Restriction map for
pF1KSDA0441
(Circular) (Six-base) MAPSORT of: pF1KSDA0441.seq Check: 453 from: 1 to: 5228
pF1KSDA0441 5228 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1186 2144 1186
Size: 958 4270
AccI GT'mk_AC
Cuts at: 2266 2266
Size: 5228
Acc65I G'GTAC_C
Cuts at: 2244 2244
Size: 5228
AclI AA'CG_TT
Cuts at: 5068 5068
Size: 5228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 1664 1720 2102 3053 3485 4232 4500 1456
Size: 208 56 382 951 432 747 268 2184
Fragments arranged by size:
2184 951 747 432 382 268 208 56
AflIII A'CryG_T
Cuts at: 2580 3684 4661 2580
Size: 1104 977 3147
Fragments arranged by size:
3147 1104 977
AgeI A'CCGG_T
Cuts at: 3616 3616
Size: 5228
AleI CACnn'nnGTG
Cuts at: 1412 1412
Size: 5228
AlwNI CAG_nnn'CTG
Cuts at: 2136 2533 4100 2136
Size: 397 1567 3264
Fragments arranged by size:
3264 1567 397
ApaI G_GGCC'C
Cuts at: 276 2012 276
Size: 1736 3492
ApaLI G'TGCA_C
Cuts at: 2029 3998 2029
Size: 1969 3259
ApoI r'AATT_y
Cuts at: 400 1366 1655 1778 1935 2232 4581 4837 400
Size: 966 289 123 157 297 2349 256 791
Fragments arranged by size:
2349 966 791 297 289 256 157 123
AseI AT'TA_AT
Cuts at: 20 20
Size: 5228
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
AvaI C'yCGr_G
Cuts at: 1764 2248 1764
Size: 484 4744
BamHI G'GATC_C
Cuts at: 2253 2253
Size: 5228
BanI G'GyrC_C
Cuts at: 556 2244 2658 2906 2941 556
Size: 1688 414 248 35 2843
Fragments arranged by size:
2843 1688 414 248 35
BanII G_rGCy'C
Cuts at: 276 2012 2099 2242 3272 276
Size: 1736 87 143 1030 2232
Fragments arranged by size:
2232 1736 1030 143 87
BbeI G_GCGC'C
Cuts at: 2662 2910 2662
Size: 248 4980
BbsI GAAGACnn'nnnn_
Cuts at: 621 621
Size: 5228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 119 153 119
Size: 34 5194
BciVI GTATCCnnnnn_n'
Cuts at: 400 3126 3887 5228 400
Size: 2726 761 1341 400
Fragments arranged by size:
2726 1341 761 400
BclI T'GATC_A
Cuts at: 1266 2036 2748 1266
Size: 770 712 3746
Fragments arranged by size:
3746 770 712
BglI GCCn_nnn'nGGC
Cuts at: 153 995 153
Size: 842 4386
BglII A'GATC_T
Cuts at: 2743 4780 2743
Size: 2037 3191
BlpI GC'TnA_GC
Cuts at: 2129 2340 2129
Size: 211 5017
Bme1580I G_kGCm'C
Cuts at: 276 561 2012 2033 2186 2853 2946 4002 276
Size: 285 1451 21 153 667 93 1056 1502
Fragments arranged by size:
1502 1451 1056 667 285 153 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 556 2440 2521 2856 5000 556
Size: 1884 81 335 2144 784
Fragments arranged by size:
2144 1884 784 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3595 3595
Size: 5228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1243 1530 1013
Size: 230 287 4711
Fragments arranged by size:
4711 287 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2404 3283 3775 4073 2404
Size: 879 492 298 3559
Fragments arranged by size:
3559 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4932 4932
Size: 5228
BsaAI yAC'GTr
Cuts at: 2034 2181 2220 2581 2034
Size: 147 39 361 4681
Fragments arranged by size:
4681 361 147 39
BsaBI GATnn'nnATC
Cuts at: 6 2742 4779 6
Size: 2736 2037 455
Fragments arranged by size:
2736 2037 455
BsaHI Gr'CG_yC
Cuts at: 2659 2907 3609 5105 2659
Size: 248 702 1496 2782
Fragments arranged by size:
2782 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1341 2417 2938 3616 3890 4037 4448 1341
Size: 1076 521 678 274 147 411 2121
Fragments arranged by size:
2121 1076 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 658 823 1080 1511 2107 658
Size: 165 257 431 596 3779
Fragments arranged by size:
3779 596 431 257 165
BseYI C'CCAG_C
Cuts at: 2186 2653 3411 3988 4484 2186
Size: 467 758 577 496 2930
Fragments arranged by size:
2930 758 577 496 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1164 1209 1222 1164
Size: 45 13 5170
Fragments arranged by size:
5170 45 13
BsiEI CG_ry'CG
Cuts at: 104 1171 2770 2816 4024 104
Size: 1067 1599 46 1208 1308
Fragments arranged by size:
1599 1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 1229 2033 2099 2242 3020 3210 4002 1229
Size: 804 66 143 778 190 792 2455
Fragments arranged by size:
2455 804 792 778 190 143 66
BsmI GAATG_Cn'
Cuts at: 1351 1568 1351
Size: 217 5011
Bsp1286I G_dGCh'C
Cuts at: 276 561 1229 2012 2033 2099 2186 2242 2853
Size: 285 668 783 21 66 87 56 611
Cuts at: 2853 2946 3020 3210 3272 4002 276
Size: 93 74 190 62 730 1502
Fragments arranged by size:
1502 783 730 668 611 285 190 93
87 74 66 62 56 21
BspEI T'CCGG_A
Cuts at: 4448 4448
Size: 5228
BspHI T'CATG_A
Cuts at: 4404 4404
Size: 5228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1186 2144 2278 2492 2794 3175 1186
Size: 958 134 214 302 381 3239
Fragments arranged by size:
3239 958 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 1167 3520 3574 5058 5223 50
Size: 1117 2353 54 1484 165 55
Fragments arranged by size:
2353 1484 1117 165 55 54
BsrDI GCAATG_nn'
Cuts at: 634 3140 634
Size: 2506 2722
BsrFI r'CCGG_y
Cuts at: 3226 3407 3616 3226
Size: 181 209 4838
Fragments arranged by size:
4838 209 181
BsrGI T'GTAC_A
Cuts at: 165 261 1803 165
Size: 96 1542 3590
Fragments arranged by size:
3590 1542 96
BssSI C'ACGA_G
Cuts at: 3499 3857 3499
Size: 358 4870
BstAPI GCAn_nnn'nTGC
Cuts at: 1187 1218 1355 2605 1187
Size: 31 137 1250 3810
Fragments arranged by size:
3810 1250 137 31
BstBI TT'CG_AA
Cuts at: 110 3589 110
Size: 3479 1749
BstYI r'GATC_y
Cuts at: 1 904 968 2121 2253 2720 2743 3078 3324
Size: 903 64 1153 132 467 23 335 246
Cuts at: 3324 4336 4422 4434 4780 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1153 1012 903 467 449 346 335 246
132 86 64 23 12
Bsu36I CC'TnA_GG
Cuts at: 1001 1001
Size: 5228
BtgI C'CryG_G
Cuts at: 125 951 125
Size: 826 4402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 122 1345 3178 3359 3522 122
Size: 1223 1833 181 163 1828
Fragments arranged by size:
1833 1828 1223 181 163
BtsI GCAGTG_nn'
Cuts at: 548 1216 1563 2471 548
Size: 668 347 908 3305
Fragments arranged by size:
3305 908 668 347
DraI TTT'AAA
Cuts at: 2227 2227
Size: 5228
DraIII CAC_nnn'GTG
Cuts at: 1187 2181 1187
Size: 994 4234
DrdI GACnn_nn'nnGTC
Cuts at: 2934 3792 2934
Size: 858 4370
EaeI y'GGCC_r
Cuts at: 197 342 861 1168 2202 2813 2987 3378 3405
Size: 145 519 307 1034 611 174 391 27
Cuts at: 3405 4750 197
Size: 1345 675
Fragments arranged by size:
1345 1034 675 611 519 391 307 174
145 27
EagI C'GGCC_G
Cuts at: 1168 2813 1168
Size: 1645 3583
EarI CTCTTCn'nnn_
Cuts at: 323 1475 1712 3251 3461 323
Size: 1152 237 1539 210 2090
Fragments arranged by size:
2090 1539 1152 237 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2150 3436 3746 3892 5038 2150
Size: 1286 310 146 1146 2340
Fragments arranged by size:
2340 1286 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2097 2240 2097
Size: 143 5085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1243 1456 1530 1664 1720 2102 3053 3485
Size: 230 213 74 134 56 382 951 432
Cuts at: 3485 4232 4500 1013
Size: 747 268 1741
Fragments arranged by size:
1741 951 747 432 382 268 230 213
134 74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 490 490
Size: 5228
EcoO109I rG'GnC_Cy
Cuts at: 997 2008 2009 2367 997
Size: 1011 1 358 3858
Fragments arranged by size:
3858 1011 358 1
EcoRI G'AATT_C
Cuts at: 2232 2232
Size: 5228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 304 1665 3247 243
Size: 61 1361 1582 2224
Fragments arranged by size:
2224 1582 1361 61
FspI TGC'GCA
Cuts at: 3009 3009
Size: 5228
HaeII r_GCGC'y
Cuts at: 990 2662 2910 3932 4911 990
Size: 1672 248 1022 979 1307
Fragments arranged by size:
1672 1307 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 634 666 3070 3100 3102 3132 634
Size: 32 2404 30 2 30 2730
Fragments arranged by size:
2730 2404 32 30 30 2
HincII GTy'rAC
Cuts at: 2267 2267
Size: 5228
KasI G'GCGC_C
Cuts at: 2658 2906 2658
Size: 248 4980
KpnI G_GTAC'C
Cuts at: 2248 2248
Size: 5228
MluI A'CGCG_T
Cuts at: 4661 4661
Size: 5228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3899 4083 3899
Size: 184 5044
MscI TGG'CCA
Cuts at: 199 863 2204 2989 199
Size: 664 1341 785 2438
Fragments arranged by size:
2438 1341 785 664
MslI CAynn'nnrTG
Cuts at: 355 428 1150 1375 1412 2069 3217 3344 355
Size: 73 722 225 37 657 1148 127 2239
Fragments arranged by size:
2239 1148 722 657 225 127 73 37
MspA1I CmG'CkG
Cuts at: 1010 2142 2339 2653 3013 4026 4271 1010
Size: 1132 197 314 360 1013 245 1967
Fragments arranged by size:
1967 1132 1013 360 314 245 197
NaeI GCC'GGC
Cuts at: 3409 3409
Size: 5228
NarI GG'CG_CC
Cuts at: 2659 2907 2659
Size: 248 4980
NcoI C'CATG_G
Cuts at: 125 951 125
Size: 826 4402
NgoMIV G'CCGG_C
Cuts at: 3407 3407
Size: 5228
NspI r_CATG'y
Cuts at: 1678 2281 2781 3688 1678
Size: 603 500 907 3218
Fragments arranged by size:
3218 907 603 500
PciI A'CATG_T
Cuts at: 3684 3684
Size: 5228
PmeI GTTT'AAAC
Cuts at: 2227 2227
Size: 5228
PmlI CAC'GTG
Cuts at: 2034 2181 2034
Size: 147 5081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 5196
PshAI GACnn'nnGTC
Cuts at: 1251 1251
Size: 5228
PspOMI G'GGCC_C
Cuts at: 272 2008 272
Size: 1736 3492
PstI C_TGCA'G
Cuts at: 666 1860 1884 1934 2141 2275 2960 666
Size: 1194 24 50 207 134 685 2934
Fragments arranged by size:
2934 1194 685 207 134 50 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5228
PvuII CAG'CTG
Cuts at: 2142 2653 3013 2142
Size: 511 360 4357
Fragments arranged by size:
4357 511 360
RsrII CG'GwC_CG
Cuts at: 3423 3423
Size: 5228
SacI G_AGCT'C
Cuts at: 2099 2242 2099
Size: 143 5085
SalI G'TCGA_C
Cuts at: 2265 2265
Size: 5228
SapI GCTCTTCn'nnn_
Cuts at: 3251 3461 3251
Size: 210 5018
SbfI CC_TGCA'GG
Cuts at: 2275 2275
Size: 5228
ScaI AGT'ACT
Cuts at: 2115 4745 2115
Size: 2630 2598
SexAI A'CCwGG_T
Cuts at: 485 485
Size: 5228
SfcI C'TryA_G
Cuts at: 33 662 1135 1741 1856 1880 1930 2137 2271
Size: 629 473 606 115 24 50 207 134
Cuts at: 2271 2956 3949 4140 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 629 606 473 207 191
134 115 50 24
SfoI GGC'GCC
Cuts at: 2660 2908 2660
Size: 248 4980
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5228
SmaI CCC'GGG
Cuts at: 2250 2250
Size: 5228
SmlI C'TyrA_G
Cuts at: 2383 3298 3790 4052 2383
Size: 915 492 262 3559
Fragments arranged by size:
3559 915 492 262
SnaBI TAC'GTA
Cuts at: 2220 2220
Size: 5228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5228
SphI G_CATG'C
Cuts at: 1678 2281 2781 1678
Size: 603 500 4125
Fragments arranged by size:
4125 603 500
SspI AAT'ATT
Cuts at: 1282 1731 1282
Size: 449 4779
StyI C'CwwG_G
Cuts at: 125 951 2048 2362 2716 125
Size: 826 1097 314 354 2637
Fragments arranged by size:
2637 1097 826 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 868 2184 2811 2944 3403 3612 868
Size: 1316 627 133 459 209 2484
Fragments arranged by size:
2484 1316 627 459 209 133
TatI w'GTAC_w
Cuts at: 165 261 439 741 1037 1803 2113 4743 165
Size: 96 178 302 296 766 310 2630 650
Fragments arranged by size:
2630 766 650 310 302 296 178 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4745 4777 4745
Size: 32 5196
Tth111I GACn'n_nGTC
Cuts at: 3025 3025
Size: 5228
XbaI T'CTAG_A
Cuts at: 2259 2259
Size: 5228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 357 2055 2723 357
Size: 1698 668 2862
Fragments arranged by size:
2862 1698 668
XmaI C'CCGG_G
Cuts at: 2248 2248
Size: 5228
XmnI GAAnn'nnTTC
Cuts at: 226 1591 1973 2066 226
Size: 1365 382 93 3388
Fragments arranged by size:
3388 1365 382 93
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI