Restriction map for
pF1KSDA0466
(Circular) (Six-base) MAPSORT of: pF1KSDA0466.seq Check: 9930 from: 1 to: 6721
pF1KSDA0466 6721 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 839 1578 2157 2976 839
Size: 739 579 819 4584
Fragments arranged by size:
4584 819 739 579
AccI GT'mk_AC
Cuts at: 3757 3757
Size: 6721
Acc65I G'GTAC_C
Cuts at: 3404 3735 3404
Size: 331 6390
AclI AA'CG_TT
Cuts at: 6561 6561
Size: 6721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1228 2338 2806 4544 4976 5725 5993 439
Size: 789 1110 468 1738 432 749 268 1167
Fragments arranged by size:
1738 1167 1110 789 749 468 432 268
AflII C'TTAA_G
Cuts at: 1171 1171
Size: 6721
AflIII A'CryG_T
Cuts at: 4071 5177 6154 4071
Size: 1106 977 4638
Fragments arranged by size:
4638 1106 977
AgeI A'CCGG_T
Cuts at: 5109 5109
Size: 6721
AleI CACnn'nnGTG
Cuts at: 2197 2197
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 588 771 3244 4024 5593 588
Size: 183 2473 780 1569 1716
Fragments arranged by size:
2473 1716 1569 780 183
ApaI G_GGCC'C
Cuts at: 3238 3238
Size: 6721
ApaLI G'TGCA_C
Cuts at: 5491 5491
Size: 6721
ApoI r'AATT_y
Cuts at: 285 853 964 1141 1288 3723 6074 6330 285
Size: 568 111 177 147 2435 2351 256 676
Fragments arranged by size:
2435 2351 676 568 256 177 147 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 6721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6721
AvaI C'yCGr_G
Cuts at: 461 1922 3739 461
Size: 1461 1817 3443
Fragments arranged by size:
3443 1817 1461
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 199 232 199
Size: 33 6688
BamHI G'GATC_C
Cuts at: 878 885 3744 878
Size: 7 2859 3855
Fragments arranged by size:
3855 2859 7
BanI G'GyrC_C
Cuts at: 1525 3404 3615 3735 4149 4397 4432 1525
Size: 1879 211 120 414 248 35 3814
Fragments arranged by size:
3814 1879 414 248 211 120 35
BanII G_rGCy'C
Cuts at: 232 620 2497 3238 3674 3733 4763 232
Size: 388 1877 741 436 59 1030 2190
Fragments arranged by size:
2190 1877 1030 741 436 388 59
BbeI G_GCGC'C
Cuts at: 4153 4401 4153
Size: 248 6473
BbsI GAAGACnn'nnnn_
Cuts at: 2326 3504 2326
Size: 1178 5543
BciVI GTATCCnnnnn_n'
Cuts at: 3697 4617 5380 6721 3697
Size: 920 763 1341 3697
Fragments arranged by size:
3697 1341 920 763
BclI T'GATC_A
Cuts at: 4239 4239
Size: 6721
BfrBI ATG'CAT
Cuts at: 1031 1031
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 2909 3260 2909
Size: 351 6370
BglII A'GATC_T
Cuts at: 401 450 1211 4234 6273 401
Size: 49 761 3023 2039 849
Fragments arranged by size:
3023 2039 849 761 49
BlpI GC'TnA_GC
Cuts at: 148 2491 2581 3226 3831 148
Size: 2343 90 645 605 3038
Fragments arranged by size:
3038 2343 645 605 90
Bme1580I G_kGCm'C
Cuts at: 1891 3238 4344 4437 5495 1891
Size: 1347 1106 93 1058 3117
Fragments arranged by size:
3117 1347 1106 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1560 2900 3931 4012 4347 6493 1560
Size: 1340 1031 81 335 2146 1788
Fragments arranged by size:
2146 1788 1340 1031 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 651 729 2136 2401 5086 651
Size: 78 1407 265 2685 2286
Fragments arranged by size:
2685 2286 1407 265 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 726 1000 1057 1941 2152 2371 2632 2653
Size: 92 274 57 884 211 219 261 21
Cuts at: 2653 3048 3454 3570 3688 634
Size: 395 406 116 118 3667
Fragments arranged by size:
3667 884 406 395 274 261 219 211
118 116 92 57 21
Bpu10I CC'TnA_GC
Cuts at: 718 2014 2711 718
Size: 1296 697 4728
Fragments arranged by size:
4728 1296 697
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1414 2754 3895 4774 5268 5566 1330
Size: 84 1340 1141 879 494 298 2485
Fragments arranged by size:
2485 1340 1141 879 494 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 634 2608 3021 6425 634
Size: 1974 413 3404 930
Fragments arranged by size:
3404 1974 930 413
BsaAI yAC'GTr
Cuts at: 2475 3711 4072 2475
Size: 1236 361 5124
Fragments arranged by size:
5124 1236 361
BsaBI GATnn'nnATC
Cuts at: 6 4233 6272 6
Size: 4227 2039 455
Fragments arranged by size:
4227 2039 455
BsaHI Gr'CG_yC
Cuts at: 1574 2744 4150 4398 5102 6598 1574
Size: 1170 1406 248 704 1496 1697
Fragments arranged by size:
1697 1496 1406 1170 704 248
BsaWI w'CCGG_w
Cuts at: 888 3908 4429 5109 5383 5530 5941 888
Size: 3020 521 680 274 147 411 1668
Fragments arranged by size:
3020 1668 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 510 540 923 953 1789 1819 1939 1969 3409
Size: 30 383 30 836 30 120 30 1440
Cuts at: 3409 3439 510
Size: 30 3792
Fragments arranged by size:
3792 1440 836 383 120 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2377 2380 2494 2539 2902 3181 3184 3187 3190
Size: 3 114 45 363 279 3 3 3
Cuts at: 3190 3193 3388 3433 3436 2377
Size: 3 195 45 3 5662
Fragments arranged by size:
5662 363 279 195 114 45 45 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 165 380 493 795 2669 2899 2941 3115 3385
Size: 215 113 302 1874 230 42 174 270
Cuts at: 3385 4144 4902 5481 5977 165
Size: 759 758 579 496 909
Fragments arranged by size:
1874 909 759 758 579 496 302 270
230 215 174 113 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 574 982 1552 1612 2875 352
Size: 222 408 570 60 1263 4198
Fragments arranged by size:
4198 1263 570 408 222 60
BsiEI CG_ry'CG
Cuts at: 104 1059 4261 4307 5517 104
Size: 955 3202 46 1210 1308
Fragments arranged by size:
3202 1308 1210 955 46
BsiHKAI G_wGCw'C
Cuts at: 620 2273 3311 3733 4511 4701 5495 620
Size: 1653 1038 422 778 190 794 1846
Fragments arranged by size:
1846 1653 1038 794 778 422 190
BsmI GAATG_Cn'
Cuts at: 487 1660 3539 487
Size: 1173 1879 3669
Fragments arranged by size:
3669 1879 1173
BsmBI CGTCTCn'nnnn_
Cuts at: 2836 2836
Size: 6721
Bsp1286I G_dGCh'C
Cuts at: 232 620 1891 2273 2497 3238 3311 3674 3733
Size: 388 1271 382 224 741 73 363 59
Cuts at: 3733 4344 4437 4511 4701 4763 5495 232
Size: 611 93 74 190 62 732 1458
Fragments arranged by size:
1458 1271 741 732 611 388 382 363
224 190 93 74 73 62 59
BspEI T'CCGG_A
Cuts at: 888 5941 888
Size: 5053 1668
BspHI T'CATG_A
Cuts at: 5897 5897
Size: 6721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 314 839 1415 1578 2135 2157 2613 2976
Size: 134 525 576 163 557 22 456 363
Cuts at: 2976 3769 3983 4285 4666 180
Size: 793 214 302 381 2235
Fragments arranged by size:
2235 793 576 557 525 456 381 363
302 214 163 134 22
BsrBI CCG'CTC
Cuts at: 50 2715 3062 3213 5011 5065 6551 6716 50
Size: 2665 347 151 1798 54 1486 165 55
Fragments arranged by size:
2665 1798 1486 347 165 151 55 54
BsrDI GCAATG_nn'
Cuts at: 258 1383 4631 258
Size: 1125 3248 2348
Fragments arranged by size:
3248 2348 1125
BsrFI r'CCGG_y
Cuts at: 1014 1881 3314 3407 4717 4898 5109 1014
Size: 867 1433 93 1310 181 211 2626
Fragments arranged by size:
2626 1433 1310 867 211 181 93
BssSI C'ACGA_G
Cuts at: 4990 5350 4990
Size: 360 6361
BstAPI GCAn_nnn'nTGC
Cuts at: 4096 4096
Size: 6721
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6721
BstEII G'GTnAC_C
Cuts at: 192 1791 1911 192
Size: 1599 120 5002
Fragments arranged by size:
5002 1599 120
BstXI CCAn_nnnn'nTGG
Cuts at: 2130 3561 2130
Size: 1431 5290
BstYI r'GATC_y
Cuts at: 1 401 450 878 885 1211 3744 4211 4234
Size: 400 49 428 7 326 2533 467 23
Cuts at: 4234 4569 4815 5829 5915 5927 6273 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
2533 1014 467 449 428 400 346 335
326 246 86 49 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 2575 3103 2575
Size: 528 6193
BtgI C'CryG_G
Cuts at: 347 390 701 935 1106 1361 3011 347
Size: 43 311 234 171 255 1650 4057
Fragments arranged by size:
4057 1650 311 255 234 171 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1083 4669 4850 5013 1083
Size: 3586 181 163 2791
Fragments arranged by size:
3586 2791 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1428 1688 1825 3962 569
Size: 859 260 137 2137 3328
Fragments arranged by size:
3328 2137 859 260 137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 240 275 240
Size: 35 6686
DraI TTT'AAA
Cuts at: 3718 3718
Size: 6721
DraIII CAC_nnn'GTG
Cuts at: 597 1005 597
Size: 408 6313
DrdI GACnn_nn'nnGTC
Cuts at: 1933 2998 4425 5285 1933
Size: 1065 1427 860 3369
Fragments arranged by size:
3369 1427 1065 860
EaeI y'GGCC_r
Cuts at: 1178 1370 4304 4478 4869 4896 6243 1178
Size: 192 2934 174 391 27 1347 1656
Fragments arranged by size:
2934 1656 1347 391 192 174 27
EagI C'GGCC_G
Cuts at: 4304 4304
Size: 6721
EarI CTCTTCn'nnn_
Cuts at: 360 2176 2839 3149 3505 4742 4952 360
Size: 1816 663 310 356 1237 210 2129
Fragments arranged by size:
2129 1816 1237 663 356 310 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 802 1213 1999 2091 2125 2325 4927 5239 5385
Size: 411 786 92 34 200 2602 312 146
Cuts at: 5385 6531 802
Size: 1146 992
Fragments arranged by size:
2602 1146 992 786 411 312 200 146
92 34
EcoICRI GAG'CTC
Cuts at: 618 3731 618
Size: 3113 3608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 634 726 1000 1057 1228 1941 2152 2338
Size: 195 92 274 57 171 713 211 186
Cuts at: 2338 2371 2632 2653 2806 3048 3454 3570 3688
Size: 33 261 21 153 242 406 116 118
Cuts at: 3688 4544 4976 5725 5993 439
Size: 856 432 749 268 1167
Fragments arranged by size:
1167 856 749 713 432 406 274 268
261 242 211 195 186 171 153 118
116 92 57 33 21
EcoNI CCTnn'n_nnAGG
Cuts at: 775 1225 775
Size: 450 6271
EcoO109I rG'GnC_Cy
Cuts at: 208 271 606 1111 1404 1851 1952 2117 2186
Size: 63 335 505 293 447 101 165 69
Cuts at: 2186 2370 2412 2867 3858 208
Size: 184 42 455 991 3071
Fragments arranged by size:
3071 991 505 455 447 335 293 184
165 101 69 63 42
EcoRI G'AATT_C
Cuts at: 853 964 1288 3723 853
Size: 111 324 2435 3851
Fragments arranged by size:
3851 2435 324 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 528 4738 528
Size: 4210 2511
FspI TGC'GCA
Cuts at: 4500 4500
Size: 6721
HaeII r_GCGC'y
Cuts at: 1716 4153 4401 5425 6404 1716
Size: 2437 248 1024 979 2033
Fragments arranged by size:
2437 2033 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 386 418 870 902 923 955 1296 1328 1939
Size: 32 452 32 21 32 341 32 611
Cuts at: 1939 1971 2429 2461 3409 3441 4561 4591 4593
Size: 32 458 32 948 32 1120 30 2
Cuts at: 4593 4623 386
Size: 30 2484
Fragments arranged by size:
2484 1120 948 611 458 452 341 32
32 32 32 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 942 3758 942
Size: 2816 3905
HindIII A'AGCT_T
Cuts at: 2308 2308
Size: 6721
KasI G'GCGC_C
Cuts at: 4149 4397 4149
Size: 248 6473
KpnI G_GTAC'C
Cuts at: 3408 3739 3408
Size: 331 6390
MluI A'CGCG_T
Cuts at: 6154 6154
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2147 3512 5392 5576 2147
Size: 1365 1880 184 3292
Fragments arranged by size:
3292 1880 1365 184
MscI TGG'CCA
Cuts at: 1180 1372 4480 1180
Size: 192 3108 3421
Fragments arranged by size:
3421 3108 192
MslI CAynn'nnrTG
Cuts at: 574 1360 2197 4708 4835 574
Size: 786 837 2511 127 2460
Fragments arranged by size:
2511 2460 837 786 127
MspA1I CmG'CkG
Cuts at: 186 392 612 2456 2631 2864 2876 2941 3039
Size: 206 220 1844 175 233 12 65 98
Cuts at: 3039 3830 4144 4504 5519 5764 186
Size: 791 314 360 1015 245 1143
Fragments arranged by size:
1844 1143 1015 791 360 314 245 233
220 206 175 98 65 12
NaeI GCC'GGC
Cuts at: 4900 4900
Size: 6721
NarI GG'CG_CC
Cuts at: 4150 4398 4150
Size: 248 6473
NcoI C'CATG_G
Cuts at: 347 1106 347
Size: 759 5962
NgoMIV G'CCGG_C
Cuts at: 4898 4898
Size: 6721
NruI TCG'CGA
Cuts at: 5082 5082
Size: 6721
NsiI A_TGCA'T
Cuts at: 1033 1033
Size: 6721
NspI r_CATG'y
Cuts at: 2404 2916 3772 4272 5181 2404
Size: 512 856 500 909 3944
Fragments arranged by size:
3944 909 856 512 500
PasI CC'CwG_GG
Cuts at: 2592 2592
Size: 6721
PciI A'CATG_T
Cuts at: 5177 5177
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1884 1908 2061 2676 2847 1884
Size: 24 153 615 171 5758
Fragments arranged by size:
5758 615 171 153 24
PfoI T'CCnGG_A
Cuts at: 881 1034 1777 881
Size: 153 743 5825
Fragments arranged by size:
5825 743 153
PmeI GTTT'AAAC
Cuts at: 3718 3718
Size: 6721
PmlI CAC'GTG
Cuts at: 2475 2475
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5860 5892 5860
Size: 32 6689
PpuMI rG'GwC_Cy
Cuts at: 208 271 606 1111 1404 1851 1952 2117 2186
Size: 63 335 505 293 447 101 165 69
Cuts at: 2186 2867 208
Size: 681 4062
Fragments arranged by size:
4062 681 505 447 335 293 165 101
69 63
PspOMI G'GGCC_C
Cuts at: 3234 3234
Size: 6721
PstI C_TGCA'G
Cuts at: 566 836 1423 2150 2168 2987 3332 3766 4451
Size: 270 587 727 18 819 345 434 685
Cuts at: 4451 566
Size: 2836
Fragments arranged by size:
2836 819 727 685 587 434 345 270
18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6721
PvuII CAG'CTG
Cuts at: 2631 2876 3039 4144 4504 2631
Size: 245 163 1105 360 4848
Fragments arranged by size:
4848 1105 360 245 163
RsrII CG'GwC_CG
Cuts at: 4914 4914
Size: 6721
SacI G_AGCT'C
Cuts at: 620 3733 620
Size: 3113 3608
SacII CC_GC'GG
Cuts at: 393 393
Size: 6721
SalI G'TCGA_C
Cuts at: 3756 3756
Size: 6721
SanDI GG'GwC_CC
Cuts at: 1851 1851
Size: 6721
SapI GCTCTTCn'nnn_
Cuts at: 4742 4952 4742
Size: 210 6511
SbfI CC_TGCA'GG
Cuts at: 2987 3766 2987
Size: 779 5942
ScaI AGT'ACT
Cuts at: 6238 6238
Size: 6721
SfcI C'TryA_G
Cuts at: 33 562 832 1419 1797 2146 2164 2871 2983
Size: 529 270 587 378 349 18 707 112
Cuts at: 2983 3045 3328 3762 4447 5442 5633 33
Size: 62 283 434 685 995 191 1121
Fragments arranged by size:
1121 995 707 685 587 529 434 378
349 283 270 191 112 62 18
SfoI GGC'GCC
Cuts at: 4151 4399 4151
Size: 248 6473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6721
SmaI CCC'GGG
Cuts at: 463 1924 3741 463
Size: 1461 1817 3443
Fragments arranged by size:
3443 1817 1461
SmlI C'TyrA_G
Cuts at: 1171 1345 1393 2733 3874 4789 5283 5545 1171
Size: 174 48 1340 1141 915 494 262 2347
Fragments arranged by size:
2347 1340 1141 915 494 262 174 48
SnaBI TAC'GTA
Cuts at: 3711 3711
Size: 6721
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6721
SphI G_CATG'C
Cuts at: 3772 4272 3772
Size: 500 6221
StyI C'CwwG_G
Cuts at: 347 1106 3268 3853 4207 347
Size: 759 2162 585 354 2861
Fragments arranged by size:
2861 2162 759 585 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 394 507 1662 2341 4302 4435 4894 5105
Size: 237 113 1155 679 1961 133 459 211
Cuts at: 5105 157
Size: 1773
Fragments arranged by size:
1961 1773 1155 679 459 237 211 133
113
TatI w'GTAC_w
Cuts at: 6236 6236
Size: 6721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 510 542 883 915 1095 1127 1678 1710 6238
Size: 32 341 32 180 32 551 32 4528
Cuts at: 6238 6270 510
Size: 32 961
Fragments arranged by size:
4528 961 551 341 180 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1195 4516 1195
Size: 3321 3400
XbaI T'CTAG_A
Cuts at: 3450 3750 3450
Size: 300 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 889 4214 889
Size: 3325 3396
XmaI C'CCGG_G
Cuts at: 461 1922 3739 461
Size: 1461 1817 3443
Fragments arranged by size:
3443 1817 1461
XmnI GAAnn'nnTTC
Cuts at: 403 921 403
Size: 518 6203
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BbvCI BcgI
BmgBI BmtI BsiWI BsrGI BssHII BstZ17I ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI PacI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI