(Circular) (Six-base) MAPSORT of: pF1KSDA0466.seq Check: 9930 from: 1 to: 6721 pF1KSDA0466 6721 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 839 1578 2157 2976 839 Size: 739 579 819 4584 Fragments arranged by size: 4584 819 739 579 AccI GT'mk_AC Cuts at: 3757 3757 Size: 6721 Acc65I G'GTAC_C Cuts at: 3404 3735 3404 Size: 331 6390 AclI AA'CG_TT Cuts at: 6561 6561 Size: 6721 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 439 1228 2338 2806 4544 4976 5725 5993 439 Size: 789 1110 468 1738 432 749 268 1167 Fragments arranged by size: 1738 1167 1110 789 749 468 432 268 AflII C'TTAA_G Cuts at: 1171 1171 Size: 6721 AflIII A'CryG_T Cuts at: 4071 5177 6154 4071 Size: 1106 977 4638 Fragments arranged by size: 4638 1106 977 AgeI A'CCGG_T Cuts at: 5109 5109 Size: 6721 AleI CACnn'nnGTG Cuts at: 2197 2197 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 587 587 Size: 6721 AlwNI CAG_nnn'CTG Cuts at: 588 771 3244 4024 5593 588 Size: 183 2473 780 1569 1716 Fragments arranged by size: 2473 1716 1569 780 183 ApaI G_GGCC'C Cuts at: 3238 3238 Size: 6721 ApaLI G'TGCA_C Cuts at: 5491 5491 Size: 6721 ApoI r'AATT_y Cuts at: 285 853 964 1141 1288 3723 6074 6330 285 Size: 568 111 177 147 2435 2351 256 676 Fragments arranged by size: 2435 2351 676 568 256 177 147 111 AseI AT'TA_AT Cuts at: 20 20 Size: 6721 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6721 AvaI C'yCGr_G Cuts at: 461 1922 3739 461 Size: 1461 1817 3443 Fragments arranged by size: 3443 1817 1461 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 199 232 199 Size: 33 6688 BamHI G'GATC_C Cuts at: 878 885 3744 878 Size: 7 2859 3855 Fragments arranged by size: 3855 2859 7 BanI G'GyrC_C Cuts at: 1525 3404 3615 3735 4149 4397 4432 1525 Size: 1879 211 120 414 248 35 3814 Fragments arranged by size: 3814 1879 414 248 211 120 35 BanII G_rGCy'C Cuts at: 232 620 2497 3238 3674 3733 4763 232 Size: 388 1877 741 436 59 1030 2190 Fragments arranged by size: 2190 1877 1030 741 436 388 59 BbeI G_GCGC'C Cuts at: 4153 4401 4153 Size: 248 6473 BbsI GAAGACnn'nnnn_ Cuts at: 2326 3504 2326 Size: 1178 5543 BciVI GTATCCnnnnn_n' Cuts at: 3697 4617 5380 6721 3697 Size: 920 763 1341 3697 Fragments arranged by size: 3697 1341 920 763 BclI T'GATC_A Cuts at: 4239 4239 Size: 6721 BfrBI ATG'CAT Cuts at: 1031 1031 Size: 6721 BglI GCCn_nnn'nGGC Cuts at: 2909 3260 2909 Size: 351 6370 BglII A'GATC_T Cuts at: 401 450 1211 4234 6273 401 Size: 49 761 3023 2039 849 Fragments arranged by size: 3023 2039 849 761 49 BlpI GC'TnA_GC Cuts at: 148 2491 2581 3226 3831 148 Size: 2343 90 645 605 3038 Fragments arranged by size: 3038 2343 645 605 90 Bme1580I G_kGCm'C Cuts at: 1891 3238 4344 4437 5495 1891 Size: 1347 1106 93 1058 3117 Fragments arranged by size: 3117 1347 1106 1058 93 BmrI ACTGGGnnnn_n' Cuts at: 1560 2900 3931 4012 4347 6493 1560 Size: 1340 1031 81 335 2146 1788 Fragments arranged by size: 2146 1788 1340 1031 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 651 729 2136 2401 5086 651 Size: 78 1407 265 2685 2286 Fragments arranged by size: 2685 2286 1407 265 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 634 726 1000 1057 1941 2152 2371 2632 2653 Size: 92 274 57 884 211 219 261 21 Cuts at: 2653 3048 3454 3570 3688 634 Size: 395 406 116 118 3667 Fragments arranged by size: 3667 884 406 395 274 261 219 211 118 116 92 57 21 Bpu10I CC'TnA_GC Cuts at: 718 2014 2711 718 Size: 1296 697 4728 Fragments arranged by size: 4728 1296 697 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1330 1414 2754 3895 4774 5268 5566 1330 Size: 84 1340 1141 879 494 298 2485 Fragments arranged by size: 2485 1340 1141 879 494 298 84 BsaI GGTCTCn'nnnn_ Cuts at: 634 2608 3021 6425 634 Size: 1974 413 3404 930 Fragments arranged by size: 3404 1974 930 413 BsaAI yAC'GTr Cuts at: 2475 3711 4072 2475 Size: 1236 361 5124 Fragments arranged by size: 5124 1236 361 BsaBI GATnn'nnATC Cuts at: 6 4233 6272 6 Size: 4227 2039 455 Fragments arranged by size: 4227 2039 455 BsaHI Gr'CG_yC Cuts at: 1574 2744 4150 4398 5102 6598 1574 Size: 1170 1406 248 704 1496 1697 Fragments arranged by size: 1697 1496 1406 1170 704 248 BsaWI w'CCGG_w Cuts at: 888 3908 4429 5109 5383 5530 5941 888 Size: 3020 521 680 274 147 411 1668 Fragments arranged by size: 3020 1668 680 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 510 540 923 953 1789 1819 1939 1969 3409 Size: 30 383 30 836 30 120 30 1440 Cuts at: 3409 3439 510 Size: 30 3792 Fragments arranged by size: 3792 1440 836 383 120 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2377 2380 2494 2539 2902 3181 3184 3187 3190 Size: 3 114 45 363 279 3 3 3 Cuts at: 3190 3193 3388 3433 3436 2377 Size: 3 195 45 3 5662 Fragments arranged by size: 5662 363 279 195 114 45 45 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 165 380 493 795 2669 2899 2941 3115 3385 Size: 215 113 302 1874 230 42 174 270 Cuts at: 3385 4144 4902 5481 5977 165 Size: 759 758 579 496 909 Fragments arranged by size: 1874 909 759 758 579 496 302 270 230 215 174 113 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 352 574 982 1552 1612 2875 352 Size: 222 408 570 60 1263 4198 Fragments arranged by size: 4198 1263 570 408 222 60 BsiEI CG_ry'CG Cuts at: 104 1059 4261 4307 5517 104 Size: 955 3202 46 1210 1308 Fragments arranged by size: 3202 1308 1210 955 46 BsiHKAI G_wGCw'C Cuts at: 620 2273 3311 3733 4511 4701 5495 620 Size: 1653 1038 422 778 190 794 1846 Fragments arranged by size: 1846 1653 1038 794 778 422 190 BsmI GAATG_Cn' Cuts at: 487 1660 3539 487 Size: 1173 1879 3669 Fragments arranged by size: 3669 1879 1173 BsmBI CGTCTCn'nnnn_ Cuts at: 2836 2836 Size: 6721 Bsp1286I G_dGCh'C Cuts at: 232 620 1891 2273 2497 3238 3311 3674 3733 Size: 388 1271 382 224 741 73 363 59 Cuts at: 3733 4344 4437 4511 4701 4763 5495 232 Size: 611 93 74 190 62 732 1458 Fragments arranged by size: 1458 1271 741 732 611 388 382 363 224 190 93 74 73 62 59 BspEI T'CCGG_A Cuts at: 888 5941 888 Size: 5053 1668 BspHI T'CATG_A Cuts at: 5897 5897 Size: 6721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 180 314 839 1415 1578 2135 2157 2613 2976 Size: 134 525 576 163 557 22 456 363 Cuts at: 2976 3769 3983 4285 4666 180 Size: 793 214 302 381 2235 Fragments arranged by size: 2235 793 576 557 525 456 381 363 302 214 163 134 22 BsrBI CCG'CTC Cuts at: 50 2715 3062 3213 5011 5065 6551 6716 50 Size: 2665 347 151 1798 54 1486 165 55 Fragments arranged by size: 2665 1798 1486 347 165 151 55 54 BsrDI GCAATG_nn' Cuts at: 258 1383 4631 258 Size: 1125 3248 2348 Fragments arranged by size: 3248 2348 1125 BsrFI r'CCGG_y Cuts at: 1014 1881 3314 3407 4717 4898 5109 1014 Size: 867 1433 93 1310 181 211 2626 Fragments arranged by size: 2626 1433 1310 867 211 181 93 BssSI C'ACGA_G Cuts at: 4990 5350 4990 Size: 360 6361 BstAPI GCAn_nnn'nTGC Cuts at: 4096 4096 Size: 6721 BstBI TT'CG_AA Cuts at: 110 110 Size: 6721 BstEII G'GTnAC_C Cuts at: 192 1791 1911 192 Size: 1599 120 5002 Fragments arranged by size: 5002 1599 120 BstXI CCAn_nnnn'nTGG Cuts at: 2130 3561 2130 Size: 1431 5290 BstYI r'GATC_y Cuts at: 1 401 450 878 885 1211 3744 4211 4234 Size: 400 49 428 7 326 2533 467 23 Cuts at: 4234 4569 4815 5829 5915 5927 6273 1 Size: 335 246 1014 86 12 346 449 Fragments arranged by size: 2533 1014 467 449 428 400 346 335 326 246 86 49 23 12 7 Bsu36I CC'TnA_GG Cuts at: 2575 3103 2575 Size: 528 6193 BtgI C'CryG_G Cuts at: 347 390 701 935 1106 1361 3011 347 Size: 43 311 234 171 255 1650 4057 Fragments arranged by size: 4057 1650 311 255 234 171 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1083 4669 4850 5013 1083 Size: 3586 181 163 2791 Fragments arranged by size: 3586 2791 181 163 BtsI GCAGTG_nn' Cuts at: 569 1428 1688 1825 3962 569 Size: 859 260 137 2137 3328 Fragments arranged by size: 3328 2137 859 260 137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 240 275 240 Size: 35 6686 DraI TTT'AAA Cuts at: 3718 3718 Size: 6721 DraIII CAC_nnn'GTG Cuts at: 597 1005 597 Size: 408 6313 DrdI GACnn_nn'nnGTC Cuts at: 1933 2998 4425 5285 1933 Size: 1065 1427 860 3369 Fragments arranged by size: 3369 1427 1065 860 EaeI y'GGCC_r Cuts at: 1178 1370 4304 4478 4869 4896 6243 1178 Size: 192 2934 174 391 27 1347 1656 Fragments arranged by size: 2934 1656 1347 391 192 174 27 EagI C'GGCC_G Cuts at: 4304 4304 Size: 6721 EarI CTCTTCn'nnn_ Cuts at: 360 2176 2839 3149 3505 4742 4952 360 Size: 1816 663 310 356 1237 210 2129 Fragments arranged by size: 2129 1816 1237 663 356 310 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 802 1213 1999 2091 2125 2325 4927 5239 5385 Size: 411 786 92 34 200 2602 312 146 Cuts at: 5385 6531 802 Size: 1146 992 Fragments arranged by size: 2602 1146 992 786 411 312 200 146 92 34 EcoICRI GAG'CTC Cuts at: 618 3731 618 Size: 3113 3608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 439 634 726 1000 1057 1228 1941 2152 2338 Size: 195 92 274 57 171 713 211 186 Cuts at: 2338 2371 2632 2653 2806 3048 3454 3570 3688 Size: 33 261 21 153 242 406 116 118 Cuts at: 3688 4544 4976 5725 5993 439 Size: 856 432 749 268 1167 Fragments arranged by size: 1167 856 749 713 432 406 274 268 261 242 211 195 186 171 153 118 116 92 57 33 21 EcoNI CCTnn'n_nnAGG Cuts at: 775 1225 775 Size: 450 6271 EcoO109I rG'GnC_Cy Cuts at: 208 271 606 1111 1404 1851 1952 2117 2186 Size: 63 335 505 293 447 101 165 69 Cuts at: 2186 2370 2412 2867 3858 208 Size: 184 42 455 991 3071 Fragments arranged by size: 3071 991 505 455 447 335 293 184 165 101 69 63 42 EcoRI G'AATT_C Cuts at: 853 964 1288 3723 853 Size: 111 324 2435 3851 Fragments arranged by size: 3851 2435 324 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 528 4738 528 Size: 4210 2511 FspI TGC'GCA Cuts at: 4500 4500 Size: 6721 HaeII r_GCGC'y Cuts at: 1716 4153 4401 5425 6404 1716 Size: 2437 248 1024 979 2033 Fragments arranged by size: 2437 2033 1024 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 386 418 870 902 923 955 1296 1328 1939 Size: 32 452 32 21 32 341 32 611 Cuts at: 1939 1971 2429 2461 3409 3441 4561 4591 4593 Size: 32 458 32 948 32 1120 30 2 Cuts at: 4593 4623 386 Size: 30 2484 Fragments arranged by size: 2484 1120 948 611 458 452 341 32 32 32 32 32 32 32 30 30 21 2 HincII GTy'rAC Cuts at: 942 3758 942 Size: 2816 3905 HindIII A'AGCT_T Cuts at: 2308 2308 Size: 6721 KasI G'GCGC_C Cuts at: 4149 4397 4149 Size: 248 6473 KpnI G_GTAC'C Cuts at: 3408 3739 3408 Size: 331 6390 MluI A'CGCG_T Cuts at: 6154 6154 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2147 3512 5392 5576 2147 Size: 1365 1880 184 3292 Fragments arranged by size: 3292 1880 1365 184 MscI TGG'CCA Cuts at: 1180 1372 4480 1180 Size: 192 3108 3421 Fragments arranged by size: 3421 3108 192 MslI CAynn'nnrTG Cuts at: 574 1360 2197 4708 4835 574 Size: 786 837 2511 127 2460 Fragments arranged by size: 2511 2460 837 786 127 MspA1I CmG'CkG Cuts at: 186 392 612 2456 2631 2864 2876 2941 3039 Size: 206 220 1844 175 233 12 65 98 Cuts at: 3039 3830 4144 4504 5519 5764 186 Size: 791 314 360 1015 245 1143 Fragments arranged by size: 1844 1143 1015 791 360 314 245 233 220 206 175 98 65 12 NaeI GCC'GGC Cuts at: 4900 4900 Size: 6721 NarI GG'CG_CC Cuts at: 4150 4398 4150 Size: 248 6473 NcoI C'CATG_G Cuts at: 347 1106 347 Size: 759 5962 NgoMIV G'CCGG_C Cuts at: 4898 4898 Size: 6721 NruI TCG'CGA Cuts at: 5082 5082 Size: 6721 NsiI A_TGCA'T Cuts at: 1033 1033 Size: 6721 NspI r_CATG'y Cuts at: 2404 2916 3772 4272 5181 2404 Size: 512 856 500 909 3944 Fragments arranged by size: 3944 909 856 512 500 PasI CC'CwG_GG Cuts at: 2592 2592 Size: 6721 PciI A'CATG_T Cuts at: 5177 5177 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1884 1908 2061 2676 2847 1884 Size: 24 153 615 171 5758 Fragments arranged by size: 5758 615 171 153 24 PfoI T'CCnGG_A Cuts at: 881 1034 1777 881 Size: 153 743 5825 Fragments arranged by size: 5825 743 153 PmeI GTTT'AAAC Cuts at: 3718 3718 Size: 6721 PmlI CAC'GTG Cuts at: 2475 2475 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5860 5892 5860 Size: 32 6689 PpuMI rG'GwC_Cy Cuts at: 208 271 606 1111 1404 1851 1952 2117 2186 Size: 63 335 505 293 447 101 165 69 Cuts at: 2186 2867 208 Size: 681 4062 Fragments arranged by size: 4062 681 505 447 335 293 165 101 69 63 PspOMI G'GGCC_C Cuts at: 3234 3234 Size: 6721 PstI C_TGCA'G Cuts at: 566 836 1423 2150 2168 2987 3332 3766 4451 Size: 270 587 727 18 819 345 434 685 Cuts at: 4451 566 Size: 2836 Fragments arranged by size: 2836 819 727 685 587 434 345 270 18 PvuI CG_AT'CG Cuts at: 104 104 Size: 6721 PvuII CAG'CTG Cuts at: 2631 2876 3039 4144 4504 2631 Size: 245 163 1105 360 4848 Fragments arranged by size: 4848 1105 360 245 163 RsrII CG'GwC_CG Cuts at: 4914 4914 Size: 6721 SacI G_AGCT'C Cuts at: 620 3733 620 Size: 3113 3608 SacII CC_GC'GG Cuts at: 393 393 Size: 6721 SalI G'TCGA_C Cuts at: 3756 3756 Size: 6721 SanDI GG'GwC_CC Cuts at: 1851 1851 Size: 6721 SapI GCTCTTCn'nnn_ Cuts at: 4742 4952 4742 Size: 210 6511 SbfI CC_TGCA'GG Cuts at: 2987 3766 2987 Size: 779 5942 ScaI AGT'ACT Cuts at: 6238 6238 Size: 6721 SfcI C'TryA_G Cuts at: 33 562 832 1419 1797 2146 2164 2871 2983 Size: 529 270 587 378 349 18 707 112 Cuts at: 2983 3045 3328 3762 4447 5442 5633 33 Size: 62 283 434 685 995 191 1121 Fragments arranged by size: 1121 995 707 685 587 529 434 378 349 283 270 191 112 62 18 SfoI GGC'GCC Cuts at: 4151 4399 4151 Size: 248 6473 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6721 SmaI CCC'GGG Cuts at: 463 1924 3741 463 Size: 1461 1817 3443 Fragments arranged by size: 3443 1817 1461 SmlI C'TyrA_G Cuts at: 1171 1345 1393 2733 3874 4789 5283 5545 1171 Size: 174 48 1340 1141 915 494 262 2347 Fragments arranged by size: 2347 1340 1141 915 494 262 174 48 SnaBI TAC'GTA Cuts at: 3711 3711 Size: 6721 SpeI A'CTAG_T Cuts at: 67 67 Size: 6721 SphI G_CATG'C Cuts at: 3772 4272 3772 Size: 500 6221 StyI C'CwwG_G Cuts at: 347 1106 3268 3853 4207 347 Size: 759 2162 585 354 2861 Fragments arranged by size: 2861 2162 759 585 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 157 394 507 1662 2341 4302 4435 4894 5105 Size: 237 113 1155 679 1961 133 459 211 Cuts at: 5105 157 Size: 1773 Fragments arranged by size: 1961 1773 1155 679 459 237 211 133 113 TatI w'GTAC_w Cuts at: 6236 6236 Size: 6721 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 510 542 883 915 1095 1127 1678 1710 6238 Size: 32 341 32 180 32 551 32 4528 Cuts at: 6238 6270 510 Size: 32 961 Fragments arranged by size: 4528 961 551 341 180 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1195 4516 1195 Size: 3321 3400 XbaI T'CTAG_A Cuts at: 3450 3750 3450 Size: 300 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 889 4214 889 Size: 3325 3396 XmaI C'CCGG_G Cuts at: 461 1922 3739 461 Size: 1461 1817 3443 Fragments arranged by size: 3443 1817 1461 XmnI GAAnn'nnTTC Cuts at: 403 921 403 Size: 518 6203 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AhdI AloI AscI AvrII BbvCI BcgI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI PacI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI