Restriction map for
pF1KSDA0463
(Circular) (Six-base) MAPSORT of: pF1KSDA0463.seq Check: 153 from: 1 to: 8864
pF1KSDA0463 8864 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1974 2239 2651 2844 3466 1974
Size: 265 412 193 622 7372
Fragments arranged by size:
7372 622 412 265 193
AatII G_ACGT'C
Cuts at: 1343 5020 1343
Size: 3677 5187
AccI GT'mk_AC
Cuts at: 929 1711 3131 3242 5050 5902 929
Size: 782 1420 111 1808 852 3891
Fragments arranged by size:
3891 1808 1420 852 782 111
Acc65I G'GTAC_C
Cuts at: 5880 5880
Size: 8864
AclI AA'CG_TT
Cuts at: 8704 8704
Size: 8864
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 3682 4246 5028 6689 7121 7868 8136 842
Size: 2840 564 782 1661 432 747 268 1570
Fragments arranged by size:
2840 1661 1570 782 747 564 432 268
AfeI AGC'GCT
Cuts at: 1648 5292 1648
Size: 3644 5220
AflII C'TTAA_G
Cuts at: 2335 2335
Size: 8864
AflIII A'CryG_T
Cuts at: 1049 1775 5315 6216 7320 8297 1049
Size: 726 3540 901 1104 977 1616
Fragments arranged by size:
3540 1616 1104 977 901 726
AgeI A'CCGG_T
Cuts at: 7252 7252
Size: 8864
AhdI GACnn_n'nnGTC
Cuts at: 444 792 444
Size: 348 8516
AleI CACnn'nnGTG
Cuts at: 730 3022 3082 5368 730
Size: 2292 60 2286 4226
Fragments arranged by size:
4226 2292 2286 60
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 502 604 502
Size: 102 8762
AlwNI CAG_nnn'CTG
Cuts at: 1407 1885 2559 2702 4465 5805 6169 7736 1407
Size: 478 674 143 1763 1340 364 1567 2535
Fragments arranged by size:
2535 1763 1567 1340 674 478 364 143
ApaI G_GGCC'C
Cuts at: 201 2024 4588 5085 5772 201
Size: 1823 2564 497 687 3293
Fragments arranged by size:
3293 2564 1823 687 497
ApaLI G'TGCA_C
Cuts at: 1347 2664 5278 7634 1347
Size: 1317 2614 2356 2577
Fragments arranged by size:
2614 2577 2356 1317
ApoI r'AATT_y
Cuts at: 3400 5868 8217 8473 3400
Size: 2468 2349 256 3791
Fragments arranged by size:
3791 2468 2349 256
AseI AT'TA_AT
Cuts at: 20 5831 20
Size: 5811 3053
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8864
AvaI C'yCGr_G
Cuts at: 194 782 949 1073 3064 3293 3962 4149 4853
Size: 588 167 124 1991 229 669 187 704
Cuts at: 4853 5078 5884 194
Size: 225 806 3174
Fragments arranged by size:
3174 1991 806 704 669 588 229 225
187 167 124
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4107 4140 4107
Size: 33 8831
BamHI G'GATC_C
Cuts at: 3389 5889 3389
Size: 2500 6364
BanI G'GyrC_C
Cuts at: 667 1277 1356 2283 2865 3465 4835 5227 5880
Size: 610 79 927 582 600 1370 392 653
Cuts at: 5880 6294 6542 6577 667
Size: 414 248 35 2954
Fragments arranged by size:
2954 1370 927 653 610 600 582 414
392 248 79 35
BanII G_rGCy'C
Cuts at: 201 1319 1738 2024 3252 4058 4588 5085 5762
Size: 1118 419 286 1228 806 530 497 677
Cuts at: 5762 5772 5878 6908 201
Size: 10 106 1030 2157
Fragments arranged by size:
2157 1228 1118 1030 806 677 530 497
419 286 106 10
BbeI G_GCGC'C
Cuts at: 1281 1360 4839 6298 6546 1281
Size: 79 3479 1459 248 3599
Fragments arranged by size:
3599 3479 1459 248 79
BbvCI CC'TCA_GC
Cuts at: 798 2080 798
Size: 1282 7582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1210 1244 1210
Size: 34 8830
BciVI GTATCCnnnnn_n'
Cuts at: 6762 7523 8864 6762
Size: 761 1341 6762
Fragments arranged by size:
6762 1341 761
BclI T'GATC_A
Cuts at: 1641 5090 6384 1641
Size: 3449 1294 4121
Fragments arranged by size:
4121 3449 1294
BfrBI ATG'CAT
Cuts at: 4926 4926
Size: 8864
BglI GCCn_nnn'nGGC
Cuts at: 2375 4355 2375
Size: 1980 6884
BglII A'GATC_T
Cuts at: 2297 5195 5726 6379 8416 2297
Size: 2898 531 653 2037 2745
Fragments arranged by size:
2898 2745 2037 653 531
BlpI GC'TnA_GC
Cuts at: 1903 2903 3613 4052 5976 1903
Size: 1000 710 439 1924 4791
Fragments arranged by size:
4791 1924 1000 710 439
Bme1580I G_kGCm'C
Cuts at: 201 670 1351 1767 2024 2145 2288 2668 2870
Size: 469 681 416 257 121 143 380 202
Cuts at: 2870 4588 4793 5085 5230 5282 5718 5772 6489
Size: 1718 205 292 145 52 436 54 717
Cuts at: 6489 6582 7638 201
Size: 93 1056 1427
Fragments arranged by size:
1718 1427 1056 717 681 469 436 416
380 292 257 205 202 145 143 121
93 54 52
BmrI ACTGGGnnnn_n'
Cuts at: 4711 5430 6076 6157 6492 8636 4711
Size: 719 646 81 335 2144 4939
Fragments arranged by size:
4939 2144 719 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1605 1940 5748 7231 1605
Size: 335 3808 1483 3238
Fragments arranged by size:
3808 3238 1483 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1004 1333 1406 2089 2427 3644 3713 4033
Size: 781 329 73 683 338 1217 69 320
Cuts at: 4033 4042 4204 4306 4441 223
Size: 9 162 102 135 4646
Fragments arranged by size:
4646 1217 781 683 338 329 320 162
135 102 73 69 9
Bpu10I CC'TnA_GC
Cuts at: 798 1732 2080 4098 4624 798
Size: 934 348 2018 526 5038
Fragments arranged by size:
5038 2018 934 526 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2599 3967 4369 6040 6919 7411 7709 1582
Size: 1017 1368 402 1671 879 492 298 2737
Fragments arranged by size:
2737 1671 1368 1017 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 779 882 946 980 1593 3724 3751 5254 8568
Size: 103 64 34 613 2131 27 1503 3314
Cuts at: 8568 779
Size: 1075
Fragments arranged by size:
3314 2131 1503 1075 613 103 64 34
27
BsaAI yAC'GTr
Cuts at: 1050 3816 4212 5856 6217 1050
Size: 2766 396 1644 361 3697
Fragments arranged by size:
3697 2766 1644 396 361
BsaBI GATnn'nnATC
Cuts at: 6 6378 8415 6
Size: 6372 2037 455
Fragments arranged by size:
6372 2037 455
BsaHI Gr'CG_yC
Cuts at: 1278 1340 1357 4836 5017 5474 6295 6543 7245
Size: 62 17 3479 181 457 821 248 702
Cuts at: 7245 8741 1278
Size: 1496 1401
Fragments arranged by size:
3479 1496 1401 821 702 457 248 181
62 17
BsaWI w'CCGG_w
Cuts at: 2624 4451 6053 6574 7252 7526 7673 8084 2624
Size: 1827 1602 521 678 274 147 411 3404
Fragments arranged by size:
3404 1827 1602 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1765 1795 5536 5566 1765
Size: 30 3741 30 5063
Fragments arranged by size:
5063 3741 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 828 2308 3904 3907 4968 5028 5770 139
Size: 689 1480 1596 3 1061 60 742 3233
Fragments arranged by size:
3233 1596 1480 1061 742 689 60 3
BseYI C'CCAG_C
Cuts at: 261 1329 1867 2276 2354 2588 2860 3112 3761
Size: 1068 538 409 78 234 272 252 649
Cuts at: 3761 4238 4799 5535 5584 5597 5634 6289 7047
Size: 477 561 736 49 13 37 655 758
Cuts at: 7047 7624 8120 261
Size: 577 496 1005
Fragments arranged by size:
1068 1005 758 736 655 649 577 561
538 496 477 409 272 252 234 78
49 37 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1053 1753 2196 2603 2667 2856 2872 4492
Size: 563 700 443 407 64 189 16 1620
Cuts at: 4492 5671 490
Size: 1179 3683
Fragments arranged by size:
3683 1620 1179 700 563 443 407 189
64 16
BsiEI CG_ry'CG
Cuts at: 104 2625 3246 3902 4317 4807 6406 6452 7660
Size: 2521 621 656 415 490 1599 46 1208
Cuts at: 7660 104
Size: 1308
Fragments arranged by size:
2521 1599 1308 1208 656 621 490 415
46
BsiHKAI G_wGCw'C
Cuts at: 133 491 641 1319 1351 1598 1738 1783 1892
Size: 358 150 678 32 247 140 45 109
Cuts at: 1892 2668 2737 3722 4058 5282 5531 5762 5878
Size: 776 69 985 336 1224 249 231 116
Cuts at: 5878 6656 6846 7638 133
Size: 778 190 792 1359
Fragments arranged by size:
1359 1224 985 792 778 776 678 358
336 249 247 231 190 150 140 116
109 69 45 32
BsmI GAATG_Cn'
Cuts at: 5664 5664
Size: 8864
BsmBI CGTCTCn'nnnn_
Cuts at: 151 151
Size: 8864
Bsp1286I G_dGCh'C
Cuts at: 133 201 491 641 670 1319 1351 1598 1738
Size: 68 290 150 29 649 32 247 140
Cuts at: 1738 1767 1783 1892 2024 2145 2288 2668 2737
Size: 29 16 109 132 121 143 380 69
Cuts at: 2737 2870 3252 3722 4058 4588 4793 5085 5230
Size: 133 382 470 336 530 205 292 145
Cuts at: 5230 5282 5531 5718 5762 5772 5878 6489 6582
Size: 52 249 187 44 10 106 611 93
Cuts at: 6582 6656 6846 6908 7638 133
Size: 74 190 62 730 1359
Fragments arranged by size:
1359 730 649 611 530 470 382 380
336 292 290 249 247 205 190 187
150 145 143 140 133 132 121 109
106 93 74 69 68 62 52 44
32 29 29 16 10
BspEI T'CCGG_A
Cuts at: 4451 8084 4451
Size: 3633 5231
BspHI T'CATG_A
Cuts at: 2217 2993 4340 8040 2217
Size: 776 1347 3700 3041
Fragments arranged by size:
3700 3041 1347 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1974 2239 2651 2844 3157 3275 3466 5914 6128
Size: 265 412 193 313 118 191 2448 214
Cuts at: 6128 6430 6811 1974
Size: 302 381 4027
Fragments arranged by size:
4027 2448 412 381 313 302 265 214
193 191 118
BsrBI CCG'CTC
Cuts at: 50 218 2425 4097 7156 7210 8694 8859 50
Size: 168 2207 1672 3059 54 1484 165 55
Fragments arranged by size:
3059 2207 1672 1484 168 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3323 3941 6776 3323
Size: 618 2835 5411
Fragments arranged by size:
5411 2835 618
BsrFI r'CCGG_y
Cuts at: 268 1895 3094 3901 4345 5531 6862 7043 7252
Size: 1627 1199 807 444 1186 1331 181 209
Cuts at: 7252 268
Size: 1880
Fragments arranged by size:
1880 1627 1331 1199 1186 807 444 209
181
BsrGI T'GTAC_A
Cuts at: 1442 1442
Size: 8864
BssSI C'ACGA_G
Cuts at: 2935 7135 7493 2935
Size: 4200 358 4306
Fragments arranged by size:
4306 4200 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2210 6241 2210
Size: 4031 4833
BstBI TT'CG_AA
Cuts at: 110 7225 110
Size: 7115 1749
BstEII G'GTnAC_C
Cuts at: 1677 3084 5152 5167 1677
Size: 1407 2068 15 5374
Fragments arranged by size:
5374 2068 1407 15
BstXI CCAn_nnnn'nTGG
Cuts at: 411 2479 3106 4089 411
Size: 2068 627 983 5186
Fragments arranged by size:
5186 2068 983 627
BstYI r'GATC_y
Cuts at: 1 851 2297 3389 4712 5195 5726 5889 6356
Size: 850 1446 1092 1323 483 531 163 467
Cuts at: 6356 6379 6714 6960 7972 8058 8070 8416 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1446 1323 1092 1012 850 531 483 467
449 346 335 246 163 86 23 12
BstZ17I GTA'TAC
Cuts at: 1712 5051 1712
Size: 3339 5525
Bsu36I CC'TnA_GG
Cuts at: 959 2552 959
Size: 1593 7271
BtgI C'CryG_G
Cuts at: 1563 1691 2504 2805 3479 5148 5693 1563
Size: 128 813 301 674 1669 545 4734
Fragments arranged by size:
4734 1669 813 674 545 301 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2883 3879 5607 6814 6995 7158 2883
Size: 996 1728 1207 181 163 4589
Fragments arranged by size:
4589 1728 1207 996 181 163
BtsI GCAGTG_nn'
Cuts at: 1865 2145 4820 6107 1865
Size: 280 2675 1287 4622
Fragments arranged by size:
4622 2675 1287 280
ClaI AT'CG_AT
Cuts at: 1370 1821 4409 1370
Size: 451 2588 5825
Fragments arranged by size:
5825 2588 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1406 1441 4872 4907 1406
Size: 35 3431 35 5363
Fragments arranged by size:
5363 3431 35 35
DraI TTT'AAA
Cuts at: 5863 5863
Size: 8864
DraIII CAC_nnn'GTG
Cuts at: 2850 5694 2850
Size: 2844 6020
DrdI GACnn_nn'nnGTC
Cuts at: 2563 3295 4168 6570 7428 2563
Size: 732 873 2402 858 3999
Fragments arranged by size:
3999 2402 873 858 732
EaeI y'GGCC_r
Cuts at: 968 1115 2367 2501 3096 3333 3899 4804 5216
Size: 147 1252 134 595 237 566 905 412
Cuts at: 5216 5303 6449 6623 7014 7041 8386 968
Size: 87 1146 174 391 27 1345 1446
Fragments arranged by size:
1446 1345 1252 1146 905 595 566 412
391 237 174 147 134 87 27
EagI C'GGCC_G
Cuts at: 3899 4804 6449 3899
Size: 905 1645 6314
Fragments arranged by size:
6314 1645 905
EarI CTCTTCn'nnn_
Cuts at: 428 610 721 997 4549 6887 7097 428
Size: 182 111 276 3552 2338 210 2195
Fragments arranged by size:
3552 2338 2195 276 210 182 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7072 7382 7528 8674 7072
Size: 310 146 1146 7262
Fragments arranged by size:
7262 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1317 1736 4056 5760 5876 1317
Size: 419 2320 1704 116 4305
Fragments arranged by size:
4305 2320 1704 419 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 842 1004 1333 1406 2089 2427 3644 3682
Size: 619 162 329 73 683 338 1217 38
Cuts at: 3682 3713 4033 4042 4204 4246 4306 4441 5028
Size: 31 320 9 162 42 60 135 587
Cuts at: 5028 6689 7121 7868 8136 223
Size: 1661 432 747 268 951
Fragments arranged by size:
1661 1217 951 747 683 619 587 432
338 329 320 268 162 162 135 73
60 42 38 31 9
EcoNI CCTnn'n_nnAGG
Cuts at: 568 1099 1969 4627 5043 5776 568
Size: 531 870 2658 416 733 3656
Fragments arranged by size:
3656 2658 870 733 531 416
EcoO109I rG'GnC_Cy
Cuts at: 198 1431 1686 1743 2418 2556 3061 4221 4584
Size: 1233 255 57 675 138 505 1160 363
Cuts at: 4584 4585 5082 5768 5769 6003 198
Size: 1 497 686 1 234 3059
Fragments arranged by size:
3059 1233 1160 686 675 505 497 363
255 234 138 57 1 1
EcoRI G'AATT_C
Cuts at: 5868 5868
Size: 8864
EcoRV GAT'ATC
Cuts at: 4068 4068
Size: 8864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6883 6883
Size: 8864
FspI TGC'GCA
Cuts at: 4530 6645 4530
Size: 2115 6749
HaeII r_GCGC'y
Cuts at: 1281 1360 1650 2177 4839 5294 6298 6546 7568
Size: 79 290 527 2662 455 1004 248 1022
Cuts at: 7568 8547 1281
Size: 979 1598
Fragments arranged by size:
2662 1598 1022 1004 979 527 455 290
248 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 430 462 778 810 820 852 1379
Size: 32 196 32 316 32 10 32 527
Cuts at: 1379 1411 2786 2818 3638 3670 3993 4025 4914
Size: 32 1375 32 820 32 323 32 889
Cuts at: 4914 4946 5536 5568 6706 6736 6738 6768 202
Size: 32 590 32 1138 30 2 30 2298
Fragments arranged by size:
2298 1375 1138 889 820 590 527 323
316 196 32 32 32 32 32 32
32 32 32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 510 657 2169 3243 4079 4686 5903 510
Size: 147 1512 1074 836 607 1217 3471
Fragments arranged by size:
3471 1512 1217 1074 836 607 147
HindIII A'AGCT_T
Cuts at: 4043 4043
Size: 8864
KasI G'GCGC_C
Cuts at: 1277 1356 4835 6294 6542 1277
Size: 79 3479 1459 248 3599
Fragments arranged by size:
3599 3479 1459 248 79
KpnI G_GTAC'C
Cuts at: 5884 5884
Size: 8864
MfeI C'AATT_G
Cuts at: 4488 4488
Size: 8864
MluI A'CGCG_T
Cuts at: 8297 8297
Size: 8864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 5579 7535 7719 2762
Size: 2817 1956 184 3907
Fragments arranged by size:
3907 2817 1956 184
MscI TGG'CCA
Cuts at: 970 1117 3335 5218 5305 6625 970
Size: 147 2218 1883 87 1320 3209
Fragments arranged by size:
3209 2218 1883 1320 147 87
MslI CAynn'nnrTG
Cuts at: 409 613 730 3022 3082 4921 5368 6853 6980
Size: 204 117 2292 60 1839 447 1485 127
Cuts at: 6980 409
Size: 2293
Fragments arranged by size:
2293 2292 1839 1485 447 204 127 117
60
MspA1I CmG'CkG
Cuts at: 982 2046 2079 2183 2280 2592 2651 4392 4803
Size: 1064 33 104 97 312 59 1741 411
Cuts at: 4803 5802 5975 6289 6649 7662 7907 982
Size: 999 173 314 360 1013 245 1939
Fragments arranged by size:
1939 1741 1064 1013 999 411 360 314
312 245 173 104 97 59 33
NaeI GCC'GGC
Cuts at: 270 3903 7045 270
Size: 3633 3142 2089
Fragments arranged by size:
3633 3142 2089
NarI GG'CG_CC
Cuts at: 1278 1357 4836 6295 6543 1278
Size: 79 3479 1459 248 3599
Fragments arranged by size:
3599 3479 1459 248 79
NcoI C'CATG_G
Cuts at: 1563 2805 1563
Size: 1242 7622
NgoMIV G'CCGG_C
Cuts at: 268 3901 7043 268
Size: 3633 3142 2089
Fragments arranged by size:
3633 3142 2089
NsiI A_TGCA'T
Cuts at: 4928 4928
Size: 8864
NspI r_CATG'y
Cuts at: 276 1779 5319 5709 5917 6417 7324 276
Size: 1503 3540 390 208 500 907 1816
Fragments arranged by size:
3540 1816 1503 907 500 390 208
PasI CC'CwG_GG
Cuts at: 1407 2884 1407
Size: 1477 7387
PciI A'CATG_T
Cuts at: 1775 5315 7320 1775
Size: 3540 2005 3319
Fragments arranged by size:
3540 3319 2005
PflMI CCAn_nnn'nTGG
Cuts at: 1407 2702 1407
Size: 1295 7569
PfoI T'CCnGG_A
Cuts at: 1619 1990 2240 5011 1619
Size: 371 250 2771 5472
Fragments arranged by size:
5472 2771 371 250
PmeI GTTT'AAAC
Cuts at: 5863 5863
Size: 8864
PmlI CAC'GTG
Cuts at: 3816 4212 3816
Size: 396 8468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5251 5283 8003 8035 5251
Size: 32 2720 32 6080
Fragments arranged by size:
6080 2720 32 32
PpuMI rG'GwC_Cy
Cuts at: 1686 1743 2418 2556 3061 1686
Size: 57 675 138 505 7489
Fragments arranged by size:
7489 675 505 138 57
PsiI TTA'TAA
Cuts at: 5813 5813
Size: 8864
PspOMI G'GGCC_C
Cuts at: 197 2020 4584 5081 5768 197
Size: 1823 2564 497 687 3293
Fragments arranged by size:
3293 2564 1823 687 497
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2452 2484 2452
Size: 32 8832
PstI C_TGCA'G
Cuts at: 481 1103 2140 3272 4295 4355 5237 5911 6596
Size: 622 1037 1132 1023 60 882 674 685
Cuts at: 6596 481
Size: 2749
Fragments arranged by size:
2749 1132 1037 1023 882 685 674 622
60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8864
PvuII CAG'CTG
Cuts at: 2079 2280 4392 6289 6649 2079
Size: 201 2112 1897 360 4294
Fragments arranged by size:
4294 2112 1897 360 201
RsrII CG'GwC_CG
Cuts at: 7059 7059
Size: 8864
SacI G_AGCT'C
Cuts at: 1319 1738 4058 5762 5878 1319
Size: 419 2320 1704 116 4305
Fragments arranged by size:
4305 2320 1704 419 116
SalI G'TCGA_C
Cuts at: 3241 5901 3241
Size: 2660 6204
SanDI GG'GwC_CC
Cuts at: 1686 1743 2418 1686
Size: 57 675 8132
Fragments arranged by size:
8132 675 57
SapI GCTCTTCn'nnn_
Cuts at: 721 6887 7097 721
Size: 6166 210 2488
Fragments arranged by size:
6166 2488 210
SbfI CC_TGCA'GG
Cuts at: 1103 4355 5911 1103
Size: 3252 1556 4056
Fragments arranged by size:
4056 3252 1556
ScaI AGT'ACT
Cuts at: 8381 8381
Size: 8864
SfcI C'TryA_G
Cuts at: 33 477 1099 1282 1497 2074 2136 3268 4291
Size: 444 622 183 215 577 62 1132 1023
Cuts at: 4291 4351 5233 5907 6592 7585 7776 33
Size: 60 882 674 685 993 191 1121
Fragments arranged by size:
1132 1121 1023 993 882 685 674 622
577 444 215 191 183 62 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 4355 4355
Size: 8864
SfoI GGC'GCC
Cuts at: 1279 1358 4837 6296 6544 1279
Size: 79 3479 1459 248 3599
Fragments arranged by size:
3599 3479 1459 248 79
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8864
SmaI CCC'GGG
Cuts at: 196 1075 4151 4855 5080 5886 196
Size: 879 3076 704 225 806 3174
Fragments arranged by size:
3174 3076 879 806 704 225
SmlI C'TyrA_G
Cuts at: 1597 2335 2614 3962 3982 4384 6019 6934 7426
Size: 738 279 1348 20 402 1635 915 492
Cuts at: 7426 7688 1597
Size: 262 2773
Fragments arranged by size:
2773 1635 1348 915 738 492 402 279
262 20
SnaBI TAC'GTA
Cuts at: 5856 5856
Size: 8864
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8864
SphI G_CATG'C
Cuts at: 276 5917 6417 276
Size: 5641 500 2723
Fragments arranged by size:
5641 2723 500
SspI AAT'ATT
Cuts at: 2259 2259
Size: 8864
StuI AGG'CCT
Cuts at: 1144 1807 1144
Size: 663 8201
StyI C'CwwG_G
Cuts at: 339 1352 1563 2024 2087 2805 3105 3213 3385
Size: 1013 211 461 63 718 300 108 172
Cuts at: 3385 4028 4839 5222 5573 5998 6352 339
Size: 643 811 383 351 425 354 2851
Fragments arranged by size:
2851 1013 811 718 643 461 425 383
354 351 300 211 172 108 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 234 3260 5403 5589 6447 6580 7039 7248 234
Size: 3026 2143 186 858 133 459 209 1850
Fragments arranged by size:
3026 2143 1850 858 459 209 186 133
TatI w'GTAC_w
Cuts at: 1170 1442 3014 3278 4658 5312 8379 1170
Size: 272 1572 264 1380 654 3067 1655
Fragments arranged by size:
3067 1655 1572 1380 654 272 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 1330 1362 1509 1541 4816 4848 8381
Size: 32 667 32 147 32 3275 32 3533
Cuts at: 8381 8413 631
Size: 32 1082
Fragments arranged by size:
3533 3275 1082 667 147 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 762 2766 3000 4461 6661 762
Size: 2004 234 1461 2200 2965
Fragments arranged by size:
2965 2200 2004 1461 234
XbaI T'CTAG_A
Cuts at: 5895 5895
Size: 8864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1689 6359 1689
Size: 4670 4194
XhoI C'TCGA_G
Cuts at: 3962 3962
Size: 8864
XmaI C'CCGG_G
Cuts at: 194 1073 4149 4853 5078 5884 194
Size: 879 3076 704 225 806 3174
Fragments arranged by size:
3174 3076 879 806 704 225
ZraI GAC'GTC
Cuts at: 1341 5018 1341
Size: 3677 5187
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbsI BmgBI BmtI BsiWI BssHII
FseI FspAI HpaI NdeI NheI NotI NruI PacI
PshAI PspXI SacII SexAI SgrAI SrfI SwaI XmnI