1341 507
Fragments arranged by size:
2652 1341 763 507
BclI T'GATC_A
Cuts at: 2781 2781
Size: 5263
BglII A'GATC_T
Cuts at: 2776 4815 2776
Size: 2039 3224
BlpI GC'TnA_GC
Cuts at: 1872 2373 1872
Size: 501 4762
Bme1580I G_kGCm'C
Cuts at: 2886 2979 4037 2886
Size: 93 1058 4112
Fragments arranged by size:
4112 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 389 2473 2554 2889 5035 389
Size: 2084 81 335 2146 617
Fragments arranged by size:
2146 2084 617 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 920 1758 3628 920
Size: 838 1870 2555
Fragments arranged by size:
2555 1870 838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1728 1753 1728
Size: 25 5238
Bpu10I CC'TnA_GC
Cuts at: 291 1532 291
Size: 1241 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 1251 1467 2437 3316 3810 4108 890
Size: 361 216 970 879 494 298 2045
Fragments arranged by size:
2045 970 879 494 361 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 1972 4967 1972
Size: 2995 2268
BsaAI yAC'GTr
Cuts at: 2253 2614 2253
Size: 361 4902
BsaBI GATnn'nnATC
Cuts at: 6 2775 4814 6
Size: 2769 2039 455
Fragments arranged by size:
2769 2039 455
BsaHI Gr'CG_yC
Cuts at: 2692 2940 3644 5140 2692
Size: 248 704 1496 2815
Fragments arranged by size:
2815 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2450 2971 3651 3925 4072 4483 2450
Size: 521 680 274 147 411 3230
Fragments arranged by size:
3230 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1496 2686 3444 4023 4519 1496
Size: 1190 758 579 496 2240
Fragments arranged by size:
2240 1190 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2803 2849 4059 104
Size: 2699 46 1210 1308
Fragments arranged by size:
2699 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2275 3053 3243 4037 2275
Size: 778 190 794 3501
Fragments arranged by size:
3501 794 778 190
BsmI GAATG_Cn'
Cuts at: 1420 1420
Size: 5263
Bsp1286I G_dGCh'C
Cuts at: 2275 2886 2979 3053 3243 3305 4037 2275
Size: 611 93 74 190 62 732 3501
Fragments arranged by size:
3501 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4483 4483
Size: 5263
BspHI T'CATG_A
Cuts at: 4439 4439
Size: 5263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1689 2172 2311 2525 2827 3208 1689
Size: 483 139 214 302 381 3744
Fragments arranged by size:
3744 483 381 302 214 139
BsrBI CCG'CTC
Cuts at: 50 3553 3607 5093 5258 50
Size: 3503 54 1486 165 55
Fragments arranged by size:
3503 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 164 3173 164
Size: 3009 2254
BsrFI r'CCGG_y
Cuts at: 3259 3440 3651 3259
Size: 181 211 4871
Fragments arranged by size:
4871 211 181
BssSI C'ACGA_G
Cuts at: 3532 3892 3532
Size: 360 4903
BstAPI GCAn_nnn'nTGC
Cuts at: 2638 2638
Size: 5263
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5263
BstXI CCAn_nnnn'nTGG
Cuts at: 172 369 172
Size: 197 5066
BstYI r'GATC_y
Cuts at: 1 1254 1833 2286 2753 2776 3111 3357 4371
Size: 1253 579 453 467 23 335 246 1014
Cuts at: 4371 4457 4469 4815 1
Size: 86 12 346 449
Fragments arranged by size:
1253 1014 579 467 453 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 354 519 354
Size: 165 5098
BtgI C'CryG_G
Cuts at: 1041 1041
Size: 5263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3211 3392 3555 3211
Size: 181 163 4919
Fragments arranged by size:
4919 181 163
BtsI GCAGTG_nn'
Cuts at: 1425 1557 1668 1925 2000 2046 2504 1425
Size: 132 111 257 75 46 458 4184
Fragments arranged by size:
4184 458 257 132 111 75 46
ClaI AT'CG_AT
Cuts at: 1452 1452
Size: 5263
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1085 1120 1085
Size: 35 5228
DraI TTT'AAA
Cuts at: 261 2260 261
Size: 1999 3264
DrdI GACnn_nn'nnGTC
Cuts at: 2967 3827 2967
Size: 860 4403
EaeI y'GGCC_r
Cuts at: 1934 2846 3020 3411 3438 4785 1934
Size: 912 174 391 27 1347 2412
Fragments arranged by size:
2412 1347 912 391 174 27
EagI C'GGCC_G
Cuts at: 2846 2846
Size: 5263
EarI CTCTTCn'nnn_
Cuts at: 812 1589 1607 3284 3494 812
Size: 777 18 1677 210 2581
Fragments arranged by size:
2581 1677 777 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3469 3781 3927 5073 3469
Size: 312 146 1146 3659
Fragments arranged by size:
3659 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1570 1580 1728 1753 1835 3086 3518 4267
Size: 575 10 148 25 82 1251 432 749
Cuts at: 4267 4535 995
Size: 268 1723
Fragments arranged by size:
1723 1251 749 575 432 268 148 82
25 10
EcoO109I rG'GnC_Cy
Cuts at: 2400 2400
Size: 5263
EcoRI G'AATT_C
Cuts at: 2265 2265
Size: 5263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 263 3280 263
Size: 3017 2246
FspI TGC'GCA
Cuts at: 3042 3042
Size: 5263
HaeII r_GCGC'y
Cuts at: 2695 2943 3967 4946 2695
Size: 248 1024 979 3012
Fragments arranged by size:
3012 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 1465 1497 3103 3133 3135 3165 154
Size: 32 1279 32 1606 30 2 30 2252
Fragments arranged by size:
2252 1606 1279 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2300 2300
Size: 5263
HindIII A'AGCT_T
Cuts at: 288 288
Size: 5263
KasI G'GCGC_C
Cuts at: 2691 2939 2691
Size: 248 5015
KpnI G_GTAC'C
Cuts at: 2281 2281
Size: 5263
MluI A'CGCG_T
Cuts at: 4696 4696
Size: 5263
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 716 1694 3934 4118 302
Size: 414 978 2240 184 1447
Fragments arranged by size:
2240 1447 978 414 184
MscI TGG'CCA
Cuts at: 1936 3022 1936
Size: 1086 4177
MslI CAynn'nnrTG
Cuts at: 248 497 3250 3377 248
Size: 249 2753 127 2134
Fragments arranged by size:
2753 2134 249 127
MspA1I CmG'CkG
Cuts at: 1330 2372 2686 3046 4061 4306 1330
Size: 1042 314 360 1015 245 2287
Fragments arranged by size:
2287 1042 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3442 3442
Size: 5263
NarI GG'CG_CC
Cuts at: 2692 2940 2692
Size: 248 5015
NcoI C'CATG_G
Cuts at: 1041 1041
Size: 5263
NdeI CA'TA_TG
Cuts at: 1827 1827
Size: 5263
NgoMIV G'CCGG_C
Cuts at: 3440 3440
Size: 5263
NruI TCG'CGA
Cuts at: 3624 3624
Size: 5263
NspI r_CATG'y
Cuts at: 247 2138 2314 2814 3723 247
Size: 1891 176 500 909 1787
Fragments arranged by size:
1891 1787 909 500 176
PciI A'CATG_T
Cuts at: 3719 3719
Size: 5263
PflMI CCAn_nnn'nTGG
Cuts at: 1884 1884
Size: 5263
PfoI T'CCnGG_A
Cuts at: 567 567
Size: 5263
PmeI GTTT'AAAC
Cuts at: 2260 2260
Size: 5263
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4402 4434 4402
Size: 32 5231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1520 1552 1520
Size: 32 5231
PstI C_TGCA'G
Cuts at: 681 2187 2308 2993 681
Size: 1506 121 685 2951
Fragments arranged by size:
2951 1506 685 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5263
PvuII CAG'CTG
Cuts at: 2686 3046 2686
Size: 360 4903
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 5263
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5263
SalI G'TCGA_C
Cuts at: 2298 2298
Size: 5263
SapI GCTCTTCn'nnn_
Cuts at: 3284 3494 3284
Size: 210 5053
SbfI CC_TGCA'GG
Cuts at: 2308 2308
Size: 5263
ScaI AGT'ACT
Cuts at: 4780 4780
Size: 5263
SfcI C'TryA_G
Cuts at: 33 677 2183 2304 2989 3984 4175 33
Size: 644 1506 121 685 995 191 1121
Fragments arranged by size:
1506 1121 995 685 644 191 121
SfoI GGC'GCC
Cuts at: 2693 2941 2693
Size: 248 5015
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5263
SmaI CCC'GGG
Cuts at: 2283 2283
Size: 5263
SmlI C'TyrA_G
Cuts at: 905 1266 1482 2416 3331 3825 4087 905
Size: 361 216 934 915 494 262 2081
Fragments arranged by size:
2081 934 915 494 361 262 216
SnaBI TAC'GTA
Cuts at: 2253 2253
Size: 5263
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5263
SphI G_CATG'C
Cuts at: 2314 2814 2314
Size: 500 4763
StyI C'CwwG_G
Cuts at: 1041 1607 1627 2395 2749 1041
Size: 566 20 768 354 3555
Fragments arranged by size:
3555 768 566 354 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2844 2977 3436 3647 2844
Size: 133 459 211 4460
Fragments arranged by size:
4460 459 211 133
TatI w'GTAC_w
Cuts at: 134 489 529 1637 4778 134
Size: 355 40 1108 3141 619
Fragments arranged by size:
3141 1108 619 355 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1353 1385 4780 4812 1353
Size: 32 3395 32 1804
Fragments arranged by size:
3395 1804 32 32
Tth111I GACn'n_nGTC
Cuts at: 3058 3058
Size: 5263
XbaI T'CTAG_A
Cuts at: 2292 2292
Size: 5263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 2756
Size: 5263
XmaI C'CCGG_G
Cuts at: 2281 2281
Size: 5263
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PciI PflMI
PfoI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI